| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Research Abstract |
We isolated upstream region of cuticle protein genes BMWCP2 and BMWCP5 and clarified the ecdysone responsive sequence. We constructed the method of the introduction of DNA into wing discs and wing tissues by a gene gun. We clarified that BR-C Z2, which is an early ecdysone responsive gene, bound and activated the promoter activity the BMWCP10. We found EcRE upstream of BMWCP10 and showed the binding of EcR to EcRE by a gel electrophoresis mobility sift assay and a reporter assay. We found that the different transcription factors were expressed in different region of the epidermis.
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