Identification of ecdysteroidogenic enzymes and regulation of their gene expressions by neuropeptides.

• Project/Area Number: 19380034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: The University of Tokyo
• Principal Investigator: KATAOKA Hiroshi The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (60202008)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: エクジソン / ステロイド / 昆虫 / 神経ペプチド / シトクロームP450 / 生合成

Research Abstract

To elucidate the mechanism of molting and metamorphosis in insect's growth process, we tried to identify the biosynthetic enzymes of the molting hormone, ecdysone, the neuropeptides which controlled biosynthetic rate of ecdysone and their receptor. As a result, one candidate of biosynthetic enzyme, two neuropeptides and one neuropeptide receptor were identified. In addition, it was found that each enzyme gene expression was positively or negatively controlled by different neuropeptides.

Research Products

• [Journal Article] Bombyx orcokinins are brain-gut peptides involved in the neuronal regulation of ecdysteroidogenesis (2011)
  • Author(s): N.Yamanaka, L.Roller, D.Zitnan, H.Satake, A.Mizoguchi, H.Kataoka, Y.Tanaka.
  • Journal Title: J.Comp.Neurology 519 Pages: 238-246
• [Journal Article] Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis (2010)
  • Author(s): N.Yamanaka, Y-J.Hua, L.Roller, I.Spalovska Valachova, A.Mizoguchi, H.Kataoka, Y.Tanaka.
  • Journal Title: Proc.Natl.Acd.Sci.USA 107 Pages: 2060-2065
• [Journal Article] Bombyx prothoracicostatic peptides activate the sex peptide receptor toregulate ecdysteroid biosynthesis. (2010)
  • Author(s): N.Yamanaka, Y-J.Hua, L.Roller, I.Spalovska-Valachova, A.Mizoguchi, H.Kataoka, Y.Tanaka.
  • Journal Title: Proc.Natl.Acd.Sci.USA. 107 Pages: 2060-2065
  • Peer Reviewed
• [Journal Article] A basic-HLH transcription factor HLH54F is highly expressed in prothoracic gland in the silkworm Bombyx mori and the fruit fly Drosophila melanogaster (2009)
  • Author(s): T.Namiki, R.Niwa, A.Higuchi, T.Yoshiyama, K.Mita, H.Kataoka.
  • Journal Title: Biosci.Biothech.Biochem 73 Pages: 762-765
  • NAID: 10027539262
• [Journal Article] A basic-HLH transcription factor HLH54F is highly expressed in prothoracic、gland in the silkworm Bombyx mori and the frui fly Drosophila melanogaster. (2009)
  • Author(s): T. Namiki et al.
  • Journal Title: Biosci. Biothech. Biochem. 73 Pages: 762-765
  • Peer Reviewed
• [Journal Article] Neuropeptide receptor transcriptome reveals unidentified neuroendocrine pathway (2008)
  • Author(s): N.Yamanaka, S.Yamamoto, D.Zitnan, K.Watanabe, T.Kawada, H.Satake, Y.Kaneko,K.Hiruma, Y.Tanaka, T.Shinoda, H.Kataoka.
  • Journal Title: PLoS ONE 3
• [Journal Article] The unique evolution of neuropeptide genes in the silkworm Bombyx mori (2008)
  • Author(s): L.Roller, N.Yamanaka, K.Watanabe, I.Daubnerova, D.Zitnan, H.Kataoka, Y.Tanaka.
  • Journal Title: Insect Biochem.Mol.Biol 38 Pages: 1147-1157
• [Journal Article] The genome of a lepidopteran model insect, the silkworm Bombyx mori (2008)
  • Author(s): The International Silkworm Genome Consortium.
  • Journal Title: Insect Biochem.Mol.Biol 38 Pages: 1036-1045
• [Journal Article] The unique evolution of neuropeptide genes in the silkworm Bombyx mori. (2008)
  • Author(s): L. Roller et al.
  • Journal Title: Insect Biochem. Mol. Biol. 38 Pages: 1147-1157
  • Peer Reviewed
• [Journal Article] Neuropeptide receptor transcriptome reveals unidentified neuroendocrine pathway (2008)
  • Author(s): N. Yamanaka et al.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Differential regulation of Ecdysteroidogenic P450 gene expression in the silkworm, Bombyx mori. Biosci. Biothech. Biochem (2008)
  • Author(s): N. Yamanaka, N. Honda, N. Osato, R. Niwa, A. Mizoguchi, H. Kataoka
  • Journal Title: Biosci. Biothech. Biochem. 71 Pages: 2808-2814
  • Peer Reviewed
• [Journal Article] FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori (2007)
  • Author(s): k.Watanabe, J.J.Hull, T.Niimi, K.Imai, S. Matsumoto, T.Yaginuma, H.Kataoka.
  • Journal Title: Mol.Cell.Endocrinol 273 Pages: 51-58
• [Journal Article] Differential regulation of Ecdysteroidogenic P450 gene expression in the silkworm (2007)
  • Author(s): N.Yamanaka, N.Honda, N.Osato, R.Niwa, A.Mizoguchi, H.Kataoka.
  • Journal Title: Bombyx mori.Biosci.Biothech.Biochem 71 Pages: 2808-2814
• [Journal Article] FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori (2007)
  • Author(s): K. Watanabe, J. J. Hull, T. Niimi, K. Imai, S. Matsumoto, T. Yaginuma, H. Kataoka
  • Journal Title: Mol. Cell. Endocrinol. 273 Pages: 51-58
  • Peer Reviewed
• [Presentation] ショウジョウバエMAPRファミリーCG9066とエクジソン生合成酵素との相互作用. (2010)
  • Author(s): 山田哲也、中岡貴義、藤本善徳、片岡宏誌
  • Organizer: 2010 年度日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] ショウジョウバエMAPRファミリーCG9066とエクジソン生合成酵素との相互作用 (2010)
  • Author(s): 山田哲也、中岡貴義、藤本善徳、片岡宏誌
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] Neverland, an evolutionarily conserved Rieske-type family of proteins, is essential for steroid hormone biosynthesis and regulating cholesterol metabolism during development. (2009)
  • Author(s): T.Yoshiyama, K.Hada, S.Enya, Y.Sasakura, K.Shiomi, 片岡宏誌, 丹羽隆介
  • Organizer: 日本発生生物学会第42 回大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-28
• [Presentation] 前胸腺におけるエクジソン生合成と神経ペプチド類による調節 (2009)
  • Author(s): 片岡宏誌
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 昆虫前胸腺におけるエクジソン生合成酵素遺伝子の探 索 (2009)
  • Author(s): 松谷武嗣、丹羽隆介、葛西真治、冨田隆史、三田和英、片岡宏誌
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 昆虫前胸腺におけるエクジソン生合成酵素遺伝子の探索 (2009)
  • Author(s): 松谷武嗣他
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] Cytochrome P450s involved in biosynthesis of ecdysteroids in insects ; Regulation of gene expression by neuropeptides (2009)
  • Author(s): 片岡宏誌, 松谷武嗣, T.Sakudoh, N.Yamanaka, 藤本善徳, 丹羽隆介
  • Organizer: International Conference on Cytochrome P450
  • Place of Presentation: Nago, Okinawa
• [Presentation] Control of developmental timing in Ecdysozoan: Studies on insect ecdysteroidogenesis and C.elegans heterochronic genes. (2008)
  • Author(s): 丹羽隆介, T.Yoshiyama, 片岡宏誌, F.Zhou, C.Li, F.J.Slack
  • Organizer: HHMI Janelia Farm Meeting : Molecular Mechanisms of Developmental timing
  • Place of Presentation: Verginia, USA
  • Year and Date: 2008-05-04
• [Presentation] Neverland, a conserved Rieske-type family of proteins essential for ecdysone synthesis and cholesterol metabolism in the prothoracic gland (2008)
  • Author(s): T.Yoshiyama, 丹羽隆介, 片岡宏誌
  • Organizer: 49th Drosophila Research Conference
  • Place of Presentation: San Diego, USA
• [Book] 内分泌と生命現象 昆虫の内分泌学(シリーズ21世紀の動物科学) (2007)
  • Author(s): 片岡宏誌
  • Publisher: 培風館
• [Book] シリーズ21世紀の動物科学「内分泌と生命現象」昆虫の内分泌学 (2007)
  • Author(s): 片岡宏誌
  • Total Pages: 21
  • Publisher: 培風館
• [Remarks] ホームページ等
  • URL: http://park.itc.u-tokyo.ac.jp/molecular-recognition/index.html