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System construction for the identification of parasitoid Hymenopter a with the combination of DNA barcodes and morphological images

Research Project

Project/Area Number 19380036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionKobe University

Principal Investigator

MAETO Kaoru  Kobe University, 大学院・農学研究科, 教授 (80346238)

Co-Investigator(Kenkyū-buntansha) MIURA Kazuki  独立行政法人農業・食品産業技術総合研究機構, 近畿中国四国農業研究センター・総合的害虫管理研究チーム, 特命チーム員 (10355133)
KONISHI Kazuhiko  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (90414747)
HAMAGUCHI Keiko  独立行政法人森林総合研究所, 関西支所, 主任研究員 (60343795)
Research Collaborator BELOKOBYLSKIJ Sergey A.  , ロシア科学アカデミー動物学研究所, 上席研究員
櫻井 厚志  神戸大学, 大学院・農学研究科, 研究員
東浦 祥光  , 山口県農林総合技術センター, 研究員
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywords天敵昆虫 / DNAバーコーディング / 寄生蜂 / 生物多様性情報 / DNAバーコード / 生物的防除 / COI / 分類 / 同定 / 天敵寄生蜂 / 分類・同定
Research Abstract

We have confirmed the validity of standard DNA barcoding region in the mitochondrial COI gene for species determination in parasitic wasps, developed protocols to extract DNA from a whole body of minute parasitic wasps without critical damages to external morphology as a voucher specimen, collected species records in Japan, designed the interface of supporting system for species identification, and thus established a technical base for DNA barcoding of parasitic wasps.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (62 results)

All 2011 2010 2009 2008 Other

All Journal Article (29 results) (of which Peer Reviewed: 19 results) Presentation (26 results) Book (6 results) Remarks (1 results)

  • [Journal Article] 昆虫類やダニ類のDNAクローニング法2011

    • Author(s)
      三浦一芸
    • Journal Title

      植物防疫 65巻

      Pages: 183-187

    • Related Report
      2010 Final Research Report
  • [Journal Article] Taxonomic status of Amebachia Uchida in the genus Netelia Gray (Hymenoptera; Ichneumonidae; Tryphoninae) with descriptions of four new species from Japan2010

    • Author(s)
      Konishi K
    • Journal Title

      Entomological Science Vol.13

      Pages: 216-225

    • NAID

      10027847038

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The first discovery of the genus Tossinola from Japan, with description of a new species (Hymenoptera, Ichneumonidae, Banchinae)2010

    • Author(s)
      Watanabe K, Ishikawa T, Konishi K
    • Journal Title

      Japanese Journal of Systematic Entomology Vol.16

      Pages: 25-31

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 昆虫類やダニ類のDNA2010

    • Author(s)
      三浦一芸
    • Journal Title

      シーケンス法と簡単な解析方法の紹介 64巻

      Pages: 766-773

    • Related Report
      2010 Final Research Report
  • [Journal Article] 昆虫類やダニ類からのDNA 抽出とPCR の実践2010

    • Author(s)
      三浦一芸
    • Journal Title

      植物防疫 64巻

      Pages: 620-625

    • Related Report
      2010 Final Research Report
  • [Journal Article] Taxonomic status of Amebachia Uchida in the genus Netelia Gray (Hymenoptera ; Ichneumonidae ; Tryphoninae) with descriptions of four new species from Japan2010

    • Author(s)
      小西和彦
    • Journal Title

      Entomological Science

      Volume: 13 Pages: 216-225

    • NAID

      10027847038

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first discovery of the genus Tossinola from Japan, with description of a new species (Hymenoptera, Ichneumonidae, Banchinae)2010

    • Author(s)
      渡辺恭平、小西和彦, 他
    • Journal Title

      Japanese Journal of Systematic Entomology

      Volume: 16 Pages: 25-31

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 昆虫類やダニ類からのDNA抽出とPCRの実践2010

    • Author(s)
      三浦一芸
    • Journal Title

      植物防疫

      Volume: 64 Pages: 620-625

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Parental genetic traits in offspring from inter-specific crosses between introduced and indigenous Diadegma Foerster (Hymenoptera : Ichneumonidae): possible effects on conservation genetics2009

    • Author(s)
      Davies PA, Takashino K, Watanabe M, Miura K
    • Journal Title

      Applied Entomology and Zoology Vol.44

      Pages: 535-541

    • NAID

      130000142900

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] マルチプレックスPCR法によるタバココナジラミバイオタイプの簡易識別法2009

    • Author(s)
      三浦一芸
    • Journal Title

      農業と園芸 84巻

      Pages: 1003-1010

    • NAID

      40016836219

    • Related Report
      2010 Final Research Report
  • [Journal Article] 種識別DNAマーカーの生態学・応用昆虫学への応用2009

    • Author(s)
      三浦一芸
    • Journal Title

      昆虫と自然 44巻

      Pages: 18-21

    • Related Report
      2010 Final Research Report
  • [Journal Article] Molecular biogeography of two sibling species of the sawfly genus Macrophya (Hymenoptera: Tenthredinidae) in Japan2009

    • Author(s)
      Sakurai A, Hamanishi Y, Maeto K, Naito C
    • Journal Title

      Zoological Science Vol.39

      Pages: 133-154

    • NAID

      40016725992

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular biogeography of two sibling species of the sawfly genus Macrophya (Hymenoptera : Tenthredinidae) in Japan2009

    • Author(s)
      Sakurai A, Hamanishi Y, Maeto K, Naito C
    • Journal Title

      Zoological Science 26

      Pages: 325-329

    • NAID

      40016725992

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] 特集・昆虫のDNAバーコーディング-昆虫の検索・同定と情報集積のための新しいツール2009

    • Author(s)
      前藤薫
    • Journal Title

      昆虫と自然 44(3)

      Pages: 2-3

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] 種識別DNAマーカーの生態学・応用昆虫学への応用2009

    • Author(s)
      三浦一芸
    • Journal Title

      昆虫と自然 44(3)

      Pages: 18-21

    • Related Report
      2009 Self-evaluation Report 2008 Annual Research Report
  • [Journal Article] Parental genetic traits in offspring from inter-specific crosses between introduced and indigenous Diadegma Foerster (Hymenoptera : Ichneumo-nidae) : possible effects on conservation genetics2009

    • Author(s)
      Davies PA, Takashino K, Watanabe M, Miura K
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 535-541

    • NAID

      130000142900

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] マルチプレックスPCR法によるタバココナジラミバイオタイプの簡易識別法2009

    • Author(s)
      三浦一芸
    • Journal Title

      農業と園芸 84

      Pages: 1003-1010

    • NAID

      40016836219

    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Journal Article] Parental genetic traits in offspring from inter-specific crosses between introduced and indigenous Diadegma Foerster(Hymenoptera : Ichneumonidae) : possible effects on conservation genetics2009

    • Author(s)
      Davies PA, Takashino K, Watanabe M, Miura K
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 535-541

    • NAID

      130000142900

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集・昆虫のDNAバーコーディングー昆虫の検索・同定と情報集積のための新しいツール2009

    • Author(s)
      前藤 薫
    • Journal Title

      昆虫と自然 44(3)

      Pages: 2-3

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Molecular biogeography of two sibling species of the sawfly genus Macrophya (Hymenoptera : Tenthredinidae) in Japan2009

    • Author(s)
      Sakurai, A., 前藤薫, 他
    • Journal Title

      Zoological Science (印刷中)

    • NAID

      40016725992

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asian Betylobraconinae (Hymenoptera, Braconidae), with description of a new genus and phylogenetic affinities of the tribe Facitorini2009

    • Author(s)
      Belokobylskij, S. A., 前藤薫, 他
    • Journal Title

      Insects Systematics and Evolution 39

      Pages: 133-154

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asian Betylobraconinae (Hymenoptera, Braconidae), with description of a new genus and phylogenetic affinities of the tribe Facitorini2008

    • Author(s)
      Belokobylskij SA, Zaldivar-Riveron A, Maeto K, Saez AG
    • Journal Title

      Insects Systematics and Evolution Vol.39

      Pages: 133-154

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Doryctinae (Hymenoptera, Braconidae) of Ogasawara Islands (Japan)2008

    • Author(s)
      Belokobylskij SA, Maeto K
    • Journal Title

      Annales Zoologici Vol.58

      Pages: 125-166

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multiplex method to discriminate between Neochrysocharis formosa (Hymenoptera : Eulophidae) reproductive modes2008

    • Author(s)
      Adachi-Hagimori T, Miura K
    • Journal Title

      Journal of Economic Entomology Vol.101

      Pages: 1510-1514

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Multiplex Method to Discriminate Between Neochrysocharis formosa (Hymenoptera : Eulophidae) Reproductive Modes2008

    • Author(s)
      Adachi-Hagimori T, Muira K
    • Journal Title

      Journal of Economic Entomology 101

      Pages: 1510-1514

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] (2008) Asian Betylobraconinae (Hymenoptera, Braconidae), with description of a new genus and phylogenetic affinities of the tribe Facitorini2008

    • Author(s)
      Belokobylskij SA, Zaldivar-Riveron EA, Maeto K, Saez AG
    • Journal Title

      Insects Systematics and Evolution 39

      Pages: 133-154

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Doryctinae (Hymenoptera, Braconidae) of Ogasawara Islands (Japan)2008

    • Author(s)
      Belokobylskij SA, Maeto K
    • Journal Title

      Annales Zoologici 58

      Pages: 125-166

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Doryctinae (Hymenoptera, Braconidae) of Ogasawara Islands (Japan)2008

    • Author(s)
      Belokobylskij, S. A., 前藤薫
    • Journal Title

      Annales Zoologici 58

      Pages: 125-166

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multiplex method to discriminate between Neochrysocharis formosa(Hymenoptera: Eulophidae)reproductive modes

    • Author(s)
      Adachi, Hagimori
    • Journal Title

      Journal of Economic Entomology (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 昆虫の同定にDNAって役に立つの?2011

    • Author(s)
      三浦一芸
    • Organizer
      第55回日本応用動物昆虫学会大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAバーコーディングによるハモグリバエ科害虫の寄生蜂同定支援システムの構築2010

    • Author(s)
      小西和彦、前藤薫
    • Organizer
      日本昆虫学会第70回大会
    • Place of Presentation
      山形大学(鶴岡市)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 北海道におけるアシグロハモグリバエの寄生蜂相2010

    • Author(s)
      小西和彦
    • Organizer
      日本昆虫学会第70回大会
    • Place of Presentation
      山形大学(鶴岡市)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAバーコーディングによるハモグリバエ科害虫の寄生蜂同定支援システムの構築2010

    • Author(s)
      小西和彦、前藤薫, 他
    • Organizer
      日本昆虫学会
    • Place of Presentation
      山形大学農学部(山形県)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 北海道におけるアシグロハモグリバエの寄生蜂相2010

    • Author(s)
      小西和彦
    • Organizer
      日本昆虫学会
    • Place of Presentation
      山形大学農学部(山形県)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子を利用すれば害虫と天敵の同定は簡単2010

    • Author(s)
      三浦一芸
    • Organizer
      日本昆虫学会第70回大会
    • Place of Presentation
      山形大学(鶴岡市)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 分子を利用すれば害虫と天敵の同定は簡単2010

    • Author(s)
      三浦一芸
    • Organizer
      日本昆虫学会
    • Place of Presentation
      山形大学農学部(山形県)(公開シンポジウム招待講演)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子生物学的手法を用いた圃場におけるタバコカスミカメの捕食歴調査2010

    • Author(s)
      伊藤政雄, 三浦一芸、渡部真也、渡辺衛介
    • Organizer
      日本応用動物昆虫学会第54回大会
    • Place of Presentation
      千葉大学(千葉県)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] 日本産昆虫類のDNAバーコーディング:寄生蜂への応用を中心に2010

    • Author(s)
      三浦一芸
    • Organizer
      第57回日本生態学会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-18
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 日本産昆虫類のDNAバーコーディング : 寄生蜂への応用を中心に2010

    • Author(s)
      三浦一芸, 前藤薫
    • Organizer
      第57回日本生態学会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-18
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Phenotypic plasticity and genetic variation of body color in uniparental strains of a parasitic wasp Meteorus pulchricornis (Hymenoptera, Braconidae)2010

    • Author(s)
      阿部陽介、前藤薫
    • Organizer
      Japan-Netherlands Seminar on Parasitoid Biology
    • Place of Presentation
      ポスター、東京大学(東京都)
    • Related Report
      2010 Final Research Report
  • [Presentation] Taxonomy of the genus Eurypterna with biological notes on E. cremieri (Ichneumonidae, Hybrizontinae).2010

    • Author(s)
      小西和彦、Konishi K
    • Organizer
      Seventh International Congress of Hymenopterists
    • Place of Presentation
      ポスター、Koszeg, Hungary
    • Related Report
      2010 Final Research Report
  • [Presentation] Taxonomy of the genus Eurypterna with biological notes on E.cremieri (Ichneumonidae, Hybrizontinae)2010

    • Author(s)
      小西和彦
    • Organizer
      Seventh International Congress of Hymenopterists
    • Place of Presentation
      Koszeg, Hungary(ポスター)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phenotypic plasticity and genetic variation of body color in uniparental strains of a parasitic wasp Meteorus pulchricornis (Hymenoptera, Braconidae)2010

    • Author(s)
      阿部陽介、前藤薫
    • Organizer
      Japan-Netherlands Seminar on Parasitoid Biology
    • Place of Presentation
      東京大学(東京都)(ポスター)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 天敵昆虫分類学の役割と今後の展望2009

    • Author(s)
      前藤薫
    • Organizer
      第53回日本応用動物昆虫学会大会シンポジウム
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2009-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] ギンケハラボソコマユバチの産雌性単為生殖のメカニズムと遺伝様式2009

    • Author(s)
      古江翔、前藤薫
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2009-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] 天敵昆虫分類学の役割と今後の展望2009

    • Author(s)
      前藤薫
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2009-03-30
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] ギンケハラボソコマユバチの産雌性単為生殖のメカニズムと遺伝様式2009

    • Author(s)
      古江翔, 前藤薫
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2009-03-30
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 天敵昆虫分類学の役割と今後の展望2009

    • Author(s)
      前藤 薫
    • Organizer
      第53回日本応用動物昆虫学会大会シンポジウム
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] ギンケハラボソコマユバチの産雌性単為生殖のメカニズムと遺伝様式2009

    • Author(s)
      古江翔
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNAバーコードの生態学・害虫管理への応用2008

    • Author(s)
      三浦一芸、前藤薫
    • Organizer
      日本昆虫学会第68回大会
    • Place of Presentation
      香川大学(高松市)
    • Year and Date
      2008-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAバーコードの生態学・害虫管理への応用2008

    • Author(s)
      三浦一芸, 前藤薫
    • Organizer
      日本昆虫学会第68回大会
    • Place of Presentation
      香川大学(香川県)
    • Year and Date
      2008-09-15
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] DNAバーコードの生態学・害虫管理への応用2008

    • Author(s)
      三浦一芸
    • Organizer
      日本昆虫学会第68回大会
    • Place of Presentation
      香川大学
    • Year and Date
      2008-09-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] 害虫防除におけるDNAバーコーディングの利用2008

    • Author(s)
      三浦一芸、前藤薫
    • Organizer
      第55回日本生態学会大会企画集会T04
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-03-14
    • Related Report
      2010 Final Research Report
  • [Presentation] 害虫防除におけるDNAバーコーディングの利用2008

    • Author(s)
      三浦一芸, 前藤薫
    • Organizer
      第55回日本生態学会大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2008-03-14
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 害虫防除におけるDNAバーコーディングの利用2008

    • Author(s)
      三浦一芸
    • Organizer
      第55回日本生態学会大会 企画集会T04
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-03-14
    • Related Report
      2007 Annual Research Report
  • [Book] ハモグリバエ科野菜・花卉害虫の寄生蜂群集、広渡編「絵かき虫の生物学」2011

    • Author(s)
      小西和彦
    • Publisher
      北隆館,東京(分担執筆)
    • Related Report
      2010 Annual Research Report
  • [Book] 絵かき虫の生物学(ハモグリバエ科野菜・花卉害虫の寄生蜂群集)(広渡編)2010

    • Author(s)
      小西和彦(分担執筆)
    • Publisher
      北隆館
    • Related Report
      2010 Final Research Report
  • [Book] Warshawska Drukarnia Naukowa, Doryctinae (Hymenoptera, Braconidae) of Japan2009

    • Author(s)
      Belokobylskij SA, Maeto K
    • Total Pages
      806
    • Related Report
      2010 Final Research Report
  • [Book] Doryctinae(Hymenoptera, Braconidae) of Japan2009

    • Author(s)
      Belokobylskij SA, Meato K
    • Total Pages
      806
    • Publisher
      Warshawska Drukarnia Naukowa
    • Related Report
      2009 Self-evaluation Report
  • [Book] Doryctinae(Hymenoptera, Braconidae)of Japan2009

    • Author(s)
      Belokobylskij, S.A.、Meato, K.
    • Total Pages
      806
    • Publisher
      Warshawska Drukarnia Naukowa, Warszawa
    • Related Report
      2009 Annual Research Report
  • [Book] 里山昆虫による生態系サービスを活かす. 「森林環境2009 生物多様性の日本」(森林環境研究会 編著)2009

    • Author(s)
      前藤薫(分担執筆)
    • Total Pages
      231
    • Publisher
      森林文化協会
    • Related Report
      2008 Annual Research Report
  • [Remarks] 新聞報道朝日新聞 2009年3月23日朝刊3面「DNAバーコード、進む登録食偽装や害虫調査に活用」に、前藤薫のインタビューを含む関連記事が掲載された。

    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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