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Interaction between Wolbachia exhibiting cytoplasmic incompatibility and its hosts

Research Project

Project/Area Number 19380038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

NODA Hiroaki  National Institute of Agrobiological Sciences, 昆虫・微生物間相互作用研究ユニット, ユニット長 (40343991)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords昆虫 / 共生微生物 / ウォルバキア / 細胞質不和合性 / マイクロアレイ
Research Abstract

Various aspects of intracellular symbionts, Wolbachia and Cardinium, were studied, with special reference to distribution among arthropods, cultivation in insect cell lines, gene expression profile of host cells, and classification of Cardinium. Cytoplasmic incompatibility was caused independently by Wolbachia and Cardinium in white-backed planthopper, Sogatella furcifera. The bacteria of the planthopper were successfully cultivated in insect cell lines. Immune response of host cells against bacteria was examined using microarrays. Caridinium induced immune response in the host cells whereas Wolbachia did not. Infection of Cardinium was surveyed in planthoppers, spider mites, and biting midges, which resulted in reclassification of the order Cardinium.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (54 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (33 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Differentially expressed genes in silkworm cell cultures in response to infection by Wolbachia and Cardinium endosymbionts.2011

    • Author(s)
      Nakamura Y, Gotoh T, Imanishi S, Mita K, Kurtti TJ, Noda H
    • Journal Title

      Insect Mol.Biol. (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differentially expressed genes in silkworm cell cultures in response to infection by Wolbachia and Cardinium endosymbionts.2011

    • Author(s)
      Nakamura Y, Gotoh T, Imanishi S, Mita K, Kurtti TJ, Noda H
    • Journal Title

      Insect Mol.Biol.

      Volume: 20(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transinfection of Wolbachia in Planthoppers : Nymphal injection of cultured Wolbachia and infection dynamics.2009

    • Author(s)
      Kawai S, Matsumoto Y, Gotoh T, Noda H
    • Journal Title

      Environ.Entomol. 38

      Pages: 1626-1633

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Cardinium in planthoppers and spider mites and taxonomic revision of "Candidatus Cardinium hertigii" based on detection of a new Cardinium group from biting midges.2009

    • Author(s)
      Nakamura Y, Kawai S, Yukuhiro F, Ito S, Gotoh T, Kisimoto R, Yanase T, Matsumoto Y, Kageyama D, Noda H
    • Journal Title

      Appl.Environ.Microbiol. 75

      Pages: 6757-6763

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Cardinium in planthoppers and spider mites and taxonomic revision of "Candidatus Cardinium hertigii" based on detection of a new Cardinium group from biting midges2009

    • Author(s)
      Nakamura Y, Kawai S, Yukuhiro F, Ito S, Gotoh T, Kisimoto R, Yanase T, Matsumoto Y, Kageyama D, Noda H
    • Journal Title

      Appl. Environ. Microbiol. 75

      Pages: 6757-6763

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Transinfection of Wolbachia in Planthoppers : Nymphal Injection of Cultured Wolbachia and Infection Dynamics2009

    • Author(s)
      Kawai S, Matsumoto Y, Gotoh T, Noda H
    • Journal Title

      Environ. Entomol. 38

      Pages: 1626-1633

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Transinfection of Wolbachia in planthoppers : Nymphal injection of cultured Wolbachia and infection dynamics.2009

    • Author(s)
      Kawai S, Matsumoto Y, Gotoh T, Noda H
    • Journal Title

      Environ.Entomol. 38

      Pages: 1626-1633

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens : a genomic resource for studying agricultural pests.2008

    • Author(s)
      Noda H, Kawai S, Koizumi Y, Matsui K, Zhang Q, Furukawa S, Shimomura M, Mita K
    • Journal Title

      BMC Genomics 9

      Pages: 117-117

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens : a genomic resource for studying agricultural pests2008

    • Author(s)
      Noda, H., Kawai, S., Koizumi, Y., Matsui, K., Zhang, Q., Furukawa, S., Shimomura, M., Mita, K.
    • Journal Title

      BMC Genomics 9

      Pages: 117-117

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Insecticidal effect of a fungicide containing polyoxin B on the larvae of Bombyx mori) Lepidoptera : Bombycidae), Mamestra brassicae, Mythimna separata, and Spodoptera litura (Lepidoptera : Noctuidae)2008

    • Author(s)
      Arakawa T, Yukuhiro F, Noda H.
    • Journal Title

      Appl. Entomol. Zool. 43

      Pages: 173-181

    • NAID

      110006684217

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial transfer of feminizing Wolbachia endosymbionts of the butterfly Eurema hecabe into the cells and intact body of the silkmoth Bombyx mori2008

    • Author(s)
      Kageyama D, Narita S, Noda H
    • Journal Title

      Microb. Ecol. 57

      Pages: 733-741

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 昆虫ゲノム研究の現状と今後の展開 : Arthropod Genomics Conference に出席して2008

    • Author(s)
      野田博明, 三田和英
    • Journal Title

      蚕糸・昆虫バイオテック 77

      Pages: 131-138

    • NAID

      10021921473

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens:a genomic resource for studying agricultural pests.2008

    • Author(s)
      Noda H, Kawai S, Koizumi Y, Matsui K, Zhang Q, Furukawa S, Shimomura M and Mita K
    • Journal Title

      BMC Genomics 9

      Pages: 117-117

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Y chromosomal PCR marker and production of a selected strain for molecular sexing in the brown planthopper,Nilaparvata lugens Stal.2007

    • Author(s)
      Kobayashi T and Noda H
    • Journal Title

      Arch.Insect Biochem.Physiol. 65

      Pages: 1-10

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wolbachia-induced cytoplasmic incompatibility in Japanese populations of Tetranychus urticae (Acari:Tetranychidae).2007

    • Author(s)
      Gotoh, T., Sugasawa, J., Noda, H. and Kitashima, Y
    • Journal Title

      Exp.Appl.Acarol. 42

      Pages: 1-16

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 共生細菌のゲノム研究:この10年の歩みと今後の展開2011

    • Author(s)
      野田博明
    • Organizer
      第55回日本応用動物昆虫学会
    • Place of Presentation
      九州大学
    • Related Report
      2010 Final Research Report
  • [Presentation] 昆虫共生菌の医薬・農薬探索資源としての利用法の開発2011

    • Author(s)
      小林秀昭・藤井理香・野田博明・竹石桂一
    • Organizer
      第55回日本応用動物昆虫学会
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒメトビウンカ終齢幼虫で起こる共生細菌スピロプラズマのオス殺し2011

    • Author(s)
      真田幸代・松村正哉・野田博明
    • Organizer
      第55回日本応用動物昆虫学会
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 共生細菌のゲノム研究:この10年の歩みと今後の展開2011

    • Author(s)
      野田博明
    • Organizer
      第55回日本応用動物昆虫学会
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞内共生細菌CardiniumおよびWolbachiaと宿主昆虫との相互作用2010

    • Author(s)
      中村有希・野田博明
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] ゲノム研究からみた応用動物昆虫学研究2010

    • Author(s)
      野田博明
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Sex ratio distortion by a Spiroplasma symbiont in the small brown planthopper, Laodelphax striatellus2010

    • Author(s)
      Sanada-Morimura S, Matsumura M, Noda H
    • Organizer
      13th International Auchenorrhyncha Congress
    • Place of Presentation
      Vaison-la-Romaine, France
    • Related Report
      2010 Annual Research Report
  • [Presentation] ハダニにおいて細胞質不和合性を誘導する共生微生物Cardinium2010

    • Author(s)
      吉岡主税・北嶋康樹・野田博明・後藤哲雄
    • Organizer
      第19回日本ダニ学会
    • Place of Presentation
      宮城教育大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] Male killing caused by a Spiroplasma symbiont in the small brown planthopper Laodelphax striatellus2010

    • Author(s)
      Sanada-Morimura S, Matsumura M, Noda H
    • Organizer
      Memorial Symposium for the 26th International Prize for Biology "Biology of Symbiosis"
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] Host cell immune responses against infection of Wolbachia, Cardinium and Rickettsia2010

    • Author(s)
      Nakamura Y, Watanabe K, Noda H
    • Organizer
      Memorial Symposium for the 26th International Prize for Biology "Biology of Symbiosis"
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] チャバネアオカメムシ共生菌ゲノム断片の大腸菌における発現の解析2010

    • Author(s)
      小林秀昭・野田博明・竹石桂一
    • Organizer
      第33回日本分子生物学会年会/第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Annual Research Report
  • [Presentation] 抗生物質処理がツマグロヨコバイの生育と共生微生物の感染に及ぼす影響2010

    • Author(s)
      河合佐和子・行弘文子・渡部賢司・野田博明
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒメトビウンカの細胞内共生細菌による性比偏向現象を無効化するオスの地理的分布2010

    • Author(s)
      真田幸代・松村正哉・野田博明
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒメトビウンカの細胞内共生細菌スピロプラズマの感染率と集団性比2009

    • Author(s)
      真田幸代, 松村正哉, 野田博明
    • Organizer
      第77回九州病害虫研究会
    • Place of Presentation
      熊本市
    • Year and Date
      2009-02-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞内共生細菌 Cardinium および Wolbachia と宿主昆虫との相互作用2009

    • Author(s)
      中村有希・野田博明
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] ヒメトビウンカの細胞内共生細菌による性比偏向現象を無効化するオスの発見2009

    • Author(s)
      真田幸代・松村正哉・野田博明
    • Organizer
      日本進化学会第11回大会
    • Place of Presentation
      北海道大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] キチョウからカイコへのメス化wolbachiaの感染移植.2008

    • Author(s)
      陰山 大輔・成田 聡子・野田 博明
    • Organizer
      第52回日本応用動物昆虫学会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細胞内共生細菌MolbachiaならびにCardiniumと宿主の免疫応答との関係.2008

    • Author(s)
      中村 有希・後藤 哲雄・T.J.Kurtti・今西 重雄・三田 和英・野田 博明
    • Organizer
      第52回日本応用動物昆虫学会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 殺菌剤として知られるポリオキシンの殺虫作用およびIGRとの協力作用.2008

    • Author(s)
      新川 徹・行弘 文子・野田 博明
    • Organizer
      第2回昆虫ゲノム研究会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] SNPマーカーを利用したカイコのBeauveria brongniartii菌抵抗性遺伝子の連関検索.2008

    • Author(s)
      和田 早苗・三国 辰男・渥美 省吾・生川 潤子・門野 敬子・山本 公子・三田 和英・宮本 和久・野田 博明
    • Organizer
      蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2008-03-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] トビイロウンカにおけるRNAi法の利用.2008

    • Author(s)
      河合 佐和子・野田 博明
    • Organizer
      第2回昆虫ゲノム研究会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-03-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] 昆虫細胞内共生細菌の感染により影響を受ける宿主細胞遺伝子のマイクロアレイ解析.2008

    • Author(s)
      中村 有希・三田 和英・野田 博明
    • Organizer
      第2回昆虫ゲノム研究会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-03-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細胞内共生細菌WolbachiaならびにCardiniumと宿主の免疫応答との関係2008

    • Author(s)
      中村有希・後藤哲雄・T.J.Kurtti・今西重雄・三田和英・野田博明
    • Organizer
      第52回日本応用動物昆虫学会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ黄萎病媒介虫ツマグロヨコバイにおけるファイトプラズマ感染の定量2008

    • Author(s)
      渡部賢司, 佐伯知明, 野田博明
    • Organizer
      平成20年度日本植物病理学会
    • Place of Presentation
      松江市
    • Related Report
      2008 Annual Research Report
  • [Presentation] タバコシバンムシの盲嚢と共生微生物2008

    • Author(s)
      行弘文子, 野田博明
    • Organizer
      医学生物学電子顕微鏡技術学会
    • Place of Presentation
      神奈川歯科大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞内共生細菌スピロプラズマによるヒメトビウンカの性比偏向2008

    • Author(s)
      真田幸代, 松村正哉, 野田博明
    • Organizer
      日本進化学会第10回大会
    • Place of Presentation
      東大駒場
    • Related Report
      2008 Annual Research Report
  • [Presentation] Planthopper genomics:molecular approaches to planthopper problems.2007

    • Author(s)
      Noda H
    • Organizer
      International Workshop on Forecasting and Management of Rice Planthoppers in East Asia:Their Ecology and Genetics
    • Place of Presentation
      熊本市
    • Year and Date
      2007-12-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Gene expression correlated to the phase change of the brown planthopper.2007

    • Author(s)
      Kobayashi T, Noda H
    • Organizer
      International Workshop on Forecasting and Management of Rice Planthoppers in East Asia:Their Ecology and Genetics
    • Place of Presentation
      熊本市
    • Year and Date
      2007-12-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] 昆虫病原糸状菌Beauveria brongniartii感受性および抵抗性カイコ幼虫の血球で発現する遺伝子の比較.2007

    • Author(s)
      和田 早苗・中村 有希・渥美 省吾・村上 理都子・三橋 渡・宮本 和久・野田 博明
    • Organizer
      蚕糸学会関東支部会
    • Place of Presentation
      東京大学
    • Year and Date
      2007-11-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] Bacillus thuringiensis産生毒素Cry1Abに対するカイコの抵抗性遺伝子のマップベースドクローニング法による解析.2007

    • Author(s)
      渥美 省吾・宮本 和久・山本 公子・門野 敬子・三田 和英・和田 早苗・小瀬川 英一・神田 康三・野田 博明
    • Organizer
      第13回BT研究会
    • Place of Presentation
      新潟県西蒲原郡弥彦村
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Linkage and mapping analysis of a gene resistant to Bacillus thuringiensis Cry1Ab toxin in the silkworm Bombyx mori.2007

    • Author(s)
      Atsumi S, Miyamoto K, Yamamoto K, Narukawa J, Mita K, Kadono-Okuda K, Wada S, Kosegawa E, Kanda K, Golds mith MR, and Noda H
    • Organizer
      Annual Meeting of Society for Invertebrate Pathology
    • Place of Presentation
      ケベック、カナダ
    • Year and Date
      2007-08-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] シバンムシの共生微生物を観察するための固定法の検討.2007

    • Author(s)
      行弘 文子・野田 博明
    • Organizer
      医学生物学電子顕微鏡技術学会
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2007-05-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Bacillus thuringiensis産生毒素Cry1Abに対するカイコの抵抗性遺伝子座位情報の高度化.2007

    • Author(s)
      渥美 省吾・宮本 和久・山本 公子・生川 潤子・三田 和英・和田 早苗・小瀬川 英一・神田 康三・原和 二郎・野田 博明
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      農林水産筑波事務所
    • Year and Date
      2007-04-03
    • Related Report
      2007 Annual Research Report
  • [Book] IRRI, Los Banos (Philippines)(K.L.Heong and B.Hardy eds.)2009

    • Author(s)
      野田博明
    • Publisher
      How planthopper genomics can be useful for planthopper management? In "Planthoppers-new threats to the sustainability of intensive rice production systems in Asia"
    • Related Report
      2010 Final Research Report
  • [Book] 微生物の事典(昆虫と微生物)(分担執筆)2008

    • Author(s)
      野田博明, 他共同執筆
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 昆虫と微生物.「微生物の事典」2008

    • Author(s)
      野田博明, 他
    • Publisher
      朝倉書店
    • Related Report
      2009 Self-evaluation Report
  • [Book] 微生物の事典2008

    • Author(s)
      野田博明, 分担執筆
    • Total Pages
      732
    • Publisher
      朝倉書店
    • Related Report
      2008 Annual Research Report
  • [Book] 農薬用語辞典2008

    • Author(s)
      野田博明, 共同執筆
    • Total Pages
      405
    • Publisher
      日本植物防疫協会
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.nias.affrc.go.jp/renkei_daigakuin2/i_index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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