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Molecular mechanism of phosphate transport in plant-fungal symbiosis

Research Project

Project/Area Number 19380040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionHokkaido University

Principal Investigator

EZAWA Tatsuhiro  Hokkaido University, 大学院・農学研究院, 准教授 (40273213)

Co-Investigator(Kenkyū-buntansha) AONO Toshihiro  東京大学, 生物生産工学研究センター, 助教 (10372418)
Co-Investigator(Renkei-kenkyūsha) YUKARI Kuga  信州大学, 農学部, 准教授 (30232747)
Research Collaborator TANI Chiharu  北海道大学, 大学院・農学研究院, 研究員
HIJIKATA Nowaki  北海道大学, 大学院・農学院, 博士課程
MURASE Masatake  北海道大学, 大学院・農学院, 修士課程
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsアーバスキュラー菌根菌 / 共生 / リン酸 / オルガネラ / ポリリン酸
Research Abstract

The present study demonstrated that i) polyphosphate is the main storage of phosphorus in arbuscular mycorrhizal fungi, ii) polyphosphate consists of 60-70% of total cellular phosphorus at maximum, and iii) Na^+, K^+, Ca^<2+>, and Mg^<2+> are also accumulated during polyphosphate accumulation to neutralize negative charge of polyphosphate. An efficient method to extract RNA from hyphae has been established for large scale sequencing of mRNA expressed during polyphosphate accumulation in hyphae.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (37 results)

All 2010 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (27 results) Remarks (1 results)

  • [Journal Article] Polyphosphate has a central role in the rapid and massive accumulation of phosphorus in extraradical mycelium of an arbuscular mycorrhizal fungus.2010

    • Author(s)
      Hijikata N., Murase M., Tani C., Ohtomo R., Osaki M., Ezawa T.
    • Journal Title

      New Phytologist 186

      Pages: 285-289

    • NAID

      120003860265

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyphosphate has a central role in the rapid and massive accumulation of phosphorus in extraradical mycelium of an arbuscular mycorrhizal fungus2010

    • Author(s)
      Hijikata, et al.
    • Journal Title

      New Phytologist

      Volume: 186 Pages: 285-289

    • NAID

      120003860265

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyphosphate-synthesizing activity in extraradical hyphae of an arbuscular mycorrhizal fungus : ATP-dependent but proton gradient-independent synthesis.2009

    • Author(s)
      Tani C., Ohtomo R., Osaki M., Kuga Y., Ezawa T.
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 7044-7050

    • URL

      http://hdl.handle.net/2115/43031

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyphosphate-synthesizing activity in extraradical hyphae of an arbuscular mycorrhizal fungus : ATP-dependent but proton gradient-independent synthesis2009

    • Author(s)
      Tani, et al.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 75 Pages: 7044-7050

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyphosphate kinase is essential for swarming motility, tolerance to environmental stresses, and virulence in Pseudomonas syringae pv.tabaci 6605.2008

    • Author(s)
      Hossain M.M., Tani C., Suzuki T., Taguchi F., Ezawa T., Ichinose Y.
    • Journal Title

      Physiological and Molecular Plant Pathology 72

      Pages: 122-127

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant symbiotic microorganisms in acid sulfate soil : significance in the growth of pioneer2008

    • Author(s)
      Maki, et.al.
    • Journal Title

      Plant and Soil 310

      Pages: 55-65

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Community structure of arbuscular mycorrhizal fungi associated with pioneer grass species Miscanthus sinensis in acid sulfate soils : habitat segregation along pH gradients2008

    • Author(s)
      An, et.al.
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 517-528

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant symbiotic microorganisms in acid sulfate soil : significance in the growth of pioneer plants2008

    • Author(s)
      Maki, et al.
    • Journal Title

      Plant and Soil 310

      Pages: 55-65

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Community structures of arbuscular mycorrhizal fungi associated with pioneer grass species Miscanthus sinensis in acid sulfate soils : habitat segregation along pH gradients2008

    • Author(s)
      An, et al.
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 517-528

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] アーバスキュラー菌根菌外生菌糸におけるポリリン酸超集積時のトランスクリプトーム:リン酸吸収・濃縮・輸送に関わる遺伝子群の発現ネットワーク2010

    • Author(s)
      菊池裕介, ほか
    • Organizer
      菌根研究会
    • Place of Presentation
      千葉大(松戸)
    • Year and Date
      2010-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Plant transpiration is a possible driving force for phosphate translocation in the extraradical hyphae of an arbuscular mycorrhizal fungus2010

    • Author(s)
      Kikuchi, et al.
    • Organizer
      1^<st> Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Miyazaki, Japan
    • Year and Date
      2010-09-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌における宿主方向へのリン酸輸送ポリリン酸輸送の駆動力としての蒸散2010

    • Author(s)
      菊池裕介, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      北海道大(札幌)
    • Year and Date
      2010-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるポリリン酸代謝:潜在的蓄積能とその電荷バランス調節メカニズム2009

    • Author(s)
      土方野分, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都
    • Year and Date
      2009-09-16
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるポリリン酸代謝:潜在的蓄積能とその電荷バランス調節メカニズム2009

    • Author(s)
      土方野分, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大(京都)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌の外生菌糸による酸性ホスファターゼの分泌2009

    • Author(s)
      松田健一郎, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大(京都)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌感染に応答するミヤコグサ酸性ホスファターゼ遺伝子の探索2009

    • Author(s)
      西村あおい, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大(京都)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dynamics of inorganic and organic cations during polyphosphate-hyperaccumulation in an arbuscular mycorrhizal fungus2009

    • Author(s)
      Hijikata, et al.
    • Organizer
      6^<th> International Conference on Mycorrhiza
    • Place of Presentation
      Belo Horizonte, Brazil
    • Year and Date
      2009-08-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Polyphosphate is responsible for the rapid and massive accumulation of phosphorus in arbuscular mycorrhizal fungi2009

    • Author(s)
      Ezawa, et al.
    • Organizer
      6^<th> International Conference on Mycorrhiza
    • Place of Presentation
      Belo Horizonte, Brazil
    • Year and Date
      2009-08-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dynamics of inorganic and organic cations during polyphosphate-hyperaccumulation in an arbuscular mycorrhizal fungus.2009

    • Author(s)
      Hijikata, et al.
    • Organizer
      6th International Conference on Mycorrhiza
    • Place of Presentation
      Belo Horizonte, Brazil
    • Related Report
      2009 Final Research Report
  • [Presentation] Polyphosphate is responsible for the rapid and massive accumulation of phosphorus in arbuscular mycorrhizal fungi.2009

    • Author(s)
      Ezawa, et al.
    • Organizer
      6th International Conference on Mycorrhiza
    • Place of Presentation
      Belo Horizonte, Brazil
    • Related Report
      2009 Final Research Report
  • [Presentation] 植物のリン酸獲得戦略としての菌根形成-リン酸濃縮と長距離輸送メカニズムへのアプローチ-2008

    • Author(s)
      江沢辰広
    • Organizer
      東京大学生物生産工学センターシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2008-12-05
    • Related Report
      2009 Final Research Report
  • [Presentation] 植物のリン酸獲得戦略としての菌根形成ーリン酸濃縮と長距離輸送メカニズムへのアプロー2008

    • Author(s)
      江沢辰広
    • Organizer
      東京大学生物生産工学研究センターシンポジウム「土壌-植物系の物質移行 : 生産性向上を目指して」
    • Place of Presentation
      東京
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積:ポリリン酸蓄積と同調的に吸収されるカチオンの特定2008

    • Author(s)
      土方野分, ほか
    • Organizer
      菌根研究会
    • Place of Presentation
      鶴岡
    • Year and Date
      2008-10-18
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積 : ポリリン酸蓄積と同調的に吸収されるカチ2008

    • Author(s)
      土方野分ほか
    • Organizer
      2008年度菌根研究会大会
    • Place of Presentation
      鶴岡
    • Year and Date
      2008-10-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] 共生菌からのリン酸移行の抑制がアーバスキュラー菌根に拳ける宿主/菌遺伝子群の発現2008

    • Author(s)
      青野俊裕・江澤辰広
    • Organizer
      日本土壌肥料学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積:ポリリン酸合成システム2008

    • Author(s)
      谷千春, ほか
    • Organizer
      農芸化学会北海道支部会
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-13
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積:ポリリン酸の潜在的蓄積能とその対イオンとの関係2008

    • Author(s)
      土方野分, ほか
    • Organizer
      農芸化学会北海道支部会
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-13
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積 : ポリリン酸合成システム2008

    • Author(s)
      谷千春ほか
    • Organizer
      日本農芸化学会札幌支部会
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン酸超集積 : ポリリン酸の潜在的蓄積能とその対イオン2008

    • Author(s)
      土方野分ほか
    • Organizer
      日本農芸化学会札幌支部会
    • Place of Presentation
      札幌
    • Year and Date
      2008-08-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン輸送のプロファイリング:オルトリン酸の恒常性と全リンおよびポリリン酸の同調的変化2007

    • Author(s)
      土方野分, ほか
    • Place of Presentation
      津
    • Year and Date
      2007-11-17
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるリン輸送のプロファイリング:オルトリン酸の恒常性と全リンおよびポリリン酸の同調的変化2007

    • Author(s)
      土方野分, ほか
    • Organizer
      菌根研究会
    • Place of Presentation
      津市・三重大学
    • Year and Date
      2007-11-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるポリリン酸合成および輸送ポテンシャル2007

    • Author(s)
      村瀬正剛, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      秋田
    • Year and Date
      2007-08-24
    • Related Report
      2009 Final Research Report
  • [Presentation] アーバスキュラー菌根菌におけるATP依存性ポリリン酸合成活性-リン酸による活性誘導とその酵素学的特性-2007

    • Author(s)
      谷千春, ほか
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島
    • Related Report
      2009 Final Research Report
  • [Presentation] 凍結置換法によるアーバスキュラー菌根菌の液胞内ポリリン酸の観察2007

    • Author(s)
      名雪桂一郎, ほか
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      柏市・東葛テクノプラザ
    • Related Report
      2007 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌におけるポリリン酸合成および輸送ポテンシャル2007

    • Author(s)
      谷千春, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京・東京農業大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌および酵母におけるアルカリフォスファターゼのポリリン酸代謝への関与2007

    • Author(s)
      木澤恵子, ほか
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京・東京農業大学
    • Related Report
      2007 Annual Research Report
  • [Remarks] 成果掲載ホームページ

    • URL

      http://www.agr.hokudai.ac.jp/botagr/rhizo/RhizoCont/index.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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