| Project/Area Number |
19380041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SENOO Keishi The University of Tokyo, 大学院・農学生命科学研究科, 教授 (40206652)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUKA Shigeto 東京大学, 大学院・農学生命科学研究科, 准教授 (10313074)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 水田土壌 / 脱窒細菌 / 土壌核酸 / SIP解析 / FSC分離法 / クローンライブラリ解析 / 脱窒機能遺伝子 / 16S rRNA遺伝子 / 土壌DNA / 土壌RNA / 定量PCR / 土壌微生物 / nirS / nirK / 16S rRNA / 群集構造解析 / 亜硝酸還元酵素遺伝子 / Stable Isotope Probins法 |
|---|
| Research Abstract |
Rice paddy soil has been shown to have strong denitrifying activity. However, the microbial populations responsible for denitrification have not been well characterized. We used multiple approaches, including stable isotope probing, large-scale 16S rRNA gene sequence analysis, nitrite reductase gene analysis, and a novel single-cell isolation procedure, to identify the key players in denitrification in rice paddy soil. Based on the culture-independent analyses, bacteria in the orders Burkholderiales and Rhodocyclales were identified as the key players in denitrification in rice paddy soil under both laboratory and field conditions. Based on 16S rRNA gene, nirS and nirK sequence, oligonucleotide probes for phylogenetic and functional microarray were successfully designed.
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