| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Research Abstract |
This study investigated the adsorptions of DNA molecules by real soils and variable-charged soil constituents such as goethite, allophane, and humic acids for understanding risk assessment for release of the genetically modified organisms and for development of DNA extraction efficiency from soils in the analysis of soil microbial communities using culture-independent methods. The DNA adsorption on an andosol used in this study was much larger than those on a fluvisol and an acrisol. The changes in DNA adsorptions by the decrease in organic matter and oxide minerals implied that these oxide minerals among soil constituents are one of the main media to adsorb DNA molecules in soil environments. The adsorptions of DNA by andosols and allophane were investigated as function of solution pH, ionic strength in solution and some solutes. These results suggested that there are several DNA adsorption mechanisms in soil.
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