Analysis of novel cell surface proteins affecting cell shape of bacteria

• Project/Area Number: 19380047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: SEKIGUCHI Junichi Shinshu University, 大学院・総合工学系研究科, 教授 (80111053)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Hiroki 信州大学, 繊維学部, 准教授 (20262701) ; HASHIMOTO Masayuki 信州大学, ファイバーナノテク国際若手研究者育成拠点, 助教 (80402139) ; FUKUSHIMA Tatsuya 信州大学, ヒト環境科学研究支援センター, 助教 (20529200)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
• Keywords: 微生物機能 / 表層蛋白質 / 細胞形態 / 細胞壁溶解酵素 / インヒビター / 枯草菌 / 応用微生物 / ゲノム / 細菌 / 蛋白質 / 発現制御 / 細胞壁 / 細胞表層 / 形態形成 / 阻害剤 / 細胞分離 / ペプチドグリカン

Research Abstract

Bacillus subtilis gene products (IseA and YqgA) associated with cell shape were investigated extensively. IseA is a proteinaceous inhibitor against the DL-endopeptidase family and its overexpression led to typical cell filamentation. It is likely that YqgA had some relation with cell wall lytic DL-endopeptidases (LytE, LytF and CwlS). The role of LytE, which is one of the synthetic lethal proteins, is investigated in detail. Moreover, enzymatic properties of B. subtilis CwlK and CwlT are reported.

Research Products

• [Journal Article] 枯草菌における細胞の分離 (2009)
  • Author(s): 橋本昌征, 山本博規, 関口順一
  • Journal Title: 繊維と工業 65(8) Pages: 282-286
  • NAID: 10025317706
• [Journal Article] The major and minor wall teichoic acids prevent the sidewall localization of vegetative D,L-endopeptidase LytF in Bacillus subtilis . (2008)
  • Author(s): Yamamoto H., Y.Miyake, M.Hisaoka, S.Kurosawa, J.Sekiguchi
  • Journal Title: Mol.Microbiol. 70(2) Pages: 297-310
  • Peer Reviewed
• [Journal Article] Identification and characterization of new cell wall hydrolase, CwlT: a two-domain hydrolase of N -acetylmuramidase and D,L-endopeptidase. (2008)
  • Author(s): Fukushima T., T.Kitajima, H.Yamaguchi, Q.Ouyang, K.Furuhata, H.Yamamoto, T.Shida, J.Sekiguchi
  • Journal Title: J.Biol.Chem. 283(17) Pages: 11117-11125
  • Peer Reviewed
• [Journal Article] Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis . (2008)
  • Author(s): Yamamoto H., M.Hashimoto Y.Higashitsuji, H.Harada, N.Hariyama, L.Takahashi, T.Iwashita, S.Ooiwa, J.Sekiguchi
  • Journal Title: Mol.Microbiol. 70(1) Pages: 168-182
  • Peer Reviewed
• [Journal Article] Identification and characterization of new cell wall hydrolase, CwlT : a two-domain hydrolase of N-acetylmuramidase and D, L-endopeptidase (2008)
  • Author(s): Fukushima, T., T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, J. Sekiguchi
  • Journal Title: J.Biol.Chem. 283(17) Pages: 11117-11125
  • Peer Reviewed
• [Journal Article] Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis (2008)
  • Author(s): Yamamoto, H., M. Hashimoto, Y. Higashitsuji, H. Harada, N. Hariyama, L. Takahashi, T. Iwashita, S. Ooiwa, J. Sekiguchi
  • Journal Title: Mol.Microbiol. 70(1) Pages: 168-182
  • Peer Reviewed
• [Journal Article] The major and minor wall teichoic acids prevent the sidewall localization of vegetative D, L-endopeptidase LytF in Bacillus subtilis (2008)
  • Author(s): Yamamoto, H., Y. Miyake, M. Hisaoka, S. Kurosawa, J. Sekiguchi
  • Journal Title: Mol.Microbiol. 70(2) Pages: 297-310
  • Peer Reviewed
• [Journal Article] Identification and characterization of new cell wall hydrolase, CwlT : a two-domain hydrolase of N-acety lmuramidase and D, L-endopcptidase (2008)
  • Author(s): Fukushima, T., T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, J. Sekiguchi
  • Journal Title: J. Biol. Chem. 283(17) Pages: 11117-11125
  • Peer Reviewed
• [Journal Article] Post-translalional control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis (2008)
  • Author(s): Yamamoto, H., M. Hashimoto, Y. Higashitsuji, H. Harada, N. Hariyama, L. Takahashi, T. Iwashita, S. Ooiwa, J. Sekiguchi
  • Journal Title: Mol. Microbiol. 70(1) Pages: 168-182
  • Peer Reviewed
• [Journal Article] The major and minor wall teichoic acids prevent the sidewall localization of vegelative D, L-endopcptidase LytF in Bacillus subtilis. (2008)
  • Author(s): Yamanioto, h., Y. Miyake, M. Hisaoka, S. Kurosawa, J. Sukiguchi
  • Journal Title: Mol. Microbiol. 70(2) Pages: 297-310
  • Peer Reviewed
• [Journal Article] Characterization of a new L,D-endopeptidase gene product CwlK (previous YcdD) hydrolyzing peptidoglycan in Bacillus subtilis . (2007)
  • Author(s): Fukushima T., Y.Yao, T.Kitajima, H.Yamamoto, J.Sekiguchi.
  • Journal Title: Mol.Genet.Genomics 278 Pages: 371-383
  • Peer Reviewed
• [Journal Article] Characterization of a new L, D-endopeptidase gene product Cw1K(previous YcdD)hydrolyzing peptidoglycan in Bacillus subtilis (2007)
  • Author(s): Fukushima, T., Y. Yao, T. Kitajima, H. Yamamoto, and J. Sekiguchi
  • Journal Title: Mol. Genet. Genomics 278 Pages: 371-383
  • Peer Reviewed
• [Presentation] Bacillus subtilis CwlP in the SP-beta prophage comprises two novel cell wall hydrolase domains (2009)
  • Author(s): I Putu Sudiarta, T.Fukushima, J.Sekiguchi
  • Organizer: BioMicroWorld
  • Place of Presentation: リスボン大学(リスボン)
  • Year and Date: 2009-12-02
• [Presentation] Bacillus subtilis CwlP in the SP-beta prophage comprises two novel cell wall hydrolase domains (2009)
  • Author(s): I Putu Sudiarta, T. Fukushima, J. Sekiguchi
  • Organizer: BioMicroWorld 2009
  • Place of Presentation: リスボン大学 (リスボン)
  • Year and Date: 2009-12-02
• [Presentation] Bacillus subtilis CwlP in the SP-beta prophage comprises two novel cell wall hydrolase domains (2009)
  • Author(s): I Putu Sudiarta, T.Fukushima, J.Sekiguchi
  • Organizer: BioMicroWorld 2009
  • Place of Presentation: リスボン大学(リスボン)
  • Year and Date: 2009-12-02
• [Presentation] ゲノムで紐解く細胞壁結合蛋白質群の機能 (2009)
  • Author(s): 関口順一, 福島達也, 橋本昌征, 山本博規
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 信州大学(松本市)
  • Year and Date: 2009-09-16
• [Presentation] ゲノムで紐解く細胞壁結合蛋白質群の機能 (2009)
  • Author(s): 関口順一, 福島達也, 橋本昌征, 山本博規
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 信州大学 (松本市)
  • Year and Date: 2009-09-16
• [Presentation] PdaC (YjeA) deacetylates the acetyl groups of N -acetylglucosamine in the chitin oligomers and N-acetylmuramic acid in peptidoglycan -Biochemical approach for identification of PdaC in Bacillus subtilis- 5th Internatl. (2009)
  • Author(s): K.Kobayashi, T.Kodama, T.Fukushima, K.Ara, K.Ozaki, J.Sekiguchi
  • Organizer: Conf.On Gram-Positive Microorganisms
  • Place of Presentation: Catamaran ホテ(サンディエゴ)
  • Year and Date: 2009-06-15
• [Presentation] PdaC (YjeA) deacetylates the acetyl groups of N-acetylglucosamine in the chitin oligomers and N-acetylmuramic acid in peptidoglycan-Biochemical approach for identification of PdaC in Bacillus subtilis- (2009)
  • Author(s): K. Kobayashi, T. Kodama, T. Fukushima, K. Ara, K. Ozaki, J. Sekiguchi
  • Organizer: 5^<th> Internatl.Conf.on Gram-Positive Microorganisms
  • Place of Presentation: Catamaran ホテル (サンディエゴ)
  • Year and Date: 2009-06-15
• [Presentation] PdaC (YjeA) deacetylates the acetyl groups of N-acetylglucosamine in the chitin oligomers and N-acetylmuramic acid in peptidoglycan-Biochemical approach for identification of PdaC in Bacillus subtilis- (2009)
  • Author(s): K.Kobayashi, T.Kodama, T.Fukushima, K.Ara, K.Ozaki, J.Sekiguchi
  • Organizer: 5^<th> Internatl. Conf. on Gram-Positive Microorganisms
  • Place of Presentation: Catamaranホテル(サンディエゴ)
  • Year and Date: 2009-06-15
• [Presentation] Peptidoglycan degradation and modification enzymes are important in basic and applied research on microbiology. (2008)
  • Author(s): 関口順一
  • Organizer: iBio-2008
  • Place of Presentation: Hangzhou, Hangzhou,Session 6, Part 1.
• [Presentation] Proteinaceous inhibitor affecting activity of cell separation enzymes in Bacillus subtilis . (2007)
  • Author(s): Sekiguchi J., T.Iwashita, H.Yamamoto.
  • Organizer: 4th Conference on Functional Genomics of Gram-Positive Microorganisms
  • Place of Presentation: Tirrenia
• [Presentation] Proteinaceous inhibitor affecting activity of cell separation enzymes in Bacillus subtilis (2007)
  • Author(s): Junichi Sekiguchi, Tomoaki Iwashita, and Hiroki Yamamoto
  • Organizer: 4th Internatl. Conf. on Functional Genomics of Gram-positive microorganisms
  • Place of Presentation: Tirrenia, Italy
• [Book] 微生物機能を活用した革新的生産技術の最前線(清水晶他編)(枯草菌のミニマムゲノムファクトリー) (2007)
  • Author(s): 荒勝俊, 尾崎克也, 掛下大視, 中村幸治, 山根國男, 児玉武子, 関口順一, 門屋享介, 森本拓也, 小笠原直毅
  • Total Pages: 201
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] p60エンドペプチダーゼ阻害剤 (2008)
  • Inventor(s): 荒勝俊、阪本知生、山本博規、針山望、関口順一
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 2008-078828
  • Filing Date: 2008-03-25
• [Patent(Industrial Property Rights)] p60エンドペプチダーゼ阻害剤 (2008)
  • Inventor(s): 荒勝俊, 阪本知生, 山本博規, 針山望, 関口順一
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 2008-078828
  • Filing Date: 2008-03-25
• [Patent(Industrial Property Rights)] 溶菌酵素阻害剤、溶菌抑制剤及びポリ-ガンマ-グルタミン酸の分解抑制剤及びポリ-ガンマ-グルタミン酸の製造方法 (2007)
  • Inventor(s): 荒勝俊、関口順一、山本博規、原田宏之
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 2007-268523
  • Filing Date: 2007-10-16
  • Overseas
• [Patent(Industrial Property Rights)] 溶菌酵素阻害剤、溶菌抑制剤及びポリーガンマーグルタミン酸の分解抑制剤及びポリーガンマーグルタミン酸の製造方法 (2007)
  • Inventor(s): 荒勝俊, 関口順一, 山本博規, 原田宏之
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 2007-268523
  • Filing Date: 2007-10-16