Analysis of novel cell surface proteins affecting cell shape of bacteria
| Project/Area Number |
19380047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shinshu University |
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Principal Investigator |
SEKIGUCHI Junichi Shinshu University, 大学院・総合工学系研究科, 教授 (80111053)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hiroki 信州大学, 繊維学部, 准教授 (20262701)
HASHIMOTO Masayuki 信州大学, ファイバーナノテク国際若手研究者育成拠点, 助教 (80402139)
FUKUSHIMA Tatsuya 信州大学, ヒト環境科学研究支援センター, 助教 (20529200)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | 微生物機能 / 表層蛋白質 / 細胞形態 / 細胞壁溶解酵素 / インヒビター / 枯草菌 / 応用微生物 / ゲノム / 細菌 / 蛋白質 / 発現制御 / 細胞壁 / 細胞表層 / 形態形成 / 阻害剤 / 細胞分離 / ペプチドグリカン |
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| Research Abstract |
Bacillus subtilis gene products (IseA and YqgA) associated with cell shape were investigated extensively. IseA is a proteinaceous inhibitor against the DL-endopeptidase family and its overexpression led to typical cell filamentation. It is likely that YqgA had some relation with cell wall lytic DL-endopeptidases (LytE, LytF and CwlS). The role of LytE, which is one of the synthetic lethal proteins, is investigated in detail. Moreover, enzymatic properties of B. subtilis CwlK and CwlT are reported.
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Report
(5 results)
Research Products
(28 results)
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[Journal Article] Identification and characterization of new cell wall hydrolase, CwlT : a two-domain hydrolase of N-acetylmuramidase and D, L-endopeptidase2008
Author(s)
Fukushima, T., T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, J. Sekiguchi
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Journal Title
J.Biol.Chem. 283(17)
Pages: 11117-11125
Related Report
Peer Reviewed
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[Journal Article] Identification and characterization of new cell wall hydrolase, CwlT : a two-domain hydrolase of N-acety lmuramidase and D, L-endopcptidase2008
Author(s)
Fukushima, T., T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, J. Sekiguchi
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Journal Title
J. Biol. Chem. 283(17)
Pages: 11117-11125
Related Report
Peer Reviewed
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