Molecular analysis of the earliest step of ecological interaction of environmental bacteria

• Project/Area Number: 19380049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hiroshima University
• Principal Investigator: KATO Junichi Hiroshima University, 大学院・先端物質科学研究科, 教授 (90231258)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 生態学的振舞い / 走化性 / 生物相互作用 / バイオレメディエーション / センサー / 植物-微生物相互作用 / トリクロロエチレン / 走化性トランスデューサー / Pseudomonas fluorescens / Pseudomonas putida / 環境修復 / 物質感知機能 / 走化性センサー / 環境細菌 / 生態学的振舞 / 運動性細菌 / Pseudomonas

Research Abstract

We found that Pseudomonas strains show chemotactic responses to plant-related compounds including amino acids, malate, and ethylene. We identified P.aeruginosa PA26541, P.putida Pput_3489, and P.aeruginosa PA2652 as chmotactic sensory proteins for ethylene, amino acids, and malate, respectively. Molecular analysis of chemotaxis to trichloroethylene (TCE) in TCE-degrading bacterium P.putida F1 revealed that Pput_6181 and Pput_3489 serve as sensory proteins for positive and negative chemotaxis to TCE, respectively.

Research Products

• [Journal Article] Pseudomonas as a model microorganism for investigation of chemotactic behaviors in ecosystem. (2008)
  • Author(s): J. Kato, H.-E. Kim, N. Takiguchi, A. Kuroda, H. Ohtake
  • Journal Title: J. Biosci. Bioeng 106 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem. (2008)
  • Author(s): J. Kato, et al.
  • Journal Title: Journal of Bioscience and Bioengineering 106 Pages: 1-7
  • Peer Reviewed
• [Journal Article] 環境細菌の走化性-Pseudomonas中心に- (2007)
  • Author(s): 加藤純一, 金慧恩
  • Journal Title: J. Environ. Cell flameTCE mMmeCell per fla CellsperframeTime(s)WT (3. 8 mM)WT(0. 1 mM)Mutant (0. 1 mM)Mutant (3. 8 mM) Biotechnol 7 Pages: 93-98
  • Peer Reviewed
• [Journal Article] Ethylene chemotaxis in Pseudomonas aeruginosa and other Pseudomonas species. (2007)
  • Author(s): H.-E. Kim, M. Shitashiro, A. Kuroda, N. Takiguchi, J. Kato
  • Journal Title: Microbes Environment 22 Pages: 186-189
  • Peer Reviewed
• [Journal Article] Ethylene chemotaxis in Pseudomonas aeruginosa and other Pseudomonas species (2007)
  • Author(s): Hye-Eun Kim, et. al.
  • Journal Title: Microbes & Environments 22 Pages: 186-189
  • Peer Reviewed
• [Presentation] 植物関連物質に対するPseudomonas属細菌の走化性の分子機構 (2009)
  • Author(s): 桑野靖之, 米田佳那子, 中島田豊, 加藤純一
  • Organizer: 日本微生物生態学会第25回大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-11-22
• [Presentation] 植物関連物質に対する Psdeudomonas 属細菌の走化性の分子機構 (2009)
  • Author(s): 桑野靖之, 米田佳那子, 中島田豊, 加藤純一
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島大学工学部
  • Year and Date: 2009-11-22
• [Presentation] Pseudomonas putida F1のトリクロロエチレン走化性センサーの解析 (2009)
  • Author(s): 加藤純一, 林田通世, 中島田豊
  • Organizer: 日本生物工学会2009年度大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Presentation] Psuedomonas putida F1のトリクロロエチレン走化性センサーの解析 (2009)
  • Author(s): 加藤純一, 林田通世, 中島田豊
  • Organizer: 日本生物工学会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 2009-09-24
• [Presentation] Chemotactic transducer proteins for environmental pollutants in Pseudomonas aeruginosa PAO1. (2008)
  • Author(s): J. Kato, H. -E. Kim, N. Takiguchi
  • Organizer: International Biotechnology Symposium/Exhibitio
  • Place of Presentation: Dalian, China
  • Year and Date: 2008-10-14
• [Presentation] Chemotaxis towards environmental pollutants in Pseudomonas aeruginosa (2008)
  • Author(s): J. Kato, et al.
  • Organizer: International Biotechnology Symposium & Exhibition
  • Place of Presentation: 大連、中国
  • Year and Date: 2008-10-14
• [Presentation] Chemotactic transducer proteins for plant-related compounds in Pseudomonas aeruginosa. (2008)
  • Author(s): J. Kato, H. -E. Kim, H. Dabasaki
  • Organizer: International Union of Microbiology Societies 2008
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2008-08-06
• [Presentation] Chemotactic transducer proteins for plant-related compounds in Pseudomonas aeruginosa (2008)
  • Author(s): J. Kato, et al.
  • Organizer: International Union of Microbiology Society Conference
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2008-08-06
• [Presentation] TCE分解細菌のTCE走化性センサーの発見 (2008)
  • Author(s): 金慧恩、他
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27
• [Presentation] Molecular analysis of bacterial chemotaxis toward plant-related compounds. (2007)
  • Author(s): H.E. Kim, J. Kato
  • Organizer: 2007 International Meeting of the Federation of Korean Microbiological Societies
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-11
• [Presentation] Motile bacteria can sense and move to environmental pollutants. (2007)
  • Author(s): H.E. Kim, J. Kato
  • Organizer: 2007 International Meeting of the Federation of Korean Microbiological Societies
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-11
• [Presentation] Pseudomonas aruginosa環境汚染物質トリクロロエチレンとトルエンに対する走化性の分子機構 (2007)
  • Author(s): 金慧恩, 黒田章夫, 滝口昇, 加藤純一
  • Organizer: 日本生物工学会2007年度大会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-26
• [Presentation] Pseudomonas aeruginsaの植物関連物質への走化性の分子機構 (2007)
  • Author(s): 金慧恩, 黒田章夫, 滝口昇, 加藤純一
  • Organizer: 日本生物工学会2007年度大会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-26
• [Presentation] Pseudomonas aeruginosaの植物関連物質への走化性の分子機構 (2007)
  • Author(s): 金慧恩、他
  • Organizer: 日本生物工学会2007年度大会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-26
• [Book] Pseudomonas Motility and Chemotaxis(H. A. Rehm ed.) (2008)
  • Author(s): J. Kato
  • Publisher: Wiley-VCH Verlag GmbH & Co. Weinheim
• [Book] Pseudomonas (2008)
  • Author(s): J. Kato
  • Total Pages: 350
  • Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
• [Book] Pseudomonas - Model Organism, Pathogen, Cell Factory (2008)
  • Author(s): Junichi Kato
  • Publisher: Wiley-VCH
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/mbiotech/kato_lab/index.html