| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Research Abstract |
This study approaches the finding of novel factors that interact with ubiquitination-related proteins by monitoring the rotational motion of proteins. First, I established the experimental procedure to quantify the rotational motion of membrane lipids in yeast. The defect in ergosterol biosynthesis resulted in a decrease in membrane rigidity and an increase in the rotational lipid motion. The measurement of fluorescein-labeled model proteins suggests that this technique is valid for proteins of less than 10 kDa. Sna3 was identified as a factor that potentially interacted with Rsp5 ubiquitin ligase. In the future study, I investigate the molecular interaction between Rsp5 and Sna3 using the system employed in this study.
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