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Biosyntheses and molecular functions of novel biofactors, D-amino acids : from microorganisms to mammals

Research Project

Project/Area Number 19380059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionNagoya University

Principal Investigator

YOSHIMURA Tohru  Nagoya University, 大学院・生命農学研究科, 教授 (70182821)

Co-Investigator(Kenkyū-buntansha) HEMMI Hisashi  名古屋大学, 大学院・生命農学研究科, 准教授 (60302189)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
KeywordsD-アミノ酸 / D-セリンデヒドラターゼ / D-セリン定量 / ピリドキサルリン酸 / スクレイピー / 細胞性粘菌 / Saccharomyces cerevisiae / Escherichia coli / D-セリン / セリンラセマーゼ / カイコ / 出芽酵母
Research Abstract

In this study, we found a novel D-serine dehydratase in Saccharomyces cerevisiae, and studied its properties. With this enzyme exhibiting high substrate specificity for D-serine, we constructed a novel D-serine assay system. We examined the relationship between the concentration of D-amino acids in the various organs of a scrapy-infected hamster and the progress in the disease. We also studied the D-serine metabolism in a slime cellular mold, Dictyostellium discoidium, to understand the role of D-serine in development, and found that the cell contains serine racemase catalyzing D-serine synthesis, and D-serine dehydratase and D-amino acid oxidase catalyzing D-serine degradation. In this study, we also examine the function of E.coli YggS, a homolog of the unknown mammalian protein, PROSC, which exhibits the similarity in the primary structure with the bacterial alanine racemase.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (34 results)

All 2010 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (16 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation.2009

    • Author(s)
      Ito T, Uozumi N, Nakamura T, Takayama S, Matsuda N, Aiba H, Hemmi H, Yoshimura T.
    • Journal Title

      Biosci Biotechnol Biochem. 73

      Pages: 2698-2704

    • NAID

      10027549163

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serine racemase with catalytically active lysinoalanyl residue.2009

    • Author(s)
      Yamauchi T, Goto M, Wu HY, Uo T, Yoshimura T, Mihara H, Kurihara T, Miyahara I, Hirotsu K, Esaki N.
    • Journal Title

      J Biochem. 145

      Pages: 421-424

    • NAID

      10026301897

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Implication of YggT of Escherichia coli in osmotic regulation2009

    • Author(s)
      Ito T.
    • Journal Title

      Biosci.Biochem.Biotech. 73

      Pages: 2698-2704

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a homolog of mammalian serine racemase from Schizosaccharomyces pombe2009

    • Author(s)
      Goto M.
    • Journal Title

      J.Biol.Chem. 284

      Pages: 25944-25952

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serine racemase with catalytically active lysinoalanyl residue2009

    • Author(s)
      Yamauchi T.
    • Journal Title

      J.Biochem. 145

      Pages: 421-424

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] D-Amino acids in the brain: structure and function of pyridoxal phosphate-dependent amino acid racemases.2008

    • Author(s)
      Yoshimura T, Goto M.
    • Journal Title

      FEBS J. 275

      Pages: 3527-3537

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae.2008

    • Author(s)
      Ito T, Hemmi H, Kataoka K, Mukai Y, Yoshimura T.
    • Journal Title

      Biochem J. 409

      Pages: 399-406

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] D-Amino acids in the brain : structure and function of pyridoxal phosphate-dependent amino acid racemases2008

    • Author(s)
      Yoshimura T., Goto, M.
    • Journal Title

      FEBS J. 275

      Pages: 3527-3537

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アミノ酸ラセマーゼの構造と機能2008

    • Author(s)
      吉村徹
    • Journal Title

      生化学 80

      Pages: 324-330

    • NAID

      10021191626

    • Related Report
      2008 Annual Research Report
  • [Journal Article] A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae2008

    • Author(s)
      Ito, T.
    • Journal Title

      Biochem. J. 409

      Pages: 399-406

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae.2007

    • Author(s)
      Ito T, Takahashi K, Naka T, Hemmi H, Yoshimura T.
    • Journal Title

      Anal Biochem. 371

      Pages: 167-172

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic assay of D-serine using D-serine dehydratase from Saccharom yces cerevisiae.2007

    • Author(s)
      Ito, T.
    • Journal Title

      Anal. Biochem. 371

      Pages: 167-172

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mode of action of a germination-specific cortex-lytic enzyme, SleC, of Clostridium perfringens S40.2007

    • Author(s)
      Kumazawa, T.
    • Journal Title

      Biosci. Siotechnol. Siochem. 71

      Pages: 884-892

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 疾患マーカーとしてのD-アミノ酸と微生物酵素によるその検出法の開発2010

    • Author(s)
      吉村徹
    • Organizer
      日本薬学会第130年会シンポジウム
    • Place of Presentation
      就実大学、岡山
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] D-Amino acids as disease marker and construction of their detection system2009

    • Author(s)
      Tohru Yoshimura
    • Organizer
      Italy-Japan Symposium New Trends in Enzyme Science and Technology
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2009-10-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Eukaryotic D-serine dehydratase from Saccharomyces cerevisiae2009

    • Author(s)
      Tohru Yoshimura
    • Organizer
      The first international conference of D-amino acid research
    • Place of Presentation
      淡路島
    • Year and Date
      2009-07-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Eukaryotic D-serine dehydratase from Saccharomyces cerevisiae2009

    • Author(s)
      吉村徹
    • Organizer
      The first international conference of D-amino acid research
    • Place of Presentation
      Awajishima, Hyogo, Japan
    • Year and Date
      2009-07-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Eucaryotic D-serine dehydratase and its application to D-serine assay2009

    • Author(s)
      Tohru Yoshimura
    • Organizer
      Recent Trends in Bioconvergence Technology
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2009-06-09
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Saccharomyces cerevisiae D-セリンデヒドラターゼの反応機構2009

    • Author(s)
      吉村徹
    • Organizer
      第61回日本ビタミン学会大会
    • Place of Presentation
      京都学園大学、京都
    • Year and Date
      2009-05-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] E. coliの新奇ビタミンB6タンパク質、YggSの構造と機能2009

    • Author(s)
      伊藤智和, 吉村徹, 他
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞性粘菌、Dyctiostelium discoideumのD-アミノ酸代謝関連酵素2008

    • Author(s)
      諸橋直也, 邊見久, 吉村徹, 他
    • Organizer
      日本分子生物学会年会・日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 真核生物型D-セリンデヒドラターゼの触媒機構 : 亜鉛の機能と触媒残基の同定2008

    • Author(s)
      伊藤智和, 邊見久, 吉村徹, 他
    • Organizer
      日本分子生物学会年会・日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコ変態時のD-セリン動態に関する研究2008

    • Author(s)
      袴田真穂, 伊藤智和, 邊見久, 吉村徹, 他
    • Organizer
      日本分子生物学会年会・日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae2008

    • Author(s)
      Ito, T., Yoshimura, T., 他
    • Organizer
      2nd International Interdisciplinary Conference on Vitamins, Coenzymes and Biofactors
    • Place of Presentation
      Athens, Georgea, USA
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enzymology and application of D-serine-related enzymes.2008

    • Author(s)
      Tohru Yoshimura
    • Organizer
      4th apan-Finland Biotechnology Symposium.
    • Place of Presentation
      扎幌
    • Year and Date
      2008-10-02
    • Related Report
      2009 Final Research Report
  • [Presentation] A novel Zinc-dependent D-serine dehydratase of Saccharomyces cerevisiae and its application to enzymatic assay of D-serine.2008

    • Author(s)
      Tohru Yoshimura
    • Organizer
      JSPS-NOW Joint Seminar on The 4th Japan-Netherland Joint Seminar on Enzymatic Science and Biotechnology.
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 病態解析を目的としたD-セリン酵素定量法の開発2008

    • Author(s)
      中朋子, 伊藤智和, 邊見久, 吉村徹
    • Organizer
      D-アミノ酸研究会学術講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞生粘菌セリンラセマーゼの触媒機構2008

    • Author(s)
      伊藤智和, 邊見久, 吉村徹, 他
    • Organizer
      D-アミノ酸研究会学術講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 真核生物型D-セリンデヒドラターゼの触媒機構2008

    • Author(s)
      伊藤智和
    • Organizer
      2008年度日本農芸化学会大会
    • Place of Presentation
      名古屋市(名城大学)
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~bmm/yoshimura/index.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] D-アミノ酸測定法2009

    • Inventor(s)
      吉村徹, 加藤志郎
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      2009-241924
    • Filing Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] D-セリンデヒドラターゼ及びその利用2009

    • Inventor(s)
      吉村徹, 伊藤智和
    • Industrial Property Rights Holder
      名古屋大学
    • Filing Date
      2009-03-21
    • Related Report
      2008 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] D-セリンデヒドラターゼ及びその利用

    • Inventor(s)
      吉村徹、伊藤智和
    • Industrial Property Rights Holder
      名古屋大学
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] D-アミノ酸測定法

    • Inventor(s)
      吉村徹、加藤志郎
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Number
      2009-241924
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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