Study on mechanism of regulation of brain aging by functional polyphenols

• Project/Area Number: 19380073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Nagoya University
• Principal Investigator: OSAWA Toshihiko Nagoya University, 大学院・生命農学研究科, 教授 (00115536)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2008: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2007: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: アルツハイマー症 / パーキンソン症 / 脳内老化 / 酸化ストレス / ドパミン神経細胞 / 抗酸化ポリフェノール / ゴマリグナン / セサミノールカテコール / ドパミン / ドコサヘキサエン酸 / アラキドン酸 / Aβタンパク質 / パーキンソン病 / α-シヌクレイン / クルクミン / テトラヒドロクルクミン / 脂質過酸化物 / FOXO

Research Abstract

神経変性疾患であるアルツハイマー症やパーキンソン病の発症における酸化ストレスの関与の解明のために、脳内の主要な多価不飽和脂肪酸であるドコサヘキサエン酸(DHA)やアラキドン酸(ARA)由来の脂質ヒドロペルオキシドに特異的なモノクローナル抗体の作製とLC-MS/MS による有機化学的な解析法の確立を行った。さらに、炎症反応に特異的なバイオマーカーの検索とモノクローナル抗体の作製を行うと共に、「抗酸化ポリフェノール」による抑制機構の解明を行った。

Research Products

• [Journal Article] Quantification of Modified Tyrosines in Healthy and Diabetic Human Urine using Liquid Chromatography/Tandem Mass Spectrometry (2009)
  • Author(s): Kato, Y., Dozaki, N., Nakamura, T., Kitamoto, N., Yoshida, A., Naito, M., Kitamura, M., Osawa, T.
  • Journal Title: J. Clin. Biochem. Nutr 44 Pages: 67-78
  • Peer Reviewed
• [Journal Article] Astaxanthin inhibits reactive oxygen species-mediated cellular toxicity in dopaminergic SH-SY5Y cells via mitochondria-targeted protective mechanism (2009)
  • Author(s): Liu, X., Shibata, T., Hisaka, S., Osawa, T.
  • Journal Title: Brain Res 1254 Pages: 18-27
  • Peer Reviewed
• [Journal Article] Quantification of Modified Tyrosines in Healthy and Diabetic Human Urine using Liquid Chromatography/Tandem Mass Spectrometry (2009)
  • Author(s): Kato Y., et al.
  • Journal Title: J Clin Biochem Nutr. 44(1) Pages: 67-78
  • Peer Reviewed
• [Journal Article] Astaxanthin inhibits reactive oxygen species-mediated cellular toxicity in dopaminergic SH-SY5Y cells via mitochondria-targeted protective mechanism (2009)
  • Author(s): Liu X, et al.
  • Journal Title: Brain Res. 1254 Pages: 18-27
  • Peer Reviewed
• [Journal Article] Formation of dopamine adducts derived from brain polyunsaturated fatty acids : Mechanism for Parkinson's disease (2008)
  • Author(s): Liu, X., Yamada, Y., Maruyama, W., Osawa, T.
  • Journal Title: J. Biol. Chem 283 Pages: 34887-34895
  • Peer Reviewed
• [Journal Article] DHA hydroperoxides as a potential inducer of neuronal cell death : A mitochondrial dysfunction-mediated pathway (2008)
  • Author(s): Liu, X., Shibata, T., Hisaka, S., Kawai, Y., Osawa, T.
  • Journal Title: J. Clin. Biochem. Nutr 43 Pages: 26-33
  • Peer Reviewed
• [Journal Article] 食品機能評価におけるバイオマーカーの重要性 (2008)
  • Author(s): 大澤俊彦
  • Journal Title: 日薬理誌(Folia Pharmacol. Jpn.) 132 Pages: 140-144
  • NAID: 10024383722
• [Journal Article] Formation of dopamine adducts derived from brain polyunsaturated fatty acids : Mechanism for Parkinson's disease (2008)
  • Author(s): Liu X, et al.
  • Journal Title: J. Biol. Chem. 283(50) Pages: 34887-34895
  • Peer Reviewed
• [Journal Article] DHA hydroperoxides as a potential inducer of neuronal cell death : A mitochondrial dysfunction-mediated pathway (2008)
  • Author(s): Liu X, et al.
  • Journal Title: J. Clin. Biochem. Nutr. 43(1) Pages: 26-33
  • Peer Reviewed
• [Journal Article] 食品機能評価におけるバイオマーカーの重要性 (2008)
  • Author(s): 大澤俊彦
  • Journal Title: 日薬理誌(Folia Pharmacol. Jpn. ) 132 Pages: 140-144
  • NAID: 10024383722
• [Journal Article] The effects of tetrahydrocurcumin and green tea polyphenol on the survival of male C57BL/6 mice (2007)
  • Author(s): Kitani, K., Osawa, T., Yokozawa, T.
  • Journal Title: Biogerontology 8 Pages: 567-573
  • Peer Reviewed
• [Journal Article] In parkinsonian substantia nigra, alpha-synuclein is modified by acrolein, a lipid-peroxidation product, and accumulates in the dopamine neurons with inhibition of proteasome activity. (2007)
  • Author(s): Shamoto-Nagai, M., Maruyama, W., Hashizume, Y., Yoshida, M., Osawa, T., Riederer, P., Naoi, M.
  • Journal Title: J. Neural. Transm 114 Pages: 1559-1567
  • Peer Reviewed
• [Journal Article] In parkinsonian substantia nigra, alpha-synuclein is modified by acrolein, a lipid-peroxidation product, and accumulates in the dopamine neurons with inhibition of proteasome activity (2007)
  • Author(s): Shamoto-Nagai, M., et al.
  • Journal Title: J. Neural. Transm 114 Pages: 1559-1567
  • Peer Reviewed
• [Journal Article] The effects of tetrahydrocurcumin and green tea polyphenol on the survival of male C57BL/6 mice (2007)
  • Author(s): Kitani, K., et al.
  • Journal Title: Biogerontology 8 Pages: 567-573
  • Peer Reviewed
• [Presentation] Development of "Antibody Chip" by Application of Monoclonal Antibody Specific to Oxidative Stress Biomarkers (2008)
  • Author(s): Osawa T
  • Organizer: 14^<th> International Conference of Society of Free Radical Research
  • Place of Presentation: China
  • Year and Date: 2008-10-19
• [Presentation] Development of "Antibody Chip" by Application of Monoclonal Antibody Specific to Oxidative Stress Biomarkers (2008)
  • Author(s): Osawa T.
  • Organizer: 14th International Conference of Society of Free Radical Research
  • Place of Presentation: China
  • Year and Date: 2008-10-19
• [Presentation] Development of Novel Functional Materials by Fermentation of Antioxidative Phytochemicals (2007)
  • Author(s): Osawa, T
  • Organizer: 2nd Verona International Meeting on Foods, Phytotherapeutic Compounds and Health
  • Place of Presentation: Italy
  • Year and Date: 2007-09-30
• [Presentation] Development of Novel Functional Materials by Fermentation of A ntioxidative Phytochemicals (2007)
  • Author(s): Osawa, T.
  • Organizer: 2nd Verona International Meeting on Foods, Phytotherapeutic Compounds and Health
  • Place of Presentation: Italy
  • Year and Date: 2007-09-30
• [Book] サプリメントの基本4-ポリフェノール。アンチエイジング医学の基礎と臨床(日本抗加齢医学会、専門医・指導士認定委員会) (2008)
  • Author(s): 大澤俊彦
  • Publisher: メジカルビュー社
• [Book] 抗酸化成分の役割とフィトケミカルへの期待。アンチエイジング・ヘルスフード-抗加齢・疾病予防・健康寿命延長への応用-(水島裕監修, 青木晃, 白澤卓二, 矢澤一良, 米井嘉一編集) (2008)
  • Author(s): 大澤俊彦
  • Publisher: (株)サイエンスフォーラム
• [Book] アンチエイジング医学の基礎と臨床(日本抗加齢医学会、専門医・指導土認定委員会) (2008)
  • Author(s): 大澤俊彦
  • Total Pages: 455
  • Publisher: メジカルビュー社
• [Book] アンチエイジング・ヘルスフード-抗加齢・疾病予防・健康寿命延長への応用-(水島裕監修、青木晃、白澤卓二、矢澤一良、米井嘉一編集) (2008)
  • Author(s): 大澤俊彦
  • Total Pages: 463
  • Publisher: (株)サイエンスフォーラム
• [Book] バイオマーカーの開発。抗肥満食品・素材の開発と応用展開-メタボリックシンドロームにおけるバイオマーカーの確立と応用-(大澤俊彦監修) (2007)
  • Author(s): 大澤俊彦
  • Publisher: シーエムシー出版
• [Book] 抗肥満食品・素材の開発と応用展開-メタボリックシンドロームにおけるバイオマーカーの確立と応用-(大澤俊彦監修) (2007)
  • Author(s): 大澤俊彦
  • Total Pages: 297
  • Publisher: (株)シーエムシー出版