Basic study on rapid detection and control of pathogenic Listeria monocytogenes

• Project/Area Number: 19380076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: MIYAMOTO Takahisa Kyushu University, 大学院・農学研究院, 教授 (70190816)
• Co-Investigator(Kenkyū-buntansha): HONJOH Ken0ichi 九州大学, 大学院・農学研究院, 准教授 (00264101)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 食品安全性 / Listeria monocytogenes / リアルタイムPCR / 病原性 / hlyA / clpC / plcA / inlA

Research Abstract

Listeria monocytogenes (リステリア菌)は, 食中毒細菌で, 低温増殖性であることから今後, 大規模な食中毒発生の懸念がある。本菌を迅速に検出し, 効果的に制御する必要があるので, 食品と環境から分離されたリステリア菌合計192 株の遺伝子解析を行い, 遺伝子型と病原性の強さとの関係を調べ, リアルタイムPCR を用いたサイクリングプローブ法により, 病原性と推定される菌株を特異的および簡易迅速に検出する方法を開発した。

Research Products

• [Journal Article] Subtyping of Listeria monocytogenes based on nucleotide polymorphism in the clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates (2008)
  • Author(s): K.HONJOH, K.FUJIHARA, T.HARAGUCHI, Y. ONO, H.KOBAYASHI, H.HIWAKI, H.KAMIKADO, Sung Sik JANG, Sangryeol RYU, and T.MIYAMOTO
  • Journal Title: Food Science and Technology Research Vol.14 Pages: 557-564
  • NAID: 10024489620
  • Peer Reviewed
• [Journal Article] Subtyping of Listeria monocytogenes based on nucleotide polymorphism in the clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates (2008)
  • Author(s): Kenichi HONJOH, et al.
  • Journal Title: Food Science and Technology Research 14(6) Pages: 557-564
  • Peer Reviewed
• [Presentation] L. monocytogenesの病原性と分類に関する研究 (2009)
  • Author(s): 水江裕美, 宮本敬久
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-03-28
• [Presentation] Studies on subtyping and pathogenicity of listeria monocytogenes (2008)
  • Author(s): Liu Pei, T. Miyamoto, et al.
  • Organizer: 14th World Congress of Food Science & Technology
  • Place of Presentation: Shanghai, CHINA
  • Year and Date: 2008-10-21
• [Presentation] Studies on subtyping and pathogenicity of listeria monocytogenes (2008)
  • Author(s): Liu Pei, K. Fujihara, H. Mizue, K. Honjoh and T. Miyamoto
  • Organizer: 14th World Congress of Food Science & Technology
  • Place of Presentation: Shanghai, China.
  • Year and Date: 2008-10-17
• [Presentation] Intraspecific lineage group identification of Listeria monocytogenes based on DNA sequences of some genes and rapid detection of L. monocytogenes genetically similar to clinical isolates (2007)
  • Author(s): Takahisa Miyamoto, Kumiko Fujihara, Ken-ichi, Honjoh, Hideaki Kamikado, Takashi Tanaka
  • Organizer: Natural Resources, Food and Agriculture Panel, 36th Annual meeting
  • Place of Presentation: Ibaraki,Japan.
  • Year and Date: 2007-10-23
• [Presentation] L.monocytogenesの分類と制御に関する研究 (2007)
  • Author(s): 藤原 久美子
  • Organizer: 日本農芸化学会中四国・西日本支部合同大会
  • Place of Presentation: 山口大学(山口市)
  • Year and Date: 2007-09-15
• [Presentation] L. monocytogenes の分類と制御に関する研究 (2007)
  • Author(s): 藤原久美子, 原口敬寛, 樋脇弘, 本城賢一, 宮本敬久
  • Organizer: 日本農芸化学会中四国・西日本支部合同大会
  • Place of Presentation: 山口市, 山口大学