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Basic study on rapid detection and control of pathogenic Listeria monocytogenes

Research Project

Project/Area Number 19380076
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionKyushu University

Principal Investigator

MIYAMOTO Takahisa  Kyushu University, 大学院・農学研究院, 教授 (70190816)

Co-Investigator(Kenkyū-buntansha) HONJOH Ken0ichi  九州大学, 大学院・農学研究院, 准教授 (00264101)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Keywords食品安全性 / Listeria monocytogenes / リアルタイムPCR / 病原性 / hlyA / clpC / plcA / inlA
Research Abstract

Listeria monocytogenes (リステリア菌)は, 食中毒細菌で, 低温増殖性であることから今後, 大規模な食中毒発生の懸念がある。本菌を迅速に検出し, 効果的に制御する必要があるので, 食品と環境から分離されたリステリア菌合計192 株の遺伝子解析を行い, 遺伝子型と病原性の強さとの関係を調べ, リアルタイムPCR を用いたサイクリングプローブ法により, 病原性と推定される菌株を特異的および簡易迅速に検出する方法を開発した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (8 results)

All 2009 2008 2007

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Subtyping of Listeria monocytogenes based on nucleotide polymorphism in the clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates2008

    • Author(s)
      K.HONJOH, K.FUJIHARA, T.HARAGUCHI, Y. ONO, H.KOBAYASHI, H.HIWAKI, H.KAMIKADO, Sung Sik JANG, Sangryeol RYU, and T.MIYAMOTO
    • Journal Title

      Food Science and Technology Research Vol.14

      Pages: 557-564

    • NAID

      10024489620

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subtyping of Listeria monocytogenes based on nucleotide polymorphism in the clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates2008

    • Author(s)
      Kenichi HONJOH, et al.
    • Journal Title

      Food Science and Technology Research 14(6)

      Pages: 557-564

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] L. monocytogenesの病原性と分類に関する研究2009

    • Author(s)
      水江裕美, 宮本敬久
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡市
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Studies on subtyping and pathogenicity of listeria monocytogenes2008

    • Author(s)
      Liu Pei, T. Miyamoto, et al.
    • Organizer
      14th World Congress of Food Science & Technology
    • Place of Presentation
      Shanghai, CHINA
    • Year and Date
      2008-10-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] Studies on subtyping and pathogenicity of listeria monocytogenes2008

    • Author(s)
      Liu Pei, K. Fujihara, H. Mizue, K. Honjoh and T. Miyamoto
    • Organizer
      14th World Congress of Food Science & Technology
    • Place of Presentation
      Shanghai, China.
    • Year and Date
      2008-10-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Intraspecific lineage group identification of Listeria monocytogenes based on DNA sequences of some genes and rapid detection of L. monocytogenes genetically similar to clinical isolates2007

    • Author(s)
      Takahisa Miyamoto, Kumiko Fujihara, Ken-ichi, Honjoh, Hideaki Kamikado, Takashi Tanaka
    • Organizer
      Natural Resources, Food and Agriculture Panel, 36th Annual meeting
    • Place of Presentation
      Ibaraki,Japan.
    • Year and Date
      2007-10-23
    • Related Report
      2008 Final Research Report
  • [Presentation] L.monocytogenesの分類と制御に関する研究2007

    • Author(s)
      藤原 久美子
    • Organizer
      日本農芸化学会中四国・西日本支部合同大会
    • Place of Presentation
      山口大学(山口市)
    • Year and Date
      2007-09-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] L. monocytogenes の分類と制御に関する研究2007

    • Author(s)
      藤原久美子, 原口敬寛, 樋脇弘, 本城賢一, 宮本敬久
    • Organizer
      日本農芸化学会中四国・西日本支部合同大会
    • Place of Presentation
      山口市, 山口大学
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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