Analytical studies on functions and biosynthesis of metabolism-controlling hydrophobic factors produced by white rot fungus

• Project/Area Number: 19380100
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 林産科学・木質工学
• Research Institution: Kyoto University
• Principal Investigator: WATANABE Takashi Kyoto University, 生存圏研究所, 教授 (80201200)
• Co-Investigator(Kenkyū-buntansha): HONDA Yoichi 京都大学, 生存圏研究所, 准教授 (70252517) ; WATANABE Takahito 京都大学, 生存圏研究所, 助教 (30362403)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: バイオサーファクタント / 白色腐朽菌 / リグニン / バイオマス / 脂質過酸化 / 代謝物

Research Abstract

We analyzed structure and functions of amphipathic metabolites produced by a selective white rot fungus, Ceriporiopsis subvermispora, and found that this fungus secretes novel biosurfactants and amphipathic dicarboxylic acids with a new side chain structure, under the conditions producing slimy sheath composed of β-1,3 glucan. We also elucidated that production of the amphipathic compounds was increased under the wood rotting conditions which promote lignin biodegradation.

Research Products

• [Journal Article] Characterization of aΔ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus (2010)
  • Author(s): Takahito Watanabe, Saeko Tsuda, Hiroshi Nishimura, Yoichi Honda, Takashi Watanabe
  • Journal Title: Applied Microbiology and Biotechnology
  • Peer Reviewed
• [Journal Article] Characterization of a Δ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus (2010)
  • Author(s): Takahito Watanabe, Saeko Tsuda, Hiroshi Nishimura, Yoichi Honda, Takashi Watanabe
  • Journal Title: Applied Microbiology and Biotechnology
  • Peer Reviewed
• [Journal Article] Absolute configuration of ceriporic acids, the iron redox-silencing metabolites produced by a selective lignin-degrading fungus, Ceriporiopsis subvermispora (2009)
  • Author(s): Hiroshi Nishimura,, Kyoko Murayama, Takahito Watanabe, Yoichi Honda, Takashi Watanabe
  • Journal Title: Chem. Phys. Lipids 159 Pages: 77-80
  • Peer Reviewed
• [Journal Article] Absolute configuration of ceriporic acids, the iron redox-silencing metabolites produced by a selective lignin-degrading fungus, Ceriporiopsis subvermispora (2009)
  • Author(s): Nishimura, H., K.Murayama, T.Watanabe, Y.Honda, T.Watanabe
  • Journal Title: Chem.Phys.Lipids 159 Pages: 77-80
  • Peer Reviewed
• [Journal Article] De novo synthesis of (Z)-and (E)-7-hexadecenylitaconic acids by a selectivelignin-degraing fungus, Ceriporiopsis subvermispora (2008)
  • Author(s): Nishimura, H., S. Tsuda, H. Shimizu, Y, Ohashi., T. Watanabe, Y. Honda, T. Watanabe
  • Journal Title: Phytochemistry 69 Pages: 2593-2602
  • Peer Reviewed
• [Journal Article] Redox ssilencin of the Fenton reaction system by an alkylitaconic acid, ceriporic acid B produced by a selective ligni-ddegrading fungus, Ceriporiopsis subvermispora (2007)
  • Author(s): Ohashi, Y., Y. Kan, T. Watanabe, Y. Honda, T. Watanabe
  • Journal Title: Org. Biomol. chem 5 Pages: 840-847
  • Peer Reviewed
• [Journal Article] Biotechnological wood modification with selective white-rot fungi and its molecular mechanisms. 45, 269-276 (2007). (2007)
  • Author(s): Fackler, K., C. Gradinger, M. Schmutzer, C. Tavzes, I. Burgert, M. Schwanninger, B. Hinterstoisser, T. Watanabe, K. Messne
  • Journal Title: Food Technol. Biotechnol 45 Pages: 269-276
  • Peer Reviewed
• [Journal Article] 選択的白色腐朽菌によるバイオマス変換と脂質関連ラジカル制御因子 (2007)
  • Author(s): 渡辺隆司
  • Journal Title: 木材保存 33 Pages: 102-116
  • NAID: 10019925898
  • Peer Reviewed
• [Presentation] Microbial and microwave-assisted degradation of lignin for lignocellulosic biorefinery (2010)
  • Author(s): 渡辺隆司
  • Organizer: Finnish-Japanese Workshop on Functional Material
  • Place of Presentation: ヘルシンキ、フィンランド
  • Year and Date: 2010-05-25
• [Presentation] Structural and functional analyses of secondary metabolites involved in extracellular glucan sheath produced by Ceriporiopsis subvermispora (2010)
  • Author(s): Hiroshi Nishimura, Takahito Watanabe, Yoichi Honda, Takashi Watanabe
  • Organizer: Lignobiotech one
  • Place of Presentation: フランス(ランス)
  • Year and Date: 2010-03-30
• [Presentation] Structural and functional analyses of secondary metabolites involved in extracellular glucan sheath produced by Ceriporiopsis subvermispora (2010)
  • Author(s): Hiroshi Nishimura, Takahito Watanabe, Yoichi Honda, Takashi Watanabe
  • Organizer: Lignobiotech one
  • Place of Presentation: ランス、フランス
  • Year and Date: 2010-03-30
• [Presentation] 選択的白色腐朽菌が産生するエポキシ化セリポリック酸関連代謝物の同定 (2010)
  • Author(s): 西村裕志, 佐々木碧, 瀬戸川雄一, 渡邊崇人, 本田与一, 渡辺隆司
  • Organizer: 日本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-18
• [Presentation] Microbial and microwave-assisted degradation of lignin for lignocellulosic biorefinery (2009)
  • Author(s): Takashi Watanabe
  • Organizer: Finnish-Japanese Workshop on Functional Material
  • Place of Presentation: フィンランド(ヘルシンキ)
  • Year and Date: 2009-05-25
• [Presentation] 選択的リグニン分解条件下における菌体外代謝物プロファイルと新規代謝物の同定 (2009)
  • Author(s): 村山京子, 西村裕志, 渡邊崇人, 本田与一, 渡辺隆司
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 選択的白色腐朽菌Ceriporiopsis subvermisporaが産生するsheathに関する研究 (2009)
  • Author(s): 鈴木大介、西村裕志、吉岡康一、渡邊崇人、本田与一、海田るみ、林隆久、高部圭司、渡辺隆司
  • Organizer: 日本木材学会大会
  • Place of Presentation: 松本
  • Year and Date: 2009-03-15
• [Presentation] セルロース系バイオリファイナリーに向けてのリグニン分解系の開発 (2008)
  • Author(s): 渡辺隆司
  • Organizer: 日本応用糖質学会近畿支部大会
  • Year and Date: 2008-10-31
• [Presentation] 選択的リグニン分解菌が産生する菌体外脂質関連代謝物の構造解析 (2008)
  • Author(s): 西村裕志, 村山京子, 渡邊崇人, 本田与一, 渡辺隆司
  • Organizer: 第53回リグニン討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-30
• [Presentation] 木質バイオリファイナリーに向けての担子菌リグニン分解系の解析と応用 (2008)
  • Author(s): 渡辺隆司, 大橋康典, 西村裕志, 津田冴子, 月原多佳久, 渡邊崇人, 本田与一
  • Organizer: 日本農芸化学会関西支部大会
  • Year and Date: 2008-09-12
• [Presentation] 選択的白色腐朽菌Ceriporiopsis subvermisporaが産生する新規セリポリツク酸類縁体 (2008)
  • Author(s): 西村裕志、村山京子、長谷川隆大、清水仁恵、津田冴子、大橋康典、渡遵崇人、本田与一、渡辺隆司
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-03-27
• [Presentation] 選択的リグニン分解性担子菌の産生するバイオサーフアククント、 (2008)
  • Author(s): 西村裕志、渡遵崇人、本田与一、 渡辺隆司
  • Organizer: 第58回日本木材学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2008-03-19
• [Presentation] Extracellular lipid-related metabolites, ceriporic acids produced by a selective lignin-degrading fungus, Ceriporiopsis subvermispora (2008)
  • Author(s): Hiroshi Nishimura, Kyoko Murayama, Takahito Watanabe, Yoichi Honda, Takashi Watanabe
  • Organizer: Mie Bioforum 2008
  • Place of Presentation: Mie
• [Presentation] Lignin Biodegradation by Selective White Rot Fungi as a Biotechnological Tool for Lignocellulosic Biorefinery (2008)
  • Author(s): Takashi Watanabe, Hiroshi Nishimura, Saeko Tsuda, Rudianto Amirta, Yasunori Ohashi, Masafumi Oyadomari, Takahito Watanabe, Yoichi Honda
  • Organizer: Mie Bioforum 2008
  • Place of Presentation: Mie
• [Presentation] Lignin biodegradation by selective white rot fungi as biotechnological tool for lignocellulosic biorefinery. (2008)
  • Author(s): T. Watanahe, H. Nishimura, S. Tsuda, R. Amirta, Y. Ohashi, M, Oyadomari, T. Watanabe, Y. Honda
  • Organizer: MIE BIOFORUM 2008 Biotechnology of lignocellulose degradation
  • Place of Presentation: Mie
• [Presentation] 選択的リグニン分解菌が産生する新規代謝物ceriporic acid D (2007)
  • Author(s): 西村裕志, 清水仁恵, 津田冴子, 大橋康則, 渡邊崇人, 本田与一, 渡辺隆司
  • Organizer: 日本木材学会
  • Place of Presentation: 広島市
  • Year and Date: 2007-08-10
• [Presentation] Synthesis and structural analysis of ceriporic acids produced by a selective lignin-degrading fungus, Ceriporiopsis subvermispora (2007)
  • Author(s): Nishimura, H., H. Shimizu, S. Tsuda, Y. Ohashi, T. Watanabe, Y. Honda, T. Watanabe
  • Organizer: The 10th International Congress on Biotechnology in the Pulp and Paper Industry
  • Place of Presentation: マディソン、米国
• [Presentation] Redox silencing of the Fenton reaction system by an alkylitaconicacid, ceriporic acid B produced by a selective white rot fungus, Ceriporiopsis subvermispora (2007)
  • Author(s): Watanabe, T., Y. Ohashi, Y. Honda, T. Watanabe
  • Organizer: The 10th International Congress on Biotechnology in the Pulp and Paper Industry
  • Place of Presentation: マディソン、米国
• [Book] バイオマスハンドブック(第二版) (2010)
  • Author(s): 渡辺隆司
  • Total Pages: 523
  • Publisher: オーム社
• [Book] セルロース系バイオエタノール製造技術-食料クライシス回避のために- (2010)
  • Author(s): 渡辺隆司
  • Total Pages: 396
  • Publisher: エヌ・ティー・エス
• [Book] バイオマスハンドブック (第二版) (2010)
  • Author(s): 渡辺隆司
  • Total Pages: 523
  • Publisher: オーム社
• [Book] セルロース系バイオエタノール製造技術 -食料クライシス回避のために- (2010)
  • Author(s): 渡辺隆司
  • Total Pages: 396
  • Publisher: エヌ・ティー・エス
• [Book] In ACS Symposium Series 954, Materials, Chemicals and Energy from Forest Biomass (2007)
  • Author(s): Watanabe, Takashi, Y. Ohash, T. Tanabe, Takahito Watanabe, Y. Honda, K. Messner
  • Total Pages: 12
  • Publisher: Lignin biodegradation by selective white rot fungus and its potential use in wood biomass conversion