Wood identification by DNA analysis with micro-manipulation and direct PCR method.
Project/Area Number |
19380103
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
林産科学・木質工学
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Research Institution | Forestry and Forest Products Research Institute |
Principal Investigator |
ABE Hisashi Forestry and Forest Products Research Institute, 木材特性研究領域, 主任研究員 (80343812)
|
Co-Investigator(Kenkyū-buntansha) |
WATANABA Ugai 千葉工業大学, 工学部, 准教授 (70337707)
吉田 和正 独立行政法人森林総合研究所, 生物工学研究領域, 室長 (50353909)
|
Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Kazumasa 独立行政法人森林総合研究所, 生物工学研究領域, 室長 (50353909)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 木材 / 樹種識別 / 核酸 / 細胞・組織 / DNA / 細胞 / オルガネラ |
Research Abstract |
We investigated effects of long-term storage and high temperature treatment of wood to the DNA degradation and developed a method for extracting DNA effectively by ultrasonic treatment from wood. We applied the method for DNA analysis to species-level identification of wood products of Shorea spp. The usefulness of DNA analysis for wood identification at species level is demonstrated in this study.
|
Report
(4 results)
Research Products
(29 results)
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[Journal Article] Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products.2010
Author(s)
Tsumura Y., Kado T., Yoshida K., Abe H., Ohtani M., Taguchi Y., Fukue Y., Tani N., Ueno S., Yoshimura K., Kamiya K., Harada K., Takeuchi Y., Diway B., Finkeldey R., Na' iem M., Indrioko S., Ng K.K.S., Muhammad N., Lee S.L.
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Journal Title
NAID
Related Report
Peer Reviewed
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