Studies of fish vitellogenesis based on the multiple vitellogenin model

• Project/Area Number: 19380106
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: HARA Akihiko Hokkaido University, 大学院・水産科学研究院, 教授 (40091483)
• Co-Investigator(Kenkyū-buntansha): MATSUBARA Takahiro 独立行政法人水産総合研究センター, 海区水産業研究部, 室長 (60443389) ; SOYANO Kiyoshi 長崎大学, 東シナ海海洋環境資源研究センター, 教授 (80260735) ; TODO Takashi 北海道大学, 大学院・水産科学研究院, 准教授 (60303111) ; HIRAMATSU Naoshi 北海道大学, 大学院・水産科学研究院, 助教 (10443920)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: ビテロジェニン / 卵形成 / 卵黄タンパク質 / エストロジェン / 魚類 / 免疫測定法 / 卵黄蛋白質 / バイオマーカー / 環境毒性学 / 卵黄蛋白

Research Abstract

The objective of this study is to elucidate mechanisms involved in the yolk formation in fish oocytes. Results included development of novel technologies for separation and quantification of multiple subtypes of a yolk precursor (vitellogenin : Vg) ; such techniques were initially developed for the grey mullet, and thereafter applied for several other teleosts. Findings endow the better understandings on fish oogenesis, as well as improvement of fish egg quality in aquaculture and development of technologies for the surveys on the impacts of environmental xenoestrogenic chemicals.

Research Products

• [Journal Article] Development of evaluation systems for the detection of estrogenic endocrine disrupting chemicals (EDCs) in aquatic environments using estrogen-inducible biomarkers. (2010)
  • Author(s): Hiramatsu N, Luo W, Hong L, Soyano K, Aoki J, Amano H, Fujita T, Todo T, Matsubara T, Hara A.
  • Journal Title: Natl. Taiwan Mus. Spec. Pub. 14 Pages: 61-69
• [Journal Article] Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet(Mugil cephalus). (2010)
  • Author(s): Junya Aoki
  • Journal Title: Sci.Total Environment 408(3) Pages: 660-665
  • Peer Reviewed
• [Journal Article] Development of evaluation systems for the detection of estrogenic endocrine disrupting chemicals(EDCs)in aquatic environments using estrogen-inducible biomarkers. (2010)
  • Author(s): Naoshi Hiramatsu
  • Journal Title: National Taiwan Musium Special Publication 14 Pages: 49-56
• [Journal Article] Choriogenin and vitellogenin in red lip mullet (Chelon haematocheilus): purification, characterization, and evaluation as potential biomarkers for detecting estrogenic activity. (2009)
  • Author(s): Hong L, Fujita T, Wada T, Amano H, Hiramatsu N, Zhang X, Todo T, Hara A.
  • Journal Title: Comp Biochem Physiol 149C Pages: 9-17
• [Journal Article] Purification and classification of three lipovitellin subtypes in the marbled sole (Pleuronectes yokohamae). (2009)
  • Author(s): Amano H, Kitamura M, Fujita T, Hiramatsu N, Todo T, Hara A.
  • Journal Title: Zool Sci 26 Pages: 510-516
  • NAID: 40016772424
• [Journal Article] Purification and classification of three lipovitellin subtypes in the marbled sole(Pleuronectes yokohamae). (2009)
  • Author(s): Haruna Amano
  • Journal Title: Zoological Science 26 Pages: 510-516
  • Peer Reviewed
• [Journal Article] Conserved and variant molecular and functional features of multiple vitellogenins in white perch(Morone americana)and other teleosts. (2009)
  • Author(s): Reading J.Benjamin
  • Journal Title: Marine Biotechnology 11(2) Pages: 169-187
  • Peer Reviewed
• [Journal Article] Choriogenin and vitellogenin in red lip mullet (Chelon haematocheilus): purification, characterization, and evaluation as potential bilmarkers for detecting estrogenic activity (2009)
  • Author(s): Lei Hong
  • Journal Title: Comparative Biochemistry and Physiology 149C Pages: 9-17
  • Peer Reviewed
• [Journal Article] Multiple vitellogeninderived yolk proteins in gray mullet (Mugil cephalus): disparate proteolytic patterns associated with ovarian follicle maturation. (2008)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Kagawa H, Matsubara T, Sullivan CV, Hara A.
  • Journal Title: Mol Reprod Develop 75 Pages: 1307-1317
• [Journal Article] Purification and characterization of lipovitellin from Pacific saury Cololabis saira. (2008)
  • Author(s): Amano H, Kitamura M, Fujita T, Hiramatsu N, Todo T, Suyama S, Hara A.
  • Journal Title: Fish Sci 74 Pages: 830-836
  • NAID: 10021366940
• [Journal Article] Molecular alteration of three forms of vitellogenins and their product yolk proteins during oocyte growth and maturation in grey mullet (Mugil cephalus). (2008)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Kagawa H, Sawaguchi S, Matsubara T, Sullivan CV, Hara A.
  • Journal Title: CYBIUM Int J Ichthyol 32(2) Pages: 156-158
• [Journal Article] Differential production and uptake of dual vitellogenins in Japanese medaka (Oryzias latipes). (2008)
  • Author(s): Hiramatsu N, Inoue M, Ideuchi H, Fujita T, Amano H, Matsubara T, Sullivan CV, Hara A.
  • Journal Title: CYBIUM Int J Ichthyol 32(2) Pages: 260-260
• [Journal Article] Multiple vitellogenin-derived yolk proteins in gray mullet (Mugil cephalus): disparate proteolytic patterns associated with ovarian follicle maturatio (2008)
  • Author(s): Haruna Amano
  • Journal Title: Molecular Reproduction and Development 75 Pages: 1307-1317
  • Peer Reviewed
• [Journal Article] Purification and characterization of lipovitellin from Pacific saury Cololabis saira (2008)
  • Author(s): Haruna Amano
  • Journal Title: Fisheries Science 74 Pages: 830-836
  • Peer Reviewed
• [Journal Article] Molecular alteration of three forms of vitellogenins and their product yolk proteins during oocyte growth and maturation in grey mullet (Mugil cephalus) (2008)
  • Author(s): Haruna Amano
  • Journal Title: CYBIUM International Journal of Ichtyology 32 Pages: 156-158
  • Peer Reviewed
• [Journal Article] Differential production and uptake of dual vitellogenins in Japanese medaka (Oryzias latipes) (2008)
  • Author(s): Naoshi Hiramatsu
  • Journal Title: CYBIUM International Journal of Ichtyology 32 Pages: 260-260
  • Peer Reviewed
• [Journal Article] Egg yolk proteins in gray mullet (Mugil cephalus): purification and classification of multiple lipovitellins and other vitellogeninderived yolk proteins and molecular cloning of the parent vitellogenin genes. (2007)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Shimizu M, Sawaguchi S, Matsubara T, Kagawa H, Nagae M, Sullivan CV, Hara A.
  • Journal Title: J Exp Zool 307A Pages: 324-341
• [Journal Article] Purification of multiple vitellogenins in grey mullet (Mugil cephalus). (2007)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Sawaguchi S, Matsubara T, Sullivan CV, Hara A.
  • Journal Title: Mar Biol 152 Pages: 1215-1225
  • NAID: 120000950016
• [Journal Article] Egg yolk proteins in grey mullet(Mugil cephalus): purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes (2007)
  • Author(s): Haruna Amano
  • Journal Title: J. Experimental Zoology 307A Pages: 324-341
  • Peer Reviewed
• [Journal Article] Purification of multiple vitellogenins in grey mullet(Mugil cephalus). (2007)
  • Author(s): Haruna Amano
  • Journal Title: Marine Biology 152 Pages: 1215-1225
  • Peer Reviewed
• [Presentation] ヌタウナギの2型ビテロジェニン遺伝子のプロモーター領域解析 (2010)
  • Author(s): 西宮攻
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学藤沢校
  • Year and Date: 2010-03-27
• [Presentation] Development of bioassay for detecting aquatic estrogenic activities using red lip mullet. (2010)
  • Author(s): Wenshu Luo
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学藤沢校
  • Year and Date: 2010-03-27
• [Presentation] ヌタウナギの2型ビテロジェニン遺伝子のプロモーター領域解析. (2010)
  • Author(s): 西宮攻・國広康之・天野春菜・藤田敏明・稲川裕之・平松尚志・東藤孝・原彰彦
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学藤沢校, 藤沢
• [Presentation] メナダを用いた水圏エストロジェン活性検出法の確立. (2010)
  • Author(s): ラブンジュウ, ゴメイチン, ホンレイ, 平松尚志, 藤田敏明, 天野春菜, 東藤孝, 原彰彦.
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学藤沢校, 藤沢
• [Presentation] Estrogenic activities of coastal aquatic environments in China: evaluation using a model species, the red lip mullet (Chelon haematocheilus). (2009)
  • Author(s): Wu M, Aoki J, Hiramatsu N, Hara A, Soyano K, Zhong J.
  • Organizer: 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki, Japan.
• [Presentation] マコガレイの卵黄蛋白リポビテリンの検索と精製. (2009)
  • Author(s): 天野春菜・藤田敏明・平松尚志・東藤孝・原彰彦
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
• [Presentation] Estrogenic activities of coastal aquatic environments in China : evaluation using a model species, the red lip mullet(Chelon haematochelilus). (2009)
  • Author(s): Meqin Wu
  • Organizer: The 6^<th> International meeting on reproductive biology of aquatic animals of the East China sea
  • Place of Presentation: 長崎大学
• [Presentation] Yolk assembly in teleost: recent findings on the deposition of ovarian lipids and proteins. (2008)
  • Author(s): Hiramatsu N, Todo T, Ito T, Massaki K, Kasahara A, Amano H, Reading BJ, Matsubara T, Sawaguchi S, Sullivan CV, Hara A.
  • Organizer: World Aquaculture
  • Place of Presentation: Busan, Korea.
• [Presentation] Morecular characterization of hagfish vitellogenin. (2008)
  • Author(s): Kunihiro Y, Hiramatsu N, Fujita T, Amano H, Inagawa H, Todo T, Hara A.
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Jeju National University, Jeju, Korea.
• [Presentation] Preparation of recombinant antigens encoding a C-terminal peptide of complete vitellogenin subtypes in three-spined stickleback, Gasterosteus aculeatus. (2008)
  • Author(s): Takahashi M, Hiramatsu N, Fujita T, Amano H, Sawaguchi S, Matsubara T, Todo T, Hara A.
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Jeju National University, Jeju, Korea.
• [Presentation] Development of subtype-specific chemiluminescent immunoassay for vitellogenin in grey mullet. (2008)
  • Author(s): Amano H, Kotake A, Fujita T, Hiramatsu N, Todo T, Soyano K, Hara A.
  • Organizer: The 7th International Symposium of the 21st COE programs "Innovative Marine Life Science for three Es, Edibles, Environment and Education, in 21st Century"
  • Place of Presentation: Hokkaido University, Sapporo, Japan.
• [Presentation] ヌタウナギのビテロジェニンならびにその関連卵黄蛋白の検索および精製. (2008)
  • Author(s): 國弘康之・天野春菜・藤田敏明・稲川裕之・平松尚志・東藤孝・原彰彦
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 東海大学海洋学部, 静岡市
• [Presentation] イトヨの多型ビテロジェニンの精製. (2008)
  • Author(s): 高橋美咲・天野春菜・藤田敏明・平松尚志・東藤孝・松原孝博・原彰彦
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 東海大学海洋学部, 静岡市
• [Presentation] Effect of ethynylestradiol on sex differentiation in grey mullet (2008)
  • Author(s): Junya Aoki
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Cheju National University, Korea
• [Presentation] Molecular characterization of hagfish vitellogenin (2008)
  • Author(s): Yasuyuki Kunihiro
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Cheju National University, Korea
• [Presentation] Preparation of recombinant antigens encoding a C-terminal peptide of complete vitellogenins subtypes in three-spined stickleback, Gasterosteus aculeatus (2008)
  • Author(s): Misaki Takahashi
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Cheju National University, Korea
• [Presentation] Effect of ethynylestradiol on gonad in grey mullet Mugil cephalus (2008)
  • Author(s): Junya Aoki
  • Organizer: 5th World Fisheries Congress, Fisheries for Global Welfare and Environmental Conservation
  • Place of Presentation: 横浜市
• [Presentation] Monitoring estrogenic contaminations in aquatic environments using fish serum proteins (2008)
  • Author(s): Akihiko Hara
  • Organizer: The 7th International Symposium of the 21st COE programs "Innovative Marine Life Science for three Es, edibles, Environment and Education, in 21st Century"
  • Place of Presentation: 北海道大学札幌キャンパス
• [Presentation] Development of subtype-specific chemiluminescent immunoassay for vitellogenin in grey mullet (2008)
  • Author(s): Haruna Amano
  • Organizer: The 7th International Symposium of the 21st COE programs "Innovative Marine Life Science for three Es, edibles, Environment and Education, in 21st Century"
  • Place of Presentation: 北海道大学札幌キャンパス
• [Presentation] Purification and classification of egg yolk proteins derived from multiple vitellogenins in grey mullet (Mugil cephalus). (2007)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Matsubara T, Sullivan CV, Hara A.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint-Malo, France.
• [Presentation] Differential production and uptake of dual vitellogenins in Japanese medaka (Oryzias latipes). (2007)
  • Author(s): Hiramatsu N, Inoue M, Ideuchi H, Fujita T, Amano H, Matsubara T, Sullivan CV, Hara A.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint-Malo, France.
• [Presentation] Development of immunoassays for choriogenin and vitellogenin in red lip mullet, Chelon haematocheilus. (2007)
  • Author(s): 洪磊・和田竜典・藤田敏明・天野春菜・平松尚志・原彰彦
  • Organizer: 平成19年度日本水産学会秋期大会
  • Place of Presentation: 北海道大学水産学部
• [Presentation] Multiple vitellogenins and their derived yolk proteins in grey mullet (Mugil cephalus): differential proteolytic patterns during oocyte growth and maturation. (2007)
  • Author(s): Amano H, Fujita T, Hiramatsu N, Matsubara T, Sullivan CV, Hara A.
  • Organizer: 4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals
  • Place of Presentation: Nagasaki University, Nagasaki, Japan.
• [Presentation] Purification of vitellogenin and lipovitellin-like yolk protein in hagfish (Eptatretus burgeri). (2007)
  • Author(s): Kunihiro Y, Amano H, Fujita T, Inagawa H, Hiramatsu N, Todo T, Hara A.
  • Organizer: 4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals
  • Place of Presentation: Nagasaki University, Nagasaki, Japan.
• [Presentation] Multiple vitellogenins and their derived yolk proteins in grey mullet(Mugil cephalus): differential proteolytic patterns during oocyte growth and maturation. (2007)
  • Author(s): Haruna Amano
  • Organizer: 4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals
  • Place of Presentation: 長崎大学
• [Presentation] Puhrification and classlrication of egg yolk proteins dehved from multiple vitellogenins in grey mullet(Mugil cephalus) (2007)
  • Author(s): Haruna Amano
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish.
  • Place of Presentation: St. Malo, France
• [Presentation] Purification of multiple vitellogenins in three-spined stickleback(Garterosteus aculeatus). (2007)
  • Author(s): Misaki Takahashi
  • Organizer: 4th Japan-Korea, Korea‐Japan Joint Meeting on Reproductive Biology of Aquatic Animals
  • Place of Presentation: 長崎大学
• [Presentation] Monitoring of endocrine disruptant using serum protein as biomarkers. (2007)
  • Author(s): Akihiko Hara.
  • Organizer: International Workshop on the Evaluation of the 21st COE Program, "Marine Bio-manipulation Frontier for Food Production"
  • Place of Presentation: 北海道大学函館キャンパス
• [Remarks] ホームページ等
  • URL: http://www.geocities.jp/hlaboratory/index.html
• [Remarks]
  • URL: http://www.geocities.jp/hlaboratory/index.html
• [Remarks]
  • URL: http://www.geocities.co.jp/hlaboratory/