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Study on the metabolic pathways for seaweed polysaccharides in abalone

Research Project

Project/Area Number 19380117
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionHokkaido University

Principal Investigator

OJIMA Takao  Hokkaido University, 大学院・水産科学研究院, 教授 (30160865)

Co-Investigator(Kenkyū-buntansha) INOUE Akira  北海道大学, 大学院・水産科学研究院, 准教授 (70396307)
TANAKA Hiroyuki  北海道大学, 大学院・水産科学研究院, 助教 (90241372)
SAWABE Tomoo  北海道大学, 大学院・水産科学研究院, 教授 (30241376)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywordsアワビ / アメフラシ / 腹足類 / 多糖分解酵素 / 海藻多糖 / 代謝 / アルギン酸 / ラミナラン / オリゴ糖 / 糖代謝 / 解凍系 / 海藻 / 多糖 / α-ケト酸 / フコイダン / 解糖系
Research Abstract

Herbivorous marine gastropods like abalone and sea hare possess various polysaccharide-degrading enzymes which can degrade seaweeds' polysaccharides such as alginate, laminaran, mannan, xylane, and cellulose to oligo- and monosaccharides. These saccharides are considered to be metabolized via glycolytic pathway and TCA cycle. In the present study, we isolated several polysaccharide-degrading enzymes, e.g., alginate lyase, laminarinase and mannanase, from abalone and sea hare and investigated their biological roles in the metabolism of seaweeds' polysaccharides in gastropods.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (87 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (29 results) (of which Peer Reviewed: 15 results) Presentation (55 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] cDNA cloning and bacterial expression of an endo-β-1, 4-mannanase, AkMan, from Aplysia kurodai.2011

    • Author(s)
      U.A. Zahura, M.M. Rahman, A. Inoue, H. Tanaka, T. Ojima.
    • Journal Title

      Comp.Biochem.Physiol., part B

    • NAID

      120003169051

    • Related Report
      2010 Final Research Report
  • [Journal Article] Protoplast preparation from Laminaria japonica with recombinant alginate lyase and cellulase.2010

    • Author(s)
      A. Inoue, C. Mashino, T. Kodama, T. Ojima
    • Journal Title

      Mar.Biotech. 13(2)

    • Related Report
      2010 Final Research Report
  • [Journal Article] Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai.2010

    • Author(s)
      M.M. Rahman, A. Inoue, H. Tanaka, T. Ojima
    • Journal Title

      Comp.Biochem.Physiol. 157B

      Pages: 317-25

    • NAID

      120002513622

    • Related Report
      2010 Final Research Report
  • [Journal Article] An endo-β-1,4 mannanase, AkMan, from the common sea hare Aplysia kurodai.2010

    • Author(s)
      U.A. Zahura, M.M. Rahman, A. Inoue, H. Tanaka, T. Ojima
    • Journal Title

      Comp.Biochem.Physiol. 157B

      Pages: 137-143

    • Related Report
      2010 Final Research Report
  • [Journal Article] Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines.2010

    • Author(s)
      F. Okumura, H. Kameda, T. Ojima, S. Hatakeyama
    • Journal Title

      Biochem.Biophys.Res.Commun. 395

      Pages: 352-355

    • NAID

      120002148680

    • Related Report
      2010 Final Research Report
  • [Journal Article] Isolation and characterization of two types of β-1, 3-glucanases from the common sea hare Aplysia kurodai.2010

    • Author(s)
      Y. Kumagai, T. Ojima
    • Journal Title

      Comp.Biochem.Physiol. 155B

      Pages: 138-144

    • NAID

      120001730714

    • Related Report
      2010 Final Research Report
  • [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga2010

    • Author(s)
      C. Nagasato, A. Inoue, M. Mizuno, K. Kanazawa, T. Ojima, K. Okuda, T. Motomrua
    • Journal Title

      Silvetia babingtonii (Fucales, Phaeophyceae), Planta 232

      Pages: 287-298

    • Related Report
      2010 Final Research Report
  • [Journal Article] Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines2010

    • Author(s)
      F.Okumura, H.Kameda, T.Ojima, S.Hatakeyama
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 395 Pages: 352-355

    • NAID

      120002148680

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)2010

    • Author(s)
      C.Nagasato, A.Inoue, M.Mizuno, K.Kanazawa, T.Ojima, Okuda, T.Motomura
    • Journal Title

      Planta

      Volume: 232 Pages: 287-298

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An endo-β-1,4 mannanase, AkMan, from the common sea hare Aplysia kurodai2010

    • Author(s)
      U.A.Zahura, M.M.Rahman, A.Inoue, H.Tanaka, T.Ojima
    • Journal Title

      Comp.Biochem.Physiol.

      Volume: 1157B Pages: 137-143

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai.2010

    • Author(s)
      M.M.Rahman, A.Inoue, H.Tanaka, T.Ojima
    • Journal Title

      Comp.Biochem.Physiol.

      Volume: 157B Pages: 317-325

    • NAID

      120002513622

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of two types of β-1, 3-glucanases from the common sea hare Aplysia Kurodai2010

    • Author(s)
      Y.Kumagai, T.Ojima
    • Journal Title

      Comp.Biochem.Physiol. 155B

      Pages: 138-144

    • NAID

      120001730714

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic properties and cDNA cloning of a β-1, 3-glucanase from the Pacific abalone Haliotis discus hannai.2009

    • Author(s)
      Y. Kumagai, T. Ojima
    • Journal Title

      Comp.Biochem.Physiol. 154B

      Pages: 113-120

    • Related Report
      2010 Final Research Report
  • [Journal Article] Quantification and localization of fucoidan in Laminaria japonica using a novel antibody.2009

    • Author(s)
      M. Mizuno, Y. Nishitani, T. Tanoue, Y. Matoba, T. Ojima, T. Hashimoto, K. Kanazawa
    • Journal Title

      Biosci.Biotech.Biochem. 73

      Pages: 335-338

    • NAID

      10027537283

    • Related Report
      2010 Final Research Report
  • [Journal Article] Comparative study on general properties of alginate lyases from some marine gastropod mollusks.2009

    • Author(s)
      M. Hata, Y. Kumagai, M.M. Rahman, S. Chiba, H. Tanaka, A. Inoue, T. Ojima
    • Journal Title

      Fish.Sci. 75

      Pages: 755-763

    • NAID

      10026550581

    • Related Report
      2010 Final Research Report
  • [Journal Article] Enzymatic properties and cDNA cloning of a β-1,3-glucanase from the Pacific abalone Haliotis discus hannai2009

    • Author(s)
      Y. Kumagai, T. Ojima
    • Journal Title

      Comp.Biochem.Physiol. 154B

      Pages: 113-120

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Comparative study on general properties of alginate lyases from some marine gastropod mollusks2009

    • Author(s)
      M. Hata, Y. Kumagai, M. M. Rahman, S. Chiba, H. Tanaka, A. Inoue, T. Ojima
    • Journal Title

      Fish.Sci. 75

      Pages: 755-763

    • NAID

      10026550581

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Comparative study on general properties of alginate lyases from some marine gastropod mollusks2009

    • Author(s)
      M.Hata, Y.Kumagai, M.M.Rahman, S.Chiba, H.Tanaka, A.Inoue, T.Ojima
    • Journal Title

      Fish.Sci. 75

      Pages: 755-763

    • NAID

      10026550581

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic properties and cDNA cloning of a β-1, 3-glucanase from the Pacific abalone Haliotis discus hannai2009

    • Author(s)
      Y.Kunmagai, T.Ojima
    • Journal Title

      Comp.Biochem.Physiol. 154B

      Pages: 113-120

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis.2008

    • Author(s)
      S. Yamamoto, T. Sahara, D. Sato, K. Kawasaki, S. Ohgiya, A. Inoue, T. Ojima
    • Journal Title

      J.Enzym.Microbiol.Tech. 43

      Pages: 396-402

    • NAID

      120001311788

    • Related Report
      2010 Final Research Report
  • [Journal Article] Preparation of β-1, 3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides.2008

    • Author(s)
      Y. Kumagai, A. Inoue, H. Tanaka, T. Ojima
    • Journal Title

      Fish.Sci. 74

      Pages: 1127-1136

    • NAID

      10021873360

    • Related Report
      2010 Final Research Report
  • [Journal Article] Preparation of protoplasts from Laminaria japonica using native and recombinant abalone alginate lyases.2008

    • Author(s)
      A. Inoue, M. Kagaya, T. Ojima
    • Journal Title

      J.Appl.Phycol. 20

      Pages: 663-640

    • Related Report
      2010 Final Research Report
  • [Journal Article] Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis2008

    • Author(s)
      S. Yamamoto, T. Sahara, D. Sato, K. Kawasaki, S. Ohgiya, A. Inoue, T Ojima
    • Journal Title

      J.Enzym.Microbiol.Tech. 43

      Pages: 396-402

    • NAID

      120001311788

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Preparation of protoplasts from Laminaria japonica using native and recombinant abalone alginate lyases2008

    • Author(s)
      A. Inoue, M. Kagaya, and A T. Ojima
    • Journal Title

      J. Appl. Phycol. 20

      Pages: 663-640

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of β-1,3-glucanase from scallop mid-gut gland drips and its use for production of novelheterooligosaccharides2008

    • Author(s)
      Y. Kumagai, A. Inoue, H. Tanaka, and T. Ojima
    • Journal Title

      Fish. Sci. 74

      Pages: 1127-1136

    • NAID

      10021873360

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis2008

    • Author(s)
      S. Yamamoto, T. Sahara, D. Sato, K. Kawasaki, S. Ohgiya, A. Inoue, and T. Ojima
    • Journal Title

      J. Enzvm. Microbiol. Tech. 43

      Pages: 396-402

    • NAID

      120001311788

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus.2007

    • Author(s)
      Y. Nishida, K. Suzuki, Y. kumagai, H. Tanaka, A. Inoue, T. Ojima
    • Journal Title

      Biochimie 89

      Pages: 1002-1011

    • Related Report
      2010 Final Research Report
  • [Journal Article] Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus.2007

    • Author(s)
      Y. Nishida, K. Suzuki, Y. kumagai, H. Tanaka, A. Inoue, and T. Ojima
    • Journal Title

      Biochimie 89

      Pages: 1002-1011

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of protoplasts from Laminaria japonica using native and recombinant abalone alginate lyases.(DOI:10.1007/s10811-007-9199-y).2007

    • Author(s)
      A.Inoue, M. Kagaya, and T. Ojima
    • Journal Title

      J. Appl. Phycol. E-pub

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] エゾアワビα-グルコシダーゼの一次構造2011

    • Author(s)
      佐藤琢哉・井上晶・田中啓之・尾島孝男
    • Organizer
      平成23年度日本水産学会春季大会(講演要旨集による学会発表)
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] cDNA cloning and bacteria expression of an alginate lyase isozyme from Littorina brevicula2011

    • Author(s)
      M.M. Rahman, A.Inoue, H.Tanaka, T.Ojima
    • Organizer
      平成23年度日本水産学会春季大会(講演要旨集による学会発表)
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Primary structure analysis of Littorina brevicula alginate lyase LbAly352011

    • Author(s)
      L. Wang, M.M. Rahman, H.Tanaka, A. Inoue, T.Ojima
    • Organizer
      平成23年度日本水産学会春季大会(講演要旨集による学会発表)
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビ・アルギン酸リアーゼの作用特性に関するタンパク質工学的研究2011

    • Author(s)
      安部秀昭・井上晶・田中啓之・尾島孝男
    • Organizer
      平成23年度日本水産学会春季大会(講演要旨集による学会発表)
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Mannan-degrading enzymes from Aplysia kurodai2011

    • Author(s)
      U.A. Zahura, M.M. Rahman, A.Inoue, H.Tanaka, T.Ojima
    • Organizer
      平成23年度日本水産学会春季大会(講演要旨集による学会発表)
    • Place of Presentation
      東京海洋大学(東京都品川区
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビα-グルコシダーゼの一次構造2011

    • Author(s)
      佐藤琢哉・井上晶・田中啓之・尾島孝男
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)(震災により口頭発表は中止されたが、講演要旨集の発行により発表したものと取り扱うこととなった)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] cDNA cloning and bacteria expression of an alginate lyase isozyme from Littorina brevicula2011

    • Author(s)
      M.M.Rahman, A.Inoue, H.Tanaka, T.Ojima
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)(震災により口頭発表は中止されたが、講演要旨集の発行により発表したものと取り扱うこととなった)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Primary structure analysis of Littorina brevicula alginate lyase LbAly352011

    • Author(s)
      L.Wang, M.M.Rahman, H.Tanaka, A.Inoue, T.Ojima
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)(震災により口頭発表は中止されたが、講演要旨集の発行により発表したものと取り扱うこととなった)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゾアワビ・アルギン酸リアーゼの作用特性に関するタンパク質工学的研究2011

    • Author(s)
      安部秀昭・井上晶・田中啓之・尾島孝男
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)(震災により口頭発表は中止されたが、講演要旨集の発行により発表したものと取り扱うこととなった)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mannan-degrading enzymes from Aplysia kurodai2011

    • Author(s)
      U.A.Zahura, M.M.Rahman, A.Inoue, H.Tanaka, T.Oiima
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)(震災により口頭発表は中止されたが、講演要旨集の発行により発表したものと取り扱うこととなった)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] エゾアワビ肝膵臓におけるアルギン酸代謝に関する研究2010

    • Author(s)
      木本雄太・熊谷祐也・澤辺智雄・田中啓之・井上晶・尾島孝男
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] cDNA cloning and bacterial expression of Aplysia kurodai alginate lyase2010

    • Author(s)
      Mohammad Matiur Rahman, Akira Inoue, Hiroyuki Tanaka, Takao Ojima
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Primary structure of an endo-β-1,4-mannanase from Aplysia kurodai2010

    • Author(s)
      Umme Afsari Zahura, Mohammad Matiur Rahman, Hiroyuki Tanaka, Akira Inoue, Takao Ojima
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ホタテガイβ-1,3-グルカナーゼの点変異体を用いたサブサイト+1の機能解析2010

    • Author(s)
      熊谷祐也・井上晶・尾島孝男
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビ肝膵臓におけるアルギン酸代謝に関する研究2010

    • Author(s)
      木本雄太, 熊谷祐也, 井上晶, 田中啓之, 澤辺智雄, 尾島孝男
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部、藤沢
    • Year and Date
      2010-03-27
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] エゾアワビ肝膵臓におけるアルギン酸代謝に関する研究2010

    • Author(s)
      木本雄太・熊谷祐也・澤辺智雄・田中啓之・井上晶・尾島孝男
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] cDNA cloning and bacterial expression of Aplysia Kurodai alginate lyase2010

    • Author(s)
      Mohammad Matiur Rahman, Akira Inoue, Hiroyuki Tanaka, Takan Ojima
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Primary structure of an endo-β-1, 4-mannanase from Aplysia kurodai2010

    • Author(s)
      Umme Afsari Zahura, Mohammad Matiur Rahman, Hiroyuki Tanaka, Akira Inoue, Takao Ojima
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタテガイβ-1,3-グルカナーゼの点変異体を用いたサブサイト+1の機能解析2010

    • Author(s)
      熊谷祐也・井上晶・尾島孝男
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日大生物資源科学部
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Alginate lyase from a deep-sea hydrothermal vent bacterium Nitratiruptor sp.2010

    • Author(s)
      A.Inoue, M. Anraku, S. Nakagawa, T.Sawabe, T.Ojima
    • Organizer
      The 9th International Marine Biotechnology Conference
    • Place of Presentation
      Huanghai Hotel, Qingdao, China
    • Related Report
      2010 Final Research Report
  • [Presentation] Alginate lyase from a deep-sea hydrothermal vent bacterium Nitratiruptor sp.2010

    • Author(s)
      A.Inoue, M.Anraku, S.Nakagawa, T.Sawabe, T.Oiima
    • Organizer
      The 9th International Marine Biotechnology Conference
    • Place of Presentation
      Huanghai Hotel, Qingdao, China.
    • Related Report
      2010 Annual Research Report
  • [Presentation] エゾアワビ肝膵臓に含まれるアルギン酸分解物由来α-ケト酸の転換因子2009

    • Author(s)
      木本雄太、熊谷祐也、澤辺智雄、尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター(盛岡市)
    • Year and Date
      2009-10-01
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビ・エキソ型アミラーゼ様酵素の単離と性状解析2009

    • Author(s)
      佐藤琢哉・熊谷裕也・井上晶・田中啓之・尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター(盛岡市)
    • Year and Date
      2009-10-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Purification and characterization of an endo-β-1,4-mannanase from the common sea hare Aplysia kurodai2009

    • Author(s)
      Umme Afsari Zahura, Mohammad Matiur Rahman, Yuya Kumagai, Akira Inoue, Takao Ojima
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター(盛岡市)
    • Year and Date
      2009-10-01
    • Related Report
      2010 Final Research Report
  • [Presentation] ホタテガイβ-1,3-グルカナーゼPyLam38の点変異体を用いた機能解析2009

    • Author(s)
      熊谷祐也・井上晶・尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター(盛岡市)
    • Year and Date
      2009-10-01
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビ肝膵臓に含まれるアルギン酸分解物由来α-ケト酸の転換因子2009

    • Author(s)
      木本雄太、熊谷祐也、澤辺智雄、尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] エゾアワビ・エキソ型アミラーゼ様酵素の単離と性状解析2009

    • Author(s)
      佐藤琢哉・熊谷裕也・井上晶・田中啓之・尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Purification and characterization of an endo-β-1, 4-mannanase from the common sea hare Aplysia Kurodai2009

    • Author(s)
      Umme Afsari Zabura, Mohammad Matiur Rahman, YuYa Kumagai, Akira Inoue, Takao Ojima
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタテガイβ-1,3-グルカナーゼPyLam38の点変異体を用いた機能解析2009

    • Author(s)
      熊谷祐也・井上晶・尾島孝男
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報センター
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Purification and properties of alginate lyase from Aplysia kurodai2008

    • Author(s)
      Mohammad Matiur Rahman, Takao Ojima
    • Organizer
      The 7th International Symposium. "Innovative Marine Life Science for Three Es, Edible, Enviroument and Education, in 21st Century
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Enzymatic properties of laminarinase from abalone, Haliotis discus hannai2008

    • Author(s)
      Yuya Kumagai, Akira Inoue, Takao Ojima
    • Organizer
      The 7th International Symposium. "Innovative Marine Life Science for Three Es, Edible, Enviroument and Education, in 21st Century.
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Isolation of protoplasts from Laminaria japonica utilizing recombinant cellulase and alginate lyase2008

    • Author(s)
      Akira Inoue, Chieko Mashino, Teina Kodama, Takao Ojima
    • Organizer
      The 7th International Symposium "Innovative Marine Life Science for Three Es, Edible, Enviroument and Education, in 21st Century
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Purification and properties of alginate lyase from Aplysia kurodai2008

    • Author(s)
      Mohammad Matiur Rahman and Takao Ojima
    • Organizer
      The 7^<th> International Symposium "Innovative Marine Life Science for Three Es, Edible, Enviroument and Education, in 21^<st> Century
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enzymatic properties of laminarinase from abalone, Haliotis discus hannai2008

    • Author(s)
      Yuya Kumagai, Akira Inoue. and Takao Oiima
    • Organizer
      The 7^<th> International Symposium "Innovative Marine Life Science for Three Es, Edible, Enviroument and Education, in 21^<st> Century
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Isolation of protoplasts from Laminaria japonicautilizing recombinant cellulase and alginate lyase2008

    • Author(s)
      Akira Inoue, Chieko Mashino, Teina Kodama, and Takao Ojima
    • Organizer
      The 7^<th> International Symposium "Innovative Marine Life Science for Three Es, Edible, Enviroument and, Education, in 21^<st> Century
    • Place of Presentation
      Conference Hall, Hokkaido University, Sapporo, Japan
    • Year and Date
      2008-11-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Purification and cDNA cloning of a β-1,3-glucanase from the pacific abalone, Haliotis discus hannai2008

    • Author(s)
      Yuya Kumagai, Takao Ojima
    • Organizer
      The 8th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Bexco, Busan, Korea
    • Year and Date
      2008-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Purification and cDNA cloning of a b-1,3-glucanase from the pacific abalone, Haliotis discus hannai2008

    • Author(s)
      Yuya Kumagai and Takao Ojima
    • Organizer
      The 8^<th> Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Bexco, Busan, Korea
    • Year and Date
      2008-11-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Bioconversion of algal polysaccharides by polysaccharide-degrading enzymes from marine organisms2008

    • Author(s)
      尾島孝男
    • Organizer
      The 5th World Fisheries Congress.
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Preparation of β-1,3-glucanase from scallop mid-gut gland and its utilization for production of novel heterooligosaccharides2008

    • Author(s)
      Yuya Kumagai, Takao Ojima
    • Organizer
      The 5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Comparative study on alginate lyases from Aplysia krodai and Haliotis discus2008

    • Author(s)
      Mohammad Matiur Rahman, Takao Ojima
    • Organizer
      The 5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Bioconversjon of algal polysaccharides by polysaccharide-degrading enzymes from marine organisms2008

    • Author(s)
      Takao Ojima
    • Organizer
      The 5^<th> World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Preparation of β-1,3-glucanase from scallop mid-gut gland and its utilization for production of novel heterooligosaccharides2008

    • Author(s)
      Yuya Kumagai and Takao Ojima
    • Organizer
      The 5^<th> World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Comparative study on alginate lyases from Aplysia krodai and Haliotis discus2008

    • Author(s)
      Mohammad Matiur Rahman and Takao Ojima.
    • Organizer
      The 5^<th> World Fisheries Congress
    • Place of Presentation
      Pacifico Yokdhama, Kanagawa, Japan
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Utilization of a β-1,3-glucanase from scallop visceral waste for the production of novel heterooligosaccharides2008

    • Author(s)
      Takao Ojima, Yuya Kumagai
    • Organizer
      The 4th International Symposium on the Marine Biotechnology and Advanced Materials.
    • Place of Presentation
      Kangnung National University, Gangneung, Korea
    • Year and Date
      2008-06-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Utilization of a β-1,3-glucanase from scallop visceral waste for the production of novel heterooligosaccharide2008

    • Author(s)
      Takao Ojima and Yuya Kumagai
    • Organizer
      The 4^<th> International Symposium on the Marine Biotechnology and Advanced Mate rials
    • Place of Presentation
      Kangnung National Unive rsity, Gangneung, Korea
    • Year and Date
      2008-06-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] エゾアワビ・アルギン酸リアーゼによるアルギン酸分解機構の解析2008

    • Author(s)
      長畑雄也、木本雄太、澤辺智雄、井上晶、尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Characterization of alginate lyase from Aplysia kurodai2008

    • Author(s)
      Md. Matiur Rahman, Takao Ojima
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビβ-1,3-グルカナーゼの単離と性状解析2008

    • Author(s)
      熊谷祐也、井上晶、尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] エゾアワビα-グルカン分解酵素の基本性状2008

    • Author(s)
      加藤史顕、熊谷雄也、井上晶、尾島孝男
    • Place of Presentation
      東海大学海洋学部(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 組み換え多糖類分解酵素を用いたマコンブ・プロトプラストの作出2008

    • Author(s)
      井上晶・児玉貞奈・尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] エゾアワビ・アルギン酸リアーゼによるアルギン酸分解機構の解析2008

    • Author(s)
      長畑雄也, 熊谷祐也, 井上品, 田中啓之, 澤辺智雄, 尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部 (清水)
    • Year and Date
      2008-03-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] エゾアワビ・アルギン酸リアーゼによるアルギン酸分解機構の解析2008

    • Author(s)
      尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] エゾアワビα-グルカン分解酵素の基本性状2008

    • Author(s)
      尾島孝男
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] エゾアワビβ-1,3-グルカナーゼの単離と性状解析2008

    • Author(s)
      熊谷 祐也
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Utilization of a β-1,3-glucanase from mollusks for the production of novel heterooligosaccharides2008

    • Author(s)
      Takao Ojima, Yuya Kumagai
    • Organizer
      The 4th international symposium on the marine biotechnology and advanced materials
    • Place of Presentation
      Kangnung National University, Korea
    • Related Report
      2009 Self-evaluation Report
  • [Remarks] ホームページ等

    • URL

      http://mmb.fish.hokudai.ac.jp/MMB/TOP.html

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] アメフラシ由来キシログルカナーゼ2010

    • Inventor(s)
      尾島孝男
    • Industrial Property Rights Holder
      国立大学法人北海道大学三菱レイヨン株式会社
    • Industrial Property Number
      2010-033922
    • Filing Date
      2010-02-18
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] アメフラシ由来マンナナーゼ2009

    • Inventor(s)
      尾島孝男
    • Industrial Property Rights Holder
      国立大学法人北海道大学三菱レイヨン株式会社
    • Industrial Property Number
      2009-214412
    • Filing Date
      2009-09-16
    • Related Report
      2010 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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