Bioactive compounds that regulate downstream migration of the sockeye salmon Oncorhynchus nerka

• Project/Area Number: 19380118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Utsunomiya University
• Principal Investigator: YANAGISAWA Tadashi Utsunomiya University, 農学部, 教授 (90134262)
• Co-Investigator(Kenkyū-buntansha): AZUMA Teruo 独立行政法人水産総合研究センター, 中央水産研究所, 室長 (90360768) ; IIGO Masayuki 宇都宮大学, 農学部, 准教授 (10232109) ; NIHEI KenーIchi 宇都宮大学, 農学部, 准教授 (10307209)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: サケ科魚類 / 回遊 / 降河行動 / 天然生物活性物質 / ホルモン / 脳 / 下垂体 / 甲状腺

Research Abstract

Regulatory mechanisms by bioactive compounds of down-stream migration in the sockeye salmon (Oncorhynchus nerka) were investigated. Molecules involved in the regulation of the hypothalamo-pituitary-thyroid axis were selected as targets. cDNAs encoding thyrotropin-releaseing hormone (TRHa and TRHb), 4 subtypes of TRH receptor (TRHR1, TRHR2a, TRHR2b, and TRHR3), and deiodinase type II were cloned and the sites of expression were demonstrated by RT-PCR. These results indicate that the hypothalamo-pituitary-thyroid axis plays important roles in the regulation of down-stream migration of the sockeye salmon.

Research Products

• [Journal Article] Lack of circadian regulation of in vitro melatonin release from the pineal organ of salmonid teleosts. (2007)
  • Author(s): Iigo M, Abe T, Kambayashi S, Oikawa K, Masuda T, Mizusawa K, Kitamura S, Azuma T, Takagi Y, Aida K, Yanagisawa T
  • Journal Title: General and Comparative Endocrinology 154 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Lack of circadian regulation of in vitro melatonin release from the pineal organ of salmonid teleosts (2007)
  • Author(s): Iigo M, Abe T, Kambayashi S, Oikawa K, Masuda T, Mizusawa K, Kitamura S, Azuma T, Takagi Y, Aida K, Yanagisawa T
  • Journal Title: General and Comparative Endocrinology 154 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Molecular cloning, gene structure, molecular evolution and expression analyses of thyrotropin-releasing hormone receptors from medaka (Oryzias latipes).
  • Author(s): Mekuchi M, Saito Y, Aoki Y, Masuda T, Iigo M, Yanagisawa T
  • Journal Title: General and Comparative Endocrinology in press
  • Peer Reviewed
• [Presentation] 魚類における本能的脳機能の機能的ゲノミクス解析 (2010)
  • Author(s): 飯郷雅之
  • Organizer: 第9回シンポジウム「水生動物の行動と神経系」
  • Place of Presentation: 大阪府大阪市
• [Presentation] ベニザケGPR54のcDNAクローニング (2009)
  • Author(s): 角田麻衣, 斉藤悠一, 東照雄, 柳沢忠, 飯郷雅之
  • Organizer: 平成21年度日本水産学会大会
  • Place of Presentation: 東京
• [Presentation] 魚類のKiSS-2ペプチド:前駆体cDNAのクローニングと成熟ペプチドの精製 (2009)
  • Author(s): 大瀧直仁, 恒川賢太, 大杉知裕, 飯郷雅之, 天野勝文, 筒井和義
  • Organizer: 第34回日本比較内分泌学会大会日本比較生理生化学会第31回大会合同大会CompBiol2009
  • Place of Presentation: 大阪府大阪市
• [Presentation] べ二ザケGPR54のcDNAクローニング (2009)
  • Author(s): 角田麻衣, 斉藤悠一, 東照雄, 柳沢忠, 飯郷雅之
  • Organizer: 平成21年度日本水産学会大会
• [Presentation] サケ科魚類における甲状腺刺激ホルモン放出ホルモン受容体のcDNAクローニングと発現解析 (2008)
  • Author(s): 齋藤悠一, 青木康洋, 東照雄, 飯郷雅之, 柳沢忠
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] Pharmacological and molecular characterization of melatonin receptors in ayu (Plecoglossus altivelis), an osmerid teleost. (2008)
  • Author(s): 斉藤悠一, 小林宣章, Amiya N, Yasuo S, 吉村崇, 天野勝文, 柳沢忠, 飯郷雅之
  • Organizer: Japan Taiwan Bilateral Science & Technology Symposium
  • Place of Presentation: Utsunomiya.
• [Presentation] Molecular cloning of parapinopsin cDNA from the pineal organ of the masu salmon (Oncorhynchus masou). (2008)
  • Author(s): Kimijima M, Uesaka M, Takahashi D, 柳沢忠, 飯郷雅之
  • Organizer: Japan Taiwan Bilateral Science & Technology Symposium
  • Place of Presentation: Utsunomiya.
• [Presentation] Molecular cloning and expression analysis of GPR54 in medaka (Oryzias latipes) and two salmonids, sockeye salmon (Oncorhynchus nerka) and masu salmon (O.masou). (2008)
  • Author(s): 角田麻衣, Kasahara K, 斉藤悠一, 阿部朋孝, 柳沢忠, 飯郷雅之
  • Organizer: Japan Taiwan Bilateral Science & Technology Symposium
  • Place of Presentation: Utsunomiya.
• [Presentation] Deep-brain photoreceptive molecules in fish (2008)
  • Author(s): Takahashi D, Uesaka M, Fujita S, 東照雄, 柳沢忠, 飯郷雅之
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama.
• [Presentation] Molecular cloning of the second prepro-thyrotropin-releasing hormone cDNA in the sockeye salmon (Oncorhynchus nerka) (2008)
  • Author(s): 斉藤悠一, 青木康洋, 東照雄, 飯郷雅之, 柳沢忠
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama.
• [Presentation] 魚類におけるプレプロ甲状腺刺激ホルモン放出ホルモンの分子進化 (2008)
  • Author(s): 青木康洋, 飯郷雅之, 柳沢忠
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 静岡
• [Presentation] Molecular cloning of the second prepro-thyrotropin-releasing hormone cDNA in the sockeye salmon(Oncorhynchus nerka) (2008)
  • Author(s): Saito Y, Aoki Y, Azuma T, Iigo M, Yanisawa T
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: 神奈川県横浜市
• [Presentation] Molecular cloning and expression analysis of GPR54 in medaka (Oryzias latipes) and two salmonids, sockeye salmon (Oncorhynchus nerka) and masu salmon (0.massou) (2008)
  • Author(s): Tsunoda M Kasahara K, Saito Y, Abe T ,Yanagisawa T, ligo M
  • Organizer: Japan Taiwan Bilateral Science&Technology Symposium
  • Place of Presentation: 栃木県宇都宮市
• [Presentation] サケ科魚類における甲状腺刺激ホルモン放出ホルモン受容体のcDNAクローニングと発現解析 (2008)
  • Author(s): 齋藤悠一, 青木康洋, 東照雄, 柳沢忠, 飯郷雅之
  • Organizer: BMB2008
  • Place of Presentation: 兵庫県神戸市
• [Presentation] 日長・運動・高温処理がサクラマス雄早熟化に与える影響 (2007)
  • Author(s): 東照雄, 飯郷雅之, 柳沢忠, 天野勝文, 山森邦夫, 朝比奈潔
  • Organizer: 第6回日本水産増殖学会大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-18
• [Presentation] 甲状腺刺激ホルモン放出ホルモン受容体の分子進化 (2007)
  • Author(s): 斎藤悠一, 小林宣章, 木村真, 馬久地みゆき, 青木康洋, 東照雄, 飯郷雅之, 柳沢忠
  • Organizer: 第32回日本比較内分泌学会大会
• [Presentation] サケ科魚類松果体からのメラトニン分泌の生物時計による制御は進化のどの段階で消失したのか? (2007)
  • Author(s): 阿部朋孝, 増田智浩, 水澤寛太, 北村章二, 東照雄, 都木靖彰, 柳沢忠, 飯郷雅之
  • Organizer: 第32回日本比較内分泌学会大会
• [Presentation] プレプロ甲状腺刺激ホルモン放出ホルモンの分子進化 (2007)
  • Author(s): 青木康洋, 斎藤悠一, 朴民根, 西源二郎, 吉村崇, 飯郷雅之, 柳沢忠
  • Organizer: 第32回日本比較内分泌学会大会
• [Presentation] Circadian regulation of rhythmic melatonin release from the teleostean pineal organ : a comparative study. (2007)
  • Author(s): 飯郷雅之, 阿部朋孝, Oikawa K, Fujita S, Endo M, 柳沢忠, 西源二郎
  • Organizer: 72nd Cold Spring Harbor Symposium : Clocks & Rhythms
  • Place of Presentation: Cold Spring Harbor, NY, USA.
• [Book] 光周性の分子生物学(海老原史樹文, 井澤毅編 魚類の光周性) (2009)
  • Author(s): 飯郷雅之
  • Publisher: シュプリンガーフェアラーク東京
• [Book] 光周性の分子生物学 (2009)
  • Author(s): 飯郷雅之(海老原史樹文,井澤毅編)
  • Total Pages: 199
  • Publisher: シュプリンガーフェアラーク東京
• [Book] 時間生物学事典(石田直理雄, 本間研一編 第3部生物リズムの研究法37.光パルス) (2008)
  • Author(s): 飯郷雅之
  • Publisher: 朝倉書店
• [Book] 時間生物学事典(石田直理雄, 本間研一編 第3部生物リズムの研究法37.温度パルス) (2008)
  • Author(s): 飯郷雅之
  • Publisher: 朝倉書店
• [Book] 時間生物学事典(石田直理雄, 本間研一編 第4部生物時計54.松果体) (2008)
  • Author(s): 飯郷雅之
  • Publisher: 朝倉書店
• [Book] ホルモンハンドブック新訂eBook版(日本比較内分泌学会編 インドールアミン類(概説)) (2007)
  • Author(s): 飯郷雅之
  • Publisher: 南江堂
• [Book] ホルモンハンドブック新訂eBook版(日本比較内分泌学会編 セロトニン) (2007)
  • Author(s): 飯郷雅之
  • Publisher: 南江堂
• [Book] ホルモンハンドブック新訂eBook版(日本比較内分泌学会編 メラトニン) (2007)
  • Author(s): 飯郷雅之
  • Publisher: 南江堂
• [Remarks] ホームページ等
  • URL: http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/chemj/jorganic/jorganic.html