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Exhaustive studies on filament forming proteins of molluskan muscle

Research Project

Project/Area Number 19380119
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

OCHIAI Yoshihiro  The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (50160891)

Co-Investigator(Kenkyū-buntansha) WATABE Shugo  東京大学, 大学院・農学生命科学研究科, 教授 (40111489)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Keywords軟体類 / 筋肉 / タンパク質 / フィラメント / 構造 / 軟体動物 / フィラメント形成 / 一次構造 / 立体構造 / ミオシン / フィラメント形成能 / 構造機能解析
Research Abstract

Muscular filament-forming proteins, namely, myosin heavy chain, actin and tropomyosin from four species of mollusks (cephalopods) have been investigated. As for tropomyosin, those from other species were examined for their structural stability. Primary structure analysis based on cDNA cloning, phylogenetic analysis based on amino acid sequence, and functional and structural analysis based on tertiary structure simulation have revealed the specificity and uniqueness of those contractile proteins. The results obtained suggested that mollusks, especially cephalopods, have evolved protein related to muscle contraction to compensate for their specialized locomotion activities. On the other hand, the properties of myosin heavy chain, etc. would give important information for the effective utilization and processing of mollusks.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2010 2009 2008 2007 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (21 results)

  • [Journal Article] Thermostability of striated and smooth adductor muscle tropomyosins from Yesso scallop2010

    • Author(s)
      H. Ozawa, S. Watabe, Y. Ochiai
    • Journal Title

      Mizuhopecten yessoensis. Journal of Biochemistry Vol.147

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Strategies of aquatic animals for adaptation to ambient temperatures as estimated from the thermal stability of myofibrillar proteins2009

    • Author(s)
      Y. Ochiai, H. Ozawa, S. Watabe
    • Journal Title

      Journal of Marine and Fisheries Postharvest and Biotechnology Vol.4(Special Ed.)

      Pages: 9-15

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of molluscan muscle based on the properties of major myofibrillar protein components.2009

    • Author(s)
      Y. Ochiai, G.F. Wang, H. Ozawa, S. Watabe
    • Journal Title

      Asian Fisheries Science Vol.22(2)

      Pages: 403-414

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermal stability of the synthetic peptides with the sequence of fish fast skeletal muscle tropomyosin

    • Author(s)
      H.Ozawa, S. Watabe, Y. Ochiai
    • Journal Title

      Fisheries Science 75(印刷中(掲載確定))

    • NAID

      10024979732

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Primary structure and phylogenetic analysis of tropomyosin from spear squid Loligo bleekeri arm muscle2010

    • Author(s)
      王国峰、渡部終五、落合芳博
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 軟体動物筋肉アクチンの構造特性2010

    • Author(s)
      落合芳博、王国峰、渡部終五
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      中央大学(東京)
    • Year and Date
      2010-01-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Primary structure and phylogenetic analysis of tropomyosin from spear squid Loligo bleekeri arm muscle.2010

    • Author(s)
      G.F. Wang, S. Watabe, Y. Ochiai
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of myosin-actin interface of molluskan striated muscle2009

    • Author(s)
      Y.Ochiai, G.F.Wang, S.Watabe
    • Organizer
      The 23rd Symposium of The Protein Society
    • Place of Presentation
      ボストン(米国)
    • Year and Date
      2009-07-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 軟体動物筋肉アクチンの構造上の特徴2009

    • Author(s)
      落合芳博、王国峰、渡部終五
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      熊本全日空ホテル
    • Year and Date
      2009-05-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Strategies of aquatic animals for adaptation to ambient temperatures as estimated from the thermal stability of myofibrillar proteins2009

    • Author(s)
      Y.Ochiai, H.Ozawa, S.Watabe
    • Organizer
      World Ocean Congress 2009
    • Place of Presentation
      マナド(インドネシア)
    • Year and Date
      2009-05-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] 軟体動物筋肉アクチンの構造上の特徴.2009

    • Author(s)
      落合芳博, 王国峰, 渡部終五
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of myosin-actin interface of molluskan striated muscles.2009

    • Author(s)
      Y. Ochiai, G.F. Wang, S. Watabe
    • Organizer
      The 23rd Symposium of The Protein Society
    • Place of Presentation
      Boston
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of skip residues in myosin rod as revealed by synthetic peptides2008

    • Author(s)
      Y. Ochiai, G. F. Wang, S. Watabe
    • Organizer
      生物物理学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Evolution of muscle myosins as characterized by the sequence diversity of subfragment-1 regions Cairns ConventionCenterオーストラリアEvolution of muscle myosins as characterized by the sequence diversity of subfragment-1 regions2008

    • Author(s)
      Y. Ochiai, G. F. Wang, S. Watabe
    • Organizer
      Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      BEXCO,Busan韓国
    • Year and Date
      2008-11-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Thermodynamic properties of short synthetic peptides with the sequences of fish skeletal muscle tropomyosin2008

    • Author(s)
      H. Ozawa, S. Watabe, Y. Ochiai
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-10-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of cephalopod muscle myosin heavy chains2008

    • Author(s)
      G.F.Wang, Y. Ochiai, S. Watabe
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      パシフイコ横浜
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] Characterization of subfragment-2 regions of myosins from invertebrate and vertebrate striated muscles2008

    • Author(s)
      Y. Ochiai, G. F. Wang, S, Watabe
    • Organizer
      Pacific Rim International Conference on Protein Science
    • Place of Presentation
      Cairns ConventionCenterオーストラリア
    • Year and Date
      2008-06-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] cDNA cloning and deduced primary structure of common octopus Octopus vulgaris muscle myosin heavy chain2008

    • Author(s)
      Guofeng Wang, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      日本水産学会森季大会
    • Place of Presentation
      清水(東海大学海洋学部)
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Evolution of muscle myosins as characterized by the sequence diversity of subfragment-1 regions.2008

    • Author(s)
      Y. Ochiai, G.F. Wang, S. Watabe
    • Organizer
      Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Busan
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of skip residues in myosin rod as revealed by synthetic peptides.2008

    • Author(s)
      Y. Ochiai, G.F. Wang, S. Watabe
    • Organizer
      生物物理学会年会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] cDNA cloning and complete primary structures of myosin heavy chains from spear squid and cuttlefish2007

    • Author(s)
      Guofeng Wang, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      コーチン(インド)
    • Year and Date
      2007-11-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of decapodiformes myosin heaVy chains:Structure,fUnction and evolution2007

    • Author(s)
      Yoshihiro Ochiai, Guofeng Wang, Yo'suke Ono, . Shugo Watabe
    • Organizer
      International Conference of Compurative and Physiology
    • Place of Presentation
      サルバドル(ブラジル)
    • Year and Date
      2007-08-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of decapodiformes myosin heavy chains:Structure,fUnction and evolution2007

    • Author(s)
      Yoshihiro Ochiai, Guofeng Wang, Yosuke Ono, Shugo Watabe
    • Organizer
      8th Asian Fisheries Forum
    • Place of Presentation
      サルバドル(ブラジル)
    • Year and Date
      2007-08-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of decapodiformes myosin heavy chains: Structure, function and evolution International Conference of Comparative Biochemistry and Physiology2007

    • Author(s)
      Y. Ochiai, G.F. Wang, Y. Ono, S. Watabe
    • Organizer
      サルバドル
    • Place of Presentation
      ブラジル
    • Related Report
      2009 Final Research Report
  • [Presentation] cDNA cloning and complete primary structures of myosin heavy chains from spear squid and cuttlefish.2007

    • Author(s)
      G.F. Wang, S. Watabe, Y. Ochiai
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      函館
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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