Library constructionand biological activities of high mannose N-glycan-specific algal lectins
Project/Area Number |
19380122
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Fisheries chemistry
|
Research Institution | Hiroshima University |
Principal Investigator |
HORI Kanji Hiroshima University, 大学院・生物圏科学研究科, 教授 (50116662)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
|
Keywords | レクチン / 藻類 / 高マンノース型糖鎖 / 生物活性 / 海藻 / 遺伝子 / 糖鎖結合特異性 / 分子構造 / 藍藻 / 遺伝子クローニング / 組換え体 / 糖鎖認識 / 抗HIV |
Research Abstract |
We succeeded in the cDNA cloning and preparations of active recombinants of high mannose N-glycan-specific algal lectins thatcan be grouped into three types (Type I~III) based on the recognition moiety within the branched oligomannosides of the N-glycans. Furthermore,we newly isolated and characterized the lectins, which belong to type I, from the three cultivated species of edible seaweeds. We found that the type I~III lectins had the anti-HIV activity and inhibitory activity against the growth of the cultured cells which are derived from human colonic cancer. These lectins were also useful to separate easily the high mannose N-glyans to each other from their mixture.
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Report
(4 results)
Research Products
(28 results)