Molecular breedling for cold stress tolerant forage grasses

• Project/Area Number: 19380148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Hokkaido University
• Principal Investigator: YAMADA Toshihiko Hokkaido University, 北方生物圏フィールド科学センター, 教授 (70343952)
• Co-Investigator(Kenkyū-buntansha): 金澤 章 北海道大学, 大学院・農学研究院, 准教授 (30281794) ; 田村 健一 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 研究員 (10414749)
• Co-Investigator(Renkei-kenkyūsha): KANAZAWA Akira 北海道大学, 大学院・農学研究院, 准教授 (30281794) ; TAMURA Kenichi 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 研究員 (10414749)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 草地利用 / 飼料作物育種 / 遺伝子導入 / 低温耐性 / フルクタン合成酵素遺伝子 / カルス培養 / CBF遺伝子 / 組織培養技術 / フルクタン

Research Abstract

Rapid and efficient in vitro culture system and novel methods for particle bombardment and Agrobacterium-mediated transformation using mature embryos as explants have been established in zoysiagrass (Zoysia japonica). Fructsyltransferase gene from timothy (PpFT1) involving a synthesis of highly polymerized fructan contributed to increase freezing tolerance by accumulation of fructan and glucose in cold acclimated plants. CBF gene (LpCBFIVa) from perennial ryegrass functioned with an increase of freezing tolerance with delaying flower time.

Research Products

• [Journal Article] Molecular mechanisms underlying frost tolerance in perennial grasses adapted to cold climates. (2011)
  • Author(s): S.R.Sandve、A.Kosmala、H.Rudi、S.Fjellheim、M.Rapacz、T.Yamada、O.A.Rognli
  • Journal Title: Plant Science 180巻 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying frost tolerance in perennial grasses a dapted to cold climates (2011)
  • Author(s): S.R.Sandve, A.Kosmala, H.Rudi, S.Fjellheim, M.Rapacz, T.Yamada, O.A.Rognli
  • Journal Title: Plant Science Volume: 180 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.) (2010)
  • Author(s): X.Wang、Y.Hoshino、T.Yamada
  • Journal Title: Grassland Science 56巻 Pages: 198-204
  • NAID: 10027882516
  • Peer Reviewed
• [Journal Article] Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysiajaponica Steud.) (2010)
  • Author(s): X.Wang, Y.Hoshino, T.Yamada
  • Journal Title: Grassland Science Volume: 56 Pages: 198-204
  • NAID: 10027882516
  • Peer Reviewed
• [Journal Article] Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass(Zoysia japonica Steud.) (2010)
  • Author(s): X.Wang, Y.Hoshino, T.Yamada
  • Journal Title: Grassland Science (印刷中)
  • NAID: 10027882516
  • Peer Reviewed
• [Journal Article] Rapid in vitro culture and Agrobacterium-mediated transformation system in zoysiagrass (Zoysia japonica Steud.) (2009)
  • Author(s): X.Wang、F.J.Kong、Y.Hoshino、T.Yamada、T.Yamada
  • Journal Title: Proceedings of Joint 14th Austrasian Plant Breeding Conference and 11th SABRAO Congress CD版
  • Peer Reviewed
• [Journal Article] Transformation of androgenic-derived Festulolium plants (Lolium perenne L. x Festuca pratensis Huds.) by Agrobacterium tumefaciens (2009)
  • Author(s): Y.-D.Guo、H.Hisano、Y.Shimamoto、T.Yamada
  • Journal Title: Plant Cell Tissue Organ Culture 96巻 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Molecular breeding to improve tolerance to abiotic stress (2009)
  • Author(s): T.Yamada
  • Journal Title: Proceedings on the 1st International Congress on Global Climate Changes and Agriculture、Namik Kemal University, Tekirdag、Turkey Pages: 135-149
• [Journal Article] Rapid in vitro culture and Agrobacterium-mediated transformation system in zoysiagrass (Zoysia japonica Steud.) (2009)
  • Author(s): X. Wang, F. J. Kong, Y. Hoshino, T. Yamada, T. Yamada
  • Journal Title: Proceedings of Joint 14th Austrasian Plant Breeding Conference and 11th SABRAO Congress CD版ページ数無
• [Journal Article] Transformation of androgenic-derived Festulolium plants (Lolium perenne L. x Festuca pratensis Huds.) by Agrobacterium tumefaciens (2009)
  • Author(s): Y. -D. Guo, H. Hisano, Y. Shimamoto, T. Yamada
  • Journal Title: Plant Cell Tissue Organ Culture 96 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass (2009)
  • Author(s): H. Hisano, A. Kanazawa, M. Yoshida, M. O. Humphreys, M. Iizuka, K. Kitamura, T. Yamada
  • Journal Title: New Phytologist 178 Pages: 766-780
  • Peer Reviewed
• [Journal Article] Rapid in vitro culture and Agrobacterium-mediated transformation system in zoysiagrass(Zoysia japonica Steud.). (2009)
  • Author(s): X.Wang, F.J.Kong., Y.Hoshino, T.Yamada, T.Yamada
  • Journal Title: Proceedings of Joint 14th Austrasian Plant Breeding Conference and 11th SABRAO Congress (CD版のためページ数無)
  • Peer Reviewed
• [Journal Article] Transformation of androgenic-derived Festulolium plants (Latium perenne L. x Festuca pratensis Huds.) by Azrobacterium tumefaciens (2009)
  • Author(s): Y. -D. Guo, H. Hisano, Y. Shimamoto, T. Yamada
  • Journal Title: Plant Cell Tissue Organ Culture 96 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass (2008)
  • Author(s): H.Hisano、A.Kanazawa、M.Yoshida、M.O.Humphreys、M.Iizuka、K.Kitamura、T.Yamada
  • Journal Title: New Phytologist 178巻 Pages: 766-780
  • Peer Reviewed
• [Journal Article] Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass (2008)
  • Author(s): H. Hisano, A. Kanazawa, M. Yoshida, M. O. Humphreys, M. Iizuka, K. Kitamura, T. Yamada
  • Journal Title: New Phytologist. (印刷中)
  • Peer Reviewed
• [Journal Article] Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)
  • Author(s): X. Wang, Y. Hoshino, T. Yamada
  • Journal Title: Grassland Science (印刷中)
  • NAID: 10027882516
  • Peer Reviewed
• [Presentation] Genetic improvement through a transgenic approach in Miscanthus ssp., a new bioenergy crop (2010)
  • Author(s): T.Yamada, X.Wang, A.Kanazawa, T.Yamada, Y.Hoshino, N.Ukaji
  • Organizer: ASA-CSSA-SSSA 2010 International Annual Meeting
  • Place of Presentation: アメリカ合衆国ロングビーチ
  • Year and Date: 2010-11-03
• [Presentation] Molecular breeding to improve tolerance to abiotic stress in forage grasses(plenary lecture) (2010)
  • Author(s): T.Yamada, K.Tamura
  • Organizer: 6th International Symposium on the Molecular Breeding of Forage and Turf
  • Place of Presentation: アルゼンチン、ブエノスアイレス
  • Year and Date: 2010-03-15
• [Presentation] Genetic improvement through a transgenic approach in Miscanthus ssp., a new bioenergy crop (2010)
  • Author(s): T.Yamada、X.Wang、A.Kanazawa、T.Yamada、Y.Hoshino、N.Ukaji
  • Organizer: ASA-CSSA-SSSA 2010 International Annual Meeting
  • Place of Presentation: アメリカ合衆国、ロングビーチ市
• [Presentation] Molecular breeding to improve tolerance to abiotic stress in forage grasses (2010)
  • Author(s): 山田敏彦、田村健一
  • Organizer: 6th International Symposium on the Molecular Breeding of Forage and Turf
  • Place of Presentation: アルゼンチン、ブエノスアイレス市(招待講演)
• [Presentation] Molecular breeding to improve tolerance to abiotic stress in forage grasses (plenary lecture) 6th International Symposium on the Molecular (2010)
  • Author(s): T. Yamada, K. Tamura
  • Organizer: Breeding of Forage and Turf
  • Place of Presentation: アルゼンチン, ブエノスアイレス市
• [Presentation] Rapid in vitro callus induction and regeneration of zoysiagrass (Zoysia japonica Steud.) and miscanthus (Miscanthus sinensis Anderss.) (2009)
  • Author(s): X.Wang、Y.Hoshino、T.Yamada
  • Organizer: 第65回日本草地学会
  • Place of Presentation: 藤沢市(日本大学)
• [Presentation] Rapid in vitrocallus induction and regeneration of zoysiagrass (Zoysia japonica Steud.) and miscanthus (Miscanthus sinensis Anderss.) (2009)
  • Author(s): X. Wang, Y. Hoshino, T. Yamada
  • Organizer: 第65回日本草地学会
  • Place of Presentation: 藤沢市 (日本大学)
• [Presentation] Rapid in vitro callus induction and regeneration of zoysiagrass (Zoysia japonica Steud.) and miscanthus (Miscanthus sinensis Anderss.) (2009)
  • Author(s): X. Wang, Y. Hoshino, T. Yamada
  • Organizer: 第65回日本草地学会
  • Place of Presentation: 日本大学(藤沢市)
• [Presentation] Functional genomics for winter hardiness in forage grasses (2008)
  • Author(s): H.Hisano、K.Tamura、M.Yoshida、A.Kanazawa、T.Yamada
  • Organizer: Plant & Animal Genome XVI
  • Place of Presentation: アメリカ合衆国、サンディエゴ市(招待講演)
  • Year and Date: 2008-01-12
• [Presentation] Functional genomics for winter hardiness in forage grasses (2008)
  • Author(s): H. Hisano, K. Tamura, M. Yoshida, A. Kanazawa, T. Yamada
  • Organizer: Plant & Animal Genome XVI
  • Place of Presentation: アメリカ合衆国、サンエゴ市
  • Year and Date: 2008-01-12
• [Presentation] Functional genomics for winter hardiness in forage grasses (2008)
  • Author(s): H. Hisano, K. Tamura, M. Yoshida, A. Kanazawa, T. Yamada
  • Organizer: Plant & Animal Genome XVI
  • Place of Presentation: アメリカ合衆国サンジエゴ市・タウンアンドカントリーホテル
  • Year and Date: 2008-01-12
• [Presentation] The expression regulation of multiple fructosyltransferase genes controls the fructan accumulation in correspondence with low temperature in perennial ryegrass (Lolium perenne L.) (2008)
  • Author(s): H.Hisano、A.Kanazawa、M.Yoshida、M.Humphreys、M.Iizuka、K.Kitamura、T.Yamada
  • Organizer: The Sixth International Fructan Symposium
  • Place of Presentation: 札幌市(ホテルモントレ エーデルホフ札幌)
• [Presentation] Low-temperature tolerance related CBF and fructosyltransferase genes in forage grasses (2008)
  • Author(s): H.Hisano、T.Yamada、M.Yoshida、A.Kanazawa、T.Yamada
  • Organizer: XXI International Grassland Congress
  • Place of Presentation: 中国、フフホト市(招待講演)
• [Presentation] Establishing abundant and efficient explants system for Agrobacterium-mediated transformation in zoysiagrass (2008)
  • Author(s): X.Wang、Y.Hoshino、T.Yamada
  • Organizer: XXI International Grassland Congress
  • Place of Presentation: 中国、フフホト市
• [Presentation] Establishing abundant and efficient explants system for Agrobacterium-mediated transformation in zoysiagrass (2008)
  • Author(s): X. Wang, Y. Hoshino, T. Yamada
  • Organizer: XXI Intarnational Grassland Congress
  • Place of Presentation: 中国, フフホト市
• [Presentation] Establishing abundant and efficient explant system for Agrobacterium-mediated transformation in zoysiagrass (2008)
  • Author(s): X. Wang, Y. Hoshino, T. Yamada
  • Organizer: XXI Intarnational Grassland Congress
  • Place of Presentation: フフホト国際会議場(中国フフホト市)
• [Presentation] チモシーとコムギ由来フルクタン合成酵素遺伝子を導入したイネ科モデル植物Brachypodium distachyon形質転換体の比較解析
  • Author(s): 田村健一、眞田康治、田瀬和浩、吉田みどり、山田敏彦
  • Organizer: 2011年度日本草地学会
• [Presentation] モシーとコムギ由来フルクタン合成酵素遺伝子を導入したイネ科モデル植物Brachypodium distachyon形質転換体の比較解析
  • Author(s): 田村健一、眞田康治、田瀬和浩、吉田みどり、山田敏彦
  • Organizer: 2011年度日本草地学会
  • Place of Presentation: 震災のため中止、講演要旨の配布をもって発表とみなされる
• [Book] Molecular Techniques in Crop Improvement, 2nd Edition(S.M.Jain and D.S.Brar (eds.))(Transgensis and genomics in forage crops) (2010)
  • Author(s): T.Yamada、K.Tamura、X.Wang、Y.Aoyagi
  • Publisher: Springer、the Netherlands
• [Book] Molecular Techniques in Crop Improvement, 2nd Edition(S.M. Jain & D. S. Brar (eds.), Transgensis and genomics in forage crops) (2010)
  • Author(s): T. Yamada, K. Tamura, X. Wang, Y. Aoyagi
  • Publisher: Springer
• [Book] Transgenesis and Genomics in Forage Crops In : Molecular Techniques in Crop Improvement(S.M. Jain and D.S. Brar, Eds.) (2010)
  • Author(s): T.Yamada, K.Tamura, X.Wang, Y.Aoyagi
  • Total Pages: 26
  • Publisher: Springer Science+Business Media
• [Book] Molecular Breeding of Forage and Turf (2009)
  • Author(s): T.Yamada、G.S.Spangenberg
  • Total Pages: 352
  • Publisher: Springer、New York
• [Book] Molecular Breeding of Forage and Turf (2009)
  • Author(s): T. Yamada, G. S. Spangenberg
  • Total Pages: 352
  • Publisher: Springer
• [Book] Compendium of Transgenic Crop Plants : Transgenic Cereals and Forage Grasses(C.Kole and T.C.Hall (eds.))(Cool Season Forage Grasses) (2008)
  • Author(s): Z.-Y.Wang、T.Yamada
  • Publisher: Blackwell Publishing、Oxford、UK
• [Book] Compendium of Transgenic Crop Plants : Transgenic Cereals and Forage Grasses(Kole, C. and Hall, T. C. (eds.), Cool Season Forage Grasses) (2008)
  • Author(s): Z. -Y. Wang, T. Yamada
  • Publisher: Blackwell Publishing
• [Book] Molecular Breeding of Forage and Turf (2008)
  • Author(s): T. Yamada, G. Spangenberg
  • Total Pages: 352
  • Publisher: Springer Scierice + Business Media, LLC, New York
• [Book] Cool-Season Forage Grasses, In : A Compendium of Transgenic Crop Plants : Cereals and Forage Grasses. Edited by C. Kole and T. C. Hall (2008)
  • Author(s): Z-Y. Wang, T. Yamada
  • Total Pages: 11
  • Publisher: Blackwell Publishing, Oxford, UK
• [Book] Cool-Season Forage Grasses, In: A Compendium of Transgenic Crop Plants: Cereals and Forage Grasses. Edited by C. Kole and T.C. Hall (2008)
  • Author(s): Z-Y. Wang, T. Yamada,
  • Publisher: John Wiley & Sons Ltd(印刷中)