Construction of yogurt with strong exclusion ability of harmful bacteria by probiotic blood type lactic acid bacteria

• Project/Area Number: 19380151
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Tohoku University
• Principal Investigator: SAITO Tadao Tohoku University, 大学院・農学研究科, 教授 (00118358)
• Co-Investigator(Kenkyū-buntansha): KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885) ; KAWAI Yasushi 東北大学, 大学院・農学研究科, 助教 (00261496) ; NISHIMURA Junko 東北大学, 大学院・農学研究科, 技術専門職員 (10241556)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 乳酸菌 / プロバイオティクス / 血液型乳酸菌 / 血液型有害菌 / 競合素材排除 / 機能性ヨーグルト / 競合阻害排除 / ヨーグルト

Research Abstract

A new evaluation system to select probiotic lactic acid bacteria (LAB) with adherence to human colonic mucin (HCM) by recognizing ABO-blood type antigens. LAB strains showing strong adhesion to human blood type-A antigen expressed on the intestinal mucosa were selected from 237 probiotic strains using the biosensor BIACORE. A similar method was used to select harmful bacteria that also recognizing the same antigen in the human intestine. Staphyrococcus. ssp. and E. coli were detected harmful bacteria. Using a competitive adhesion experiment with a micro plate coated with the type-A antigen and the harmful bacteria, the bacteria was significantly decreased when they were added in combination with the type-A LAB. Our data suggested the blood type LAB strains may competitively inhibit pathogenic infections where this strategy may be useful in the development of improved future probiotic foods to protect human health.

Research Products

• [Journal Article] Identification of a new adhesin-like protein form Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens (2010)
  • Author(s): Watanabe, M., H. Kinoshita, N. Nitta, R. Yukishita, Y. Kawai, K Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, K. Miura, A. Horii, H. Kitazawa, T. Saito.
  • Journal Title: J.Appl.Microbiol 108(in press)
  • Peer Reviewed
• [Journal Article] Identification of a new adhesion-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens (2010)
  • Author(s): M.Watanabe, 齋藤忠夫, et al.
  • Journal Title: Journal of Applied Microbiology 108(印刷中)
  • Peer Reviewed
• [Journal Article] Utilization of the porcine system to study lymphotoxin-β regulation in intestinal lymphoid tissue (2009)
  • Author(s): Shimazu, T., M. Tohno, S. Katoh, T., Shimosato, H. Aso, Y. Kawai, T. Saito, H. Kitazawa
  • Journal Title: Biochem.Genet. 47 Pages: 126-136
  • Peer Reviewed
• [Journal Article] オランダでの第9回乳酸菌シンポジウム(LAB9)に参加して (2009)
  • Author(s): 木下英樹, 齋藤忠夫, ら
  • Journal Title: ミルクサイエンス 58 Pages: 21-25
  • Peer Reviewed
• [Journal Article] Microbiota analysis of Caspian Sea yogurt, a ropy fermented milk circulated in Japan (2009)
  • Author(s): K.Uchida, 齋藤忠夫, et al.
  • Journal Title: Animal Science Journal 80 Pages: 187-192
  • NAID: 10027886037
  • Peer Reviewed
• [Journal Article] Cell surface Lactobacillus plantarum LA318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin (2008)
  • Author(s): Kinoshita, H., H. Uchida, Y. Kawai, T. Kawasaki, N. Wakahara, H. Matsuo, M. Watanabe, H. Kitazawa, S. Ohnuma, K. Miura, A. Horii, T. Saito.
  • Journal Title: J.Appl.Microbiol. 104 Pages: 1667-1674
  • Peer Reviewed
• [Journal Article] プロバイオティック乳酸菌の血液型抗原を介したヒト腸管付着性機構 (総説) (2008)
  • Author(s): 木下英樹, 渡辺真通, 齋藤忠夫
  • Journal Title: 日本乳酸菌学会誌 19 Pages: 78-88
  • Peer Reviewed
• [Journal Article] Identification of five phosphor-beta-glycosidases form Lactobacillus gasseri ATCC33323^T cultured in lactose medium (2008)
  • Author(s): Nagaoka, S., Honda, H., Ohshima, S., Kawai, Y., Kitazawa, H., Tateno, Y., Yamazaki, Y., T. Saito.
  • Journal Title: Biosci.Biothchnol.Biochem. 72 Pages: 1954-1957
  • Peer Reviewed
• [Journal Article] Bacteriocin production of probiotic Lactobacillus gasseri LA39 isolated from human feces in milk-based media (2008)
  • Author(s): Arakawa, K., Kawai, Y., Fujitani, K., Nishimra, J., Kitazawa, H., Komine, K., Kai, K., T. Saito.
  • Journal Title: Anim.Sci.J. 79 Pages: 634-640
  • NAID: 10025370789
  • Peer Reviewed
• [Journal Article] Microbial community analysis of food-spoilage bacteria in commercial custard creams using culture-dependent and independent methods (2008)
  • Author(s): Arakawa, K., Kawai, Y., Iioka, H., Tanioka, M., Nishimura, J., Kitazawa, H., Tsurumi, K., T. Saito.
  • Journal Title: J.Dairy Sci. 91 Pages: 2938-2946
  • Peer Reviewed
• [Journal Article] Cell surface Lactobacillus plantarum LA318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2008)
  • Author(s): H. Kinoshita, T. Saito, et.al.
  • Journal Title: Journal of Applied Microbiology 104 Pages: 1667-1674
  • Peer Reviewed
• [Journal Article] Cell surface Lactobacillus plantarum LA318 glycelaldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin (2008)
  • Author(s): H. Kinoshita, T. Saito, et. al.
  • Journal Title: Journal of Applied Microbiology 103(印刷中)
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (BIACORE assay) (2007)
  • Author(s): H. Kinoshita, Uchida, H, Kawai, Y., Kitazawa, H., Miura, K., Shiiba, K., Horii, A., T. Saito
  • Journal Title: J Appl.Microbiol. 102 Pages: 116-123
  • Peer Reviewed
• [Journal Article] β-Galactosaidase, phosphor-galactosidase and phosphor-glucosaidase activities in lactobacilli strains isolated from human faeces (2007)
  • Author(s): Honda, H., Kataoka, F., Nagaoka, S., Kawai, Y., H. Kitazawa, H. Itoh, K. Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, T. Saito.
  • Journal Title: Lett.Appl.Microbiol. 45 Pages: 461-466
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (BIACORE assay) (2007)
  • Author(s): H. Kinoshita, T. Saito, et. al.
  • Journal Title: Journal of Applied Microbiology 102 Pages: 116-123
  • Peer Reviewed
• [Presentation] ヒトABO式血液型抗原糖鎖結合性の新規ビフィズス菌の発見とその付着特性の解明 (2010)
  • Author(s): 齋藤忠夫, ら12名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] Lactobacillus gasseri OLL2716 (LG21) によるピロリ菌のヒト胃ムチン付着阻害機構の解明 (2010)
  • Author(s): 新田将光, 齋藤忠夫, ら14名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] 腸管細胞共培養系によるイムノバイオテック評価系構築 (2010)
  • Author(s): 藤江瞳, 齋藤忠夫, ら12名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] PIE細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2010)
  • Author(s): 細矢翔一, 齋藤忠夫, ら9名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] ウシ対応型の抗炎症性イムノバイオテック評価 (2010)
  • Author(s): 高梨直也, 齋藤忠夫, ら9名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] GAPDH活性を指標としてヒト腸管高付着性乳酸菌の新規スクリーニング法の確立(1) (2010)
  • Author(s): 木下英樹, 齋藤忠夫, ら12名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] GAPDH活性を指標としたヒト腸管高付着性乳酸菌の新規スクリーニング法の確立(2) (2010)
  • Author(s): 井本瞬, 齋藤忠夫, ら12名
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] 改良チーズホエイ培地を用いたバクテリオシン (ガセリシンA) の調製とカスタードクリームの腐敗抑制効果 (2010)
  • Author(s): 中村圭志, 齋藤忠夫, ら9名
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] ヒト腸管由来L.gasseriの二成分性バクテリオシン (ガセリシンT) の特性解析 (2010)
  • Author(s): 中條貴弘, 齋藤忠夫, ら8名
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都
  • Year and Date: 2010-03-28
• [Presentation] プロバイオティクスとその菌体内外成分に期待する新機能性ヨーグルトの開発研究 (2010)
  • Author(s): 齋藤忠夫
  • Organizer: 日本畜産学会主催シンポジウム
  • Place of Presentation: 東京都(招待講演)
  • Year and Date: 2010-03-27
• [Presentation] プロバイオティクスとその菌体内外成分に期待する新機能性ヨーグルトの開発研究 (2010)
  • Author(s): 齋藤忠夫
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学リバティタワー
  • Year and Date: 2010-03-27
• [Presentation] Search for probiotics with high biding activity to human colon mucin and immunomodulation activity and their applications for human health (2009)
  • Author(s): Saito, T.
  • Organizer: the 10^<th> AEARU International Molecular Biology & Biotechnology Workshop
  • Place of Presentation: Taiwan(招待講演)
  • Year and Date: 2009-11-12
• [Presentation] プロバイオティック乳酸菌を用いたCandida albicans付着阻害 (2009)
  • Author(s): 木下英樹、齋藤忠夫, ら9名
  • Organizer: 日本畜産学会大会第110回大会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-03-27
• [Presentation] ヒト腸管単離プロバイオティック乳酸菌の腸管付着性機構の解明とその応用 (2008)
  • Author(s): 齋藤忠夫, ら
  • Organizer: 日本畜産学会第109回大会
  • Place of Presentation: 常磐大学
  • Year and Date: 2008-03-27
• [Presentation] Bacterial cell surface glyceraldehydes-3-phospphate dehydrogenase (GAPDH) of Lactobaccilus plantarum recognizes human blood group antigens on mucin (2008)
  • Author(s): Kinoshita, H., T. Saito, et al.(9)
  • Organizer: The 9^<th> Symposium on Lactic Acid Bacteria, Egmond aan Zee
  • Place of Presentation: The Netherlands
• [Presentation] New strategy to exclude harmful bacreia from the human intestine using probiotic lactic acid bacteria (LAB) having specific adhesion to ABO-type human blood antigens of the intestinal mucosa (2008)
  • Author(s): T. Saito, Wakahara, M, et al.(11)
  • Organizer: The 9^<th> Symposium on Lactic Acid Bacteria, Egmond aan Zee
  • Place of Presentation: The Netherlands
• [Presentation] ヒトABO式血液型を識別するプロバイオティック乳酸菌の発見とその将来的な利用戦略 (2007)
  • Author(s): 齋藤忠夫
  • Organizer: 2007年度日本乳酸菌学会秋季セミナー
  • Place of Presentation: 東京都(招待講演)
  • Year and Date: 2007-11-16
• [Presentation] Discavary of new probiotic lactic acid bacteria (LAB) with specific adhesion to human blood ABO type antigen of intestinal mucosa and their application (2007)
  • Author(s): Saito, T.
  • Organizer: The 4^<th> Asian Conference on Lactic Acid Bacteria. The 3th International Symposium on Lactic Acid Bacteria and Health
  • Place of Presentation: Shanghai, Chaina(招待講演)
• [Presentation] Immunoregulatory Factors from Probiotics (2007)
  • Author(s): Kitazawa, H., M. Tohno, T. Saito.
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo(招待講演)
• [Book] 「医科プロバイオティクス学」(古賀泰裕 編集)(プロバイオティクスの効能と作用機序) (2009)
  • Author(s): 齋藤忠夫
• [Book] Potential for improving Health : Immunomodulation by Dairy Ingredients. In Bioactive Comoponents in Milk and Dairy Products (ed. by Y.W.Park) (2009)
  • Author(s): Saito, T.
  • Publisher: Wiley-Blackwell
• [Book] Antihypertensive peptides derived from bovine casein and whey proteins. In Bioactive Components of Milk. (ed. by Z.Mosze) (2008)
  • Author(s): Saito, T.
• [Remarks]
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