Molecular dissection of the vector-parasite relationship using Aedes-Filaria transmission system

• Project/Area Number: 19380165
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO Shinya Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 講師 (50376422)
• Co-Investigator(Kenkyū-buntansha): KANUKA Hirotaka 帯広畜産大学, 原虫病研究センター, 教授 (50342770)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: イヌ / フィラリア / ヤブカ / 寄生虫 / 宿主適応

Research Abstract

The host -parasite relationship in vector mosquito, Aedes, and Filaria parasite was analyzed. Our data indicated that the mosquito innate immune systems were not significantly affect filarial infection to mosquito vector. This result suggested the presence of completely different anti-pathogen system against filarial parasite compare to small pathogens such as protozoa, bacteria or virus. The molting related genes of filarial parasite were considered as have a key role for host transition. Especially, cut1 gene transcripts were specifically detected in host transition stage. This result indicated that cut1 could be a useful drug target for establishing novel filarial parasite infection controlling strategy.

Research Products

• [Journal Article] Calcium signal regulates temperature-dependent transformation of sporozoites in malaria parasite development. (2011)
  • Author(s): Doi Y., Shinzawa N., Fukumoto S., Okano H., Kanuka H.
  • Journal Title: Exp.Parasitol. 128 Pages: 176-180
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors : Dirofilaria immitis as a study model. (2009)
  • Author(s): Aonuma H., Yoshimura A., Perera N., Shinzawa N., Bando H., Oshiro S., Nelson B., Fukumoto S., Kanuka H.
  • Journal Title: Parasit.Vectors 2 Pages: 15-15
  • Peer Reviewed
• [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification. (2009)
  • Author(s): Perera N., Aonuma H., Yoshimura A., Teramoto T., Iseki H., Nelson B., Igarashi I., Yagi T., Fukumoto S., Kanuka H.
  • Journal Title: J.Virol.Methods. 156 Pages: 32-36
  • Peer Reviewed
• [Journal Article] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila. (2009)
  • Author(s): Shinzawa N., Nelson B., Aonuma H., Okado K., Fukumoto S., Miura M., Kanuka H.
  • Journal Title: Cell Host Microbe. 6 Pages: 244-252
  • Peer Reviewed
• [Journal Article] Immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs (2009)
  • Author(s): Fukumoto, S., Tamaki, Y., Igarashi, I., Suzuki, H., Xuan, X.
  • Journal Title: Exp Parasitol Volume: 123 Pages: 296-301
  • Peer Reviewed
• [Journal Article] Rapid recruitment of innate immunity regulates variation of intracellular pathogen resistance in Drosophila (2009)
  • Author(s): Okado, K., Shinzawa, N., Aonuma, H., Nelson, B., Fukumoto, S., Fujisaki, K., Kawazu, S., Kanuka, H.
  • Journal Title: Biochem Biophys Res Commun Volume: 379 Pages: 6-10
  • Peer Reviewed
• [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification (2009)
  • Author(s): Perera, N., Aonuma, H., Yoshimura, A., Teramoto, T., Iseki, H., Nelson, B., Igarashi, I., Yagi, T., Fukumoto, S., Kanuka, H.
  • Journal Title: J Virol Methods Volume: 156 Pages: 32-36
  • Peer Reviewed
• [Journal Article] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila (2009)
  • Author(s): Shinzawa, N., Nelson, B., Aonuma, H., Okado, K., Fukumoto, S., Miura, M., Kanuka, H.
  • Journal Title: Cell Host Microbe Volume: 6 Pages: 244-252
  • Peer Reviewed
• [Journal Article] A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes (2009)
  • Author(s): Aonuma, H., Yoshimura, A., Kobayashi, T., Okado, K., Badolo, A., Nelson, B., Kanuka, H., Fukumoto, S.
  • Journal Title: Exp Parasitol Volume: 125 Pages: 179-183
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors : Dirofilaria immitis as a study model (2009)
  • Author(s): Aonuma, H. Yoshimura, A. Perera, N. Shinzawa, N. Bando, H. Oshiro, S. Nelson, B. Fukumoto, S. Kanuka, H.
  • Journal Title: Parasit Vectors 2 Pages: 15-21
  • Peer Reviewed
• [Journal Article] Rapid recruitment of innate immunity regulates variation of intrace llular pathogen resistance in Drosophila (2009)
  • Author(s): Okado, K. Shinzawa, N. Aonuma, H. Nelson, B. Fukumoto, S. Fujisaki, K. Kawazu, S. K anuka, H.
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 6-10
  • Peer Reviewed
• [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification (2009)
  • Author(s): Perera, N. Aonuma, H. Yoshimura, A. Teramoto, T. Iseki, H. Nelson, B. Igarashi, I. Yag T. Fukumoto, S. Kanuka, H
  • Journal Title: J Virol Methods 156 Pages: 32-36
  • Peer Reviewed
• [Journal Article] Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification. (2008)
  • Author(s): Aonuma H., Suzuki M., Iseki H., Perera N., Nelson B., Igarashi I., Yagi T., Kanuka H., Fukumoto S.
  • Journal Title: Biochem.Biophys.Res.Commun. 376 Pages: 671-676
  • Peer Reviewed
• [Journal Article] Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification (2008)
  • Author(s): Aonuma, H. Suzuki, M. Iseki, H. Perera, N. Nelson, B. Igarashi, I. Yagi, T. Kanuka, H. Fukumoto, S.
  • Journal Title: Biochem Biophys Res Commun 376 Pages: 671-676
  • Peer Reviewed
• [Journal Article] Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis (2008)
  • Author(s): Gong H, Liao M, Zhou J, Hatta T, Huang P, Zhang G, Kanuka H, Nishikawa Y, Xuan X, Fujisaki K
  • Journal Title: Vet. Parasitol. 151 Pages: 268-278
  • Peer Reviewed
• [Presentation] Filarial parasites : Developmental transition mechanism mediated by environmental stimuli during a bridge between vector and host (2009)
  • Author(s): 吉村文、岡戸清、波田一誠、丹羽隆介、福本晋也、嘉糠洋陸
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県
  • Year and Date: 2009-12-12
• [Presentation] フィラリアの環境応答性トランジション機構の解明 (2009)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第55回日本寄生虫学会・日本衛生動物学会
  • Place of Presentation: 北海道
  • Year and Date: 2009-10-03
• [Presentation] Transcription-linked developmental transition in filarial parasites during a bridge between vector and host (2009)
  • Author(s): 吉村文、岡戸清、波田一誠、丹羽隆介、福本晋也、嘉糠洋陸
  • Organizer: 9th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2009-09-09
• [Presentation] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila (2009)
  • Author(s): 新澤直明、Bryce Nelson、青沼宏佳、岡戸清、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: 9th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2009-09-09
• [Presentation] フィラリアの脱皮システムトランジション (2009)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第15回分子寄生虫学ワークショップ
  • Place of Presentation: 群馬県
  • Year and Date: 2009-08-07
• [Presentation] フィラリアの生活環における環境応答性トランジション機構 (2009)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第78回日本寄生虫学会
  • Place of Presentation: 東京都
  • Year and Date: 2009-03-28
• [Presentation] フィラリアの生活環における環境応答性トランジション機構 (2009)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第78回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] フィラリア感染幼虫における環鏡依存的な脱皮関連遺伝子の探索 (2008)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-26
• [Presentation] フィラリアはどのようにして宿主体内への移行を理解するのか? (2008)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第77回日本寄生虫学会
  • Place of Presentation: 長崎県
  • Year and Date: 2008-04-02
• [Presentation] フィラリアはどのようにして宿主体内への移行を理解するのか (2008)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 第77回日本寄生虫学会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-02
• [Presentation] Interaction between vectors and parasites : Filaria behavior at blood-feeding of mosquito (2007)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Pattern recognition of Plasmodium parasite in mosquito (2007)
  • Author(s): 福本晋也、青沼宏佳、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Interaction between vectors and parasites: Filaria behavior at blood-feeding of mosquito (2007)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Why can't the insect vector be killed by an arbovirus? (2007)
  • Author(s): 寺本時靖、宮内亜宜、吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Furrowed/CTLSE1 aborts development of Plasmodium parasite in Anopheles gambiae (2007)
  • Author(s): 青沼宏佳、Stephanie Brandt、福本晋也、寺本時靖、三浦正幸、八木健、嘉糠洋陸、David Schneider
  • Organizer: EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease Vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2007-07-14
• [Presentation] Revisiting CO2-activated mosquito behavior (2007)
  • Author(s): 前川絵美、福本晋也、徳永史生、嘉糠洋陸
  • Organizer: EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease Vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2007-07-14
• [Presentation] Fly Immunity: Drosophila Host Resistance Against Bacterial Infection (2007)
  • Author(s): 新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03
• [Presentation] Fly Immunity: Diversity of Drosophila Susceptibility to Bacterial Infection (2007)
  • Author(s): 岡戸清、新澤直明、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03
• [Presentation] What Drosophila Can Tell Us About Insect-borne Diseases: Mosquito Immune Mechanisms Against Malaria Parasite (2007)
  • Author(s): 青沼宏佳、福本晋也、寺本時靖、三浦正幸、八木健、David Schneider、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03
• [Remarks] ホームページ等