Regulation of mesenchymal stem cell differentiation by growth hormone

• Project/Area Number: 19380167
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The University of Tokyo
• Principal Investigator: YAMANOUCHI Keitaro The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (70272440)
• Co-Investigator(Kenkyū-buntansha): 田中 智 東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Satoshi 東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 成長ホルモン / 間葉系幹細胞 / 脂肪細胞 / 筋衛星細胞 / 骨芽細胞 / 骨髄 / 分化 / 骨

Research Abstract

Age-related decline in growth hormone (GH) secretion results in the loss of skeletal muscle (sarcopenia) and bone mass (osteopenia). The specific aim of the present research was to test, using human GH-transgenic rats whose GH secretion is severely suppressed, whether sarcopenia and osteopenia caused by lowered secretion/action of GH is related to the decrease in the differentiation potential of mesenchymal stem cells (MSC) in skeletal muscle and bone. The results obtained clearly indicated that GH plays a role in the maintenance of MSC function both in skeletal muscle and bone.

Research Products

• [Journal Article] Skeletal muscle growth defect in human growth hormone transgenic rat is accompanied by phenotypic changes in progenitor cells. (2010)
  • Author(s): Shibata S, Ueno C, Ito T, Yamanouchi K, Matsuwaki T, Nishihara M
  • Journal Title: AGE 32 Pages: 239-253
  • Peer Reviewed
• [Journal Article] High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for re-establishing satellite cell quiescence in vivo. (2010)
  • Author(s): Yamada M, Tatsumi R, Yamanouchi K, Hosoyama Y, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE
  • Journal Title: Am J Physiol Cell Physiol 298
  • Peer Reviewed
• [Journal Article] Skeletal Muscle Growth Defect in Human Growth Hormone Transgenic Rat is Accompanied by Phenotypic Changes in Progenitor Cells. (2010)
  • Author(s): Shibata S, et al.
  • Journal Title: AGE 32(in press)
  • Peer Reviewed
• [Journal Article] Degenerative muscle fiber accelerates adipogenesis of intramuscular cells via RhoA signaling pathway. (2009)
  • Author(s): Hosoyama T, Ishiguro N, Yamanouchi K, Nishihara M
  • Journal Title: Differentiation 77 Pages: 350-359
  • Peer Reviewed
• [Journal Article] Satellite cell differentiation in goat skeletal muscle single fiber culture. (2009)
  • Author(s): Yamanouchi K, Hosoyama T, Murakami Y, Nakano SI, Nishihara M
  • Journal Title: J Reprod Dev 55 Pages: 252-255
  • NAID: 10024942233
  • Peer Reviewed
• [Journal Article] Degenerative muscle fiber accelerates adipogenesis of intramuscularcells via RhoA signaling pathway (2009)
  • Author(s): Hosoyama T
  • Journal Title: Differentiation 77 Pages: 350-359
  • Peer Reviewed
• [Journal Article] Satellite cell differentiation in goat skeletal muscle single fiber culture (2009)
  • Author(s): Yamanouchi K
  • Journal Title: J Reprod Dev 55(In press)
  • NAID: 10024942233
  • Peer Reviewed
• [Journal Article] In vivo gene transfer of PPARγ is insufficient to induce adipogenesis in skeletal muscle. (2008)
  • Author(s): Ban A, Yamanouchi K, Matsuwaki T, Nishihara M
  • Journal Title: J Vet Med Sci 70 Pages: 761-767
  • NAID: 110006878922
  • Peer Reviewed
• [Journal Article] In vivo gene transfer of PPARγ is insufficient to induce adipogenesis in skeletal muscle (2008)
  • Author(s): Ban A
  • Journal Title: J Vet Med Sci 70 Pages: 761-767
  • Peer Reviewed
• [Journal Article] Myogenic and adipogenic properties of goat skeletal muscle stem cells. (2007)
  • Author(s): Yamanouchi K, Hosoyama T, Murakami Y, Nishihara M
  • Journal Title: J Reprod Dev 53 Pages: 51-58
  • NAID: 10027327027
  • Peer Reviewed
• [Journal Article] Both PPARγ and C/EBPα are sufficient to induce transdifferentiation of goat fetal myoblasts to adipocytes. (2007)
  • Author(s): Yamanouchi K, Ban A, Shibata S, Hosoyama T, Murakami Y, Nishihara M
  • Journal Title: J Reprod Dev 53 Pages: 563-572
  • Peer Reviewed
• [Journal Article] 18α-glycyrrhetinic acid induces phenotypic changes of skeletal muscle cells to enter adipogenesis. (2007)
  • Author(s): Yamanouchi K, Yada E, Ishiguro N, Nishihara M
  • Journal Title: Cell Physiol Biochem 20 Pages: 781-790
  • Peer Reviewed
• [Journal Article] Myogenic and adipogenic properties of goat skeletal muscle stem cells (2007)
  • Author(s): Yamanouchi K
  • Journal Title: J Reprod Dev 53 Pages: 51-58
  • NAID: 10027327027
  • Peer Reviewed
• [Journal Article] Both PPARγ and C/EBPα are sufficient to induce transdifferentiation of goat fetal myoblasts to adipocytes (2007)
  • Author(s): Yamanouchi K
  • Journal Title: J Reprod Dev 53 Pages: 563-572
  • Peer Reviewed
• [Journal Article] 18α-glycyrrhetinic acid induces phenotypic changes of skeletal muscle cells to enter adipogenesis (2007)
  • Author(s): Yamanouchi K
  • Journal Title: Cell Physiol Biochem 20 Pages: 781-790
  • Peer Reviewed
• [Presentation] 骨量低下を示す成長ホルモン(GH)分泌低下モデルラットに対するGH投与の効果 (2010)
  • Author(s): 藤井崇博, 中野真一, 松脇貴志, 山内啓太郎, 西原真杉
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-26
• [Presentation] 成長ホルモン分泌低下モデルラットにおける骨量低下メカニズムの解析 (2009)
  • Author(s): 藤井崇博, 村上要介, 松脇貴志, 山内啓太郎, 西原真杉
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-25
• [Presentation] 骨格筋前駆細胞/幹細胞の脂肪分化 (2009)
  • Author(s): 山内啓太郎
  • Organizer: 第17回日本運動生理学会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-26
• [Presentation] 成長ホルモン分泌低下症モデルラットを用いた成長ホルモンによる骨髄間質細胞の分化制御機構の解明 (2009)
  • Author(s): 藤井崇博, 山内啓太郎, 松脇貴志, 西原真杉
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] 骨格筋由来高脂肪分化細胞クローンの樹立と筋・脂肪分化能の解析 (2007)
  • Author(s): 村上要介、矢田英理香、細山徹、山内啓太郎、西原真杉
  • Organizer: 第108回日本畜産学会
  • Place of Presentation: 岡山
  • Year and Date: 2007-09-26
• [Presentation] Adipogenic differentiation of skeletal muscle progenitor/stem cells. (2007)
  • Author(s): Yamanouchi K
  • Organizer: University of Wisconsin-Madison
  • Place of Presentation: Madison WI, USA
  • Year and Date: 2007-07-21
• [Presentation] Skeletal muscle fibers affect lineage specification of satellite cells through RhoA signaling pathway. (2007)
  • Author(s): Hosoyama T, Ishiguro N, Murakami Y, Yamanouchi K, Nishihara M
  • Organizer: FASEB summer research conference "Skeletal Muscle and Stem Cells"
  • Place of Presentation: Indian Wells CA, USA
  • Year and Date: 2007-07-18
• [Presentation] Cloning and characterization of highly adipogenic cells derived from rat skeletal muscle. (2007)
  • Author(s): Murakami Y, Yada E, Hosoyama T, Yamanouchi K, Nishihara M
  • Organizer: FASEB summer research conference "Skeletal Muscle and Stem Cells"
  • Place of Presentation: Indian Wells CA, USA
  • Year and Date: 2007-07-15
• [Presentation] Skeletal muscle fibers affect lineage specification of satellite cells through RhoA signaling pathway (2007)
  • Author(s): Hosoyama T
  • Organizer: FASEB summer research conference "Skeletal Muscle and Stem Cells"
  • Place of Presentation: Indian Wells CA, USA
• [Presentation] Cloning and characterization of highly adipogenic cells derived from rat skeletal muscle (2007)
  • Author(s): Murakami Y
  • Organizer: FASEB summer research conference "Skeletal Muscle and Stem Cells"
  • Place of Presentation: Indian Wells CA, USA
• [Presentation] Adipogenic differentiation of skeletal muscle progenitor/stem cells (2007)
  • Author(s): Yamanouchi K
  • Organizer: University of Wisconsin-Madison (invited seminar)
  • Place of Presentation: Madison WI, USA
• [Presentation] 骨格筋由来高脂肪分化細胞クローンの樹立と筋・脂肪分化能の解析 (2007)
  • Author(s): 村上要介
  • Organizer: 第108回 日本畜産学会
  • Place of Presentation: 岡山