Molecular imaging of prion protein by atomic force microscop yand a novel electro-spin-resonance techniques

• Project/Area Number: 19380172
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: I NANAML Osamu Hokkaido University, 大学院・獣医学研究科, 教授 (10193559)
• Co-Investigator(Kenkyū-buntansha): INABA Mutsumi 北海道大学, 大学院・獣医学研究科, 教授 (00183179) ; HORIUTCHI Motohiro 北海道大学, 大学院・獣医学研究科, 教授 (30219216) ; KUWABARA Mikinori 北海道大学, 大学院・獣医学研究科, 名誉教授 (10002081) ; ASANUMA Katetoshi 北海道大学, 大学院・獣医学研究科, 准教授 (40332473) ; 平岡 和佳子 明治大学, 理工学部, 准教授 (00212168) ; 下山 雄平 室蘭工業大学, 工学部, 教授 (50123948)
• Co-Investigator(Renkei-kenkyūsha): HIRAOKA Wakako 明治大学, 理工学部, 教授 (00212168) ; SHINOYAMA Yuhei 室蘭工業大学, 工学部, 教授 (50123948)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2007: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
• Keywords: 人畜共通感染症 / プリオン / 電子スピン共鳴(ESR) / AFM / スピンラベル法 / 動的構造変化 / 部位特異的スピンラベル法 / パルスESR / CJD / 構造生物学

Research Abstract

本研究はブリオン病の原因であるブリオンタンパク質の凝集体変化を新しい方法を用いて明らかにし、今後のプリオン病の病態解明や治療薬の評価方法を開発することを目的とした。このプリオン凝集体への変化を起こすには細胞内低pH器官であるエンドソームで起きることから、pHの低下が必須であると考えられていることからpH7.0の生理条件からpH4.0に変化させたときの構造変化を明らかにした。電子スピン共鳴法、ならびに原子間力顕微鏡を用いて家族性プリオン病でよく見られる変異体プリオンD177NはpH4.0、変性下に置くことで変異を持っていない野生型よりも効率よく線維状の凝集体が起きることが示され。凝集の引き金となる領域がタンパク中心部のβシート付近にあることが明らかとなった。今回の結果はプリオン病の原因タンパク質の構造変化を明らかにしただけでなく、今回用いた新たな方法はプリオン関連病の治療薬の評価系に用いることが可能であると考えられる。

Research Products

• [Journal Article] Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO (2008)
  • Author(s): Ogura A, Watanabe Y, Iizuka D, Yasui H, Amitani M, Kobayashi S, Kuwabara M and Inanami O
  • Journal Title: Cancer Lett 259 Pages: 71-81
  • NAID: 120000951799
  • Peer Reviewed
• [Journal Article] Inhibition of HIF-lalpha by the anticancer drug TAS106 enhances X-ray-induced apoptosis in vitro and in vivo. (2008)
  • Author(s): Yasui H, Ogura A, Asanuma T, Matsuda A, Kashiwakura I, Ku.wabara M, Inanami O.
  • Journal Title: Br. J. Cancer 299 Pages: 1442-1452
  • Peer Reviewed
• [Journal Article] A BOLD-fMRI study of cerebral activation induced by injection of algesic chemical substances into the anesthetized rat forepaw (2008)
  • Author(s): Asanuma T, Yasui H, Sato M, Inanami O, and Kuwabara M
  • Journal Title: Jpn. J. Vet. Res. 56 Pages: 99-107
  • NAID: 120000947264
  • Peer Reviewed
• [Journal Article] Regulation of cell survival and death signals induced by oxidative stress (2008)
  • Author(s): Kuwabara M, Asanuma T, Niwa K and Inanami O
  • Journal Title: J. Clin. Biochem. Nutr 43 Pages: 51-57
  • NAID: 130004879141
  • Peer Reviewed
• [Journal Article] 2008. Dynamics and local ordering of spin-labeled prion protein : an ESR simulationstudy of a highly PH-sensitive site (2008)
  • Author(s): Chiang Y.W, Otoshima Y, Watanabe Y, Inanami O, Shimoyama Y
  • Journal Title: J. Biomol. Struct Dyn 26 Pages: 355-366
  • Peer Reviewed
• [Journal Article] Visualization of the protective ability of a free radical trapping compound against rat C6 and F98 gliomas with diffusion tensor fiber tractography (2008)
  • Author(s): Asanuma T, Doblas S, Tesiram Y. A, Saunders D, Cranford R, Yasui H, Inanami O, Smith N, Floyd R. A, Kotake Y. and Towner R.A
  • Journal Title: J. Magn. Reson. Imaging 28 Pages: 574-587
  • Peer Reviewed
• [Journal Article] Diffusion tensor imaging and fiber tractography of C6 rat glioma (2008)
  • Author(s): Asanuma T, Doblas S, Tesiram Y.A, Saunders D, Cranford R, Pearson J, Abbott A, Smith N. and Towner, R.A
  • Journal Title: J. Ma. Reson. Imaging 28 Pages: 566-573
  • Peer Reviewed
• [Journal Article] Purvalanol A induces apoptosis and downregulation of antiapoptotic proteins through abrogation of phosphorylation of JAK2/STAT3 and RNA polymerase II (2008)
  • Author(s): Iizuka D, Ogura A, Kuwabara M. and Inanami O.
  • Journal Title: Anticancer Drugs 19 Pages: 565-572
  • Peer Reviewed
• [Journal Article] Momota Y, Tomizawa N. and Yasuda J. The antiproliferative effect of bovine lactoferrin on canine mammary gland tumor cells (2008)
  • Author(s): Yamada Y, Sato R, Kobayashi S, Hankanga C, Inanami O and Kuwabara M.
  • Journal Title: J. Vet. Med. Sci. 70 Pages: 443-448
  • Peer Reviewed
• [Journal Article] Effect of bovine lactoferrin on functions of activated feline peripheral blood mononuclear cells during chronic feline (2008)
  • Author(s): Kobayashi S, Sato R, Aoki T, Omoe K, Inanami O, Hankanga C, Yamada Y, Tomizawa N, Yasuda J. and Sasaki J.
  • Journal Title: immunodeficiency virus infection J. Vet. Med. Sci. 70 Pages: 429-435
  • Peer Reviewed
• [Journal Article] X irradiation combined with TNF alpha-related apoptosis-inducing ligand (TRAIL) reduces hypoxic regions of human gastric (2008)
  • Author(s): akahashi M, Yasui H, Ogura A, Asanuma T, Kubota N, Tsujitani M, Kuwabara M. and Inanami O.
  • Journal Title: adenocarcinoma xenografts in SCID mice. J. Radiat. Res. 49 Pages: 153-161
  • Peer Reviewed
• [Journal Article] Proinsulin C-peptide abrogates type-l diabetes-induced increase of renal endothelial nitric oxide synthase in rats (2008)
  • Author(s): Kamikawa A, Ishii T, Shimada K, Makondo K, Inanami O, Sakane N, Yoshida T, Saito M. and Kimura K.
  • Journal Title: Diabetes Metab. Res. Rev. 24 Pages: 331-338
  • Peer Reviewed
• [Journal Article] Individual differences in the radiosensitivity of hematopoietic progenitor cells detected in steady-state human peripheral blood (2008)
  • Author(s): Oriya A, Takahashi K, Inanami O, Miura T, be Y, Kuwabara M, Kashiwakura I.
  • Journal Title: J. Radiat. Res. 49 Pages: 113-121
  • NAID: 110006633653
  • Peer Reviewed
• [Journal Article] Instability of familial spongiform encephalopathy-related prion mutants (2008)
  • Author(s): Watanabe Y, Hiraoka.W, Shimoyama Y,Horiuchi M, Kuwabara M. and Inanami O.
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 244-249
  • NAID: 120000951795
  • Peer Reviewed
• [Journal Article] Neuron is the primary target of Ca^<2+>paradox-type insult-induced cell injury in neuron/astrocyte co-cultures (2008)
  • Author(s): Kosugi T, Kawahara K, Tanaka M, Watanabe Y. and Inanami O.
  • Journal Title: Neurochem. Int. 52 Pages: 887-896
  • Peer Reviewed
• [Journal Article] Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac / DIABLO (2008)
  • Author(s): Ogura, A., Watanabe, Y., Iizuka, D., Yasui, H., Amitani, M., Kobayashi, S., Kuwabara, M., and Inanami, O.
  • Journal Title: Cancer Lett. 259 Pages: 71-81
  • NAID: 120000951799
  • Peer Reviewed
• [Journal Article] Inhibition of HIF-lalpha by the anticancer drug TAS106 enhances X-ray-induced apoptosis in vitro and in vivo (2008)
  • Author(s): Yasui, H., Ogura, A., Asanuma, T., Matsuda, A., Kashiwakura, I., Kuwabara, M., Inanami, O.
  • Journal Title: Br. J. Cancer 299 Pages: 1442-1452
  • Peer Reviewed
• [Journal Article] A BOLD-fMRI study of cerebral activation induced by injection of algesic chemical substances into the anesthetized rat forepaw (2008)
  • Author(s): Asanuma, T., Yasui, H., Sato, M., Inanami, O. and Kuwabara, M.
  • Journal Title: Jpn. J. Vet. Res. 56 Pages: 99-107
  • NAID: 120000947264
  • Peer Reviewed
• [Journal Article] Regulation of cell survival and death signals induced by oxidative stress (2008)
  • Author(s): Kuwabara, M., Asanuma, T., Niwa. K. and Inanami. O.
  • Journal Title: J. Clin. Biochem. Nutr. 43 Pages: 51-57
  • NAID: 130004879141
  • Peer Reviewed
• [Journal Article] Shimoyama, Y. 2008. Dynamics and local ordering of_spin-labeled prion protein : an ESR simulationstudy of a highly PH-sensitive site (2008)
  • Author(s): Chiang. Y. W. Otoshima, Y., Watanabe, Y., Inanami, O.
  • Journal Title: J. Biomol. Struct Dyn. 26 Pages: 355-366
  • Peer Reviewed
• [Journal Article] Visualization of the protective ability of a free radical trapping compound against rat C6 and F98 gliomas with diffusion tensor fiber tractography (2008)
  • Author(s): Asanuma, T., Doblas, S., Tesiram, Y. A., Saunders, D., Cranford, R., Yasui, H., Inanami. O., Smith, N., Floyd, R. A., Kotake, Y. and Towner, R. A.
  • Journal Title: J. Magn. Reson. Imaging 28 Pages: 574-587
  • Peer Reviewed
• [Journal Article] Diffusion tensor imaging and fiber tractography of C6 rat glioma (2008)
  • Author(s): Asanuma, T., Doblas, S., Tesiram, Y. A., Saunlers, D., Cranford, R., Pearson, J., Abbott, A., Smith, N. and Towner, R. A.
  • Journal Title: J. Magn. Reson. Imaging 28 Pages: 566-573
  • Peer Reviewed
• [Journal Article] Purvalanol A induces apoptosis and downregulation of antiapoptotic proteins through abrogation of phosphorylation of JAK2 / STAT3 and RNA polymerase II (2008)
  • Author(s): Iizuka, D., Ogura, A., Kuwabara, M. and Inanami, O.
  • Journal Title: Anticancer Drugs 19 Pages: 565-572
  • Peer Reviewed
• [Journal Article] The antiproliferative effect of bovine lactoferrin on canine mammary gland tumor cells (2008)
  • Author(s): Yamada, Y., Sato, R., Kobayashi, S., Hankanga, C., Inanami, O., Kuwabara, M., Momota, Y., Tomizawa, N. and Yasuda, J.
  • Journal Title: J. Vet. Med. Sci. 70 Pages: 443-448
  • NAID: 130000446949
  • Peer Reviewed
• [Journal Article] Effect of bovine lactoferrin on functions of activated feline peripheral blood mononuclear cells during chronic feline immunodeficiency virus infection (2008)
  • Author(s): Kobayashi, S., Sato, R., Aoki, T., Omoe, K., Inanami, O., Hankanga, C., Yamada, Y., Tomizawa, N., Yasuda, J. and Sasaki, J.
  • Journal Title: J. Vet. Med. Sci. 70 Pages: 429-435
  • NAID: 110006688945
  • Peer Reviewed
• [Journal Article] X irradiation combined with TNF alpha-related.apoptosis-inducing ligand (TRAIL) reduces hypoxic regions of human gastric adenocarcinoma xenografts in SCID mice (2008)
  • Author(s): Takahashi, M., Yasui, H., Ogura, A., Asanuma, T., Kubota, N., Tsujitani, M., Kuwabara. M. and Inanami, O.
  • Journal Title: J. Radiat. Res. 49 Pages: 153-161
  • Peer Reviewed
• [Journal Article] Proinsulin C-peptide abrogates type-1 diabetes-induced increase of renal endothelial nitric oxide synthase in rats (2008)
  • Author(s): Kamikawa, A., Ishii, T., Shimada, K., Makondo, K., Inanami, O., Sakane, Yoshida, T., Saito, M. and Kimura. K.
  • Journal Title: Diabetes Metab. Res. Rev. 24 Pages: 331-338
  • Peer Reviewed
• [Journal Article] Individual differences in the radiosensitivity of hematopoietic progenitor cells detected in steady-state human peripheral blood (2008)
  • Author(s): Oriya, A., Takahashi, K., Inanami, O., Miura, T., Abe, Y., Kuwabara. M., Kashiwakura, I.
  • Journal Title: J. Radiat. Res. 49 Pages: 113-121
  • NAID: 110006633653
  • Peer Reviewed
• [Journal Article] Instability of familial spongiform encephalopathy-related prionmutants (2008)
  • Author(s): Watanabe, Y., Hiraoica, W., Shimoyama, Y., Horiuchi, M., Kuwabara. M. and Inanami. O.
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 244-249
  • Peer Reviewed
• [Journal Article] Neuron is the primary target of Ca^<2+> paradox-type insult-induced cell injury in neuron / astrocyte co-cultures (2008)
  • Author(s): Kosugi, T., Kawahara, K., Tanaka, M., Watanabe, Y. and Inanami, O.
  • Journal Title: Neurochem. Int. 52 Pages: 887-896
  • Peer Reviewed
• [Journal Article] Individual Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-State Human Peripheral Blood. (2008)
  • Author(s): Oriya A, Takahashi K, Inanami O, Miura T, Abe Y, Kuwabara M, Kashiwakura I.
  • Journal Title: J Radiat Res(Tokyo). 49(2) Pages: 113-121
  • NAID: 110006633653
  • Peer Reviewed
• [Journal Article] Proinsulin C-peptide abrogates type-1 diabetes-induced increase of renal endothelial nitric oxide synthase in rats. (2008)
  • Author(s): Kamikawa A, Ishii T, Shimada K, Makondo K, Inanami O, Sakane N, Yoshida T, Saito M, Kimura K.
  • Journal Title: Diabetes Metab Res Rev. 24(4) Pages: 331-338
  • Peer Reviewed
• [Journal Article] X Irradiation Combined with TNF alpha-related Apoptosis-inducing Ligand(TRAIL)Reduces Hypoxic Regions of Human Gastric Adenocarcinom a Xenografts in SCID Mice. (2008)
  • Author(s): Takahashi M, Yasui H, Ogura A, Asanuma T, Kubota N, Tsujitani M, Kuwabara M, Inanami O.
  • Journal Title: J Radiat Res(Tokyo) 49(2) Pages: 153-161
  • Peer Reviewed
• [Journal Article] Purvalanol A induces apoptosis and downregulation of antiapoptotic proteins through abrogation of phosphorylation of JAK2/STAT3 and RNA polymerase II (2008)
  • Author(s): Iizuka D, Ogura A, Kuwabara M, Inanami O.
  • Journal Title: Anti-Cancer Drugs (印刷中)
  • Peer Reviewed
• [Journal Article] Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide pzoduction ofpolymorphonuclear neutrophils from Holsteincows (2007)
  • Author(s): Ahmed M,Kimura K,Solirnan M,Yamaji D,Okamatsu-Ogura Y, Makondo k, Inanami O,Saito M.
  • Journal Title: JVetMedSci 69(2) Pages: 125-131
  • Peer Reviewed
• [Journal Article] Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106 effectively suppresses the growth of tumor cells transplanted in mice (2007)
  • Author(s): Yasui H, Inanami O, Asanuma T, Iizuka D, Nakajima T, Kon Y, Matsuda A, Kuwabara M.
  • Journal Title: Int J Radiat Oncol Biol Phys. 1 ; 68(1) Pages: 218-228
  • NAID: 120000973704
  • Peer Reviewed
• [Journal Article] Purvalanol A enhances cell killing by inhibiting up-regulation of CDC2 kinase activity in tumor cells irradiated with high doses of X rays (2007)
  • Author(s): Iizuka D, Inanami O, Kashiwakura I, Kuwabara M.
  • Journal Title: Radiat Res. 167(5) Pages: 563-571
  • Peer Reviewed
• [Journal Article] Oral administration of Antioxidant Biofactor(AOBtrade mark)ameliorates ischemia/reperfusion- induced neuronal death in the gerbil (2007)
  • Author(s): Yasui H, Asanuma T, Watanabe Y, Waki K, Inanami O, Kuwabara M.
  • Journal Title: Biofactors 29(2-3) Pages: 113-121
  • Peer Reviewed
• [Journal Article] A new amphiphilic derivative, N-{[4-(lactobionanaido)methyl]benzylidene} -1,1- dimethy1-2-(octylsulfanyl)ethyIamine N-oxide, has a protective effect against copper-induced fulminant hepatitis in Long-Evans Cinnamon rats at an extremely low concentration compared with its original form alpha-phenyl-N-(tert-butyl) nitrone (2007)
  • Author(s): Asanuma T, Yasui H, Inanami O, Waki K, Takahashi M, Iizuka D, Uemura T, Durand G, Polidori A, Kon Y, Pucci B, Kuwabara M.
  • Journal Title: Chem Biodivers 4(9) Pages: 2253-2267
  • Peer Reviewed
• [Journal Article] Enhancement of cell death by TNF alpha-related apoptosis-inducing ligand (TRAIL) in human lung carcinoma A549 cells exposed to x rays under hypoxia (2007)
  • Author(s): Takahashi M, Inanami O, Kubota N, Tsujitani M, Yasui H, Ogura A, Kuwabara M.
  • Journal Title: J Radiat Res (Tokyo) 48(6) Pages: 461-468
  • Peer Reviewed
• [Journal Article] Costimulatory effects of complement receptor type 3 and Fc receptor for IgG (FcgammaR) on superoxide production and signal transduction in bovine neutrophils (2007)
  • Author(s): Nagahata H, Higuchi H, Inanami O, Kuwabara M.
  • Journal Title: J Vet Med Sci. 69(10) Pages: 993-997
  • Peer Reviewed
• [Journal Article] G2 期チエックポイント阻害による放射線誘導細胞致死効果のメカ二ズム (2007)
  • Author(s): 飯塚大輔、稻波修、安井博宣、小倉亜希、桑原幹典
  • Journal Title: 放射線生物研究 42(4) Pages: 444-455
  • Peer Reviewed
• [Journal Article] Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows. (2007)
  • Author(s): Ahmed M, Kimura K, Soliman M, Yamaji D, Okamatsu-Ogura Y, Makondo K, Inanami O, Saito M.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 125-131
  • NAID: 130000447535
  • Peer Reviewed
• [Journal Article] Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice. (2007)
  • Author(s): Yasui H, Inanami O, Asanuma T, Iizuka D, Nakajima T, Kon Y, Matsuda A, Kuwabara M.
  • Journal Title: Int J Radiat Oncol Biol Phys. 1;68(1) Pages: 218-228
  • NAID: 120000973704
  • Peer Reviewed
• [Journal Article] Purvalanol A enhances cell killing by inhibiting up-regulation of CDC2 kinase activity in tumor cells irradiated with high doses of X rays. (2007)
  • Author(s): Iizuka D, Inanami O, Kashiwakura I, Kuwabara M.
  • Journal Title: Radiat Res. 167(5) Pages: 563-571
  • Peer Reviewed
• [Journal Article] Oral administration of Antioxidant Biofactor(AOBtrade mark)ameliorates ischemia/reperfusion-induced neuronal death in the gerbil. (2007)
  • Author(s): Yasui H, Asanuma T, Watanabe Y, Waki K, Inanami O, Kuwabara M.
  • Journal Title: Biofactors. 29(2-3) Pages: 113-121
  • Peer Reviewed
• [Journal Article] A new amphiphilic derivative, N-{[4-(lactobionamido)methyl]benzylidene}-1, 1-dimethyl-2-(octylsulfanyl)ethylamine N-oxide, has a protective effect against copper-induced fulminant hepatitis in Long-Evans Cinnamon rats at an extremely low concentration compared with its original form alpha-phenyl-N-(tert-butyl)nitrone. (2007)
  • Author(s): Asanuma T, Yasui H, Inanami O, Waki K, Takahashi M, Iizuka D, Uemura T, Durand G, Polidori A, Kon Y, Pucci B, Kuwabara M
  • Journal Title: Chem Biodivers. 4(9) Pages: 2253-2267
  • Peer Reviewed
• [Journal Article] Enhancement of cell death by TNF alpha-related apoptosis-inducing ligand(TRAIL)in human lung carcinoma A549 cells exposed to x rays under hypoxia. (2007)
  • Author(s): Takahashi M, Inanami O, Kubota N, Tsujitani M, Yasui H, Ogura A, Kuwabara M.
  • Journal Title: J Radiat Res(Tokyo). 48(6) Pages: 461-468
  • Peer Reviewed
• [Journal Article] Costimulatory effects of complement receptor type 3 and Fc receptor for IgG(FcgammaR)on superoxide production and signal transduction in bovine neutrophils. (2007)
  • Author(s): Nagahata H, Higuchi H, Inanami O, Kuwabara M.
  • Journal Title: J Vet Med Sci. 69(10) Pages: 993-997
  • Peer Reviewed
• [Journal Article] G2期チェックポイント阻害による放射線誘導細胞致死効果のメカニズム (2007)
  • Author(s): 飯塚大輔、稲波修、安井博宣、小倉亜希 桑原幹典
  • Journal Title: 放射線生物研究 42(4) Pages: 444-455
  • NAID: 40015788851
  • Peer Reviewed
• [Presentation] 移植脳腫瘍モデルにおける新規底酸素性放射線増感剤PR-350(ドラニダゾール)の放射線増感効果 (2009)
  • Author(s): 稲波修、木野潤一、浅沼武敏、安井博宣、久保田信雄、辻谷典彦、桑原幹典
  • Organizer: 第11回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良市 猿沢荘
• [Presentation] 移植脳腫瘍モデルにおける新規低酸素性放射線増感剤PR-350(ドラニダゾール)の放射線増感効果 (2009)
  • Author(s): 稲波修、木野潤一、浅沼武敏、安井博宣、久保田信雄、辻谷典彦、桑原幹典
  • Organizer: 第11回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良市猿沢荘
• [Presentation] 金ナノ粒子存腋のX線照射による細胞増殖死の増強作用 (2008)
  • Author(s): 武内亮、安井博宣、長崎幸夫、大石基、中村隆仁、稲波修
  • Organizer: 日本放射線影響学会 第51回大会
  • Place of Presentation: 北九州国際会議場(北九州市)
• [Presentation] Characterization of Cu(II)binding site in C-terminal domain of mouse prion : Site-directed spin labeling study EPR2008 (Biomedical Redox Navigation (2008)
  • Author(s): Osamu INANAMI,Yasuko WATANABE,Wakako HIROOKA,Yuhei SHIMOYAMA,Motohiro HORIUCHI and Mikinori KUWABARA
  • Organizer: JAL Resort Sea Hawk Hotel Fukuoka
  • Place of Presentation: Kyushu Japan
• [Presentation] REDOX REGULATION OF RADIATION-INDUCED CYTOCHROME-C RELEASE FROM MITOCHONDRIA IN HUMAN LUNG CARCINOMA A549 CELLS (2008)
  • Author(s): Aki OGURA,Mikinori KUWABARA,Aki HIRAYAMA,Shigeru OOWADA and Osamu INANAM
  • Organizer: EPR2008 (Biomedical Redox Navigation)
  • Place of Presentation: JAL Resort Sea Hawk Hotel Fukuoka,Kyushu Japan
• [Presentation] FeliIle immunodeficiency virus(FIV)感染初期におけるネコの好中球機能の評価ならびにウシラクトフェリン投与の影響 (2008)
  • Author(s): 小林沙織、坂下豪、永井恭子、関隆志、稲波修、安田準、佐々木重荘、佐藤れえ子
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] C6坦癌ラットを用いた新規低酸素性放射線増感剤PR-350 (ドラニダゾール)の脳腫瘍に対する薬効評価 (2008)
  • Author(s): 木野潤一、浅沼武敏、安井博宣、久保田信雄、辻谷典彦、桑原幹典、稻波修
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] FBNによるPC12細胞に対する神経様突起誘導のメカニズムに関する検討 (2008)
  • Author(s): 伊藤望、小倉亜希、渡辺康子、浅沼武敏、稲波修
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] Feline immunodeficiency virus(FIV)感染初期におけるネコの好中球機能の評価ならびにウシラクトフェリン投与の影響 (2008)
  • Author(s): 小林沙織、坂下豪、永井恭子、関隆志、稲波修、安田準、佐々木重荘、佐藤れえ子
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] C6坦癌ラットを用いた新規低酸素性放射線増感剤PR-350(ドラニダゾール)の脳腫瘍に対する薬効評価 (2008)
  • Author(s): 木野潤一、浅沼武敏、安井博宣、久保田信雄、辻谷典彦、桑原幹典、稲波修
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] PBNによるPC12細胞に対する神経様突起誘導のメカニズムに関する検討 (2008)
  • Author(s): 伊藤望、小倉亜希、渡辺康子、浅沼武敏、稲波修
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎大学
• [Presentation] Characterization of Cu (II) binding site in C-terminal domain of mouse prion : Site-directed spin labeling study (2008)
  • Author(s): Osamu INANAMI, Yasuko WATANABE, Wakako HIROOKA, Yuhei SHIMOYAMA, Motohiro HORIUCHI and Mikinori KUWABARA
  • Organizer: EPR2008 (Biomedical Redox Navigation)
  • Place of Presentation: JAL Resort Sea Hawk Hotel Fukuoka, Kyushu Japan
• [Presentation] REDOX REGULATION OF RADIATION-INDUCED CYTOCHROME-C RELEASE FROM MITOCHONDRIA IN HUMAN LUNG CARCINOMA A549 CELLS (2008)
  • Author(s): Aki OGURA,Mikinori KUWABARA, Aki HIRAYAMA, Shigeru OOWADA abd Osamu INANAMI
  • Organizer: EPR2008 (Biomedical Redox Navigation)
  • Place of Presentation: JAL Resort Sea Hawk Hote lFukuoka, Kyushu Japan
• [Presentation] 金ナノ粒子存在下でのX線照射による細胞増殖死の増強作用 (2008)
  • Author(s): 武内亮、安井博宣、長崎幸夫、大石基、中村隆仁、稲波修
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場(北九州市)
• [Presentation] Identication of Cu(II) b inding site incarb ox y-terminal domain of mouse PrPsc Site-directed spin lab study (2007)
  • Author(s): Inanami O, Watanabe Y, Hiraoka W, Shimoyama Y, Horiuchi M, Inagaki F and Kuwabara M.
  • Organizer: Society of Electron Spin Scoence and Technology (ISESS SEST)
  • Place of Presentation: Granship (Shizuoka, Japan)
  • Year and Date: 2007-11-08
• [Presentation] Identication of Cu(II)b inding site incarb ox y-terminal domain of mouse PrP^sc Site-directed spin lab study (2007)
  • Author(s): Inanami O, Watanabe Y, Hiraoka W, Shimoyama Y, Horiuchi M, Inagaki F and Kuwabara M.
  • Organizer: Society of Electron Spin Scoence and Technology(ISESS-SEST)2007
  • Place of Presentation: Granship(Shizuoka, Japan)
  • Year and Date: 2007-11-08
• [Presentation] Analysis of pH-sensitivity and conformational stability in mouse prion protein by cysteine-scanning site-directed spin labeling (2007)
  • Author(s): Watanabe Y, Hiraoka W, Shimoyama Y, Horiuchi M, Kuwabara M, and Inanami O.
  • Organizer: Society of Electron Spin Scoence and Technology(ISESS-SEST)2007
  • Place of Presentation: Granship(Shizuoka, Japan)
  • Year and Date: 2007-11-08
• [Presentation] プリオンタンパク質C末端領域の新たなCu2+結合構造の同定 (2007)
  • Author(s): 稲波修、渡辺康子、堀内基広、平岡和佳子、下山雄平、桑原幹典
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 酪農学園大学(江別市)
  • Year and Date: 2007-09-02
• [Presentation] プリオンタンパク質のpH感受性における塩橋とヒスチジン残基の役割 (2007)
  • Author(s): 渡辺康子、平岡和佳子、下山雄平、堀内基広、桑原幹典、稲波修
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 酪農学園大学(江別市)
  • Year and Date: 2007-09-02
• [Presentation] プリオンタンパク質C末端領域の新たなCu^2+結合構造の同定 (2007)
  • Author(s): 稲波修、渡辺康子、堀内基広、平岡和佳子、下山雄平、桑原幹典、
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 酪農学園大学(江別市)
  • Year and Date: 2007-09-02
• [Presentation] The Protective Effect of Mouse Prion Protein from Metal-Catalyzed Oxidative Stress (2007)
  • Author(s): Watanabe Y, Inoue T, Horiuchi M, Kuwabara M and Inanami O.
  • Organizer: Free Radical Biology & Medicine (SFRBM's 14^<th> Annual Meeting)
  • Place of Presentation: Renaissance Hotel (Ea shington, D.C., USA)
• [Presentation] Analysis of pH-sensitivity and conformational stability in mouse prion protein by cysteine-scanning site-directed spin labeling (2007)
  • Author(s): Watanabe Y, Hiraoka W, Shimoyama Y, Ho riuchi M, Kuwabara M and Inanami O.
  • Organizer: Society of Electron Spin Scoence and Technology (ISESS -SEST)
  • Place of Presentation: Granship (Shizuoka, Japan)
• [Presentation] The Protective Effect of Mouse Prion Protein from Metal-Catalyzed Oxidative Stress (2007)
  • Author(s): Watanabe Y, Inoue T, Horiuchi M, . Kuwabara M, and Inanami O.
  • Organizer: Free Radical Biology & Medicine(SFRBM's 14^th Annual Meeting)
  • Place of Presentation: Renaissance Hotel(Eashington, D.C., USA)
• [Book] 診断と治療社 (2008)
  • Author(s): 小倉亜希、安井博宣、桑原幹典、稲波修
  • Publisher: 「放射線・紫外線と酸化ストレズ」『酸化ストレスの医学』
• [Book] 『酸化ストレスの医学』(診断と治療社) (2008)
  • Author(s): 小倉亜希、安井博宣、桑原幹典、稲波修
  • Publisher: 放射線・紫外線と酸化ストレス
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/organization/radbiol/framepage6.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/organization/radbiol/naiyouent.html