Organotin resistance mechanism of marine bacteria and its application to evaluation of ecosystem

• Project/Area Number: 19380184
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Ehime University
• Principal Investigator: SUZUKI Satoru Ehime University, 沿岸環境科学研究センター, 教授 (90196816)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Shin 愛媛大学, 沿岸環境科学研究センター, 准教授 (30370266)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 有機スズ / 海洋微生物 / 耐性遺伝子 / 環境汚染 / 微生物生態系 / 耐性 / 海洋細菌

Research Abstract

Tributyltin (TBT) is a serious contaminant in marine environment. This study aimed to reveal mechanisms of TBT resistance in marine bacteria. To apply the resistance genes for evaluation method of toxicity of TBT against microbial ecosystem. We could discover new mechanisms and defined the genes. One of the genes, sugE, is applied to the biosensor system.

Research Products

• [Journal Article] Effect of tributyltin (TBT) in the metabolic activity of TBT-resistant and sensitive estuarine bacteria. (2010)
  • Author(s): Cruz, A., Oliveira, V., Baptista, I., Almeida, A., Cunha, A., Suzuki, S.
  • Journal Title: Environmetal Toxicology (in press)
• [Journal Article] Aeromonas molluscorum Av27: a potential natural tool for TBT decomtamination. Interdisciplinary Studies on Environmental Chemistry Vol.3-Biological Responses to Chemical Contaminants. (2010)
  • Author(s): Cruz, A., Henriques, I., Correia, A., Suzuki, S., Mendo, S.
  • Journal Title: Eds. N. Hamamura, S. Suzuki, S. Mendo, C. Barroso, H. Iwata, S. Tanabe Pages: 37-46
• [Journal Article] Effect of tributyltin(TBT)in the metabolic activity of estuarine bacteria (2010)
  • Author(s): Cruz, A., Oliveira, V., Baptista, I., Almeida, A., Cunha, A., Suzuki, S., Mendo, S.
  • Journal Title: Environmental Toxicology (印刷中)
  • Peer Reviewed
• [Journal Article] Aeromonas molluscorum Av27 : a potential natural tool for TBT decomtamination (2010)
  • Author(s): Cruz, A., Henriques, I., Correia, A., Suzuki, S., Mendo, S.
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry Biological Responses to Chemical Contaminants 3(印刷中)
  • Peer Reviewed
• [Journal Article] Quantative analysis of expression of tributyltin (TBT)-regulated genes in TBT-resistant Pseudomonas aeruginosa 25W (2009)
  • Author(s): Fukushima, K., Dubey, S.K., Suzuki, S.
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry Vol.2-Environmental Research in Asia(Eds. Y. Obayashi, T. Isobe, A. Subramanian, S. Suzuki and S. Tanabe) Pages: 163-166
• [Journal Article] Quantative analysis of expression of tributyltin(TBT)-regulated genes in TBT-resistant Pseudomonas aeruginosa 25W (2009)
  • Author(s): Fukushima, K., Dubey, S.K., Suzuki, S.
  • Journal Title: Interdisciplinary Studies on Environmental Chemistry Environmental Research in Asia 2 Pages: 163-166
  • Peer Reviewed
• [Journal Article] Occurrence of exo- and endopeptidases in dissolved and particulate fractions of coastal seawater (2008)
  • Author(s): Obayashi Y., Suzuki, S.
  • Journal Title: Aquatic Microbial Ecology 50 Pages: 231-237
  • Peer Reviewed
• [Journal Article] Adsorption of extracellular proteases in seawater onto filtersduring size fractionation (2008)
  • Author(s): Obayashi, Y. Suzuki, S.
  • Journal Title: Journal of Oceanography 64 Pages: 367-372
  • Peer Reviewed
• [Journal Article] High occurrence of tetracycline (TC)-resistant bacteria and TC resistance genes related to microbial diversity in sediment of Mekong river main waterway (2008)
  • Author(s): Suzuki, S., Kobayashi, T., Suehiro, F., Tuyen, BC., Tana, TS.
  • Journal Title: Microbes and Environments 23 Pages: 149-152
  • Peer Reviewed
• [Journal Article] Occurrence and diversity of the tetracycline resistance gene tet(M)in enteric bacteria of Antarctic Adelie penguins (2008)
  • Author(s): Rahman, MH., Sakamoto, KQ., Nonaka, L., Suzuki, S.
  • Journal Title: Journal of Antimicrobial Chemotherapy 62 Pages: 627-628
  • Peer Reviewed
• [Journal Article] Occurrence of two genotypes of tetracycline (TC)resistance gene tet(M)in the TC-resistant bacteria in marine sediments of Japan (2008)
  • Author(s): Rahman, HM., Nonaka, L., Tago, R., Suzuki, S
  • Journal Title: Environmental Science and Technology 42 Pages: 5055-5061
  • Peer Reviewed
• [Journal Article] Detection of the sull, sul2, and sul3 genes in sulfonamide-resistant bacteria from wastewater and shrimp ponds of north Vietnam (2008)
  • Author(s): Hoa, PTP., Nonaka, L., Viet, PH., Suzuki, S.
  • Journal Title: Science of the Total Environment 405 Pages: 377-384
  • Peer Reviewed
• [Journal Article] Occurrence of tributyltin (TBT)-resistant bacteria is not related to TBT pollution in Mekong River coastal sediment: with hypothesis of selective pressure from suspended solid (2007)
  • Author(s): Suehiro, F., Mochizuki, H., Nakamura, S., Iwata, H., Kobayashi, T., Tanabe, S., Fujimori, Y., Nishimura, F., Tuyen, B.C., Tana, S.T., Suzuki, S.
  • Journal Title: Chemosphere 68 Pages: 1459-1464
• [Journal Article] Aeromonas veronii, a tributyltin (TBT)-degrading bacterium isolated from an estuarine environment, Ria de Aveiro in Portugal. (2007)
  • Author(s): Cruz A., Caetano, T. Suzuki, S., Mendo, S.
  • Journal Title: Marine Environmental Research 64 Pages: 639-650
• [Journal Article] Distribution and diversity of tetracycline resistance genes encodingribosomal protection proteins in Mekong River sediments in Vietnam (2007)
  • Author(s): Kobayashi, T. Suehiro, F., Tuyen, B.C. and Suzuki, S.
  • Journal Title: FEMS Microbiol.Ecol 59 Pages: 729-737
  • Peer Reviewed
• [Journal Article] Occurrence of tributyltin (TBT)-resistant bacteria is not related to TBT pollution in Mekong River and coastal sediment:with a hypothesis of selective pressure from suspended solid (2007)
  • Author(s): Suehiro, F., Mochizuki, H., Nakamura, S., Iwata, H., Kobayashi, T., Tanabe, S., Fujimori, Y., Nishimura, F., Tuyen, B.C., Tana, T.S.and Suzuki, S.
  • Journal Title: Chemosphere 68 Pages: 1459-1464
  • Peer Reviewed
• [Journal Article] Aeromonas veronii,a tributyltin(TBT)degrading bacteria isolated froman estuarine environment,Ria de Averiro in Portugal (2007)
  • Author(s): Cruz, A., Caetano, T., Suzuki, S. and Mendo, S.
  • Journal Title: Mar.Environ.Res. 64 Pages: 639-650
  • Peer Reviewed
• [Journal Article] sugE-like gene of Aeromonas molluscorum Av27 is responsible for tributyltin (TBT) resistance
  • Author(s): Cruz, A., Micaelo, N.M., Felix, V., Song, J-Y., Kitamura, S-I., Suzuki, S., Mendo, S.
• [Presentation] Aeromonas molluscorum Av27 : a potential natural tool for TBT decontamination (2009)
  • Author(s): Cruz A., Henriques I., Correia A., Suzuki S., Mendo S.
  • Organizer: Biological Responses to Chemical Contaminants : from molecular to community level
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2009-09-03
• [Presentation] sugE-like gene : a gene involved in TBT resistance in Aeromonas molluscorum Av27 (2009)
  • Author(s): Cruz, A., Suzuki, S., Mendo, S.
  • Organizer: 2009 International Symposium on Environmental Science and Technology
  • Place of Presentation: Shanghai, China
  • Year and Date: 2009-06-02
• [Presentation] Aeromonas molluscorum Av27: a potential natural tool for TBT decontamination. (2009)
  • Author(s): Cruz A., Henriques I., Correia A., Suzuki S., Mendo S.
  • Organizer: Biological Responses to Chemical Contaminants: from molecular to community level
  • Place of Presentation: Aveiro, Portugal
• [Presentation] sugE-like gene: a gene involved in TBT resistance in Aeromonas molluscorum Av27. (2009)
  • Author(s): Cruz A., Suzuki S., Mendo S.
  • Organizer: 2009 International Symposium on Environmental Science and Technology
  • Place of Presentation: Shanghai, China.
• [Presentation] 環境由来の微生物リスク -実態, 評価, 近未来- (2008)
  • Author(s): 鈴木 聡, 木暮一啓
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-26
• [Presentation] 重金属存在下におけるトリブチルスズ耐性細菌Pseudoalteromonas sp. M-1のsecA遺伝子の転写発現解析 (2008)
  • Author(s): 堺弘道, 河井祐輔, 鈴木 聡
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-26
• [Presentation] Change of proteolytic activities and bacterial assemblage in storedseawater (2008)
  • Author(s): Chui Wei Bong, 大林由美子, 鈴木 聡
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-26
• [Presentation] ベトナム北部の水圏における薬剤汚染および薬剤耐性菌の出現実態 (2008)
  • Author(s): 高巣裕之, Hong Anh Duong, Pham Hung Viet, 鈴木 聡
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-26
• [Presentation] Strategies of birnaviruses to expand ecological niche. Taipei, Taiwan, June, Handbook & Abstracts 95. (2008)
  • Author(s): Suzuki, S., Kitamura, S. I., Inaba, M., Sakamoto K. Q.
  • Organizer: The 7th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-06-25
• [Presentation] Different genes coding for tributyltin (TBT) resistance in Aeromonas spp. (2008)
  • Author(s): Cruz, A., Suzuki, S., Mendos.
  • Organizer: SETAC Europe 18th Annual Meeting
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2008-03-25
• [Presentation] Relationship between tetracycline (TC) resistance in marine sediment bacteria and concentration of barium, gallium and vanadium. (2008)
  • Author(s): Rahman, M. H., Agusa, T., Takasu, H., Suzuki, S.
  • Organizer: International Symposium on Biological Responses to Chemical Pollutants : Toward Establishing an Asian Network of Environmental Toxicology
  • Place of Presentation: Matsuyama, Japan
  • Year and Date: 2008-03-06
• [Presentation] Quantitative analysis of expression of tributyltin (TBT)-regulated genes in TBT-resistant Pseudomonas aeruginosa 25w. (2008)
  • Author(s): Fukushima, K., Dubey, S.K., Suzuki, S.
  • Organizer: The 12th International Symposium on Microbial Ecology, ISME 12
  • Place of Presentation: Cairns, Australia.
• [Presentation] Different genes coding for tributyltin (TBT) resistance in Aeromonas spp. (2008)
  • Author(s): Cruz A., Suzuki S., Mendo S.
  • Organizer: SETAC Europe 18th Annual Meeting in Warsaw
  • Place of Presentation: Warsaw, Poland.
• [Presentation] 海洋細菌Pseudoalteromonas sp.M-1のSecAが関わるトリブチルスズ耐性機構 (2007)
  • Author(s): 鈴木 聡, 北村 真一, 久保田 諭
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 函館市
  • Year and Date: 2007-09-25
• [Presentation] 海洋細菌Pseudoalteromonas sp.のSecAが関わるトリブチルスズ耐性機構 (2007)
  • Author(s): 鈴木聡・北村真一・久保田諭
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 函館市
• [Presentation] Aeromonas veronii, tributyltin (TBT) resistant bacteria: future development of a biosensor for TBT detection and bioremediation (2007)
  • Author(s): Cruz A., Nonaka L., Suzuki S., Mendo S.
  • Organizer: XIII Symposium in Pesticide Chemistry
  • Place of Presentation: Piacenza, Italy.
• [Presentation] 海洋細菌Pseudoalteromonas sp. M-1のSecAが関与する有機スズ耐性機構 (2007)
  • Author(s): 鈴木聡・北村真一
  • Organizer: 日本生化学会四国支部例会
  • Place of Presentation: 高知市
• [Book] 分子でよむ環境汚染 (2009)
  • Author(s): 鈴木聡
  • Total Pages: 252
  • Publisher: 東海大学出版会
• [Book] Chemical Pollution in Indochina (2009)
  • Author(s): Suzuki, S., Takada, H. (編著)
  • Total Pages: 200
  • Publisher: Tokai University Press
• [Remarks]
  • URL: http://web.agr.ehime-u.ac.jp/~mme/
• [Remarks]
  • URL: http://web.agr.ehime-u.ac.jp/~mme/
• [Patent(Industrial Property Rights)] ウイルス捕捉用フィルタおよびその保存法 (2009)
  • Inventor(s): 鈴木聡
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Filing Date: 2009-04-17