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Creation of plants that efficiently absorb and remove formaldehyde in air by genetic engineering, and its application to ornamental foliage plants

Research Project

Project/Area Number 19380186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionKinki University

Principal Investigator

IZUI Katsura  Kinki University, 生物理工学部, 教授 (20025414)

Co-Investigator(Kenkyū-buntansha) AKITA Motomu  近畿大学, 生物理工学部, 准教授 (80258061)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywordsホルムアルデヒド / シックハウスガス / 形質転換観葉植物 / ファイトレメディエーション / メタノール資化性菌 / リブロースモノリン酸経路 / コドン最適化合成DNA / マイクロアレィ解析 / 遺伝子導入観葉植物 / 最適化合成DNA / マイクロアレイ解析 / メタノール資化性細菌 / 観葉植物 / 遺伝子操作 / 形質転換 / 光合成経路 / ファイトレメデイエーション
Research Abstract

The two enzymes which are involved in the formaldehyde (HCHO) fixation in a methanol utilizing bacterium were expressed both in Arabidopsis (At) and tobacco. The obtained transformants showed an augmented capacity of absorbing HCHO and an enhanced tolerance to HCHO. Genome-wide analysis of the genes whose expression were affected in response to HCHO, gave clues to the mechanisms of toxicity of and tolerance to HCHO. The plasmids for the efficient expression of these enzyme genes in plants were constructed, and trials were made to obtain transgenic ornamental plants which will be useful for phytoremediation of HCHO-polluted indoor air.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2010 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] Assimilation of formaldehyde in transgenic plants due to the introduction of bacterial ribulose monophosphate pathway genes.2010

    • Author(s)
      Chen, L.M., Yurimoto, H., Li, K.Z., Orita, I., Akita, M., Kato, N., Sakai, Y., Izui, K.
    • Journal Title

      Biosci. Biotechnol. Biochem 74(3)

      Pages: 627-635

    • NAID

      10027553062

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assimilation of formaldehyde in transgenic plants due to the introduction of bacterial ribulose monophosphate pathway genes2010

    • Author(s)
      Chen, Yurimoto, Li, Orita, Akita, Kato, Sakai, Izui
    • Journal Title

      Biosci.Biotechnol.Biochem. 74(印刷中)

    • NAID

      10027553062

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ホルムアルデヒドを吸収除去する植物の開発に成功2009

    • Author(s)
      泉井桂
    • Journal Title

      科研費News 3(印刷中)

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 有害なホルムアルデヒドを吸収除去する植物を開発2008

    • Author(s)
      泉井桂
    • Journal Title

      科研費NEWS 第4巻

      Pages: 10-10

    • Related Report
      2009 Final Research Report
  • [Journal Article] 有害な「ホルムアルデヒド」を吸収除去する植物を開発2008

    • Author(s)
      泉井桂
    • Journal Title

      科研費NEWS 4

      Pages: 10-10

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Purification capability of tobacco transformed with enzymes from a methylotrophic bacterium for formaldehyde.2007

    • Author(s)
      Sawada A., Oyabu T., Chen, L.M., Li, K.Z., Hirai, N., Yurimoto, H., Orita, I., Sakai, Y., Kato, N., Izui, K.
    • Journal Title

      Int. J. Phytoremediation 9

      Pages: 487-496

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification capability of tobacco transfbnned with enzymes from a methylotrophic bacterium for formaldehyde2007

    • Author(s)
      Sawada A, et. al.
    • Journal Title

      International Journal of Phytoremediation 9

      Pages: 487-496

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of phosphoenolpyruvate carboxylase is not essential for high photosynthetic rates in the C4 species Flaveria bidentis2007

    • Author(s)
      Furumoto T, et. al.
    • Journal Title

      Plant Physiol 144

      Pages: 1936-1945

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article]

    • Author(s)
      Kubo, S., Chen, L.M., Sakakibara, H.
    • Related Report
      2009 Final Research Report
  • [Presentation] Transcription analysis of formaldehyde response in wild-type and formaldehyde-tolerant transformant of Arabidopsis2010

    • Author(s)
      Kubo, S., Chen, L.M., Sakakibara, H., Orita, I., Yurimoto, H., Kato, N., Sakai, Y., Akita, M., Izui, K.
    • Organizer
      ICAR2010 (International Congress of Arabidopsis Research)
    • Place of Presentation
      Pacifico, Yokohama
    • Related Report
      2009 Final Research Report
  • [Presentation] Microarray analysis of the genes expressed upon exposure to formaldehyde in Arabidopsis2009

    • Author(s)
      Kubo, S., Sakakibara, H., Nakagiri, K., Izui, K.
    • Organizer
      The 9th International Plant Molecular Biology Congress
    • Place of Presentation
      Sain Louis, MO, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] ホルムアルデヒド曝露したシロイヌナズナにおける遺伝子発現のマイクロアレイによる解析2009

    • Author(s)
      久保森、中桐紘治、須藤恵美、榊原均、泉井桂
    • Organizer
      第50回日本植物生理学会
    • Place of Presentation
      名古屋, 名古屋大学
    • Related Report
      2009 Final Research Report
  • [Presentation] Microarray analysis of the genes expressed upon exposure to formaldehyde in Arabidopsis2009

    • Author(s)
      Kubo, Sakakibara, Nakagiri, Izui
    • Organizer
      9th International Plant Molecular Biology Congress
    • Place of Presentation
      セントルイス、ミズーリ州、アメリカ合衆国
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホルムアルデヒドに曝露したシロイヌナズナにおける遺伝子発現のマイクロアレイによる解析2009

    • Author(s)
      久保森
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] ホルムアルデヒド曝露実験系の構築とホルムアルデヒドに対するシロイヌナズナの応答のマイクロアレイによる解析2008

    • Author(s)
      久保森
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸神戸ポートアイランド(ワールド記念ホール)
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] C3、C4光合成転換植物(Eleechalis vivipara)のC4型ホスホエノールピルビン酸カルボキシラーゼの抽出法の開発2008

    • Author(s)
      阪上 綾, 他4名
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌札幌コンベンションセンター
    • Year and Date
      2008-03-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホルムアルデヒド曝露実験系の構築とホルムアルデヒドに対するシロイヌナズナの応答のマイクロアレイによる解析2008

    • Author(s)
      久保森, 中桐紘治、須藤恵美、榊原均、泉井桂
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会
    • Place of Presentation
      神戸、神戸ポートアイランド
    • Related Report
      2009 Final Research Report
  • [Presentation] Installation of a Formaldehyde-Fixation Pathway of Methylotroph as a Bypass of Carboxylation Step of the Calvin Cycle in Higher Plants2007

    • Author(s)
      Chen LM, et. al.
    • Organizer
      第14回国際光合成会議
    • Place of Presentation
      英国グラスゴー(Scotiand Exhibition Center)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.waka.kindai.ac.jp/tea/biotech/labs/cell/cell_gaiyo.html

    • Related Report
      2009 Final Research Report
  • [Remarks] 一部を載せています。

    • URL

      http://www.waka.kindai.ac.jp/tea/biotech/labs/cell/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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