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Bacterial community of crops by molecular biological methods

Research Project

Project/Area Number 19380187
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionNational Institute for Agro-Environmental Sciences

Principal Investigator

TSUSHIMA Seiya  National Institute for Agro-Environmental Sciences, 農業環境インベントリーセンター, センター長 (50354080)

Co-Investigator(Kenkyū-buntansha) TSUCHIYA Kenichi  九州大学, 大学院・農学研究院, 教授 (40150510)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords生物環境 / 葉圏細菌 / 植物 / 表生微生物 / 細菌 / 微生物
Research Abstract

There are many unculturable bacteria in environment, for example, ca.99% of bacteria in soil have been reported to be unculturable. However, little is known about bacteria on plant surface. In this study, the rapid and simple counting method of total bacteria on plant surface was developed and the populations of culturable (A) and unculturable (B) bacteria on the leaf surface of crops (rice and wheat) were assessed by the developed counting method and agar medium. The proportions (A/B) of their bacteria of rice and wheat before heading time were about 1/10 and 1/1000, respectively, indicating the significant difference in the proportion(A/B) among the two crops. The results suggest the dynamic change in the proportion of culturable bacteria on leaf surface among crops.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (39 results)

All 2010 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (12 results) Book (8 results) Remarks (9 results)

  • [Journal Article] 微生物インベントリー2009

    • Author(s)
      對馬誠也、小板橋基夫
    • Journal Title

      植物防疫 63巻

      Pages: 42-46

    • NAID

      40016485504

    • Related Report
      2009 Final Research Report
  • [Journal Article] 微生物で食の安全・安心をまもる!微生物インベントリーの構築と微生物農薬開発2009

    • Author(s)
      對馬誠也
    • Journal Title

      アグロイノベーション2009講演要旨 1

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 植物棲息微生物の新機能-生物防除、アルカロイド分解、かび毒分解能など-2009

    • Author(s)
      對馬誠也
    • Journal Title

      第15回弘前大学遺伝子実験施設シンポジウム講演要旨集 1

      Pages: 5-5

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 土壌微生物相の解明による土壌生物性の解析技術の開発2009

    • Author(s)
      對馬誠也
    • Journal Title

      有機農業研究者会議2009資料集 1

      Pages: 37-41

    • NAID

      40017015966

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Enhanced biofilm formation and 3-chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO12009

    • Author(s)
      S. Yoshida
    • Journal Title

      Journal of Applied Microbiology 106

      Pages: 790-800

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      對馬誠也
    • Journal Title

      ソフトサイエンス社

      Pages: 74-79

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 微生物インベントリー2009

    • Author(s)
      對馬誠也
    • Journal Title

      植物防疫 63

      Pages: 42-46

    • NAID

      40016485504

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Phylogenetic diversities of dominant culturable Bacillus,Pseudomonas,and Pantoea species on tomato leaves and their possibility as biological control agents.Journal of Phytopathology 155, 446-453 2007 (8).2007

    • Author(s)
      J. ENYA
    • Journal Title

      Journal of Phytopathology 155

      Pages: 446-453

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on biocontrol of air-borne disease by a filamentous fungus which pro duces antifungal volatiles.2007

    • Author(s)
      Motoo, Koitabashi
    • Journal Title

      JARQ 41

      Pages: 261-265

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Method for simply and rapidly enumerating total epiphytic bacteria in the washing solution of rice plants

    • Author(s)
      Rieko Niwa, Shigenobu Yoshida, Naruto Furuya, Kenichi Tsuchiya, Seiya Tsushima
    • Journal Title

      Canadian Journal of Microbiology (投稿中)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] ムギの葉面細菌数の動態と気象要因の関係2010

    • Author(s)
      丹羽理恵子、吉田重信、古屋成人、土屋健一、對馬誠也
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      2010年度大会講演要旨集
    • Related Report
      2009 Final Research Report
  • [Presentation] イネ表面に存在する全細菌数計測法2009

    • Author(s)
      丹羽理恵子、吉田重信、古屋成人、土屋健一、對馬誠也
    • Organizer
      日本農芸化学会大会講演要旨集
    • Related Report
      2009 Final Research Report
  • [Presentation] イネおよび生育期間における葉面細菌数の推移2009

    • Author(s)
      丹羽理恵子、吉田重信、古屋成人、土屋健一、對馬誠也
    • Organizer
      第25回日本微生物生態学会講演要旨集
    • Related Report
      2009 Final Research Report
  • [Presentation] コムギ葉鞘における棲息細菌密度とアシル化ホモセリンラクトン類生産・分解能の解析2009

    • Author(s)
      植屋由希、吉田満朗、古屋成人、吉田重信、對馬誠也、土屋健一
    • Organizer
      日植病報
    • Related Report
      2009 Final Research Report
  • [Presentation] イネおよび生育期間における葉面細菌数の推移2009

    • Author(s)
      丹羽理恵子・吉田重信・古屋成人・土屋健一・對馬誠也
    • Organizer
      第25回日本微生物生態学会
    • Place of Presentation
      広島大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] コムギ葉鞘における棲息細菌密度とアシル化ホモセリンラクトン類生産・分解能の解析2009

    • Author(s)
      植屋由希・吉田満朗・古屋成人・吉田重信・封馬誠也
    • Organizer
      日本植物病理学会
    • Place of Presentation
      山形大学(2009年3月)
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ表面に存在する全細菌数計測法2009

    • Author(s)
      丹羽理恵子
    • Organizer
      日本農芸化学学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネ葉鞘に生息する細菌の群集構造と性状解析2009

    • Author(s)
      吉田満明
    • Organizer
      日本植物病理学会
    • Place of Presentation
      山形大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] コムギ葉鞘における生息細菌密度とアシル化ホモセリンラクトン類生産・分解能の解析2009

    • Author(s)
      植屋由希
    • Organizer
      日本植物病理学会
    • Place of Presentation
      山形大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネ葉鞘における細菌相の解析2008

    • Author(s)
      吉田満明
    • Organizer
      日本植物病理学会九州部会
    • Place of Presentation
      佐賀市
    • Year and Date
      2008-11-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Deoxynivalenol-degrading bacteria isolated from phyllosphere and rhizosphere of wheat plant.2007

    • Author(s)
      Yoko Ikunaga
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Presentation] The characterization of a deoxynivalenol degadation enzyme in the bacterium isolated from a wheat field.2007

    • Author(s)
      Yukari Sato
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2007 Annual Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      百町満朗、馬誠誠也
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Final Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      對馬誠也
    • Total Pages
      20
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Final Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除ー2009

    • Author(s)
      對馬誠也
    • Total Pages
      67
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Final Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      對馬誠也、篠原弘亮
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Final Research Report
  • [Book] 微生物と植物の相互作用-病害と生物防除-2009

    • Author(s)
      吉田重信、對馬誠也
    • Publisher
      ソフトサイエンス社
    • Related Report
      2009 Final Research Report
  • [Book] 面微生物「微生物の辞典(渡邉信、西村和子、内山裕夫、奥田徹、加来久敏、広木幹也編)2008

    • Author(s)
      對馬誠也
    • Total Pages
      180
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 総合的防除「微生物の辞典(渡邉信、西村和子、内山裕夫、奥田徹、加来久敏、広木幹也編)2008

    • Author(s)
      對馬誠也
    • Total Pages
      208
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 微生物の辞典2008

    • Author(s)
      封馬誠也
    • Total Pages
      180
    • Publisher
      朝倉書店
    • Related Report
      2008 Annual Research Report
  • [Remarks] 微生物インベントリーWebサイト「microForce (マイクロフォース)」

    • URL

      http://www.niaes.affrc.go.jp/inventory/microorg/index.html

    • Related Report
      2009 Final Research Report
  • [Remarks] 對馬誠也、未知の世界の生き物たち、常用新聞(2008.11.5号)、2008

    • Related Report
      2009 Final Research Report
  • [Remarks] 對馬誠也、微生物で食の安全・安心をまもる!微生物インベントリーの構築と微生物農薬開発、アグロ・イノベーション2009要旨集、2009、S2-2-1~S2-2-6

    • Related Report
      2009 Final Research Report
  • [Remarks] 對馬誠也、植物棲息微生物の新機能-生物防除、アルカロイド分解、かび毒分解能など-、第15回弘前大学遺伝子実験施設シンポジウム講演要旨集、2009

    • Related Report
      2009 Final Research Report
  • [Remarks] 對馬誠也、土壌微生物相の解明による土壌生物性の解析技術の開発、有機農業研究者会議2009資料集、2009、37-41

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.niaes.affrc.go.jp/inventory/microorg/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.niaes.affrc.go.jp/inventry/microorg/publication.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.niaes.affrc.go.jp/researcher/tsushima_s.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.niaes.affrc.go.jp/inventry/microorg/publication.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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