Regulatory mechanisms of fertilizability of animal sperm by the surface glycans
| Project/Area Number |
19380192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
KITAJIMA Ken Nagoya University, 生物機能開発利用研究センター, 教授 (80192558)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Tsukasa 名古屋大学, 大学院・生命農学研究科, 教授 (20144131)
SATO Chihiro 名古屋大学, 生物機能開発利用研究センター, 准教授 (10343211)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 糖鎖 / flagellasialin / シアル酸 / ポリシアル酸 / 硫酸化シアル酸 / 受精 / 精子運動 / 生体膜マイクロドメイン |
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| Research Abstract |
To understand roles of the surface glycans in regulation of fertilizability of animal sperm, we focused on the glycan-rich glycoproteins whose glycan content was more than 90% by weight. The following two new findings were obtained : (1) Glycan-rich flagellar glycoproteins were discovered in pig and sea urchin, and found to regulate sperm motility through controlling the intracellular calcium ions ; (2) Another type of glycan-rich glycoproteins strongly sticking on the sperm surface was found to be originated from seminal plasma. The detachment of the glycoprotein from the surface during capacitation was related with the higher fertilizability of sperm.
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Report
(4 results)
Research Products
(16 results)
