DEVELOPMENT OF MICROCARRIER FOR INTRODUCING LARGE DNA EFFICIENTLY AND MOLECULAR CYTOLOGOCAL ANALYSIS OF ITS INTRODUCED REAGION
| Project/Area Number |
19380194
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Osaka Kyoiku University |
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Principal Investigator |
MUKAI Yasuhiko Osaka Kyoiku University, 教育学部, 教授 (30110795)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Kiichi 大阪大学, 工学研究科, 教授 (00311770)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | 巨大DNA / 形質転換 / 穀粒品質遺伝子 / 共導入 / バイオアクティブビーズ / 人工染色体 / FISH法 / 可視化 / マイクロキャリア / イネ |
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| Research Abstract |
We developed a novel system that introduced large DNA fragments of wheat including the gene cluster related to grain hardness into rice by the use of bioactive beads-mediated transformation. This transformation method does not require the special promoter and the construction of binary vectors. The fragments of wheat genome DNA were stably transmitted to offspring and the transgenes were normally expressed in transgenic rice. The location of large DNA fragments of wheat and the transgenes in rice genome were made visible by FISH and fiber FISH methods.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes2009
Author(s)
Wada N., Kajiyama S., Akiyama Y., Kawakami S., No D., Uchiyama S., Otani M., Shimada T., Nose N., Suzuki G., Mukai Y., Fukui K
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Journal Title
Plant Cell Report Vol.28
Pages: 759-768
Related Report
Peer Reviewed
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