DEVELOPMENT OF MICROCARRIER FOR INTRODUCING LARGE DNA EFFICIENTLY AND MOLECULAR CYTOLOGOCAL ANALYSIS OF ITS INTRODUCED REAGION

• Project/Area Number: 19380194
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Osaka Kyoiku University
• Principal Investigator: MUKAI Yasuhiko Osaka Kyoiku University, 教育学部, 教授 (30110795)
• Co-Investigator(Kenkyū-buntansha): FUKUI Kiichi 大阪大学, 工学研究科, 教授 (00311770)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: 巨大DNA / 形質転換 / 穀粒品質遺伝子 / 共導入 / バイオアクティブビーズ / 人工染色体 / FISH法 / 可視化 / マイクロキャリア / イネ

Research Abstract

We developed a novel system that introduced large DNA fragments of wheat including the gene cluster related to grain hardness into rice by the use of bioactive beads-mediated transformation. This transformation method does not require the special promoter and the construction of binary vectors. The fragments of wheat genome DNA were stably transmitted to offspring and the transgenes were normally expressed in transgenic rice. The location of large DNA fragments of wheat and the transgenes in rice genome were made visible by FISH and fiber FISH methods.

Research Products

• [Journal Article] The effects of puroindoline b on the ultrastracture of endosperm cells and physicochemical properties of transgenic rice plant (2010)
  • Author(s): Wada N., Kajiyama S., Cartagena J.A., Lin L., Akiyama Y., Otani M., Suzuki G., Mukai Y., Aoki N., Fukui K
  • Journal Title: Journal of Cereal Science Vol.51 Pages: 182-188
  • Peer Reviewed
• [Journal Article] The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant. (2010)
  • Author(s): Wada, Naoki
  • Journal Title: Journal of Cereal Science 51 Pages: 182-188
  • Peer Reviewed
• [Journal Article] New frontiers of chromosome sciences for sustainable agriculture (2009)
  • Author(s): Mukai Yasuhiko
  • Journal Title: Advances in Chromosome Sciences Vol.3 Pages: 25-29
  • Peer Reviewed
• [Journal Article] Transformation of rice with high molecular weight DNAs using bio-active beads method (2009)
  • Author(s): Wada N., Kajiyama S., Akiyama Y., Otani M., Shimada T., Suzuki G., Mukai Y., Fukui K
  • Journal Title: Advances in Chromosome Sciences Vol.3 Pages: 206-207
  • Peer Reviewed
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes (2009)
  • Author(s): Wada N., Kajiyama S., Akiyama Y., Kawakami S., No D., Uchiyama S., Otani M., Shimada T., Nose N., Suzuki G., Mukai Y., Fukui K
  • Journal Title: Plant Cell Report Vol.28 Pages: 759-768
  • Peer Reviewed
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes. (2009)
  • Author(s): Wada, Naoki
  • Journal Title: Plant Cell Reports 28 Pages: 759-768
  • Peer Reviewed
• [Journal Article] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauchii genes (2009)
  • Author(s): Wada, Naoki
  • Journal Title: Plant Cell Reports 28 Pages: 759-768
  • Peer Reviewed
• [Journal Article] New frontiers of chromosome scicences for sustainable agriculture (2009)
  • Author(s): Mukai, Yasuhiko
  • Journal Title: Advances in Chromosome Sciences 3(In press)
  • Peer Reviewed
• [Journal Article] Extended DNA fiber FISH in plants: Visible messages from cell nuclei (2007)
  • Author(s): Yamamoto Maki, Mukai Yasuhiko
  • Journal Title: Nucleus Vol.50 Pages: 439-452
  • Peer Reviewed
• [Journal Article] Extended DNA fiber FISH in plants: Visible messages from cell nuclei (2007)
  • Author(s): 山本真紀
  • Journal Title: Nucleus 50 Pages: 439-452
  • Peer Reviewed
• [Presentation] Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii hardness genes (2010)
  • Author(s): 和田直樹
  • Organizer: Plant & Animal Genome XVIII
  • Place of Presentation: Town & County Hotel, San Diego
  • Year and Date: 2010-01-11
• [Presentation] コムギ硬軟質に関わるfriabilin関連遺伝子群のイネへの導入と蓄積 (2009)
  • Author(s): 鈴木剛
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-26
• [Presentation] チガヤ花粉はムギ類のどのゲノムに対して半数体を誘発するか (2009)
  • Author(s): 向井康比己
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-26
• [Presentation] Transformation of rice with 1arge DNA fragments using bio-active beads method (2009)
  • Author(s): Naoki WADA
  • Organizer: 8th Osaka University-KAIST Graduate Students Symposium in Biotechnology
  • Place of Presentation: Korea Advanced Institute of Science and Technology
  • Year and Date: 2009-02-15
• [Presentation] New frontiers of chromosome sciences for sustainable agriculture (2008)
  • Author(s): 向井康比己
  • Organizer: The 3rd Asian Chromosome Colloquium
  • Place of Presentation: 大阪大学
  • Year and Date: 2008-12-03
• [Presentation] Transformation of rice with high molecular weight DNAs using bio-active beads method (2008)
  • Author(s): 和田直樹
  • Organizer: The 3rd Asian Chromosome Colloquium
  • Place of Presentation: 大阪大学
  • Year and Date: 2008-12-03
• [Presentation] New frontiers of chromosome sciences for sustainable agri culture (2008)
  • Author(s): Yasuhiko MUKAI
  • Organizer: The 3rd Asian Chromosome Co11oquiuin
  • Place of Presentation: 大阪大学
  • Year and Date: 2008-12-03
• [Presentation] 分子細胞遺伝学の進展-FISH法とGISH法 (2008)
  • Author(s): 向井康比己
  • Organizer: 日本育種学会第114回講演会シンポジウム
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-12
• [Presentation] バイオアクティブビーズ法によるハードネス遺伝子群導入イネの作出 (2008)
  • Author(s): 和田直樹
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
  • Year and Date: 2008-10-12
• [Presentation] Development of a new plant transformation system with large DNAs using bioactive beads methods (2008)
  • Author(s): 和田直樹
  • Organizer: The 1s t international global COE symposium on Bio-Environmental Chemistry
  • Place of Presentation: Hotel Hankyu EXPO Park Osaka
  • Year and Date: 2008-01-28
• [Presentation] Development of a new plant transformation system with large DNAs using bioactive beads methods (2008)
  • Author(s): 和田直樹
  • Organizer: The 1st international global COE symposium on Bio-Environmental Chemistry
  • Place of Presentation: Hotel Hankyu EXPO Park Osaka
  • Year and Date: 2008-01-28
• [Presentation] バイオアクティブビーズ法によるコムギ有用遺伝子群を含む巨大DNAのイネへの導入 (2007)
  • Author(s): 和田直樹
  • Organizer: 大阪大学イノベーションセミナー2007
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-10-22