| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Research Abstract |
I developed the technology for incorporating non-natural amino acids into proteins in living cells, and applied it for protein researches. Thus, (i) target proteins were labeled in mammalian cells, (ii) a naturally-occurring posttranslational modification was incorporated into protein at defined sites, (iii) novel protein-protein interactions were discovered by in vivo photo-crosslinking method, and (iv) non-natural amino acids were site-specifically incorporated into proteins in insect cells for the first time.
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