Structural Basis for diverse functions of the Rad18 protein
Project/Area Number |
19390012
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | Kumamoto University |
Principal Investigator |
YAMAGATA Yuriko Kumamoto University, 大学院・医学薬学研究部, 教授 (40183678)
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Project Period (FY) |
2007 – 2008
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Project Status |
Completed (Fiscal Year 2008)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2007: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
|
Keywords | 生物物理化学 / 構造生物学 / DNA修復 / 蛋白質複合体 / 蛋白質結晶化 / X線結晶構造解析 / タンパク質-DNA複合体 |
Research Abstract |
様々なDNAの損傷に対して生物はDNAを修復する様々な仕組みをもっている。そのようなDNAの修復に重要なRad18の働く仕組みをRad18相互作用するタンパク質やDNAとの複合体の結晶化を行いRad18/Rad6複合体の結晶を得た。さらにRad18のSAPドメインが複製フォーク様DNAとの結合に重要であることをドメインの構造モデルを構築することによって説明した。
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Report
(3 results)
Research Products
(31 results)
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[Journal Article] Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RADB protein triggers its recruitment to stalled replication forks.2008
Author(s)
Tsuji, Y., Watanabe, K., Araki, K., Shinohara, M., Yamagata, Y., Tsurimoto, T., Hanaoka, F., Yamamura, K., Yamaizumi, M., and Tateishi
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Journal Title
S. Genes to Cells 13
Pages: 343-354
Related Report
Peer Reviewed
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[Journal Article] Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA binding domain of the Ets transcription factor in complex with target DNA2008
Author(s)
Suwa, Y., Nakamura, T., Toma, S., Ikemizu, S., Kai H., and Yamagata Y.
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Journal Title
Acta Cryst. F64
Pages: 171-174
Related Report
Peer Reviewed
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[Journal Article] Creation and X-ray structure analysis of the tumor necrosis factor receptor-1 selective mutant of a TNF.antagonist.2008
Author(s)
Shibata, H., et al., and Yamagata, Y., Tsunoda, S., Kamada, H., Tsutsumi Y., 他22名
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Journal Title
J. Biol. Chem. 283
Pages: 998-1007
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the IL-15-IL-15Rα complex, a cytokine-receptor unit presented in trans.2007
Author(s)
Chirifu, M., Hayashi, C., Nakamura, T., Toma, S., Shuto, T., Kai, H., Yamagata, Y., Davis S.J., and Ikemizu, S.
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Journal Title
Nature Immunol 8
Pages: 1001-1007
Related Report
Peer Reviewed
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[Journal Article] Crystallographic Investigation of the Inhibition Mode of a VIM-2 Metallo-・-lactamase from Pseudomonas aeruginosa by a Mercaptocarboxylate Inhibitor.2007
Author(s)
Yamaguchi, Y., Jin, W., Matsunaga, K., Ikemizu, S., Yamagata, Y., Wachino, J., Shibata, N., Arakawa, Y., and Kurosaki H.
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Journal Title
J. Med. Chem. 50
Pages: 6647-6653
Related Report
Peer Reviewed
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