Isolation and identification of CD81 as a novel target for treatment of rheumatoid arthritis.

• Project/Area Number: 19390026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Shujitsu University
• Principal Investigator: 中西 徹 Shujitsu University, 薬学部, 教授 (30243463)
• Co-Investigator(Kenkyū-buntansha): 森 宏樹 就実大学, 薬学部, 助教 (40388989) ; 中島 利博 医療法人さわらび会福祉村病院長寿医学研究所, 研究員 (90260752) ; 浅沼 幹人 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (00273970) ; 新井 佑志 (新井 祐志) 京都府立医科大学, 医学部, 助教 (50347449)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2007: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: CD81 / リウマチ / シノビオリン / 滑膜細胞 / DNAチップ

Research Abstract

Quantitative PCR and reporter gene system showed that the stimulation of CD81 on cell surface of SW982 cells directly induced the expression of synoviolin. When siRNA of CD81 introduced into the joints of RA-model rats, this siRNA teageting CD81 ameliorated RA in these rats.

Research Products

• [Journal Article] Small interfering RNA targeting CD81 ameliorated arthritis in rats. (2009)
  • Author(s): Nakagawa S, Arai Y, Mori H, Matsushita Y, Kubo T, Nakanishi T
  • Journal Title: Biochem Biophys Res Commun 388 Pages: 467-472
• [Journal Article] ポストゲノムと神経薬理研究 (2009)
  • Author(s): 中西徹
  • Journal Title: 日本神経精神薬理学雑誌 29 Pages: 181-188
  • NAID: 10029093015
• [Journal Article] Small interfering RNA targeting CD81 ameliorated arthritis in rats (2009)
  • Author(s): Nakagawa S., et al.
  • Journal Title: Biochem Biophys Res Commun 388 Pages: 467-472
  • Peer Reviewed
• [Journal Article] Isolation of a mRNA preferentially expressed in synoviocytes from rheumatoid arthritis that is identical with lumican, which encodes a collagen binding, extracellular matrix protein. (2008)
  • Author(s): Mori H, Nishida K, Ozaki T, Inoue H, Nakanishi T
  • Journal Title: J Hard Tissue Biol 17 Pages: 125-130
  • NAID: 110007081297
• [Journal Article] ポストゲノムの炎症・リウマチ研究と創薬 (2008)
  • Author(s): 中西徹
  • Journal Title: 薬学雑誌 128 Pages: 245-245
  • NAID: 110006572177
• [Journal Article] 炎症性関節リウマチ滑膜細胞のシグナル伝達 (2008)
  • Author(s): 森宏樹、中西徹
  • Journal Title: 薬学雑誌 128 Pages: 263-268
  • NAID: 110006572180
• [Journal Article] Isolation of a mRNA preferentially expressed in synoviocytes from rheumatoid arthritis that is identical with lumican, which encodes a collagen binding, extracellular matrix protein. (2008)
  • Author(s): Mori H, et al.
  • Journal Title: J Hard Tissue Biol 17 Pages: 125-130
  • Peer Reviewed
• [Journal Article] Connective tissue growth factor is overexpressed in muscles of human muscular dystrophy. (2008)
  • Author(s): Sun G, et al.
  • Journal Title: J Neurol Sci 267 Pages: 48-56
  • Peer Reviewed
• [Journal Article] ポストゲノムの炎症・リウマチ研究と創薬 (2008)
  • Author(s): 中西 徹
  • Journal Title: 薬学雑誌 128 Pages: 245-245
  • NAID: 110006572177
  • Peer Reviewed
• [Journal Article] 炎症性関節リウマチ滑膜細胞のシグナル伝達 (2008)
  • Author(s): 森 宏樹、中西 徹
  • Journal Title: 薬学雑誌 128 Pages: 263-268
  • NAID: 110006572180
  • Peer Reviewed
• [Journal Article] Characterization and gene expression profiling of human mesenchymal stem cells. (2007)
  • Author(s): Mori H, Itano Y, Kobayashi N, Kosaka Y, Tanaka N, Nakanishi T
  • Journal Title: J Hard Tissue Biol 16 Pages: 36-41
  • NAID: 110006250205
• [Journal Article] Characterization and gene expression profiling of human mesenchymal stem cells (2007)
  • Author(s): Mori H., et. al.
  • Journal Title: J Hard Tissue Biol 16 Pages: 36-41
  • Peer Reviewed
• [Presentation] 関節リウマチ滑膜細胞におけるテトラスパニンの機能解析 (2009)
  • Author(s): 森宏樹, 他
  • Organizer: 日本分子生物学会(ワークショップオーガナイザー)
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Application of Tetraspanin CD81 RNAi for Diagnosis and Therapy of RA (2009)
  • Author(s): Nakanishi T.
  • Organizer: CEMD-2009
  • Place of Presentation: Beijin
  • Year and Date: 2009-11-21
• [Presentation] 関節リウマチ滑膜細胞におけるテトラスパニンの機能解析 (2009)
  • Author(s): 森宏樹, 他
  • Organizer: 日本生化学会(シンポジウムオーガナイザー)
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] 関節炎におけるテトラスパニンの機能解析 (2009)
  • Author(s): 松下有美, 森宏樹, 中西徹
  • Organizer: 第129回日本薬学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2009-03-27
• [Presentation] DNAマイクロアレイによる関節リウマチ滑膜関連遺伝子の単離とWISP遺伝子を中心とするその解析(学会賞受賞講演) (2008)
  • Author(s): 森 宏樹, 中西 徹
  • Organizer: 第17回硬組織再生生物学会
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2008-08-30
• [Presentation] 関節リウマチ滑膜細胞におけるクラスター分化抗原CD81の役割 (2007)
  • Author(s): 森 宏樹、中西 徹
  • Organizer: 第17回硬組織再生生物学会
  • Place of Presentation: 松本歯科大学
  • Year and Date: 2007-09-22
• [Remarks] 新聞発表;関節リウマチの一因同定山陽新聞(2009.1.6)、産経新聞(2009.1.15)