• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the transport of proteins in alveolar type I/II epithelial cells for the development of new pulmonary DDS systems

Research Project

Project/Area Number 19390043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionHiroshima University

Principal Investigator

TAKANO Mikihisa  Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (20211336)

Co-Investigator(Kenkyū-buntansha) 永井 純也  広島大学, 大学院・医歯薬学総合研究科, 准教授 (20301301)
湯元 良子  広島大学, 大学院・医歯薬学総合研究科, 助教 (70379915)
Co-Investigator(Renkei-kenkyūsha) NAGAI Junya  広島大学, 大学院・医歯薬学総合研究科, 准教授 (20301301)
YUMOTO Ryoko  広島大学, 大学院・医歯薬学総合研究科, 助教 (70379915)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywordsドラッグデリバリー / 経肺投与 / 経肺投与DDS / 肺胞上皮細胞 / インスリン / エンドサイトーシス / カチオン性ポリアミノ酸 / ダイナミン / 経肺投与ドラッグデリバリー / タンパク質 / クラスリン / カベオラ / 初代培養細胞 / RLE-6TN細胞
Research Abstract

The uptake of albumin by primary cultured alveolar type II cells was higher than that by type I cells, and was clathrin-mediated endocytosis. Insulin uptake rate was similar between type II and type I cells, and clathrin-mediated system would not be so important in both cells. Insulin was taken up by endocytosis in RLE-6TN cells, and the uptake was markedly stimulated by cationic polyamino acids. The stimulation of insulin absorption from the lung by polyamino acids was also observed in vivo. These results provide important information for the development of new pulmonary drug delivery systems.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2010 2009 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (26 results)

  • [Journal Article] Mechanism of insulin uptake in rat alveolar type II and type I-like epithelial cells.2009

    • Author(s)
      Ikehata, M., Yumoto, R., Kato, Y., Nagai, J., Takano, M
    • Journal Title

      Biol. Pharm. Bull. 32(10)

      Pages: 1765-1769

    • NAID

      130000116940

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of insulin uptake in rat alveolar type II and type I-like epithelial cells2009

    • Author(s)
      Ikehata, M., et al.
    • Journal Title

      Biological & Pharmaceutical Bulletin 32(10)

      Pages: 1765-1769

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of albumin uptake in rat alveolar type II and type I-like epithelial cells in primary culture.2008

    • Author(s)
      Ikehata, M., Yumoto, R., Nakamura, K., Nagai, J., Takano, M.
    • Journal Title

      Pharm. Res. 25(4)

      Pages: 913-922

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low-affinity transport of FITC-albumin in alveolar type II epithelial cell line RLE-6TN.2008

    • Author(s)
      Tagawa, M., Yumoto, R., Oda, K., Nagai, J., Takano, M.
    • Journal Title

      Drug Metab. Pharmacokinet. 23(5)

      Pages: 318-327

    • NAID

      10024384520

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of albumin uptake in rat alveolar type II and type I-like epithelial cells in primary culture2008

    • Author(s)
      Ikehata, M., et al.
    • Journal Title

      Pharmaceutical Research 25(4)

      Pages: 913-922

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-affinity transport of FITC-albumin in alveolar type II epithelial cell line RLE-6TN2008

    • Author(s)
      Tagawa, M., et al.
    • Journal Title

      Drug Metabolism and Pharmacokinetics 23(5)

      Pages: 318-327

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of albumin uptake in rat alveolar type II and type I-like epithelial cells in primary culture2008

    • Author(s)
      Ikehata, M.
    • Journal Title

      Pharm, Res. (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ラット肺胞上皮細胞の形態・機能に及ぼす喫煙関連物質の影響2010

    • Author(s)
      湯元良子
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山桃太郎アリーナ
    • Related Report
      2009 Final Research Report
  • [Presentation] ラット肺胞上皮細胞の形態・機能に及ぼす喫煙関連物質の影響2010

    • Author(s)
      湯元良子, 外
    • Organizer
      第130年会日本薬学会
    • Place of Presentation
      岡山桃太郎アリーナ
    • Related Report
      2009 Annual Research Report
  • [Presentation] 肺胞上皮細胞のviabilityと遺伝子発現に及ぼす喫煙関連物質の影響2009

    • Author(s)
      加藤祐貴
    • Organizer
      第48回日本薬学会中国四国支部学術大会
    • Place of Presentation
      アスティとくしま
    • Related Report
      2009 Final Research Report
  • [Presentation] 肺胞上皮II型細胞とI型細胞における物質輸送システムの発現・機能の比較解析2009

    • Author(s)
      湯元良子
    • Organizer
      日本膜学会第31年会
    • Place of Presentation
      東京理科大学森戸記念館
    • Related Report
      2009 Final Research Report
  • [Presentation] ラット肺胞上皮II型・I型細胞におけるP-glycoproteinの発現・機能の比較解析とステロイド処置の影響2009

    • Author(s)
      加藤祐貴
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都国際会館
    • Related Report
      2009 Final Research Report
  • [Presentation] 肺胞上皮II型細胞とI型細胞における物質輸送システムの発現・機能の比較解析2009

    • Author(s)
      湯元良子, 外
    • Organizer
      日本膜学第31年会
    • Place of Presentation
      東京理科大学 森戸記念館
    • Related Report
      2009 Annual Research Report
  • [Presentation] 肺胞上皮細胞の viability と遺伝子発現に及ぼす喫煙関連物質の影響2009

    • Author(s)
      加藤祐貴, 外
    • Organizer
      第48回日本薬学会中国四国支部学術大会
    • Place of Presentation
      アスティとくしま
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラット肺胞上皮H型・I型細胞におけるP-glycoproteinの発現. 機能の比較解析とステロイド処置の影響2009

    • Author(s)
      加藤 祐貴
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット肺胞上皮細胞におけるP-glycoproteinの発現・機能とステロイドの影響2008

    • Author(s)
      加藤祐貴
    • Organizer
      第47回日本薬学会中国四国支部学術大会
    • Place of Presentation
      岡山コンベンションセンター(6)
    • Related Report
      2009 Final Research Report
  • [Presentation] Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture2008

    • Author(s)
      Mika Ikehata
    • Organizer
      第23回日本薬物動態学会年会
    • Place of Presentation
      熊本市民会館
    • Related Report
      2009 Final Research Report
  • [Presentation] MECHANISM OF INSULIN UPTAKE AND EFFECT OF POLY(AMINO ACID)S IN RAT ALVEOLAR TYPE II EPITHELIAL CELL LINE RLE-6TN2008

    • Author(s)
      Keisuke Oda
    • Organizer
      第23回日本薬物動態学会年会
    • Place of Presentation
      熊本市民会館
    • Related Report
      2009 Final Research Report
  • [Presentation] ラット肺胞II型-I型上皮細胞におけるアルブミンの取り込み機構と細胞内挙動2008

    • Author(s)
      池畑美香
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜みなとみらいホール
    • Related Report
      2009 Final Research Report
  • [Presentation] 肺胞上皮細胞RLE-6TNにおけるインスリンの輸送機構解析2008

    • Author(s)
      小田啓祐
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜みなとみらいホール
    • Related Report
      2009 Final Research Report
  • [Presentation] 肺胞上皮細胞RLE-6TNにおけるインスリンの輸送機構およびポリアミノ酸の影響解析2008

    • Author(s)
      小田 啓祐
    • Organizer
      第23年会日本薬剤学会
    • Place of Presentation
      札幌市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture2008

    • Author(s)
      池畑 美香
    • Organizer
      第23回日本薬物動態学会年会
    • Place of Presentation
      熊本市
    • Related Report
      2008 Annual Research Report
  • [Presentation] MECHANISM OF INSULIN UPTAKE AND EFFECT OF POLY(AMINOACID) S IN RAT ALVEOLAR TYPE II EPITHELIAL CELL LINERLE-6TN2008

    • Author(s)
      小田 啓祐
    • Organizer
      第23回日本薬物動態学会年会
    • Place of Presentation
      熊本市
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット肺胞上皮細胞におけるP-glycoproteinの発現・機能とステロイドの影響2008

    • Author(s)
      加藤 祐貴
    • Organizer
      第47回日本薬学会中国四国支部学術大会
    • Place of Presentation
      岡山市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺胞上皮細胞におけるインスリンの輸送とその制御2008

    • Author(s)
      湯元 良子
    • Organizer
      膜シンポジウム2008
    • Place of Presentation
      豊中市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺胞上皮細胞RLE-6TNにおけるインスリンの輸送機構解析2008

    • Author(s)
      小田 啓祐
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラット肺胞II型-I型上皮細胞におけるアルブミンの取り込み機構と細胞内挙動2008

    • Author(s)
      池畑 美香
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 肺胞上皮細胞におけるアルブミンの輸送機構2007

    • Author(s)
      湯元良子
    • Organizer
      膜シンポジウ2007
    • Place of Presentation
      山口大学工学部
    • Related Report
      2009 Final Research Report
  • [Presentation] Endocytosis of albumin and insulin in alveolar type II epithelial cell line RLE-6TN:International Society for the Study of Xenobiotics (ISSX) 8th2007

    • Author(s)
      Maki Tagawa
    • Organizer
      International Meeting
    • Place of Presentation
      仙台国際センター
    • Related Report
      2009 Final Research Report
  • [Presentation] ラット肺胞上皮細胞のII型-I型形態転換と両細胞におけるアルブミン輸送の比較解析2007

    • Author(s)
      池畑 美香
    • Organizer
      日本薬剤学会第22年会
    • Place of Presentation
      さいたま市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Endocytosis of albumin in primary cultured alveolar type II and type I-like epithelial cells2007

    • Author(s)
      Mika Ikehata
    • Organizer
      4th World Conference on Drug Absorption, Transport and Delivery
    • Place of Presentation
      金沢市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Endocytosis of albumin and insulin in alveolar type II epithelial cell line RLE-6TN2007

    • Author(s)
      Maki Tagawa
    • Organizer
      International Society for the Study of Xenobiotics (ISSX) 8th International Meeting
    • Place of Presentation
      仙台市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 肺胞上皮細胞におけるアルブミンの輸送機構2007

    • Author(s)
      湯元 良子
    • Organizer
      膜シンポジウム2007
    • Place of Presentation
      山口市
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi