Cell biological analysis of cytoplasmic lipid droplets

• Project/Area Number: 19390049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Nagoya University
• Principal Investigator: FUJIMOTO Toyoshi Nagoya University, 大学院・医学系研究科, 教授 (50115929)
• Co-Investigator(Kenkyū-buntansha): FUJITA Akikazuc 名古屋大学, 大学院・医学系研究科, 准教授 (60282232) ; OHSAKI Yuki 名古屋大学, 大学院・医学系研究科, 助教 (00378027) ; SUZUKI Michitaka 名古屋大学, 大学院・医学系研究科, 助教 (80456649)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 脂肪滴 / 小胞体 / リポ蛋白質 / 電子顕微鏡 / ApoB / Brefeldin A / CtBP / BARS / ADPリボシル化 / 脂質滴 / コレステロール

Research Abstract

肝細胞の脂肪滴について次のような成果を得た.
(1) プロテアソーム阻害, 多価不飽和脂肪酸投与などにより, 小胞体内腔と細胞質の両方に面する特殊な脂肪滴が出現することを見出し, 脂質エステルが小胞体膜中に蓄積し得ることを明確に示した.
(2) 脂質付加後のApolipoprotein Bは小胞体の内腔にあるため, 同分子が細胞質側でプロテアソーム分解を受けるためには特殊な機構が必要であることが示唆された.

Research Products

• [Journal Article] Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100 (2008)
  • Author(s): Ohsaki, Y., Cheng, J., Suzuki, M., Fujita, A., Fujimoto, T.
  • Journal Title: J. Cell Sci 121 Pages: 2415-2422
  • Peer Reviewed
• [Journal Article] FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets (2008)
  • Author(s): Nishino, N., Fujimoto, T(他25名)
  • Journal Title: J. Clin. Invest 118 Pages: 2808-2821
  • Peer Reviewed
• [Journal Article] Lipid droplet-associated proteins protect renal tubular cells from fatty acid-induced apoptosis. Am (2008)
  • Author(s): Urahama, Y., Ohsaki, Y., Fujita, Y., Maruyama, S., Yuzawa, Y., Matsuo, S., Fujimoto, T.
  • Journal Title: J. Pathol 173 Pages: 1286-1294
  • Peer Reviewed
• [Journal Article] Lipid droplets : a classic organelle with a new outfit. Histochem (2008)
  • Author(s): Fujimoto, T., Ohsaki, Y., Cheng, J., Suzuki, M., Shinohara, Y.
  • Journal Title: Cell Biol 136 Pages: 263-279
  • Peer Reviewed
• [Journal Article] 脂質滴の形成メカニズム (2008)
  • Author(s): 藤本豊士, 大崎雄樹, 鈴木倫毅
  • Journal Title: 膜 33 Pages: 17-23
• [Journal Article] Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100. (2008)
  • Author(s): Ohsaki, Y., Cheng, J., Sxizuki, M., Fujita, A., Fujimoto, T.
  • Journal Title: J. Cell Sci. 121 Pages: 2415-2422
  • Peer Reviewed
• [Journal Article] Lipid droplet-associated proteins protect renal tubular cells from fatty acid-induced apoptosis. (2008)
  • Author(s): Urahama, Y., Ohsaki, Y. Fujita, Y., Maruyama, S., Yuzawa, Y., Matsuo, S., Fujimoto, T.
  • Journal Title: Am. J. Pathol. 173 Pages: 1286-1294
  • Peer Reviewed
• [Journal Article] Lipid droplets : a classic organelle with a new outfit. (2008)
  • Author(s): Fujimoto, T., Ohsaki, Y., Cheng, J., Suzuki, M., Shinohara, Y.
  • Journal Title: Histochem. Cell Biol. 136 Pages: 263-279
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor signals play critical roles in differentiation of longitudinal smooth muscle cells inmouse embryonic gut (2008)
  • Author(s): Kurahashi, M., Niwa, Y., Cheng, J., Ohsaki, Y., Fujita, A., Goto, H., Fujimoto, T., and Torihashi, S.
  • Journal Title: Neurogastroenterol. Motil. (in press)
  • Peer Reviewed
• [Journal Article] 脂肪滴形成のメカニズム (2008)
  • Author(s): 藤本豊士, 大崎雄樹, 鈴木倫毅
  • Journal Title: 膜 33 Pages: 17-23
  • NAID: 10020130439
  • Peer Reviewed
• [Journal Article] Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling (2007)
  • Author(s): Fujita, A., J. Cheng, M. Hirakawa, K. Furukawa, S. Kusunoki, T. Fujimoto
  • Journal Title: Mol. Biol. Cell 18 Pages: 2812-2822
  • Peer Reviewed
• [Journal Article] All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium. Invest (2007)
  • Author(s): Tsuiki, E., Fujita, A., Ohsaki, Y, J. Cheng, K. Yoshikawa, H. Senoo, K. Mishima, T. Kitaoka, T. Fujimoto
  • Journal Title: 藤田秋一, 藤本豊士 48 Pages: 2858-2867
  • Peer Reviewed
• [Journal Article] Quantitative retention of membrane lipids in the freeze-fracture replica. Histochem (2007)
  • Author(s): Fujita, A. and T. Fujimoto
  • Journal Title: Cell Biol 125 Pages: 385-389
  • Peer Reviewed
• [Journal Article] 凍結レプリカ標識法による膜脂質の分布解析 (2007)
  • Author(s): 藤田秋一, 藤本豊士
  • Journal Title: 細胞工学 26 Pages: 314-319
• [Journal Article] All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium (2007)
  • Author(s): Tsuiki, E., Fujita, A., Ohsaki, Y., Cheng, J., Irie, T., Yoshikawa, K., Senoo, H., Mishima, K., Kitaoka, T., and Fujimoto, T.
  • Journal Title: Invest. Ophthal. Vis. Sci. 48 Pages: 2858-2867
  • Peer Reviewed
• [Journal Article] Biogenesis of cytoplasmic lipid droplets : from the lipid ester globule in the membrane to the visible structure
  • Author(s): Ohsaki, Y Cheng, J., Suzuki, M., Shinohara, Y., Fujita, A., Fujimoto, T.
  • Journal Title: Biochim. Biophys. Acta (in press)
  • Peer Reviewed
• [Journal Article] Segregation of GM1 and GM3 clusters in the cell membrane depends on the intact actin cytoskeleton
  • Author(s): Fujita, A., Fujimoto, T.
  • Journal Title: Biochim. Biophys. Acta (in press)
  • Peer Reviewed
• [Presentation] Lipid droplets as a new functional platform (2009)
  • Author(s): Fujimoto, T., Ohsaki, Y., Suzuki, M., Fujita, A., Cheng, J., Shinohara, Y.
  • Organizer: The 6^<th> Symposium of the Asian Biophysics Association
  • Place of Presentation: Hong Kong
• [Presentation] 細胞膜脂質のナノスケール局在解析 (2008)
  • Author(s): 藤田秋一, 程晶磊, 佐藤久美, 藤本豊士
  • Organizer: 日本薬学会第128年会, シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-26
• [Presentation] 脂質超分子構造を電顕で解析する (2008)
  • Author(s): 藤本豊士
  • Organizer: 第32回顕微鏡学会関東支部講演会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-08
• [Presentation] 脂質超分子構造を電顕で解析する (2008)
  • Author(s): 藤本豊士
  • Organizer: 第32回(平成19年度)顕微鏡学会関東支部講演会
  • Place of Presentation: 東京工業大学デジタル多目的ホール
  • Year and Date: 2008-03-08
• [Presentation] 凍結割断レプリカ法標識法による膜脂質のナノ局在解析 (2008)
  • Author(s): 藤本豊士, 藤田秋一, 程晶磊
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会年会合同大会(BMB2008), シンポジウム
  • Place of Presentation: 神戸
• [Presentation] 膜脂質ドメインの形態学的解析 (2008)
  • Author(s): 藤本豊士
  • Organizer: 特定領域研究「生体膜トランスポートゾームの分子構築と生理機能」平成20年度班会議
  • Place of Presentation: 淡路島
• [Presentation] Analysis of lipid supramolecular structures by electron microscopy (2008)
  • Author(s): Fujimoto, T
  • Organizer: The 13th International Congress on Histochemistry and Cytochemistry (ICHC2008), Symposium
  • Place of Presentation: Gdansk, Poland
• [Presentation] (2008)
  • Author(s): 藤本豊士, 藤田秋一, 程晶磊
  • Organizer: 第60回日本細胞生物学会大会, ミニシンポジウム
  • Place of Presentation: 横浜
• [Presentation] 電子顕微鏡による膜脂質のナノ局在解析 (2008)
  • Author(s): 藤本豊士, 藤田秋一, 程晶磊
  • Organizer: 第113回日本解剖学会総会・全国学術集会, シンポジウム
  • Place of Presentation: 大分
• [Presentation] Analysis of lipid supramolecular structures by electron microscopy (2008)
  • Author(s): Fujimoto, T.
  • Organizer: The 13th International Congress on Histochemistry and Cytochemistry (ICHC2008)
  • Place of Presentation: Gdansk, Poland
• [Presentation] 脂質超分子構造を電顕で見る (2007)
  • Author(s): 藤本豊士
  • Organizer: 日本学術会議シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-09-08
• [Presentation] 脂肪滴構造と機能の解析 (2007)
  • Author(s): 藤本豊士, 大崎雄樹
  • Organizer: 第51回日本顕微鏡学会シンポジウム
  • Place of Presentation: 徳島
• [Book] 蛋白質と脂質を見るための電子顕微鏡法 (2008)
  • Author(s): 藤田秋一, 藤本豊士
  • Publisher: 日本組織細胞化学会「組織細胞化学2008」
• [Book] 山本章嗣(監修) : 電子顕微鏡で読み解く生命のなぞ. ナノワールドに迫るパワフル技術入門 (2008)
  • Author(s): 藤本豊士
  • Total Pages: 160
  • Publisher: 秀潤社
• [Book] 蛋白質と脂質を見るための電子顕微鏡法 (2008)
  • Author(s): 藤田秋一, 藤本豊士
  • Publisher: 学際企画(印刷中)
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/cel-bio/index.htm
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/cel-bio/index.htm