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Post-translational ,protein modification induced with reactive species in metabolic syndrome

Research Project

Project/Area Number 19390090
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKeio University

Principal Investigator

ADACHI Takeshi  Keio University, 医学部, 講師 (50231931)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Michiko  慶應義塾大学, 医学部, 助教 (60445450)
IZUMI Yotaroy  慶應義塾大学, 医学部, 助教 (90245506)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords分子病態学 / メタボリック・シンドローム / プロテオミクス / インスリン / 血管内皮細胞 / メタボリックシンドローム
Research Abstract

Metabolic syndrome is a complex diseases, which is closely associated with insulin resistance. In this study, we characterized aorta from Zucker rat, a typical animal model of MS. In aorta from MS, we found following results, 1) Hypo-vasoconstriction induced by iNOS, 2) A decrease in thiol-antioxidants, Changes in arginine, methionine metabolisms (including a decrease in SAM level, a methyl donor) 3) Decreases in the methyl-arginine modifications on vascular proteins. Moreover, we found the involvement of reactive oxygen/nitrogen species and gas molecules with insulin signal in cultured endothelial cells.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (41 results)

All 2010 2009 2008 2007

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (23 results)

  • [Journal Article] Assessing a shift of glucose biotransformation by LC-MS/MS-based metabolome analysis in carbon monoxide-exposed cells.2010

    • Author(s)
      N. Takano, T. Yamamoto, T. Adachi, M Suematsu
    • Journal Title

      Adv Exp Med Biol 662

      Pages: 101-107

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] High plasma levels of matrix metalloproteinase-8 in patients with unstable angina.2009

    • Author(s)
      Momiyama Y, Ohmori R, Tanaka N, Kato R, Taniguchi H, Adachi T, Nakamura H, Ohsuzu F
    • Journal Title

      Atherosclerosis 209(1)

      Pages: 206-210

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term Carperitide Treatment Attenuates Left Ventricular Remodeling in Rats With Heart Failure After Autoimmune Myocarditis.2009

    • Author(s)
      Tanaka K, Ito M, Kodama M, Hoyano M, Kimura S, Mitsuma W, Hirono S, Adachi T, Watanabe K, Nakazawa M, Aizawa Y
    • Journal Title

      J Cardiovasc Pharmacol 54(3)

      Pages: 232-239

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative effects of olmesartan and azelnidipine on atrial structural remodeling in spontaneously hypertensive rats.2009

    • Author(s)
      Okamura K, Ito M, Tanaka K, Chinushi M, Adachi T, Mitsuma W, Hirono S, Nakazawa M, Kodama M, Aizawa Y
    • Journal Title

      Pharmacology 83(6)

      Pages: 360-366

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cosmic effects of rosuvastatin in COSMOS.2009

    • Author(s)
      Adachi T, Ohsuzu F
    • Journal Title

      Circ J 73(11)

      Pages: 2015-2016

    • NAID

      10025937055

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic Remodeling Induced by Mitochondrial Aldehyde Stress Stimulates Tolerance to Oxidative Stress in the Heart.2009

    • Author(s)
      Endo J, Sano M, Adachi T, Suematsu M, Ogawa S, Fukuda K., et. al
    • Journal Title

      Circ Res 105(11)

      Pages: 1118-1127

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cosmic effects of rosuvastatin in COSMOS.2009

    • Author(s)
      Adachi T, Ohsuzu F
    • Journal Title

      Circulation Journal 73

      Pages: 2015-2016

    • NAID

      10025937055

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic Remodeling Induced by Mitochondrial Aldehyde Stress Stimulates Tolerance to Oxidative Stress in the Heart2009

    • Author(s)
      Endo J, Sano M, Adachi T
    • Journal Title

      Circulation Research 105

      Pages: 1118-1127

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine triphosphate-sensitive potassium channels prevent extension of myocardial ischemia to subepicardium during hemorrhagic Shock.2008

    • Author(s)
      M Nakagawa, S. Hori, T. Adachi, K. Miyazaki, S. Inoue, M. Suzuki, H. Mori, H. Nakazawa, N. Aikawa, S. Ogawa
    • Journal Title

      Shock 30(2)(TA is the correspondence author)

      Pages: 178-183

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mercury chloride decreases the water permeability of aquaporine-4-reconstituted proteoliposomes.2008

    • Author(s)
      Y. Yukutake, S. Tsuji, Y. Hirano, T. Adachi, T. Takahashi, K. Fujihara, P. Agre, M. Yasui, M Suematsu
    • Journal Title

      Biol Cell 100(6)

      Pages: 355-363

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] High glucose oxidizes SERCA Cysteine-674 and prevents inhibition by nitric oxide of smooth muscle cell migration.2008

    • Author(s)
      X.Y. Tong, J. Ying, D. Pimentel, M.P. Trucillo, T. Adachi, R.A. Cohen
    • Journal Title

      J Mol Cell Cardiol 44(2)

      Pages: 361-369

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] High glucose oxidizes SERCA Cysteine-674 and prevents inhibition by nitric oxide of smooth muscle cell migration2008

    • Author(s)
      X. Y. Tong, J. Ying, D. Pimentel, M. P. Trucillo, T. Adachi, et al
    • Journal Title

      J Mol Cell Cardiol 44

      Pages: 361-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine triphosphate-sensitive potassium channels prevent exte nsion of myocardial ischemia to subepicardium during hemorrhagic Shock.2008

    • Author(s)
      M Nakagawa, S. Hori, T. Adachi, et al
    • Journal Title

      Shock 30

      Pages: 178-183

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mercury chloride decreases the water permeability of aquaporine-4-reconstituted proteoliposomes.2008

    • Author(s)
      Y. Yukutake, S. Tsuji, Y. Hirano, T. Adachi, et al
    • Journal Title

      Biol Cell 100

      Pages: 355-363

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] hiol oxidation in signal and response to stress: Detection and quantification of physiological and pathophysiological thiol modifications.2007

    • Author(s)
      J. Ying, N. Clavreul, M. Sethuraman, T. Adachi, R.A. Cohen
    • Journal Title

      Free Radical Biology & Medicine 43(8)

      Pages: 1099-1108

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeting NADPH oxidase: Ets-1 regulates p47phox.2007

    • Author(s)
      T. Adachi, M. Yamamoto, M Suematsu
    • Journal Title

      Circ Res 101

      Pages: 962-964

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dominant negative suppression of Rad leads to QT prolongation and causes ventricular arrhythmia via modulation of L-type Ca2+ channels in the heart.2007

    • Author(s)
      H. Yada, M. Murata, K. Shimoda, S. Yuasa, H. Kawaguchi, M. Ieda, T. Adachi, M. Murata, S. Ogawa, K. Fukuda
    • Journal Title

      Circ Res 101(1)

      Pages: 69-77

    • NAID

      10024457087

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeting NADPH oxidase: Ets-1 regulates p47phox2007

    • Author(s)
      Takeshi Adachi, et. al.
    • Journal Title

      Circulation Research 101

      Pages: 962-964

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Applications of metabolome analysis for cardiovascular diseases.2008

    • Author(s)
      T. Adachi
    • Organizer
      Global COE, Summer School Lecture
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2008-08-05
    • Related Report
      2009 Final Research Report
  • [Presentation] Vascular Effects of Carbon Monoxide2008

    • Author(s)
      T. Adachi, M Kaiimura, T Yamamoto, M Suematsu
    • Organizer
      Symposium in 8th Nitric Oxide Society
    • Place of Presentation
      仙台
    • Year and Date
      2008-05-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Global COE for humanoid metabolomics system Biology.2008

    • Author(s)
      T. Adachi, M Suematsu
    • Organizer
      GCOE symposium in UC Davis
    • Year and Date
      2008-04-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Hypomethylation of aortic proteins in metabolic syndrome2008

    • Author(s)
      T. Adachi
    • Organizer
      GCOE symposium in UC Davis
    • Year and Date
      2008-04-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Hypomethylation of aortic proteins in metabolic syndrome.2008

    • Author(s)
      T. Adachi
    • Organizer
      GCOE symposium
    • Place of Presentation
      uc Davis, CA, USA
    • Year and Date
      2008-04-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Vascular proteomics for protein-arginine-methylation in metabolic syndrome.2008

    • Author(s)
      T. Adachi, K. Ishiwata, M. Yamamoto, T. Yamamoto, M. Suematsu
    • Organizer
      FASEB, Experimental Biology
    • Year and Date
      2008-04-07
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Mechanism of vasodilatation with N-acethylcysteine and NaHS: Contribution of ATP-sensitive potassium channels.2008

    • Author(s)
      M. Yamamoto, T. Adachi, K. Ishiwata, S. Ikeda, Y. Hirayama, T. Soga, M. Suematsu
    • Organizer
      FASEB, Experimental Biology
    • Year and Date
      2008-04-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of the metabolic remodeling in liver by genetic mutation of ALDH2 in muscles.2008

    • Author(s)
      T. Hishiki, T. Adachi, M. Sano, T. Takayama, J. Endo, K. Fukuda, M. Suematsu
    • Organizer
      FASEB, Experimental Biology
    • Year and Date
      2008-04-06
    • Related Report
      2009 Final Research Report
  • [Presentation] Vascular Effects of Carbon Monoxide; Symposium in 8th Nitric Oxide Society of Japan2008

    • Author(s)
      T. Adachi, M Kajimura, T Yamamoto, M Suematsu
    • Organizer
      Application of metabolome for cardiovascular pathophysiology; CVI seminar, Department of Medicine
    • Place of Presentation
      Boston University
    • Year and Date
      2008-03-07
    • Related Report
      2009 Final Research Report
  • [Presentation] Post-translational oxidative modifications in redox-sensitive mechanism2008

    • Author(s)
      T. Adachi
    • Organizer
      第33回日本微小循環学会
    • Place of Presentation
      東京(邦文)
    • Year and Date
      2008-02-21
    • Related Report
      2009 Final Research Report
  • [Presentation] CO regulates the glucose metabolism via protein methylation.2008

    • Author(s)
      T. Yamamoto, N. Takano, T. Iwabuchi, T. Hishiki, T. Adachi, M. Suematsu
    • Organizer
      第33回日本微小循環学会
    • Place of Presentation
      東京(邦文)
    • Year and Date
      2008-02-21
    • Related Report
      2009 Final Research Report
  • [Presentation] 一酸化炭素(CO)による細胞内メチル化制御機構の解析第30回日本分子生物学会年会2007

    • Author(s)
      山本雄広、高野直治1. 2、菱木貴子、足立健、末松誠
    • Organizer
      第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] 一酸化炭素(CO)による糖代謝酵素のメチル化修飾の解析2007

    • Author(s)
      高野直治、山本雄広1、菱木貴子、足立健、末松誠
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Related Report
      2009 Final Research Report
  • [Presentation] Post-translational protein modifications modulated by NO and CO.2007

    • Author(s)
      T. Adachi, M. Suematsu
    • Organizer
      4th International Redox Meeting
    • Year and Date
      2007-11-01
    • Related Report
      2009 Final Research Report
  • [Presentation] CO regulates glucose metabolism via protein arginine methylation.2007

    • Author(s)
      N. Takano, T. Yamamoto, T. Iwabuchi, T. Adachi, M. Suematsu
    • Organizer
      Global COE Satellite Symposium "Metabolic Systems Biology and Liver Disease"
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-10-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 血管病における一酸化窒素・一酸化炭素による蛋白質翻訳後修飾2007

    • Author(s)
      足立健
    • Organizer
      第17回脳血管シンポジウム
    • Year and Date
      2007-09-01
    • Related Report
      2009 Final Research Report
  • [Presentation] 高温環境におけるラット大動脈のフェニレフリン収縮の検討2007

    • Author(s)
      上野浩一、鈴木昌、越智梢、山本美智子、足立健、堀進悟、相川直樹
    • Organizer
      臨床体温研究会
    • Year and Date
      2007-08-25
    • Related Report
      2009 Final Research Report
  • [Presentation] N-アセチル-システインおよびNaHSの血管拡張作用とその機序2007

    • Author(s)
      山本美智子、足立健、末松誠
    • Organizer
      第25回ヒト細胞学会
    • Year and Date
      2007-08-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Zucker Ratにおける血管内蛋白質メチル化の変化2007

    • Author(s)
      足立健、山本美智子、石渡恭子、末松誠
    • Organizer
      第25回ヒト細胞学会
    • Year and Date
      2007-08-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Zucker Ratにおける血管内蛋白質メチル化の変化2007

    • Author(s)
      足立 健, 他
    • Organizer
      第25目日本ヒト細胞学会
    • Place of Presentation
      東京都市センターホテル
    • Year and Date
      2007-08-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] N-アセチル-システインおよびNaHSの血管拡張作用とその機序2007

    • Author(s)
      山本 美智子, 他
    • Organizer
      第25回日本ヒト細胞学会
    • Place of Presentation
      東京都市センターホテル
    • Year and Date
      2007-08-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] COによる糖代謝酵素のメチル化修飾と代謝リモデリング2007

    • Author(s)
      高野直治、山本雄広、岩渕拓也、足立健、末松誠
    • Organizer
      第28回日本炎症・再生医学会
    • Place of Presentation
      東京
    • Year and Date
      2007-08-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Role of stress-inducible CO in regulation of protein methylation and metabolism.2007

    • Author(s)
      T. Yamamoto, N. Takano, T. Iwabuchi, T. Adachi, M. Suematsu
    • Organizer
      Nobel Forum Symposium for Global COE Program "Gas biology in medicine
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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