Role of stress-inducible NKG2D ligands in disease

• Project/Area Number: 19390107
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: KASAHARA Masanori Hokkaido University, 大学院・医学研究科, 教授 (30241318)
• Co-Investigator(Kenkyū-buntansha): TOMARU Utano 北海道大学, 大学院・医学研究科, 講師 (20360901) ; FUGO Kazunori 北海道大学, 大学院・医学研究科, 助教 (20431306)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
• Keywords: NKG2D / ナチュラルキラー細胞 / 活性化リガンド / H60

Research Abstract

NKG2Dリガンドはがん化やウイルス感染に伴って、細胞表面に発現される主要組織適合遺伝子複合体クラスI様分子であり、免疫系に異常細胞の存在を知らせる危険信号の働きをしている。本研究では、我々が同定した2個の新規リガンドを中心として、NKG2Dリガンドの構造、機能、病態における役割を解析した

Research Products

• [Journal Article] Exclusive expression of proteasome subunit β5t in the human thymic cortex (2009)
  • Author(s): Tomaru, U., Ishizu, A., Murata, S., Miyatake, Y., Suzuki, S., Takahashi, S., Kazamaki, T., Ohara, J., Baba, T., Iwasaki, S., Fugo, K., Otsuka, N., Tanaka, K. and Kasahara, M.
  • Journal Title: Blood doi : 10.1182/ blood-2008-11-187633
  • Peer Reviewed
• [Journal Article] Exclusive expression of proteasome subunit β5t in the human thymic cortex (2009)
  • Author(s): Tomaru, U.
  • Journal Title: Blood 113(In press)
  • Peer Reviewed
• [Journal Article] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D (2008)
  • Author(s): Takada, A., Yoshida, S., Kajikawa, M., Miyatake, Y., Tomaru, U., Sakai, M., Chiba, H., Maenaka, K., Kohda, D., Fugo, K. and Kasahara, M.
  • Journal Title: J. Immunol 180 Pages: 1678-1685
  • Peer Reviewed
• [Journal Article] and Ishizu, A.Rat CD4+/CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/per for in-dependent (2008)
  • Author(s): Baba, T., Iwasaki, S., Maruoka, T., Suzuki, A., Tomaru, U., Ikeda, H., Yoshiki, T., Kasahara, M
  • Journal Title: mechanism. J. Immunol 180 Pages: 2999-3006
  • Peer Reviewed
• [Journal Article] Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes. Nat (2008)
  • Author(s): Yashiroda, H., Mizushima, T., Okamoto, K., Kameyama T., Hayashi, H., Kishimoto, T., Niwa, S., Kasahara, M., Kurimoto, E., Sakata, E., Takagi, K., Suzuki, A., Hirano, Y., Murata, S., Kato, K., Yamane, T. and Tanaka, K
  • Journal Title: Struct. Mol. Biol 15 Pages: 228-236
  • Peer Reviewed
• [Journal Article] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D (2008)
  • Author(s): Takada, A.
  • Journal Title: J. Immunol. 180 Pages: 1678-1685
  • Peer Reviewed
• [Journal Article] Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes (2008)
  • Author(s): Yashiroda, H.
  • Journal Title: Nat. Struct. Mol. Biol. 15 Pages: 228-236
  • Peer Reviewed
• [Journal Article] Rat CD4+ / CD8+ macrophages kill tumor cells through an NKG2D-and granzyme / perforin-dependent mechanism (2008)
  • Author(s): Baba, T.
  • Journal Title: J. Immunol. 180 Pages: 2999-3006
  • Peer Reviewed
• [Journal Article] Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes. (2008)
  • Author(s): Yashiroda, H.
  • Journal Title: Nat. Struct. Mol. Biol. 15 Pages: 228-236
  • Peer Reviewed
• [Journal Article] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. (2008)
  • Author(s): Takada, A.
  • Journal Title: J. Immunol. 180 Pages: 1678-1685
  • Peer Reviewed
• [Journal Article] Rat CD4+/CD8+macrophages kill tumor cells through an NKG2D-and granzyme/perforin-dependent mechani (2008)
  • Author(s): Baba, T.
  • Journal Title: J. Immunol. 180 Pages: 2999-3006
  • Peer Reviewed
• [Journal Article] Structural diversity of the variable lymphocyte receptors (2007)
  • Author(s): Kim, H. M., Oh, S. C., Lim, K. J., Kasamatsu, J., Heo, J. Y., Lee, H., Yoo, O. J., Kasahara, M, and Li, J.-O
  • Journal Title: J. Biol. Chem 282 Pages: 6726-6732
  • Peer Reviewed
• [Journal Article] Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion (2007)
  • Author(s): Busso, D., Goldweic, N. M., Hayashi, M., Kasahara, M. and Cuasnicu, P. S.
  • Journal Title: Biol. Reprod 76 Pages: 701-708
  • Peer Reviewed
• [Journal Article] Enhanced production of p24 Gag protein in HIV-1-infected rat cells fused with uninfected human cells. Exp (2007)
  • Author(s): Chen, J., Zhao, X., Lai, Y., Suzuki, A., Tomaru, U., Ishizu, A., Takada, A., Ikeda, H., Kasahara, M. and Yoshiki, T
  • Journal Title: Mol. Pathol 83 Pages: 125-130
  • Peer Reviewed
• [Journal Article] The 2R hypothesis : An update (2007)
  • Author(s): Kasahara, M
  • Journal Title: Curr. Opin. Immunol 19 Pages: 547-552
  • Peer Reviewed
• [Journal Article] Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. (2007)
  • Author(s): Busso, D.
  • Journal Title: Biol. Reprod. 76 Pages: 701-708
  • Peer Reviewed
• [Journal Article] Two variable lymphocyte receptor genes of the inshore hagfish are located far apart on the same chromosome. (2007)
  • Author(s): Kasamatsu, J.
  • Journal Title: Immunogenetics 59 Pages: 329-331
  • Peer Reviewed
• [Journal Article] Enhanced production of p24 Gag protein in HIV-1-infected rat cells fused with uninfected human cells. (2007)
  • Author(s): Chen, J.
  • Journal Title: Exp. Mol. Pathol. 83 Pages: 125-130
  • Peer Reviewed
• [Journal Article] The 2R hypothesis: An update. (2007)
  • Author(s): Kasahara, M.
  • Journal Title: Curr. Opin. Immunol. 19 Pages: 547-552
  • Peer Reviewed
• [Journal Article] A degenerate ParaHox gene cluster in a degenerate vertebrate. (2007)
  • Author(s): Furlong, R. F.
  • Journal Title: Mol. Biol. Evol. 24 Pages: 2681-2686
  • Peer Reviewed
• [Presentation] Role of mouse NKG2D ligand H60c in skin immunity (2008)
  • Author(s): Yoshida, S., Fugo, K., Kasamatsu, J., Sarada, Y., Otsuka, N., and Kasahara, M.
  • Organizer: 第38回日本免疫学会総会, 学術集会
  • Place of Presentation: 京都
• [Presentation] Crystal structure of nonclassical MHC class I molecule MILL2 (2008)
  • Author(s): Kajikawa, M., Ose, T., Kasahara, M. and Maenaka, K.
  • Organizer: 第38回日本免疫学会総会, 学術集会
  • Place of Presentation: 京都
• [Presentation] The NKG2D ligand family of the mouse : A "danger signal" alerting the immune system. Regulation in Innate Immunity : From Recognition Molecules to Antimicrobial Peptides (Organizers : Ayabe, T., Kawano, K. and Matsushima, N (2008)
  • Author(s): Kasahara, M., Yoshida, S., Kajikawa, M., Sarada, Y., Kasamatsu, J., Otsuka, N. and Fugo, K.
  • Organizer: October
  • Place of Presentation: Sapporo
• [Presentation] NKG2D ligand H60b,H60cの機能解析 (2008)
  • Author(s): 吉田 繁、富居一範、大塚紀幸、外丸詩野、笠原正典
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Presentation] 胸腺プロテアソームの発現とT細胞分化に関する検討 (2008)
  • Author(s): 外丸詩野、石津明洋、宮武由甲子、高橋里美、小原次郎、村田茂穂、田中啓二、笠原正典
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Presentation] ヒト組織におけるプロテアソームサブユニットβ5t発現に関する検討 (2008)
  • Author(s): 外丸詩野、石津明洋、宮武由甲子、鈴木小百合、風巻 拓、村田茂穂、田中啓二、笠原正典
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
• [Presentation] 自己免疫疾患モデルマウスにおけるプロテアソームサブユニットβ5t発現に関する検討 (2008)
  • Author(s): 風巻 拓、外丸詩野、石津明洋、鈴木小百合、笠原正典
  • Place of Presentation: 金沢
• [Presentation] Role of mouse NKG2D ligand H60c in skin immunity (2008)
  • Author(s): Yoshida, S.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都(国立京都国際会館)
• [Presentation] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D (2007)
  • Author(s): Fugo, K., Yoshida, S., Takada, A., Kajikawa, M., Miyatake, Y., Tomaru, U., Chiba, H., Maenaka, K., Kohda, D. and Kasahara, M.
  • Organizer: 第37回日本免疫学会総会, 学術集会
  • Place of Presentation: 東京
• [Presentation] 胸腺プロテアソームのT細胞分化、免疫制御における役割 (2007)
  • Author(s): 外丸詩野、石津明洋、宮武由甲子、村田茂穂、田中啓二、笠原正典
  • Organizer: 第37回日本免疫学会総会, 学術集会
  • Place of Presentation: 東京
• [Presentation] CD4/CD8 double positiveマクロファージによる抗腫瘍メカニズムの解明 (2007)
  • Author(s): 馬場智久、岩崎沙理、石津明洋、鈴木 昭、外丸詩野、池田 仁、吉木 敬、笠原正典
  • Organizer: 第96回日本病理学会総会, 第96回日本病理学会総会
  • Place of Presentation: 大阪
• [Presentation] 生殖細胞特異的に発現する新規MHC class I様分子の解析 (2007)
  • Author(s): 富居一範、吉田 繁、宮武由甲子、高田明生、鈴木 昭、石津明洋、外丸詩野、笠原正典
  • Organizer: 第96回日本病理学会総会, 第96回日本病理学会総会
  • Place of Presentation: 大阪
• [Presentation] 新しいマウスNKG2D ligandの同定と解析 (2007)
  • Author(s): 吉田 繁、富居一範、高田明生、宮武由甲子、鈴木 昭、石津明洋、外丸詩野、笠原正典
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
• [Presentation] 潰瘍性大腸炎における末梢血中NKG2A陽性T細胞の減少と病態への関与 (2007)
  • Author(s): 桂田武彦、外丸詩野、古川 滋、武田宏司、田中淳司、浅香正博、鈴木 昭、石津明洋、笠原正典
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
• [Presentation] Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities (2007)
  • Author(s): Yoshida, S.
  • Organizer: 第66回日本癌学会学術集会
  • Place of Presentation: 横浜(パシフィコ横浜)
• [Book] Chichester, UK, DOI (2007)
  • Author(s): Kasahara, M.
  • Publisher: Polyploid origin of the human genome. In : D. N. Cooper and H. Kehrer-Sawatzki(eds.) : Encyclopedia of Life Sciences, John Wiley & Sons