The extracellular microenvironment and its signal regulations operated by proteoglycans

• Project/Area Number: 19390116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Aichi Medical University
• Principal Investigator: WATANABE Hideto Aichi Medical University, 分子医科学研究所, 教授 (90240514)
• Co-Investigator(Kenkyū-buntansha): HATANO Sonoko 愛知医科大学, 分子医科学研究所, 助教 (40434625)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 糖鎖 / 細胞・組織 / プロテオグリカン / 細胞外マトリックス / シグナル伝達 / 遺伝子改変マウス / バーシカン / フロテオグリカン / 軟骨分化 / TGFbeta

Research Abstract

To understand in vivo function of versican, a large proteoglycan in the extracellular matrix, we generated analyzed its conditional knockout mice. Through their analyses, we found that versican regulates BMP- and TGFβ-mediated signaling and plays crucial roles in several steps of cardiac development, chondrogenesis, and joint morphogenesis.

Research Products

• [Journal Article] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis. (2010)
  • Author(s): Choocheep K, Hatano S, Takagi H, Watanabe H, Kimata K, Kongtawelert P, Watanabe H
  • Journal Title: J Biol Chem (in press)
  • Peer Reviewed
• [Journal Article] Crucial role of vinexin for keratinocyte migration and epidermal wound healing in vivo. (2010)
  • Author(s): Ito T, Yamashita H, Uekawa N, Umemoto T, Motoyoshi S, Imai H, Takahashi K, Watanabe H, Yamada M, Ueda K
  • Journal Title: Exp Cell Res 316 Pages: 1728-1738
  • Peer Reviewed
• [Journal Article] The Glucuronyl transferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA ; the essential enzymes for production of K5 polysaccharide, N-acetylheparosan. (2010)
  • Author(s): Sugiura N, Baba Y, Kawaguchi Y, Iwatani T, Suzuki K, Kusakabe T, Yamagishi, K, Kimata K, Kakuta Y, Watanabe H
  • Journal Title: J Biol Chem 285 Pages: 1597-1606
  • Peer Reviewed
• [Journal Article] Versican facilitates chondorcyte differentiation and regulates joint Morphogenesis (2010)
  • Author(s): Chooceep K, et al.
  • Journal Title: J Biol Chem (In press)
  • Peer Reviewed
• [Journal Article] Crucial role of vinexin for keratinocyte migration and epidermal wound healing in vivo (2010)
  • Author(s): Ito T, et al.
  • Journal Title: Exp Cell Res (In press)
  • Peer Reviewed
• [Journal Article] The Glucuronyl transferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA ; the essential enzymes for production of K5 polysaccharide, N-acetylheparosan (2010)
  • Author(s): Sugiura N, et al.
  • Journal Title: J Biol Chem 285 Pages: 1597-1606
  • Peer Reviewed
• [Journal Article] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis (2010)
  • Author(s): Choocheep K, et al.
  • Journal Title: J Biol Chem (In press)
  • Peer Reviewed
• [Journal Article] Glucuronyliransferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA : KfiC and KfiA are essential enzymes for production of K5 polysaccharide, N-acetylheparosan (2010)
  • Author(s): Sugiura, N., et al.
  • Journal Title: J Biol Chem 285 Pages: 1597-1606
  • Peer Reviewed
• [Journal Article] Ultrastructural immunolocalization of a cartilage-specific proteoglycan, aggrecan, in salivary pleomorphic adenomas. (2009)
  • Author(s): Muramatsu K, Kusafuka K, Watanabe H, Mochizuki T, Nakajima T
  • Journal Title: Med Mol Morphol 42 Pages: 47-54
  • NAID: 10025859759
  • Peer Reviewed
• [Journal Article] Alteration of chondroitin sulfate composition on proteoglycan produced by knockin mouse embryonic fibroblasts whose versican lacks the A-subdomain. (2009)
  • Author(s): Suwan K, Hatano S, Kongtawelert P, Pothachaloen P, Watanabe H
  • Journal Title: Ups J Med Sci 114 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Versican/PG-M assembles hyaluronan into extracellular matrix and inhibits CD44-mediated signaling toward premature senescence in embryonic fibroblasts. (2009)
  • Author(s): Suwan K, Choocheep K, Hatano S, Kongtawelert P, Kimata K, Watanabe H
  • Journal Title: J Biol Chem 284 Pages: 8596-8604
  • Peer Reviewed
• [Journal Article] ersican/PG-M assembles hyaluronan into extracellular matrix and inhibits CD44-mediated signaling toward premature senescence in embryonic fibroblasts (2009)
  • Author(s): Suwan, K
  • Journal Title: J Biol Chem 284 Pages: 8596-8604
  • Peer Reviewed
• [Journal Article] Cartilaginous features in matrix-producing carcinoma of the breast : four cases report with histochemical and immunohistolochemical analysis of matrix molecules. (2008)
  • Author(s): Kusafuka K, Muramatsu K, Kasami M, Kuriki K, Hirobe K, Hayashi I, Watanabe H, Hiraki Y, Shukunami C, Mochizuki T, Kameya T
  • Journal Title: Modern Pathol 21 Pages: 1282-1292
  • Peer Reviewed
• [Journal Article] Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. (2008)
  • Author(s): Sugiura N, Shimokata S, Minamisawa T, Hirabayashi J, Kimata K, Watanabe H
  • Journal Title: Glycoconj J 25 Pages: 521-530
  • Peer Reviewed
• [Journal Article] Equivalent involvement of inter-a-trypsin inhibitor heavy chain isoforms in forming covalent complexes with hyaluronan. (2008)
  • Author(s): Zhu L, Zhuo L, Watanabe H, Kimata K
  • Journal Title: Conn Tiss Res 49 Pages: 48-55
  • Peer Reviewed
• [Journal Article] Splicing factor 3b subunit 4 binds BMPR-IA and inhibits osteochondral cell differentiation. (2007)
  • Author(s): Watanabe H, Shionyu M, Kimura T, Kimata K, Watanabe H
  • Journal Title: J Biol Chem 282 Pages: 20728-20738
  • Peer Reviewed
• [Journal Article] MS analysis of chondroitin polymerization : Effects of Mn^<2+> ions on the stability of UDP-sugars and chondroitin synthesis. (2007)
  • Author(s): Sugiura N, Shimokata S, Watanabe H, Kimata K
  • Journal Title: Anal Biochem 365 Pages: 62-73
  • Peer Reviewed
• [Journal Article] Minute pleomorphic adenoma of the submandibular gland in the patient with oral malignancy : a report of two cases with histological and histochemical examination. (2007)
  • Author(s): Kusafuka K, Watanabe H, Kimata K, Hiraki Y, Shukunami C, Kameya T
  • Journal Title: Histopathology 51 Pages: 258-261
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage. (2007)
  • Author(s): Sakai K, Kimata K, Sato T, Gotoh M, Narimatsu H, Shinomiya K, Watanabe H
  • Journal Title: J Biol Chem 282 Pages: 4152-4161
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage. (2007)
  • Author(s): Sakai, K., et. al.
  • Journal Title: J Biol Chem 282 Pages: 4152-4161
  • Peer Reviewed
• [Journal Article] Splicing factor 3b subunit 4 binds BMPR-IA and inhibits osteochondral cell differentiation. (2007)
  • Author(s): Watanabe, H., et. al.
  • Journal Title: J Biol Chem 282 Pages: 20728-20738
  • Peer Reviewed
• [Presentation] Versican/PG-M assembles hyaluronan into the extracellular matrix and inhibit CD44-mediated signaling toward premature senescence in embryonic fibroblasts (2009)
  • Author(s): Watanabe H, Suwan K, Choocheep K, Hatano S, Kongtawelert P, Kimata K
  • Organizer: 8^<th> Panpacific connective Tissue Societies Symposium
  • Place of Presentation: 湘南
  • Year and Date: 2009-06-05
• [Presentation] Versican/PG-M assembles hyaluronan into the extracellular matrix and inhibit CD44-mediated signaling toward premature senescence in embryonic fibroblasts (2009)
  • Author(s): Watanabe H, Suwan K, Choocheep K, Hatano S, Kongtawelert P, Kimata K
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] バーシカン/PG-Mの多彩な生体内機能BMB2008 (2008)
  • Author(s): 渡辺秀人
  • Organizer: 第31回分子生物学会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] In vivo function of versican/PG-M : analysis of knockin mice (2008)
  • Author(s): Watanabe H
  • Organizer: 13^<th> Gordon Research Conference on Proteoglycans
  • Place of Presentation: Andover, NH, U.S.A.
  • Year and Date: 2008-07-08
• [Presentation] コンドロイチン硫酸:多様な糖鎖構造と生合成機構 (2008)
  • Author(s): 渡辺秀人
  • Organizer: 第40回日本結合組織学会学術大会・第55回マトリックス研究会大会合同学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-30
• [Presentation] CSGalNAcT-1は軟骨組織におけるコンドロイチン硫酸合成に主要な役割を果たす (2008)
  • Author(s): 坂井顕一郎、木全弘治、佐藤隆、後藤雅式、成松久、四宮謙一、渡辺秀人
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-22
• [Presentation] 軟骨のプロテオグリカン会合体 (2008)
  • Author(s): 渡辺秀人
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-21
• [Presentation] In vivo function of versican : analysis of knockin mice (2007)
  • Author(s): Watanabe H
  • Organizer: Panpacific Connective Tissue Society Symposium
  • Place of Presentation: ケアンズ、オーストラリア
  • Year and Date: 2007-10-30
• [Presentation] In vivo funcion of versican: analysis of knockin mice (2007)
  • Author(s): Hideto Watanabe
  • Organizer: 7th Pan Pacific Connective Tissue Society Symposium
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2007-10-29
• [Presentation] 生体内におけるバーシカン/PG-Mの機能 (2007)
  • Author(s): 渡辺 秀人
  • Organizer: 第28回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-03
• [Presentation] 生体内におけるバーシカン/PG-Mの機能 (2007)
  • Author(s): 渡辺秀人
  • Organizer: 日本炎症再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-02
• [Book] Comprehensive Glycoscience, Volume IV(Ed., Kamerling, J. P., et al.)(Knockout mice and proteoglycans.) (2007)
  • Author(s): Kimata K, Habuchi O, Habuchi H, Watanabe H
  • Publisher: Elsevier
• [Book] Comprehensive Glycoscience, Volume IV, (2007)
  • Author(s): Kimata, K., et. al.
  • Publisher: Elsevier
• [Remarks]
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