Studies on the mechanism of maturation and egress of herpes simplex virus

• Project/Area Number: 19390132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Nagoya University
• Principal Investigator: NISHIYAMA Yukihiro Nagoya University, 大学院・医学系研究科, 教授 (60115615)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2007: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 単純ヘルペスウイルス / 増殖機構 / 核マトリックス / 核外輸送

Research Abstract

ヘルペスウイルスのカプシドは核内で形成された後、核外へと輸送されてポストゴルジの小胞で出芽しエンベロープを獲得する。これは他の動物ウイルスにはないユニークな増殖様式であり、その分子機構には不明な点が多い。本研究では、ヘルペスウイルスのプロトタイプである単純ヘルペスウイルスの成熟、細胞外排出機構に関わるウイルス側、細胞側の諸要因について解析した。その結果、HSVの感染によって核骨格構造にダイナミックな変化が誘導されること、ウイルス粒子の成熟・排出過程に関わると考えられるUL14、UL56、US3などの機能と役割について新たな知見を得た。

Research Products

• [Journal Article] Detection of cytomegalovirus DNA in preserved umbilical cords from patients with sensorineural hearing loss. (2009)
  • Author(s): Mizuno T, Sugiura S, Kimura H, et al.
  • Journal Title: Eur Arch Otorhinolaryngol 266 Pages: 351-355
  • Peer Reviewed
• [Journal Article] Herpes Simplex Virus Induces Extensive Modification and Dynamic Relocalisation of the Nuclear Mitotic Apparatus Protein (NuMA) in Interphase Cells (2008)
  • Author(s): Yamauchi, Y., Kiriyama, K., Kimura, H. and Nishiyama, Y.
  • Journal Title: Journal of Cell Science 121 Pages: 2087-2096
  • Peer Reviewed
• [Journal Article] Identification of a physiological phosphorylation site of the Herpes simplex virus 1-encoded protein kinase Us3 which regulates its optimal catalytic activity in vitro and influences its function in infected cells (2008)
  • Author(s): Kato, A., Tanaka, M., Yamamoto, M., Asai, R., Sata, T., Nishiyama, Y. and Kawaguchi, Y.
  • Journal Title: Journal of Virology 82 Pages: 6172-6189
  • Peer Reviewed
• [Journal Article] Herpes Simplex Virus Type 2 UL56 Interacts with the Ubiquitin Ligase Nedd4 and Increases its Ubiquitination (2008)
  • Author(s): Ushijima Y, Koshizuka T, Goshima F, Kimura H, Nishiyama Y.
  • Journal Title: Journal of Virology 82 Pages: 5220-5233
  • Peer Reviewed
• [Journal Article] The UL14 Tegument Protein of Herpes Simplex Virus Type 1 (HSV 1) is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids (2008)
  • Author(s): Yamauchi Y, Kiriyama K, Kubota N, Kimura H, Usukura J, Nishiyama Y.
  • Journal Title: Journal of Virology 82 Pages: 1094-1106
  • Peer Reviewed
• [Journal Article] A regulatory role of interleukin 15 in wound healing and mucosal infection in mice (2008)
  • Author(s): Kagimoto, T., Yamada, H., Ishikawa, T., Maeda, N., Goshima, F., Nishiyama, Y., Furue, M., Yoshikai, Y.
  • Journal Title: Journal of Leukocyte Biology 83 Pages: 165-172
  • Peer Reviewed
• [Journal Article] Microarray analysis of transcriptional responses to infection by herpes simplex virus types 1 and 2 and their US3-deficient mutants (2008)
  • Author(s): Kamakura, M., Nawa, A., Ushijima, Y., Goshima, F., Kawaguchi, Y., Kikkawa, F., Nishiyama, Y.
  • Journal Title: Microbes and Infection 10 Pages: 405-413
  • Peer Reviewed
• [Journal Article] Oncolytic viral therapy for malignant melanoma with herpes simplex virus type 1 mutant HF10 (2008)
  • Author(s): Watanabe, D., Goshima, F., Mori, I., Tanada, Y., Matsumoto, Y., Nishiyama, Y.
  • Journal Title: Journal of Dermatological Science 50 Pages: 185-196
  • Peer Reviewed
• [Journal Article] A regulatory role of interleukin 15 in wound healing and mucosal infection in mice. (2008)
  • Author(s): Kagimoto, T., Yamada, H., Ishikawa, T, et al.
  • Journal Title: Journal of Leukocyte Biology 83 Pages: 165-172
  • Peer Reviewed
• [Journal Article] One-Step multiplex real-time PCR assay to analyze the latency patterns of Epstein-Barr virus infection. (2008)
  • Author(s): Kubota, N., Wada, K., Ito, Y., et al.
  • Journal Title: Journal of Virological Methods 147 Pages: 26-36
  • Peer Reviewed
• [Journal Article] The UL14 Tegument Protein of Herpes Simplex Virus Type 1 (HSV-1) is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids. (2008)
  • Author(s): Yamauchi Y, Kiriyama K, Kubota N, et al.
  • Journal Title: Journal of Virology 82 Pages: 1094-1106
  • Peer Reviewed
• [Journal Article] Gene directed enzyme prodrug therapy for ovarian cancer. - Could GDPET become a promising treatment against ovarian cancer? (2008)
  • Author(s): Nawa, A., Tanino, T., Luo, C, et al.
  • Journal Title: Anti-Cancer Agents in Medical Chemisyry 8 Pages: 232-239
  • Peer Reviewed
• [Journal Article] Microarray analysis of transcriptional responses to infection by herpessimplex virus types 1 and 2 and their US3-deficient mutants. (2008)
  • Author(s): Kamakura, M., Nawa, A., Ushijima. Y., et al.
  • Journal Title: Microbes and Infection 10 Pages: 405-413
  • Peer Reviewed
• [Journal Article] Oncolytic viral therapy for malignant melanoma with herpes simplex virus type 1 mutant HF10. (2008)
  • Author(s): Watanabe, D., Goshima, F., Mori, I., et al.
  • Journal Title: Journal of Dermatological Science 50 Pages: 185-196
  • Peer Reviewed
• [Journal Article] Herpes Simplex Virus Type 2 UL56 Interacts with the Ubiquitin Ligase Nedd4 and Increases its Ubiquitination. (2008)
  • Author(s): Ushijima Y, Koshizuka T, Goshima F., et al.
  • Journal Title: Journal of Virology 82 Pages: 5220-5223
  • Peer Reviewed
• [Journal Article] Herpes Simplex Virus Induces Extensive Modification and Dynamic Relocalisation of the Nuclear Mitotic Apparatus Protein (NuMA) in Interphase Cells (2008)
  • Author(s): Yamauchi, Y., Kiriyama, K., Kimura, H., Nishiyama, Y.
  • Journal Title: Journal of Cell Science 121 Pages: 2087-2096
  • Peer Reviewed
• [Journal Article] Identification of a physiological phosphorylation site of the Herpes simplex virus 1-encoded protein kinase Us3 which regulates its optimal catalytic activity in vitro and influences its function in infected cells. (2008)
  • Author(s): Kato, A., Tanaka, M., Yamamoto, M., et al.
  • Journal Title: Journal of Virology 82 Pages: 6172-6189
  • Peer Reviewed
• [Journal Article] Non-engineered, naturally oncolytic herpes simplex virus HSV1 HF-10 : applications for cancer gene therapy. (2008)
  • Author(s): Nawa A, Luo C, Zhang L, et al.
  • Journal Title: Curr Gene Therapy 8 Pages: 208-221
  • Peer Reviewed
• [Journal Article] Measuring Epstein-Barr virus (EBV) load : the significance and applic ation for each EBV-associated disease. (2008)
  • Author(s): Kimura H, Ito Y, Suzuki R, Nishiyama Y.
  • Journal Title: Reviews in Medical Virology 18 Pages: 305-319
  • Peer Reviewed
• [Journal Article] Conversion from mycophenolate mofetil to mizoribine for patients with positive polyomavirus type BK in urine. (2008)
  • Author(s): Funahashi Y, Hattori R, Kinukawa T, et al.
  • Journal Title: Transplant Proc 40 Pages: 2268-2270
  • Peer Reviewed
• [Journal Article] The UL14 Tegument Protein of Herpes Simplex Virus Type 1 (HSV-1) is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids. (2008)
  • Author(s): Yamauchi Y, Kiriyama K, Kubota N, et. al.
  • Journal Title: Journal of Virology 82 Pages: 1094-1106
  • Peer Reviewed
• [Journal Article] A regulatory role of interleukin 15 in wound healing and mucosal in 1. (2008)
  • Author(s): Kagimoto, T., Yamada, H., Ishikawa, T., et. al.
  • Journal Title: Journal of Leukocyte Biology 83 Pages: 166-172
  • Peer Reviewed
• [Journal Article] One-step multiplex real-time PCR assay to analyse the latency patterns of Epstein-Barrvirus infection. (2008)
  • Author(s): Kubota, N., Wada, K., Ito, Y., et. al.
  • Journal Title: Journal of Virological Methods 147 Pages: 26-36
  • Peer Reviewed
• [Journal Article] Replication-competent, oncolytic herpes simplex virus type 1 mutants induce a bystander effect following ganciclovir treatment (2007)
  • Author(s): Luo, C., Mori, I., Goshima, F., Ushijima, Y., Nawa, A., Kimura, H., Nishiyama, Y
  • Journal Title: Journal of Gene Medicine 9 Pages: 875-883
  • Peer Reviewed
• [Journal Article] Herpes simplex virus protein UL11 but not UL51 is associated with lipid rafts (2007)
  • Author(s): Koshizuka, T., Kawaguchi, Y., Nozawa, N., Mori, I. and Nishiyama, Y.
  • Journal Title: Virus Genes 35 Pages: 571-575
  • Peer Reviewed
• [Journal Article] Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus (2007)
  • Author(s): Ushijima Y, Luo C, Goshima F, Yamauchi Y, Kimura H, Nishiyama Y.
  • Journal Title: Microb Infect 9(2) Pages: 142-149
  • Peer Reviewed
• [Journal Article] Oncolytic Virotherapy with an HSV amplicon vector expressing granulocyte-macrophage colony-stimulating factor using the replication-competent HSV type I mutant HF10 as a helper (2007)
  • Author(s): Kohno, S., Luo, C., Nawa, A., Fujimoto, Y., Waranabe, D., Goshima, F., Tsurumi, T. and Nishiyama, Y.
  • Journal Title: Cancer Gene Therapy 14 Pages: 918-926
  • Peer Reviewed
• [Journal Article] Suitability of a US3-inactivated HSV mutant(L1BR1) as an oncolytic virus for pancreatic cancer therpay (2007)
  • Author(s): Kasuya, H.,Nishiyama, Y., Nomoto, S., Goshima, F., Takeda, S., Watanabe, I., Nomura, N., Shikano, T., Fujii, T., Kanazumi, N. and Nakao, A
  • Journal Title: Cancer Gene Therapy 14 Pages: 533-542
  • Peer Reviewed
• [Journal Article] Intercellular trafficking and cytotoxicity of recombinant HSV-1 thymidine kinase fused with HSV-2 US11 RXP repeat peptide (2007)
  • Author(s): Luo, C., Nawa, A., Yamauchi, Y., Kohno, S., Ushijima, Y., Goshima, F. and Nishiyama, Y.
  • Journal Title: Virus Genes 34 Pages: 263-272
  • Peer Reviewed
• [Journal Article] Herpes simplex virus protein UL11 but not UL51 is associated with lipid rafts. (2007)
  • Author(s): Koshizuka, T., Kawaguchi, Y., Nozawa, N., et. al.
  • Journal Title: Virus Genes 35 Pages: 571-575
  • Peer Reviewed
• [Journal Article] Oncolytic Virotherapy with an HSV amplicon vector expressing granulocyte-macrophage colony-stimulating factor using the replication-competent HSV type Imutant HF10 as a helper (2007)
  • Author(s): Kohno, S., Luo, C., Nawa, A., et. al.
  • Journal Title: Cancer Gene Therapy 14 Pages: 918-926
  • Peer Reviewed
• [Journal Article] Replication-competent, oncolytic herpes simplex virus type 1 mutants induce a bystander effect following ganciclovir treatment. (2007)
  • Author(s): Luo, C., Mori, I., Goshima, F., et. al.
  • Journal Title: Journal of Gene Medicine 6 Pages: 875-883
  • Peer Reviewed
• [Journal Article] Suitability of a US3-inactivated HSV mutant (LIBRI) as an oncolytic-virus for pancreatic cancer therpay. (2007)
  • Author(s): Kasuya, H., Nishiyama, Y., Nomoto, S., et. al.
  • Journal Title: Cancer Gene Therapy 14 Pages: 533-542
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/virus