Analysis of host factors involved in the infection and replication of hepatitis C virus

• Project/Area Number: 19390133
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: MATSUURA Yoshiharu Osaka University, 微生物病研究所, 教授 (50157252)
• Co-Investigator(Kenkyū-buntansha): ABE Takayuki 大阪大学, 微生物病研究所, 特任研究員 (90403203)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: HCV,感染 / 集合 / 宿主因子 / 病原性 / HCV / 感染 / 複製

Research Abstract

C型肝炎ウイルス(HCV)の感染機構を解析するために、HCVのエンベロープ遺伝子を組み込んだ、自立増殖可能な組換え水疱性口内炎ウイルスの作製に成功した。また、HCVの成熟、複製、そして病原性発現に関与する宿主因子を複数同定し、これらの宿主因子を標的とした、耐性株の出現しにくい、新規C型肝炎治療薬開発の可能性を提示した。

Research Products

• [Journal Article] Human butyrate-induced transcript 1 interacts with hepatitis C virus NS5A and regulates viral replication (2008)
  • Author(s): Taguwa S., Okamoto T., Abe T., Mori Y., Suzuki T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol 82 Pages: 2631-2641
• [Journal Article] A single amino acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication (2008)
  • Author(s): Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol 82 Pages: 3480-2489
• [Journal Article] A critical role of virion-associated cholesterol and sphingolipid in hepatitis C virus infection (2008)
  • Author(s): Aizaki H., Morikawa K., Fukasawa M., Hara H., Inoue Y., Tani H., Saito K., Hanada K., Matsuura Y., Lai M.M.C., Miyamura T., Wakita T., and Suzuki T.
  • Journal Title: J. Virol 82 Pages: 5715-5724
• [Journal Article] Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles (2008)
  • Author(s): Masaki T., Suzuki R., Murakami K., Aizaki H., Ishii K., Murayama A., Date T., Matsuura Y., Miyamura T., Wakita T., and Suzuki T.
  • Journal Title: J. Virol 82 Pages: 7964-7976
• [Journal Article] Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation (2008)
  • Author(s): Okamoto K., Mori Y., Komoda Y., Okamoto T., Okochi M., Takeda M., Suzuki T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol 82 Pages: 8349-8361
• [Journal Article] Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles. (2008)
  • Author(s): Masaki T.
  • Journal Title: J. Virol. 82 Pages: 7964-7976
  • Peer Reviewed
• [Journal Article] A crtical role of virion-associated cholesterol and sphingoli pid in hepatitis C virus infection. (2008)
  • Author(s): Aizaki H.
  • Journal Title: J. Virol. 82 Pages: 5715-5724
  • Peer Reviewed
• [Journal Article] A single amino acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. (2008)
  • Author(s): Okamoto T.
  • Journal Title: J. Virol. 82 Pages: 3480-3489
  • Peer Reviewed
• [Journal Article] Human butyrate-induced transcript 1 interacts with hepatitis Cvirus NS5A and regulates viral replication. (2008)
  • Author(s): Taguwa S.
  • Journal Title: J. Virol. 82 Pages: 2631-2641
  • Peer Reviewed
• [Journal Article] Host factors involved in the replication of hepatitis C virus (2007)
  • Author(s): Moriishi K., and Matsuura Y.
  • Journal Title: Rev. Med. Virol 17 Pages: 343-354
• [Journal Article] Enhanced TLR-mediated NF-IL6 dependent gene expression by Trib1 deficiency. (2007)
  • Author(s): Yamamoto M., Uematsu S., Okamoto T., Matsuura Y., Sato S., Kumar H., Satoh T., Saitoh T., Takeda K., Ishii K.J., Takeuchi O., Kawai T., and Akira S.
  • Journal Title: J. Exp. Med. 207 Pages: 2233-2239
• [Journal Article] The E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis C virus core protein (2007)
  • Author(s): Shirakura M., Murakami K., Ichimura T., Suzuki R., Shimoji T., Fukuda K., Abe K., Sato S., Fukasawa M., Yamakawa Y., Nishijima M., Moriishi K., Matsuura Y., Wakita T., Suzuki T., Howley P.M., Miyamura T., and Shoji I.
  • Journal Title: J. Virol 81 Pages: 1174-1185
• [Journal Article] Involvement of PA28 r -dependent pathway in insulin resistance induced by hepatitis C virus core protein (2007)
  • Author(s): Miyamoto H., Moriishi K., Moriya K., Murata S., Tanaka K., Suzuki T., Miyamura T., Koike K., and Matsuura Y.
  • Journal Title: J. Virol 81 Pages: 1727-1735
• [Journal Article] Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins (2007)
  • Author(s): Tani H., Komoda Y., Matsuo E., Suzuki K., Hamamoto I., Yamashita T., Moriishi K., Fujiyama K., Kanto T., Hayashi N., Owsianka A., Patel A.H., Whitt M.A., and Matsuura Y.
  • Journal Title: J. Virol 81 Pages: 8601-8612
• [Journal Article] Processing of Capsid Protein by Cathepsin L Plays a Crucial Role in Replication of the Japanese Encephalitis Virus in Neural and Macrophage Cells. (2007)
  • Author(s): Mori Y., Yamashita T., Tanaka Y., Tsuda Y., Abe T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol 81 Pages: 8477-8487
• [Journal Article] Hepatitis C Virus Nonstructural Protein 5A Modulates TLR-MyD88-Dependent Signaling Pathway in the Macrophage Cell Lines (2007)
  • Author(s): Abe T., Kaname Y., Hamamoto I., Tsuda Y., Wen X., Taguwa S., Moriishi K., Takeuchi O., Kawai T., Kanto T., Hayashi N., Akira S., and Matsuura Y.
  • Journal Title: J. Virol 81 Pages: 8953-8966
• [Journal Article] Critical role of PA28 r in hepatitis C virus-associated steatogenesis and hepatocarcinogenesis (2007)
  • Author(s): Moriishi K., Mochizuki R., Moriya K., Miyamoto H., Mori Y., Abe T., Murata S., Tanaka K., Miyamura T., Suzuki T., Koike K., and Matsuura Y.
  • Journal Title: PNAS 104 Pages: 1661-1666
• [Journal Article] Critical role of PA28γ in hepatitis C virus-associated steatogenesis and hepatocarcinogenesis. (2007)
  • Author(s): Moriishi K.
  • Journal Title: PNAS 104 Pages: 1661-1666
  • Peer Reviewed
• [Journal Article] Hepatitis C Virus Nonstructural Protein 5A Modulates TLR-MyD88-Dependent Signaling Pathway in the Macrophage Cell Lines. (2007)
  • Author(s): Abe T.
  • Journal Title: J. Virol. 81 Pages: 8953-8966
  • Peer Reviewed
• [Journal Article] Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins. (2007)
  • Author(s): Tani H.
  • Journal Title: J. Virol. 81 Pages: 8601-8612
  • Peer Reviewed
• [Journal Article] Host factors involved in the replication of hepatitis C virus. (2007)
  • Author(s): Moriishi K.
  • Journal Title: Rev. Med. Virol. 17 Pages: 343-354
  • Peer Reviewed
• [Journal Article] 査読の有無巻発行年最初と最後の頁
  • Author(s): 雑 誌 名
• [Presentation] Proteasome activator PA28g is required for efficient growth of hepatitis C virus (2008)
  • Author(s): Kohji Moriishi and Yoshiharu Matsuura
  • Organizer: 15th International Meeting on HCV and Related Viruses, San Antonio
  • Place of Presentation: October
  • Year and Date: 2008-05-09
• [Presentation] Establishment of an indicator cell line that detects the replication of HCV in sera from hepatitis C patients (2008)
  • Author(s): Yoshinori Tanaka, Yoshio Mori, Hideki Tani, Takayuki Abe, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 15th International Meeting on HCV and Related Viruses, San Antonio
  • Place of Presentation: October
  • Year and Date: 2008-05-09
• [Presentation] Ceramide plays a crucial role on the entry of Japanese encephalitis virus (2008)
  • Author(s): Hideki Tani, Takayuki Izumi, Hiroto Kanbara, Yuuki Kaname, Yoshio Mori, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 15th International Meeting on HCV and Related Viruses, San Antonio
  • Place of Presentation: October
  • Year and Date: 2008-05-09
• [Presentation] HCV infection induces IP-10 production through the TLR signaling pathway (2008)
  • Author(s): Takayuki Abe, Yuuki Kaname, Kohji Moriishi, Tatsuya Kanto, Norio Hayashi, and Yoshiharu Matsuura
  • Organizer: 15th International Meeting on HCV and Related Viruses, San Antonio
  • Place of Presentation: October
  • Year and Date: 2008-05-09
• [Presentation] Human vesicle-associated membrane protein-associated protein subtype C inhibits HCV replication (2008)
  • Author(s): Hiroshi Kukihara, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 15th International Meeting on HCV and Related Viruses, San Antonio
  • Place of Presentation: October
  • Year and Date: 2008-05-09
• [Presentation] C型肝炎ウイルスの増殖と病原性発現に関与する宿主因子 (2008)
  • Author(s): 松浦善治
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会、シンポジウム
  • Place of Presentation: 神戸
• [Presentation] 患者血清中に存在するC型肝炎ウイルスの感染・複製を検出可能な指示細胞の樹立 (2008)
  • Author(s): 田中佳典、森嘉生、谷英樹、阿部隆之、森石恆司、巽正志、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルス感染によるTLR経路を介した炎症性ケモカインIP-10の過剰産生 (2008)
  • Author(s): 阿部隆之、要祐喜、森石恆司、考藤達哉、林紀夫、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルス感染細胞特異的なIFNの誘導によるウイルス排除システムの構築 (2008)
  • Author(s): 阿部隆之、温小玉、田中佳典、寒原裕登、谷英樹、森石恆司、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] ヒトVAP-CはC型肝炎ウイルスの複製を抑制する (2008)
  • Author(s): 久木原博、森石恆司、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] 日本脳炎ウイルスの細胞侵入におけるセラミドの関与 (2008)
  • Author(s): 谷英樹、泉貴之、寒原裕登、要祐喜、森嘉生、森石恆司、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] E型肝炎ウイルス様粒子の形成に重要なアミノ酸の同定と細胞吸着阻害モノクローナル抗体の作製 (2008)
  • Author(s): 森嘉生、山下哲生、嶋亮一、森石恆司、李天成、武田直和、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C 型肝炎ウイルス感染におけるプロテアソーム活性化蛋白質PA28γの役割 (2008)
  • Author(s): 森石恆司、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルスの複製におけるhB-ind1のコシャペロン活性 (2008)
  • Author(s): 田鍬修平、阿部隆之、森嘉生、森石恆司、松浦善治
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] IRF7dominant active 変異体によるHCV感染細胞におけるI型IFNの発現増強効果 (2008)
  • Author(s): Xiaoyu Wen、阿部隆之、森石恆司、松浦善治
  • Organizer: 第14回日本遺伝子治療学会
  • Place of Presentation: 札幌
• [Presentation] C型肝炎ウイルスの複製および病原性発現におけるRip の役割 (2008)
  • Author(s): 松浦善治
  • Organizer: 第31回日本神経科学大会ワークショップ
  • Place of Presentation: 東京
• [Presentation] Human vesicle-associated membrane protein-associated protein subtype C inhibits HCV replication. (2008)
  • Author(s): Hiroshi Kukihara
  • Organizer: 15th International Meeting on HCV and Related Viruses
  • Place of Presentation: San Antonio, USA
• [Presentation] HCV infection induces IP-10 production through the TLR signaling pathway. (2008)
  • Author(s): Takayuki Abe
  • Organizer: 15th International Meeting on HCV and Related Viruses
  • Place of Presentation: San Antonio, USA
• [Presentation] Ceramide plays a crucial role on the entry of Japanese encephalitis virus. (2008)
  • Author(s): Hideki Tani
  • Organizer: 15th International Meeting on HCV and Related Viruses
  • Place of Presentation: San Antonio, USA
• [Presentation] Establishment of an indicator cell line that detects the replication of HCV in sera from hepatitis C patients. (2008)
  • Author(s): Yoshinori Tanaka
  • Organizer: 15th International Meeting on HCV and Related Viruses
  • Place of Presentation: San Antonio, USA
• [Presentation] Proteasome activator PA28g is required for efficient growth of hepatitis C virus. (2008)
  • Author(s): Kohji Moriishi
  • Organizer: 15th International Meeting on HCV and Related Viruses
  • Place of Presentation: San Antonio, USA
• [Presentation] C型肝炎ウイルスの複製および病原性発現におけるRipの役割 (2008)
  • Author(s): 松浦善治
  • Organizer: 第31回日本神経科学大会ワークショップ
  • Place of Presentation: 東京
• [Presentation] IRF7dominant active変異体によるHCV感染細胞におけるI型IFNの発現増強効果 (2008)
  • Author(s): Xiaoyu Wen
  • Organizer: 第14回日本遺伝子治療学会
  • Place of Presentation: 札幌
• [Presentation] 分解能3. 5ÅのE型肝炎ウイルス様粒子のX線結晶構造解析 (2008)
  • Author(s): 山下哲生
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルスの複製におけるhB-indlのコシャペロン活性 (2008)
  • Author(s): 田鍬修平
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルス感染におけるプロテアソーム活性化蛋白質PA28γの役割 (2008)
  • Author(s): 森石恒司
  • Organizer: 第56回日本ウイルス学会総会
  • Place of Presentation: 岡山
• [Presentation] Intramembrane processing by SPP regulates membrane localization of HCV core protein and viral propagation (2007)
  • Author(s): Yoshio Mori, Kiyoko Okamoto, Toru Okamoto, Yasumasa Komoda, Masayasu Okochi, Masatoshi Takeda, Tetsuro Suzuki, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 14th International Meeting on HCV and Related Viruses, Glasgow
  • Place of Presentation: September
  • Year and Date: 2007-09-13
• [Presentation] Replication-competent recombinant vesicular stomatitis virus encoding HCV envelope proteins (2007)
  • Author(s): Hideki Tani, Yasumasa Komoda, Eiko Matsuo, Kensuke Suzuki, Itsuki Hamamoto, Tetsuo Yamashita, Kohji Moriishi, Kazuhito Fujiyama, Tatsuya Kanto, Norio Hayashi, Ania Owsianka, Arvind H. Patel, Michael A. Whitt, and Yoshiharu Matsuura
  • Organizer: 14th International Meeting on HCV and Related Viruses, Glasgow
  • Place of Presentation: September
  • Year and Date: 2007-09-13
• [Presentation] Enhancement of IP-10 expression via TLR signaling pathway in cells expressing HCV proteins (2007)
  • Author(s): Takayuki Abe, Yuuki Kaname, Xiaoyu Wen, Kohji Moriishi, Tatsuya Kanto, Norio Hayashi, and Yoshiharu Matsuura
  • Organizer: 14th International Meeting on HCV and Related Viruses, Glasgow
  • Place of Presentation: September
  • Year and Date: 2007-09-13
• [Presentation] Human butyrate-induced transcript 1 interacts with HCV NS5A and regulates HCV replication (2007)
  • Author(s): Shuhei Taguwa, Toru Okamoto, Takayuki Abe, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 14th International Meeting on HCV and Related Viruses, Glasgow
  • Place of Presentation: September
  • Year and Date: 2007-09-13
• [Presentation] C型肝炎ウイルスコア蛋白質のシグナルペプチドペプチダーゼによるプロセッシングの生物学的意義 (2007)
  • Author(s): 森嘉生、岡本貴世子、岡本徹、菰田泰正、鈴木哲朗、森石恆司、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] 分解能3.5ÅのE型肝炎ウイルス様粒子のX線結晶構造解析 (2007)
  • Author(s): 山下哲生、宮崎直幸、森嘉生、森石恆司、李天成、宮村達男、武田直和、吉村政人、月原冨武、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] C型肝炎ウイルスゲノム複製におけるFKBP8の役割 (2007)
  • Author(s): 岡本徹、森石恆司、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] シュードタイプと組換え水疱性口内炎ウイルスを用いたC型肝炎ウイルスの感染機構の解析 (2007)
  • Author(s): 谷英樹、菰田泰正、鈴木健介、山下哲生、稲田大彦、森石恆司、考藤達哉、林紀夫、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] C型肝炎ウイルスによるTLRシグナル伝達経路を介した炎症性ケモカインIP-10の過剰産生 (2007)
  • Author(s): 阿部隆之、森石恆司、考藤達哉、林紀夫、審良静男、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] C型肝炎ウイルスゲノム複製に関与する宿主蛋白質B-ind1の機能解析 (2007)
  • Author(s): 田鍬修平、岡本徹、阿部隆之、森嘉生、森石恆司、松浦善治
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] HCVコア蛋白質による脂肪酸合成促進と肝細胞癌発症におけるPA28γの役割 (2007)
  • Author(s): 森石恆司、森屋恭爾、村田茂穂、田中啓二、鈴木哲朗、宮村達男、小池和彦、松浦善治
  • Organizer: 第43回日本肝臓学会総会ワークショップ
  • Place of Presentation: 東京
• [Presentation] FKBP8 Plays a Crucial Role in the Replication of Hepatitis C Virus (2007)
  • Author(s): Toru Okamoto, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: HEP DART 2007, Hawaii
• [Presentation] Human butyrate-induced transcript 1 interacts with HCV NS5A and regulates HCV replication. (2007)
  • Author(s): Shuhei Taguwa
  • Organizer: 14th International Meeting on HCV and Related Viruses
  • Place of Presentation: Glasgow, UK
• [Presentation] Enhancement of IP-10 expression via TLR signaling pathway in cells expressing HCV proteins. (2007)
  • Author(s): Takayuki Abe
  • Organizer: 14th International Meeting on HCV and Related Viruses
  • Place of Presentation: Glasgow, UK
• [Presentation] Replication-competent recombinant vesicular stomatitis virus encoding HCV envelope proteins. (2007)
  • Author(s): Hideki Tani
  • Organizer: 14th International Meeting on HCV and Related Viruses
  • Place of Presentation: Glasgow, UK
• [Presentation] Intramembrane processing by SPP regulates membrane localization of HCV core protein and viral propagation. (2007)
  • Author(s): Yoshio Mori
  • Organizer: 14th International Meeting on HCV and Related Viruses
  • Place of Presentation: Glasgow, UK
• [Presentation] FKBP8 Plays a Crucial Role in the Replication of Hepatitis C Virus. (2007)
  • Author(s): Toru Okamoto
  • Organizer: HEP DART 2007
  • Place of Presentation: Hawaii, USA
• [Presentation] C型肝炎ウイルスゲノム複製に関与する宿主蛋白質B-indlの機能解析 (2007)
  • Author(s): 田鍬修平
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Presentation] シュードタイプと組換え水庖性口内炎ウイルスを用いたC型肝炎ウイルスの感染機構の解析 (2007)
  • Author(s): 谷 英樹
  • Organizer: 第55回日本ウイルス学会総会
  • Place of Presentation: 札幌
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_matsuura.php