| Project/Area Number |
19390156
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
HASHIGUCHI Teruto Kagoshima University, 大学院・医歯学総合研究科, 准教授 (70250917)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Ikuro 鹿児島大学, 大学院・医歯学総合研究科, 教授 (20082282)
KAWAHARA Koichi 鹿児島大学, 大学院・医歯学総合研究科, 助教 (10381170)
INUI Akio 鹿児島大学, 大学院・医歯学総合研究科, 教授 (80168418)
NATSUGOE Shoji 鹿児島大学, 大学院・医歯学総合研究科, 教授 (70237577)
内村 友則 鹿児島大学, 医学部・歯学部附属病院, 助教 (20363616)
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| Research Collaborator |
TANAKA Kenji , (株)プロトセラ・膜タンパク質&リガンド解析センター
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2007: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | プロテオーム / ペプチドーム / ペプチド / バイオマーカー / 質量分析 / 癌 / DIC / 検査 / 酵素 / 生体分子 / 蛋白質 / peptidome / proteome / peptide / biomarker / inflammation / cancer / diagnosis / degradome / MOF |
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| Research Abstract |
Human serum contains thousands of proteolytically derived low-molecular-weight peptide fragments (serum peptidome). The concept of utilizing the serum peptidome for cancer diagnosis has been developed. A pathological serum peptidome appears when the homeostatic balance between proteases and protease inhibitors is disrupted. We hypothesize if analyses of the serum peptidome are of diagnostic value as information on which molecules are disrupted, and the pathological course it will take in unknown pathological conditions and disseminated intravascular coagulation (DIC). We analyzed the serum peptidome in 3 stages (early stage, pre-DIC and DIC stages) in one patient with POEMS (polyneuropathy, organomegaly, endocrinopathy, M protein and skin changes) syndrome, an intractable disease with unknown pathology, using a 1-dimensional gel electrophoresis/ matrix-assisted laser desorption/ionization-mass spectrometry (1-DE/MS)-based rapid quantitative approach. A very large number of peptide fragments appeared in the DIC stage, compared to pre-DIC. In addition, we identified fragments of transthyretin (ALGISPFHEHAEVVFTANDSGPR, m/z 2,451.18) and α1-antitrypsin (EDPQGDAAQKTDTSHHDQDHPTFN, m/z 2,691.02) that significantly increased in the DIC stage, compared to those in the pre-DIC stage. Rapid analyses of the serum peptidome may lead to a diagnostic method that can predict on-going protease activated pathological conditions and help to decide on multilateral strategies including nutritional support and drug therapy.
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