Bio-kinetics of Metal Nano Particle via Olfactory Neurons after Inhalation to Mice

• Project/Area Number: 19390162
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kyoto University
• Principal Investigator: UCHIYAMA Iwao Kyoto University, 大学院・工学研究科, 教授 (20151897)
• Co-Investigator(Kenkyū-buntansha): TAKAOKA Masaki 京都大学, 大学院・工学研究科, 准教授 (80252485)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: ディーゼル排気 / ナノ粒子 / 嗅覚神経 / マッピング / 放射光 / 嗅神経 / 透過型電子顕微鏡 / 蛍光X線分析 / 金属元素 / コロイダルゴールド / ディーゼル排気中ナノ粒子 / マイクロビーム

Research Abstract

ディーゼル排気中ナノ粒子曝露を行ったマウスを用い, 嗅覚系ニューロンを介在した大脳辺縁系への曝露経路を形態的に解明することを目的とし, 実験を行った。測定は, 放射光施設SPring-8にて, 嗅上皮, 嗅球における各種金属(Ca, Cu, Fe, Ni, Zn)の蛍光X線マッピングをおこなった。その結果, Fe, Ni, Znの値が, 神経の存在する組織層で高い値を示した。粒子の侵入がある可能性が高いことが確認された。

Research Products

• [Journal Article] Tracking the Pathway of Diesel Exhaust Particles (DEP) from the Olfactory Bulb to the Central Nervous System by X-ray Fluorescence Analysis (2008)
  • Author(s): Yasuto Matsui
  • Journal Title: Spectrochimica Acta PartB 63 Pages: 433-440
  • Peer Reviewed
• [Journal Article] Tracking the Pathway of Diesel ExhaustParticles (DEP) from the Olfactory Bulb tothe Central Nervous System by X-rayFluorescence Analysis (2007)
  • Author(s): Y. Matsui, N. Sakai, A. Tsuda, Y. Terada, M. Takaoka, H. Fujimaki, I. Uchiyama
  • Journal Title: Spectrochimica Acta PartB 29(3) Pages: 428-433
  • Peer Reviewed
• [Journal Article] 二酸化チタン超微小粒子曝露によるマウス体内分布に関する研究 (2007)
  • Author(s): 坂井 伸光
  • Journal Title: 産業医科大学雑誌 29(3) Pages: 428-433
  • Peer Reviewed
• [Journal Article] Risk assessment of Ti02 photocatalyst by individua1 micrometer-size particle analysis with on-site combination of SEK-EDX and SH-XANES microscope (2007)
  • Author(s): Jun Kawai
  • Journal Title: Spectrochimica Acta PartB 62 Pages: 677-681
  • Peer Reviewed
• [Presentation] Tracking a Pathway Diesel Exhaust Particle from Nose to Brain (2009)
  • Author(s): Yasuto Matsui
  • Organizer: American Thoracic Society
  • Place of Presentation: San Diego/USA
  • Year and Date: 2009-05-18
• [Presentation] Determination of formaldehyde hemoglobin adducts in mice as a biological exposure index for multiple chemical sensitivity (2008)
  • Author(s): Keisuke Kodera
  • Organizer: American Thoracic Society
  • Place of Presentation: Toronto/CANADA
  • Year and Date: 2008-05-16
• [Presentation] Uptake of Nanoparticles by the Sensory Nerves in the Pulmonary Alveoli of Mice (2008)
  • Author(s): Yasuto Matsui
  • Organizer: American Thoracic Society
  • Place of Presentation: Toronto/CANADA
  • Year and Date: 2008-05-16
• [Presentation] Imaging of Olfactory Nerves after Intranasal Administration of Ultrafine Colloidal Gold in Mouse (2008)
  • Author(s): Nobumitsu Sakai
  • Organizer: American Thoracic Society
  • Place of Presentation: Toronto/CANADA
  • Year and Date: 2008-05-16
• [Presentation] Synchrotron imaging of Diesel Exhaust Particles in the Olfactory Nerve (2008)
  • Author(s): Yasuto Matsui
  • Organizer: American Thoracic Society
  • Place of Presentation: Toronto/CANADA
  • Year and Date: 2008-05-16
• [Presentation] Determination of formaldehyde hemoglobin adducts in mice as a biological exposure index for multiple chemical sensitivity (2008)
  • Author(s): Keisuke Kodera
  • Organizer: American Thoracic Society
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2008-05-16
• [Presentation] Uptake of Nanoparticles by the Sensory Nerves in the Pulmonary Alveoli of Mice (2008)
  • Author(s): Yasuto Matsui
  • Organizer: American Thoracic Society
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2008-05-16
• [Presentation] Imaging of Olfactory Nerves after Intranasal Administration of Ultrafine Colloidal Gold in Mouse (2008)
  • Author(s): Nobumitsu Sakai
  • Organizer: American Thoracic Society
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2008-05-16
• [Presentation] Synchrotron imaging of Diesel Exhaust Particles in the Olfactory Nerve (2008)
  • Author(s): Yasuto Matsui
  • Organizer: American Thoracic Society
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2008-05-16
• [Presentation] Pathway for ultrafine diesel exhaust particles from the nose to the brain (2007)
  • Author(s): Yasuto Matsui
  • Organizer: Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] Visualization of Nerves and Uptake of Particles to the Nerves in the Pulmonary Alveoli of Rat (2007)
  • Author(s): Keisuke Kodera
  • Organizer: Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] The imaging of olfactory nerves in the mouse after intranasal administration of colloidal gold by TEM-EELS (2007)
  • Author(s): Nobumitsu Sakai
  • Organizer: Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] To track a DEP path from the nose to brain by X-ray fluorescence analysis (2007)
  • Author(s): Yasuto Matsui
  • Organizer: ICXOM
  • Place of Presentation: Kyoto, Japan
• [Presentation] The X-ray absorption fine structure (XAFS) analys is of nann metal fumes trapped by Differential Mobility (2007)
  • Author(s): Nobumitsu Sakai
  • Organizer: Neuroscience
  • Place of Presentation: Kyoto, Japan
• [Presentation] Olfactus-Mediated Transport of Diesel Exhaust Particles from nose to brain in Mice (2007)
  • Author(s): Yasuto Matsui
  • Organizer: ATS 2007
  • Place of Presentation: Kyoto, Japan
• [Presentation] 嗅神経を介在したディーゼル排気中ナノ粒子の曝露経路の検索 (2007)
  • Author(s): 松井 康人
  • Organizer: 大気環境学会
  • Place of Presentation: 岡山,日本
• [Presentation] 肺における神経を介したナノ粒子曝露経路の検索 (2007)
  • Author(s): 小寺 恵介
  • Organizer: 大気環境学会
  • Place of Presentation: 岡山,日本
• [Presentation] 蛍光粒子点鼻投与によるナノ粒子の嗅覚系ニューロンを介した嗅覚中枢への曝露経路の検索 (2007)
  • Author(s): 坂井 伸光
  • Organizer: 大気環境学会
  • Place of Presentation: 岡山,日本
• [Book] ナノ粒子の生理学的活性・呼吸器 (2009)
  • Author(s): 内山巌雄, 松井康人ほか
  • Total Pages: 296
  • Publisher: 中外医学社
• [Book] ナノ粒子の生理学的活性・呼吸器2009 (2009)
  • Author(s): 内山巌雄, 松井康人, ほか
  • Total Pages: 296
  • Publisher: 中外医学社