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Evaluation of genetic instability and radiation sensitivity in human hematolymphoid cells among the atomic-bomb survivor cohort

Research Project

Project/Area Number 19390170
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionRadiation Effects Research Foundation

Principal Investigator

KUSUNOKI Yoichiro  Radiation Effects Research Foundation, 放射線生物学/分子疫学部, 部長代理 (60333548)

Co-Investigator(Kenkyū-buntansha) HAMASAKI Kanya  財団法人放射線影響研究所, 遺伝学部, 研究員 (80443597)
IMAI Kazue  財団法人放射線影響研究所, 放射線生物学/分子疫学部, 研究員 (80260230)
YOSHIDA Kengo  財団法人放射線影響研究所, 放射線生物学/分子疫学部, 研究員 (70443596)
京泉 誠之  安田女子大学, 家政学部, 教授 (50333547)
Co-Investigator(Renkei-kenkyūsha) KYOIZUMI Seishi  安田女子大学, 家政学部, 教授 (50333547)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsゲノム / 放射線 / 突然変異 / 血液細胞
Research Abstract

The human genome (the complete set of genetic information in people's DNA) somewhat differs among individuals, and such variation is in part responsible for how individuals differ in their susceptibility to radiation-related DNA damage and diseases. We detected radiation-related DNA damage in blood cells of A-bomb survivors and investigated associations between frequencies of DNA-damaged cells and genotypes of DNA repair genes. Our results suggest that the difference in the genotype of P53BP1 may be involved in the individual's difference in the frequency of GPA mutant blood cells.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (58 results)

All 2010 2009 2008 2007 Other

All Journal Article (21 results) (of which Peer Reviewed: 20 results) Presentation (34 results) Remarks (3 results)

  • [Journal Article] T-cell immunosenescence and inflammatory response in atomic-bomb survivors.2010

    • Author(s)
      Kusunoki Y, Yamaoka M, Kubo Y, Hayashi T, Kasagai F, Douple EB, Nakachi K
    • Journal Title

      Radiat Res (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased DNA damage in hematopoietic cells of mice with graft-versus-host disease.2010

    • Author(s)
      Kusunoki Y, Hamasaki K, Koyama K, Imai K, Hayashi T, Martin PJ, Nakachi K
    • Journal Title

      Mutat Res. (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Memory CD4 T-cell subsets discriminated by CD43 expression level in A-bomb survivors.2010

    • Author(s)
      Kyoizumi S, Yamaoka M, Kubo Y, Hamasaki K, Hayashi T, Nakachi K, Kasagi F, Kusunoki Y
    • Journal Title

      Int J Radiat Biol 86

      Pages: 56-62

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased DNA damage in hematopoietic cells of mice with graft-versus-host disease2010

    • Author(s)
      Kusunoki Y
    • Journal Title

      Mutat.Res. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] T-cell immunosenescence and inflammatory response in atomic-bomb survivors2010

    • Author(s)
      Kusunoki Y
    • Journal Title

      Radiat. Res. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Memory CD4 T-cell subsets discriminated by CD43 expression level in A-bomb survivors2010

    • Author(s)
      Kyoizumi S
    • Journal Title

      Int.J.Radiat. Biol. 86

      Pages: 56-62

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lung cancer susceptibility among atomic-bomb survivors in relation to CA repeat number polymorphism of epidermal growth factor receptor gene and radiation dose.2009

    • Author(s)
      Yoshida K, Nakachi K, Imai K, Cologne JB, Niwa Y, Kusunoki Y, Hayashi T
    • Journal Title

      Carcinogenesis 30

      Pages: 2037-2041

    • Related Report
      2009 Final Research Report
  • [Journal Article] Clonally expanded T lymphocytes from atomic bomb survivors in vitro show no evidence of cytogenetic instability.2009

    • Author(s)
      Hamasaki K, Kusunoki Y, Nakashima E, Takahashi N, Nakachi K, Nakamura N, Kodama Y
    • Journal Title

      Radiat Res. 172

      Pages: 234-243

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells.2009

    • Author(s)
      Yoshida K, Kubo Y, Kusunoki Y, Morishita Y, Nagamura H, Hayashi I, Kyoizumi S, Seyama T, Nakachi K, Hayashi T
    • Journal Title

      Cell Immunol 255

      Pages: 61-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clonally expanded T lymphocytes from atomic bomb survivors in vitro show noevidence of cytogenetic instability2009

    • Author(s)
      Hamasaki K
    • Journal Title

      Radiat. Res. 172

      Pages: 234-243

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells2009

    • Author(s)
      Yoshida K
    • Journal Title

      Cell Immunol. 255

      Pages: 61-68

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lung cancer susceptibility among atomic-bomb survivors in relation to CA repeat number polymorphism of epidermal growth factor receptor gene and radiation dose2009

    • Author(s)
      Yoshida K
    • Journal Title

      Carcinogenesis 30

      Pages: 2037-2041

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells2009

    • Author(s)
      Yoshida K
    • Journal Title

      Cellular Immunology 255

      Pages: 61-68

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytolethal distending toxin induces caspase-dependent and-independent cell death in MOLT-4 cells.2008

    • Author(s)
      Ohara M, Hayashi T, Kusunoki Y, Nakachi K, Fujiwara T, Komatsuzawa H, Sugai M
    • Journal Title

      Infect Immun 76

      Pages: 4783-4791

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sixty years of follow-up of Hiroshima and Nagasaki survivors: Current progress in molecular epidemiology studies.2008

    • Author(s)
      Nakachi K, Hayashi T, Hamatani K, Eguchi H, Kusunoki Y
    • Journal Title

      Mutat Res. 659

      Pages: 109-117

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-lasting alterations of the immune system by ionizing radiation exposure: Implications for disease development among atomic bomb survivors.2008

    • Author(s)
      Kusunoki Y, Hayashi T
    • Journal Title

      Int J Radiat Biol 84

      Pages: 1-14

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hiroshima and Nagasaki 60 years : Follow-up of survivors -Current progress in molecular epidemiology studies-2008

    • Author(s)
      Nakachi K
    • Journal Title

      Mutation Research 659

      Pages: 109-117

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytolethal distending toxin induces caspase-dependent and-independent cell death in MOLT-4 cells2008

    • Author(s)
      Ohara M
    • Journal Title

      Infection and Immunity 76

      Pages: 4783-4791

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-lasting alterations of the immune system by ionizing radiation exposure : Implications for disease development among atomic bomb survivors2008

    • Author(s)
      Kusunoki Y
    • Journal Title

      Int J Radiat Biol 84

      Pages: 1-14

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation sensitivity and genomic instability in the hematopoietic system: Frequencies of micronucleated reticulocytes in whole-body X-irradiated BALB/c and C57BL/6 mice.2007

    • Author(s)
      Hamasaki K, Imai K, Hayashi T, Nakachi K, Kusunoki Y
    • Journal Title

      Cancer Sci. 98

      Pages: 1840-1844

    • NAID

      10019996027

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Radiation sensitivity and genomic instability in the hematopoietic system : Frequencies of micronucleated reticulocytes in whole-body X-irradiated BALB/c and C57BL/6 mice2007

    • Author(s)
      Hamasaki K
    • Journal Title

      Cancer Sci 98

      Pages: 1840-1844

    • NAID

      10019996027

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 原爆被爆者の高齢化と免疫学的変化2009

    • Author(s)
      楠洋一郎
    • Organizer
      2009年放射線影響協会講演会「放射線被ばくとがんの関係性について」
    • Place of Presentation
      東京
    • Year and Date
      2009-11-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス造血系における炎症とゲノム不安定性.2009

    • Author(s)
      濱崎幹也
    • Organizer
      第52回日本放射線影響学会
    • Place of Presentation
      広島
    • Related Report
      2009 Final Research Report
  • [Presentation] Impact of ATM, ATR, NBS1 genetic polymorphisms on radiation-associated cancer risks in atomic-bomb survivors.2009

    • Author(s)
      Yoshida K
    • Organizer
      Keystone Symposia on Molecular and Cellular Biology: Telomere Biology and DNA Repair
    • Place of Presentation
      Ashmore, Australia
    • Related Report
      2009 Final Research Report
  • [Presentation] 網状赤血球小核頻度解析による原爆被爆者造血系の放射線誘発遺伝的不安定性の評価.2009

    • Author(s)
      楠洋一郎
    • Organizer
      第19回日本サイトメトリー学会学術集会
    • Place of Presentation
      松江
    • Related Report
      2009 Final Research Report
  • [Presentation] Increases in the percentages of CD43-low memory and CD25+/CD127- regulatory T cells in the CD4 T-cell populations among A-bomb survivors.2009

    • Author(s)
      Kusunoki Y
    • Organizer
      The 5th Kyoto T Cell Conference (KTCC) 2009 International Workshop on T Lymphocytes
    • Place of Presentation
      Kyoto
    • Related Report
      2009 Final Research Report
  • [Presentation] Immunological alterations in aging A-bomb survivors.2009

    • Author(s)
      Kusunoki Y
    • Organizer
      Late Health Effects of Ionizing Radiation: Bridging the Experimental and Epidemiologic Divide
    • Place of Presentation
      Georgetown University, Washington DC, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] 原爆被爆者のCD4T細胞集団におけるCD43-lowメモリーT細胞およびCD25+/CD127-制御性T細胞の比率の増加2009

    • Author(s)
      楠洋一郎
    • Organizer
      第5回 京都T細胞学術会議2009年Tリンパ球に関する国際ワークショップ
    • Place of Presentation
      京都
    • Related Report
      2009 Annual Research Report
  • [Presentation] 網状赤血球小核頻度解析による原爆被爆者造血系の放射線誘発遺伝的不安定性の評価2009

    • Author(s)
      楠洋一郎
    • Organizer
      第19回 日本サイトメトリー学会学術集会
    • Place of Presentation
      松江
    • Related Report
      2009 Annual Research Report
  • [Presentation] Impact of ATM, ATR, and NBS1 genetic polymorphisms on radiation-associated cancer risks in atomic-bomb survivors2009

    • Author(s)
      Yoshida K
    • Organizer
      Keystone Symposia on Molecular and Cellular Biology : Telomere Biology and DNA Repair
    • Place of Presentation
      Ashmore, Australia
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス造血系における炎症とゲノム不安定性2009

    • Author(s)
      濱崎幹也
    • Organizer
      第52回日本放射線影響学会
    • Place of Presentation
      広島
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト末梢血CD8TおよびNK細胞集団のNKG2D細胞表面発現に関係した遺伝子型2009

    • Author(s)
      楠洋一郎
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] C型肝炎ウイルス感染におけるNKG2D伝子多型の影響2009

    • Author(s)
      吉田健吾
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic instability of the hematopoietic system in murine inflammation models2009

    • Author(s)
      Kusunoki Y
    • Organizer
      The 10th International Symposium on Dendritic Cells
    • Place of Presentation
      Kobe, Hyogo
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of genetic instability and somatic mutation assays in radiation-exposed individuals.2008

    • Author(s)
      Kusunoki Y
    • Organizer
      International Symposium on Genotoxicity Assessment, The 37th Annual Meeting of the Japanese Environmental Mutagen Society
    • Place of Presentation
      Okinawa
    • Related Report
      2009 Final Research Report
  • [Presentation] Genetic instability of the hematopoietic system in murine inflammation models.2008

    • Author(s)
      Kusunoki Y
    • Organizer
      The 10th International Symposium on Dendritic Cells
    • Place of Presentation
      Kobe
    • Related Report
      2009 Final Research Report
  • [Presentation] Genomic instability persisting in vivo for prolonged periods after irradiation: Elevated micronucleated reticulocyte frequencies in mice one year after whole-body irradiation.2008

    • Author(s)
      Hamasaki K
    • Organizer
      The 54th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      Boston, Massachusetts, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] T-cell aging radiation-exposed individuals. U.S. -Japan Cooperative Medical Science Program2008

    • Author(s)
      Kusunoki Y
    • Organizer
      Immunology Board: Immunosenescence Workshop
    • Place of Presentation
      San Francisco, California, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] Mouse strain difference in sensitivity to radiation-induced genomic instability persisting in vivo for prolonged periods after irradiation.2008

    • Author(s)
      Kusunoki Y
    • Organizer
      The International Ataxia-Telangiectasia Workshop 2008
    • Place of Presentation
      Otsu
    • Related Report
      2009 Final Research Report
  • [Presentation] Mouse strain difference in sensitivity to radiation-induced genomic instability persisting in vivo for prolonged periods after irradiation2008

    • Author(s)
      Kusunoki Y
    • Organizer
      The International Ataxia-Telangiectasia Workshop 2008
    • Place of Presentation
      Ohtsu, Shiga
    • Related Report
      2008 Annual Research Report
  • [Presentation] T-cell aging in radiation-exposed individuals2008

    • Author(s)
      Kusunoki Y
    • Organizer
      U. S.-Japan Cooperative Medical Science Program, Immunology Board : Immunosenescence Workshop
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genomic instability persisting in vivo for prolonged periods after irradiation : Elevated micronucleated reticulocyte frequencies in mice one year after whole-body irradiation2008

    • Author(s)
      Hamasaki K
    • Organizer
      The 54th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      Boston, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Radiosensitivity of A-bomb survivors pregnant at the time of bombings in Hiroshima and Nagasaki2008

    • Author(s)
      Miles EF
    • Organizer
      The 54th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      Boston, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of genetic instability and somatic mutation assays in radiation-exposed individuals2008

    • Author(s)
      Kusunoki Y
    • Organizer
      International Symposium on Genotoxicity Assessment
    • Place of Presentation
      Okinawa
    • Related Report
      2008 Annual Research Report
  • [Presentation] 原爆被爆者末梢血リンパ球の in vitro 長期培養クローンにおける染色体不安定性について2007

    • Author(s)
      濱崎幹也
    • Organizer
      第48回 原子爆弾後障害研究会
    • Place of Presentation
      広島
    • Year and Date
      2007-06-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] X線を全身照射したBALB/cおよびC57BL/6マウスにおける小核網状赤血球のフローサイトメトリーによる解析:遺伝的不安定性が照射後長期にわたって生体内で持続する証拠.2007

    • Author(s)
      濱崎幹也
    • Organizer
      第50回日本放射線影響学会
    • Place of Presentation
      千葉
    • Related Report
      2009 Final Research Report
  • [Presentation] 原爆被爆者集団における末梢血ナイーブCD4T細胞比率の加齢あるいは被ばく線量依存性の低下にCD45遺伝子多型との関連性は見られない.2007

    • Author(s)
      楠洋一郎
    • Organizer
      第17回日本サイトメトリー学会
    • Place of Presentation
      浦安
    • Related Report
      2009 Final Research Report
  • [Presentation] A genome approach to inter-individual variations in cancer susceptibility among atomic-bomb survivors2007

    • Author(s)
      Yoshida K
    • Organizer
      12th Congress of the International Association of Biomedical Gerontology(IABG)
    • Place of Presentation
      Spetses island, Greece
    • Related Report
      2007 Annual Research Report
  • [Presentation] Hiroshima 60 years : Monitoring of survivors-Current progress in molecular epidemiology studies2007

    • Author(s)
      Nakachi K
    • Organizer
      5th International Conference on Environmental Mutagens in Human Populations
    • Place of Presentation
      Antalya, Turkey
    • Related Report
      2007 Annual Research Report
  • [Presentation] 原爆被爆者集団における末梢血ナイーブCD4T細胞比率の加齢あるいは被ばく線量依存性の低下にCD45遺伝子多型との関連性は見られない2007

    • Author(s)
      楠 洋一郎
    • Organizer
      第17回 日本サイトメトリー学会
    • Place of Presentation
      浦安
    • Related Report
      2007 Annual Research Report
  • [Presentation] Cytogenetic instability in peripheral blood T lymphocytes cultured in vitro from A-bomb survivors2007

    • Author(s)
      Hamasaki K
    • Organizer
      13th International Congress of Radiation Research
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Chromosome tests in clonally proliferated T lymphocytes in vitro from A-bomb survivors do not suggest presence of genetic instability2007

    • Author(s)
      Hamasaki K
    • Organizer
      8th International Symposium on Chromosome Aberrations
    • Place of Presentation
      Awaji, Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] X線を全身照射したBALB/cおよびC57BL/6マウスにおける小核網状赤血球のフローサイトメトリーによる解析 : 遺伝的不安定性が照射後長期にわたって生体内で持続する証拠2007

    • Author(s)
      濱崎幹也
    • Organizer
      第50回 日本放射線影響学会
    • Place of Presentation
      千葉
    • Related Report
      2007 Annual Research Report
  • [Presentation] 慢性C型肝炎ウイルス感染の肝病態の進行にともなうThl優位の免疫応答2007

    • Author(s)
      楠 洋一郎
    • Organizer
      第37回 日本免疫学会総会・学術集会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] 炎症関連サイトカイン遺伝子多型と原子放射線被曝が胃がんリスクに及ぼす影響2007

    • Author(s)
      林 奉権
    • Organizer
      第37回 日本免疫学会総会・学術集会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.rerf.jp/library/archives/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.rerf.jp/library/archives/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.rerf.jp/library/rr/rr0708.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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