Pharmacological study to cure mibyo, disease-oriented state

• Project/Area Number: 19390193
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Sojo University
• Principal Investigator: MIYATA Takeshi Sojo University, 薬学部, 教授 (90040310)
• Co-Investigator(Kenkyū-buntansha): ISIHAMA Yoichiro 熊本大学, 大学院・生命科学研究部, 准教授 (10240920) ; YOKOMIZO Kazumi 崇城大学, 薬学部, 准教授 (80240919) ; HISATSUNE Akinori 熊本大学, 大学院・生命科学研究部, 助教 (50347001)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 東洋医学 / 未病 / 抗MUC1抗体 / 抗腫瘍 / グリチルリチン / インターフェロン-γ / 診断マーカー / MUC1

Research Abstract

Curing disease-oriented state, very early stage of diseases before they appear, is important to preserve health and prevent illness. In this study, we found that anti-MUC1 antibody, a new anti-cancer biomarker, inhibits cancer cell growth. We also found that glycyrrhizin has glucocorticoid-like anti-inflammatory effect but not through glucocorticoid receptor. These finding may provide a new insight to care disease-oriented state.

Research Products

• [Journal Article] Glycyrrhizin attenuates mucus production by inhibition of MUC5AC mRNA expression in vivo and in vitro. (2010)
  • Author(s): Nishimoto Y, Hisastsune A, Katsuki H, Miyata T, Yokomizo K, Isohama Y
  • Journal Title: J Pharmacol Sci (in press)
  • NAID: 10027745175
  • Peer Reviewed
• [Journal Article] Glycyrrhizin Attenuates Mucus Production by Inhibition of MUC5AC mRNA Expression In Vivo and In Vitro. (2010)
  • Author(s): Nishimoto Y, ほか
  • Journal Title: J Pharmacol Sci 112(In press)
  • Peer Reviewed
• [Journal Article] Internalization of MUCI by anti-MUCI antibody from cell membrane through the macropinocytotic pathway. (2009)
  • Author(s): Hisastsune A, Kawasaki M, Nakayama H, Mikami Y, Miyata T, Isohama Y, Katsuki H, Kim KC.
  • Journal Title: Biochem Biophys Res Commun 388 Pages: 677-682
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor-αdecreases aquaporin-3 expression in DJM-1 keratinocytes. (2009)
  • Author(s): Horie I, Maeda M, Yokoyama S, Hisatsune A, Katsuki H, Miyata T, Isohama Y.
  • Journal Title: Biochem Biophys Res Commun. 387 Pages: 564-568
  • Peer Reviewed
• [Journal Article] Bacterial neuraminidase increases IL-8 production in lung epithelial cells viaNF-kappaB-dependent pathway. (2009)
  • Author(s): Kuroiwa A, ほか
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 754-759
  • Peer Reviewed
• [Journal Article] Hypoxia enhances MUC1 expression in a lung adenocarcinoma cell line. (2009)
  • Author(s): Mikami Y, ほか
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 1060-1065
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor-alpha decreases aquaporin-3 expression in DJM-1 keratinocytes. (2009)
  • Author(s): Horie I, ほか
  • Journal Title: Biochem Biophys Res Commun 388 Pages: 677-682
  • Peer Reviewed
• [Journal Article] Hypoxia enhances MUC1 expression in a lung adenocarcinoma cell line. (2009)
  • Author(s): Mikami Y (第一著者)
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 1060-1065
  • Peer Reviewed
• [Journal Article] Bacterial neuraminidase increases IL-8 production in lung epithelial cells via NF-kappaB-dependent pathway. (2009)
  • Author(s): Kuroiwa A (第一著者)
  • Journal Title: Biochem Biophys Res Commun. 379 Pages: 754-759
  • Peer Reviewed
• [Journal Article] Glycyrrhizin inhibits interleukin-8 production and nuclear factor-kappaB activity in lung epithelial cells, but not through glucocorticoid receptors. (2008)
  • Author(s): Takei H, Baba Y, Hisatsune A, Katsuki H, Miyata T, Yokomizo K, Isohama Y.
  • Journal Title: J Pharmacol Sci 106 Pages: 460-468
  • Peer Reviewed
• [Journal Article] Estrogenic activities of Fatty acids and a sterol isolated from royal jelly. (2008)
  • Author(s): Suzuki K (第一著者)
  • Journal Title: Evid Based Complement Alternat Med. 5 Pages: 295-302
  • Peer Reviewed
• [Journal Article] Glycyrrhizin inhibits interleukin-8 production and nuclear factor-kappaB activity in lung epithelial cells, but not through (2008)
  • Author(s): Takei H (第一著者)
  • Journal Title: J Pharmacol Sci 106 Pages: 460-468
  • Peer Reviewed
• [Journal Article] The signaling pathway involved in neutrophil elastase stimulated MUC1 transcription (2008)
  • Author(s): Kuwahara I(第一著者名)
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 106
  • Peer Reviewed
• [Journal Article] TNF-α induces MUCI gene transcription in lung epithelial cells: its signaling pathway and biological implication. (2007)
  • Author(s): Koga T, Kuwahara I, Lillehoj EP, Lu W, Miyata T, Isohama Y, Kim KC.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 293 Pages: 693-701
  • Peer Reviewed
• [Journal Article] The signaling pathway involved in neutrophil elastase stimulated MUCI transcription. (2007)
  • Author(s): Kuwahara I, Lillehoj EP, Koga T, Isohama Y, Miyata T, Kim KC.
  • Journal Title: Am J Respir Cell Mol Biol 37 Pages: 691-698
  • Peer Reviewed
• [Journal Article] Nitric oxide decreases cell surface expression of aquaporin-5 and membrane water permeability in lung epithelial cells (2007)
  • Author(s): Nagai K(第一著者名)
  • Journal Title: Biochem Biophys Res Commun 354 Pages: 579-584
  • Peer Reviewed
• [Journal Article] TNF-alpha induces MUC1 gene transcription in lung epithelial cells: its signaling pathway and biological implication (2007)
  • Author(s): Koga T(第一著者名)
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 293
  • Peer Reviewed
• [Journal Article] Glycyrrhizin inhibits interleukin-8 production and NF-κ B activity in lung epithelial cells,but not through glucocorticoid receptors (2007)
  • Author(s): Takei H(第一著者名)
  • Journal Title: J Phamrmacol Sci 293 Pages: 460-468
  • Peer Reviewed
• [Presentation] ケラチノサイトの水チャネルaquaporin-3の発現を増加させる生薬の探索研究 (2009)
  • Author(s): 堀江一郎, 久恒昭哲, 香月博志, 宮田健, 礒濱洋一郎
  • Organizer: 第26回和漢医薬学会大会
  • Place of Presentation: 千葉市
  • Year and Date: 2009-08-30
• [Presentation] ケラチノサイトの水チャネルaquaporin-3の発現を増加させる生薬の探索研究 (2009)
  • Author(s): 堀江一郎, ほか
  • Organizer: 第26回和漢医薬学会大会
  • Place of Presentation: 千葉
  • Year and Date: 2009-08-29
• [Presentation] シアリダーゼの肺上皮細胞における炎症性サトカイン発現に対する影響 (2009)
  • Author(s): 久恒昭哲, ほか
  • Organizer: 第29回気道分泌研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-25
• [Presentation] 低酸素条件下におけるMUClのHIF非依存的な発現調節 (2009)
  • Author(s): 見上裕士(代表者)
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-03-18
• [Presentation] 肺上皮細胞における細菌由来ノイラミニダーゼのサイトカイン発現作用 (2009)
  • Author(s): 黒岩覚(代表者)
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-03-16
• [Presentation] In vivoおよびin vitro実験系における気道粘液産生亢進に対するglycyrrhizinの抑制作用 (2008)
  • Author(s): 西本裕樹(代表者)
  • Organizer: 第61回日本薬理学会西南部会
  • Place of Presentation: 米子市
  • Year and Date: 2008-11-28
• [Presentation] 漢方薬の呼吸器疾患治療効果から見えてきたグリチルリチンの気道粘液産生制御作用 (2008)
  • Author(s): 礒濱洋一郎(代表者)
  • Organizer: 第17回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-14
• [Presentation] 補気剤のもつ抗炎症作用の分子機序-補中益気湯および構成成分によるNF-κB阻害作用 (2008)
  • Author(s): 礒濱洋一郎(代表者)
  • Organizer: 第25回和漢医薬学会大会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-08-31
• [Presentation] 麦門冬による鎮咳作用の薬理学的特性 (2008)
  • Author(s): 礒濱洋一郎(代表者)
  • Organizer: 第48回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-06-17
• [Presentation] グリチルリチンの気道粘液産生抑制作用の薬理学的特性 (2008)
  • Author(s): 礒濱洋一郎, 西本裕樹, 久恒昭哲, 香月博志, 宮田健
  • Organizer: 第27回気道分泌研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-04-12
• [Presentation] 膜結合型ムチンMUC1は特異的抗体の結合によって細胞内局在が変化する (2007)
  • Author(s): 川崎 美鶴(代表者)
  • Organizer: 第60回日本薬学会西南部会
  • Place of Presentation: 宮崎市
  • Year and Date: 2007-11-22
• [Presentation] アガリクス培養物の生理活性について (2007)
  • Author(s): 横溝 和美(代表者)
  • Organizer: 第14回日本未病システム学会
  • Place of Presentation: 金沢市
  • Year and Date: 2007-11-02
• [Presentation] 美露仙寿の食欲・体力増進作用の薬理学的解析 (2007)
  • Author(s): 周 建融(代表者)
  • Organizer: 第14回日本未病システム学会
  • Place of Presentation: 金沢市
  • Year and Date: 2007-11-02
• [Presentation] 甘草の主成分グリチルリチンのユニークな抗炎症作用 (2007)
  • Author(s): 礒濱 洋一郎(代表者)
  • Organizer: 第2回食品薬学シンポジウム
  • Place of Presentation: 静岡市
  • Year and Date: 2007-10-18
• [Presentation] 補中益気湯の抗炎症作用の薬理学的特性 (2007)
  • Author(s): 武井啓典, 小黒夏子, 久恒昭哲, 香月博志, 宮田健, 礒濱洋一郎
  • Organizer: 第24回和漢医薬学会大会
  • Place of Presentation: 富山市
  • Year and Date: 2007-09-08
• [Presentation] 補中益気湯の抗気道炎症作用の薬理学的特性 (2007)
  • Author(s): 武井 啓典(代表者)
  • Organizer: 第24回和漢医薬学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-09-08
• [Presentation] 気道上皮細胞に対する麦門冬湯の薬理学的特性 (2007)
  • Author(s): 礒濱 洋一郎(代表者)
  • Organizer: 第26回漢方・免疫・アレルギー研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-23