Development of a novel method for epigenome analysis and application to cancer diagnosis and order made therapy

• Project/Area Number: 19390201
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: TOYOTA Minoru Sapporo Medical University, 医学部, 教授 (70270676)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Hiromu 札幌医科大学, 医学部, 助教 (20381254)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2008: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 消化器癌 / DNAメチル化 / エピジェネティクス / 遺伝子診断 / クロマチン / エピゲノム / ヒストンメチル化 / RNA干渉 / オーダーメード医療 / ヒストン / 個別化医療

Research Abstract

消化器癌においてジェネティックな異常に加え、DNAメチル化やヒストン修飾異常などのエピジェネティックな異常が関与する。ゲノム上の多数の遺伝子がエピジェネティックな異常を有すると考えられ、その全貌を明らかにすることは、消化器癌の診断および治療法開発の上で重要である。本研究では、ゲノム網羅的メチル化解析法と多数の検体を定量的に解析する方法の開発を目指す。Methylated CpG island amplification (MCA)法により、メチル化しているCpG islandを効率よく増幅し、大腸癌細胞株から抽出したDNAから作成したMCAアンプリコンをCy5で、正常大腸組織より抽出したDNAから作成したMCAアンプリコンをCy3でラベルし、Agilent社のwhole genome promoter arrayにハイブリダイズすることにより得られたシグナルを解析し、高メチル化している遺伝子を同定した。また、得られた結果を、ゲノムブラウザBLAT上に表示し、クロマチン免疫沈降法(ChIP on ChIP)のデータと比較することにより、DNAメチル化、ヒストンH3リジン4、リジン27のメチル化との統合解析を可能にした。DNMTあるいはDicerを遺伝学的にノックアウトした大腸癌細胞を用いて、MCA microarrayを行い、DNAメチル化およびヒストン修飾のプロファイルを作成した。本研究により開発された新しいエピゲノム法により消化器癌発生の分子機構の解明や、診断や治療の標的遺伝子の同定に有用と考えられる。

Research Products

• [Journal Article] Sensitive and specific detection of early gastric cancer using DNA methylation analysis of gastric washing (2009)
  • Author(s): Watanabe, Y, Kim, HS, Castoro, RJ, Chyng, W, Estecio, MR, Kondo, K, Guo, Y, Ahmed, SS, Toyota, M, Itoh, F, Suk, KT, Cho, MY, Shen, L, Jelinek, J and Issa, JP.
  • Journal Title: Gastroenterol, Published on line
  • Peer Reviewed
• [Journal Article] CHFR, a potent tumor suppressor, downregulates interleukin-8 via inhibition of NF-・B (2009)
  • Author(s): Kashima L, Toyota M, Mita H, Suzuki H, Idogawa M, Ogi K, Sasaki Y, , Tokino T.
  • Journal Title: Oncogene (in press)
  • Peer Reviewed
• [Journal Article] Genomic Screening for Genes Silenced by DNA Methylation Revealed an Association between RASD1 Inactivation and Dexamethasone Resistance in Multiple Myeloma (2009)
  • Author(s): Nojima M, Maruyama R, Yasui H, Suzuki H, Maruyama Y, Tarasawa I, Sasaki Y, Asaoku H, Sakai H, Hayashi T, Mori M, Imai K, Tokino T, Ishida T, Toyota M, Shinomura Y.
  • Journal Title: Clin Cancer Res (in press)
  • Peer Reviewed
• [Journal Article] Overexpression of Aurora a by loss of CHFR gene expresion increases the growth of HTLV-1-infected T cells through enhanced NF-kB activity (2009)
  • Author(s): Tomita M, Toyota M, Ishikawa C, Nakazato T, Okudaira T, Matsuda T, Uchihara J, Taira N, Ohshiro K, Senba M, Tanaka Y, Ohshima K, Saya H, Tokino T, Mori N.
  • Journal Title: Int J Cancer 124 Pages: 2607-2615
  • Peer Reviewed
• [Journal Article] p53 family members regulate the expression of the apolipoprotein D gene (2009)
  • Author(s): Sasaki Y, Negishi H, Koyama R, Anbo N, Ohori K, Idogawa M, Mita H, Toyota M, Imai K, Shinomura Y, Tokino T.
  • Journal Title: J Biol Chem 284 Pages: 872-883
  • Peer Reviewed
• [Journal Article] Inflammation-related aberrant patterns of DNA methylation : detection and role in epigenetic deregulation of cancer cell transcriptome (2009)
  • Author(s): Suzuki H, Toyota M, Kondo Y, Shinomura Y.
  • Journal Title: Methods Mol Biol 512 Pages: 55-69
  • Peer Reviewed
• [Journal Article] The Epigenome of colorectal cancer (2009)
  • Author(s): Toyota M, Suzuki H, Shinomura Y.
  • Journal Title: Current Colorectal Cancer Reports 5 Pages: 84-89
  • Peer Reviewed
• [Journal Article] Cancer Epigenomics : Implications of DNA methylation in personalized therapy (2009)
  • Author(s): Toyota M, Suzuki H, Yamashita T, Hirata K, Shinomura Y, Tokino T, Imai K.
  • Journal Title: Cancer Sci 100 Pages: 787-791
  • Peer Reviewed
• [Journal Article] Cancer Epigenomics : Implications of DNA methylation in personalized therapy. (2009)
  • Author(s): Toyota M., et al.
  • Journal Title: Cancer Sci. 100 Pages: 787-791
  • Peer Reviewed
• [Journal Article] The Epigenome of colorectal cancer. (2009)
  • Author(s): Toyota M., et al.
  • Journal Title: Curr. Colorectal Cancer Rep. 5 Pages: 84-89
  • Peer Reviewed
• [Journal Article] Sensitive and specific detection of early gastric cancer using DNA methylation analysis of gastric washing. (2009)
  • Author(s): Watanabe Y., et al.
  • Journal Title: Gastroenterol 136(In press)
  • Peer Reviewed
• [Journal Article] Overexpression of Aurora a by loss of CHFR gene expresion increases the growth of HTLV-1-infected T cells through enhanced NF-kB activity. (2009)
  • Author(s): Tomita M., et al.
  • Journal Title: Int J Cancer 124(In press)
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of microRNA-34b/c and BTG4 is associated with CpG island methylation in colorectal cancer (2008)
  • Author(s): Toyota M, Suzuki H, Sasaki Y, Maruyama R, Imai K, Shinomura Y, Tokino T.
  • Journal Title: Cancer Res 68 Pages: 4123-4132
  • Peer Reviewed
• [Journal Article] DNA hypermethylation contributes incomplete synthesis of carbohydrate determinants in gastrointestinal cancer (2008)
  • Author(s): Kawamura YI, Toyota M, Kawashima R, Hagiwara T, Suzuki H, Imai K, Shinomura Y, Tokino T, Kannagi R, Dohi T.
  • Journal Title: Gastroenterol 135 Pages: 142-151
  • Peer Reviewed
• [Journal Article] A requirement for DICER to maintain full promoter CpG Island hypermethylation in human cancer cells (2008)
  • Author(s): Ting A, Suzuki H, Cope L, Schuebel K, Lee B, Toyota M, Imai K, Shinomura Y, Tokino T, Baylin SB.
  • Journal Title: Cancer Res 68 Pages: 2570-2575
  • Peer Reviewed
• [Journal Article] Identification of flotillin-2, a major protein on lipid rafths, as a novel target of the p53 family members (2008)
  • Author(s): Sasaki Y, Ohshima Y, Koyama R, Maruyama R, Akashi H, Mita H, Toyota M, Shinomura Y, Imai K, Tokino T.
  • Journal Title: Mol Cancer Res 6 Pages: 395-406
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of RASSF2 in oral squamous cell carcinoma (2008)
  • Author(s): Imai T, Toyota M, Suzuki H, Akino K, Ogi K, Sogabe Y, Kashima L, Maruyama R, Nojima M, Mita H, Sasaki Y, Itoh F, Imai K, Shinomura Y, Hiratsuka H, Tokino T.
  • Journal Title: Cancer Sci 99 Pages: 958-966
  • NAID: 10024006089
  • Peer Reviewed
• [Journal Article] Cytoplasmic RASSF2A is a proapoptotic mediator whose expression is epigenetically silenced in gastric cancer (2008)
  • Author(s): Maruyama R, Akino K, Toyota M, Suzuki H, Imai T, Ohe-Toyota M, Yamamoto E, Nojima M, Fujikane T, Sasaki Y, Yamashita T, Watanabe Y, Hiratsuka Y, Hirata K, Itoh F, Imai K, Shinomura Y, Tokino T.
  • Journal Title: Carcinogenesis 29 Pages: 1312-1318
  • Peer Reviewed
• [Journal Article] The hsitone deacetylase inhibitor FK288 enhances adenovirus-mediated p53 family gene therapy in cancer models (2008)
  • Author(s): Sasaki Y, Negishi H, Idogawa M, Suzuki H, Mita H, Toyota M, Shinomura Y, Imai K, Tokino T.
  • Journal Title: Mol Cancer Ther 7 Pages: 779-787
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of SFRP genes in oral squamous cell carcinoma (2008)
  • Author(s): Sogabe Y, Suzuki H, Toyota M, Ogi K, Imai T, Nojima M, Sasaki Y, Hiratsuka H, Tokino T.
  • Journal Title: Int J Oncol 32 Pages: 1253-1261
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of SFRP genes in hepatocellular carcinoma (2008)
  • Author(s): Takagi H, Sasaki S, Suzuki H, Toyota M, Maruyama R, Nojima M, Yamamoto H, Omata M, Tokino T, Imai K, Shinomura Y.
  • Journal Title: J. Gastroenterol 43 Pages: 378-389
  • NAID: 10021268741
  • Peer Reviewed
• [Journal Article] LINE-1 hypomethylation is associated with increased CpG island methylation in helicobactor pylori-related enlarged fold gastritis (2008)
  • Author(s): Yamamoto E, Toyota M, Suzuki H, Kondo Y, Imai K, Sanomura T, Murayama Y, Ohe-Toyota M, Maruyama R, Nojima M, Ashida M, Fujii K, Sasaki Y, Hayashi N, Mori M, Imai K, Tokino T, Shinomura Y.
  • Journal Title: Cancer Epidemiol Biomarkers Prev 17 Pages: 2555-2564
  • Peer Reviewed
• [Journal Article] DNA methylation and cancer pathways in gastrointestinal tumors (2008)
  • Author(s): Suzuki H, Tokino T, Shinomura Y, Imai K, Toyota M.
  • Journal Title: Pharmacogenomics 9 Pages: 1917-1928
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of Wnt antagonist genes in breast cancer (2008)
  • Author(s): Suzuki H, Toyota M, Caraway H, Gabrielson E, Ohmura T, Fujikane T, Nishikawa N, Sogabe Y, Nojima M, Sonoda T, Mori M, Hirata K, Imai K, Shinomura Y, Baylin SB, Tokino T.
  • Journal Title: British J Cancer 98 Pages: 1147-1156
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of secreted Frizzled-related proteins in acute myeloid leukaemia (2008)
  • Author(s): Jost E, Schmid J, Wilop S, Schubert C, Suzuki H, Herman JG, Osieka R, Galm O.
  • Journal Title: Br. J. Haematol 142 Pages: 745-753
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of microRNA-34b/c and BTG4 is associated with CpG island methylation in colorectal cancer. (2008)
  • Author(s): Toyota M., et al.
  • Journal Title: Cancer Res. 68 Pages: 4123-4132
  • Peer Reviewed
• [Journal Article] DNA hypermethylation contributes incomplete synthesis of carbohydrate determinants in gastrointestinal cancer. (2008)
  • Author(s): Kawamura YI., et al.
  • Journal Title: Gastroenterol. 135 Pages: 142-151
  • Peer Reviewed
• [Journal Article] A requirement for DICER to maintain full promoter CpG Island hypermethylation in human cancer cells. (2008)
  • Author(s): Ting A., et al.
  • Journal Title: Cancer Res. 68 Pages: 2670-2675
  • Peer Reviewed
• [Journal Article] Cytoplasmic RASSF2A is a proapoptotic mediator whose expression is epigenetically silenced in gastric cancer. (2008)
  • Author(s): Maruyama R., et al.
  • Journal Title: Care inogenes is 29 Pages: 1312-1318
  • Peer Reviewed
• [Journal Article] The hsitone deacetylase inhibitor FK288 enhances adenovirus-mediated p53 family gene therapy in cancer models. (2008)
  • Author(s): Sasaki Y., et al.
  • Journal Title: Mol. Cancer Ther. 7 Pages: 779-787
  • Peer Reviewed
• [Journal Article] DNA raethylation and cancer pathways in gastrointestinal tumors. (2008)
  • Author(s): Suzuki H., et al.
  • Journal Title: Pharmacogenomics 9 Pages: 1917-1928
  • Peer Reviewed
• [Journal Article] Identification of flotillin-2, a major protein on lipid rafths, as a novel target of the p53 family members. (2008)
  • Author(s): Sasaki Y., et al.
  • Journal Title: Mol. Cancer Res. 6 Pages: 395-406
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of SFRP genes in hepatocellular carcinoma. (2008)
  • Author(s): Takagi H., et al.
  • Journal Title: J. Gastroenterol. 43 Pages: 378-389
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of Wnt antagonist genes in breast cancer. (2008)
  • Author(s): Suzuki H., et al.
  • Journal Title: Br. J. Cancer 98 Pages: 1147-1156
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of RASSF2 in oral squamous cell carcinoma. (2008)
  • Author(s): Imai T., et al.
  • Journal Title: Cancer Sci. 99 Pages: 958-966
  • Peer Reviewed
• [Journal Article] LINE-1 hypomethylation is associated with increased CpG island methylation in helicobactor pylori-related enlarged fold gastritis. (2008)
  • Author(s): Yamamoto E., et al.
  • Journal Title: Cancer Epidemiol. Biomarker. Prev. 17 Pages: 2555-2564
  • Peer Reviewed
• [Journal Article] Identification of flotillin-2, a major protein on lipid rafths, as a novel target of the p53 family members. (2008)
  • Author(s): Sasaki Y
  • Journal Title: Mol Cancer Res. 6 Pages: 395-406
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of Wnt antagonist genes in breast cancer. (2008)
  • Author(s): Suzuki H
  • Journal Title: Brit J. Cancer 98 Pages: 1147-1156
  • Peer Reviewed
• [Journal Article] Cytoplasmic RASSF2A is a proapoptotic mediator whose expression is epigenetically silenced in gastric cancer. (2008)
  • Author(s): Maruyama R
  • Journal Title: Carcinogenesis 29(In press)
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of RASSF2 in oral squamous cell carcinoma. (2008)
  • Author(s): Imai T
  • Journal Title: Cancer Sci 99(In press)
  • Peer Reviewed
• [Journal Article] Integrated genetic and epigenetic analysis identifies colon cancer corresponding to three different subclasses of disease (2007)
  • Author(s): Shen L, Toyota M, Kondo Y, Lin E, Zhang L, Guo Y, Hernandez N, Chen X, Ahmed S, Konishi K, Hamilton SR, Issa JPJ.
  • Journal Title: Proc Natl Acad Sci U S A 104 Pages: 18654-18659
  • Peer Reviewed
• [Journal Article] Gene amplification and overexpression of PRDM14 in breast cancer (2007)
  • Author(s): Nishikawa N, Toyota M, Suzuki H, Homma. T, Fujikane T, Ohmura T, Nishidate T, Ohe-Toyota M, Maruyama R, Sonoda T, Sasaki Y, Urano T, Imai K, Hirata K, Tokino T.
  • Journal Title: Cancer Res 67 Pages: 9649-9657
  • Peer Reviewed
• [Journal Article] Comparing the DNA hypermethylation with gene mutations in human colorectal cancer (2007)
  • Author(s): Schuebel KE, Chen E, Cope Lm Glockner SC, Suzuki H, Yi JM, Chan TA, Van Neste L, Van Criekinge W, van den Bosch S, van Engeland M, Ting AH, Jair K, Yu W, Toyota M, Imai K, Ahuja N, Herman JG, Baylin SB.
  • Journal Title: Plos Genet 3 Pages: 1709-1723
  • Peer Reviewed
• [Journal Article] Identification of DFNA5 as a target of epigenetic inactivation in gastric cancer (2007)
  • Author(s): Akino K, Toyota M, Suzuki H, Imai T, Maruyama R, Kusano M, Nishikawa N, Watanabe Y, Sasaki Y, Abe T, Yamamoto E, Tarasawa I, Sonoda T, Mori M, Imai K, Shinomura Y, Tokino T.
  • Journal Title: Cancer Sci 98 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Antitumor effect of adenoviras-mediated p53 family gene transfer on osteosarcoma cell lines (2007)
  • Author(s): Ohshima Y, Sasaki Y, Negishi H, Idogawa M, Toyota M, Yamashita T, Wada T, Nagoya S, Kawaguchi S, Yamashita T, Tokino T.
  • Journal Title: Cancer Biol Ther 6 Pages: 1058-1066
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of SFRP genes and constitutive activation of Wnt signaling in gastric cancer (2007)
  • Author(s): Nojima M, Suzuki H, Toyota M, Watanabe Y, Maruyama R, Sasaki S, Sasaki Y, Mita H, Nishikawa N, Yamaguchi K, Hirata K, Itoh F, Tokino T, Mori M, Imai K, Shinomura Y.
  • Journal Title: Oncogene 26 Pages: 4699-4713
  • NAID: 130004927598
  • Peer Reviewed
• [Journal Article] Expression profiling of PRDM family genes identified PRDM5 as a target of epigenetic silencing in colorectal and gastric cancer (2007)
  • Author(s): Watanabe Y, Toyota M, Kondo Y, Suzuki H, Imai T, Ohe-Toyota M, Maruyama R, Nojima M, Sasaki Y, Sekido Y, Hiratsuka H, Shinomura Y, Imai K, Itoh F, Tokino T.
  • Journal Title: Clin Cancer Res 13 Pages: 4786-4794
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of DICKKOPF family genes in human gastrointestinal tumors (2007)
  • Author(s): Sato H, Suzuki H, Toyota M, Nojima M, Maruyama R, Sasaki S, Takagi H, Sogabe Y, Sasaki Y, Idogawa M, Sonoda T, Mori M, Imai K, Tokino T, Shinomura Y.
  • Journal Title: Carcinogenesis 28 Pages: 2459-2466
  • NAID: 130004436413
  • Peer Reviewed
• [Journal Article] Gene amplification and overexpression of PRDM14 in breastcancer. (2007)
  • Author(s): Nishikawa N
  • Journal Title: Cancer Res 67 Pages: 9649-9657
  • Peer Reviewed
• [Journal Article] Expression profiling of PRDM family genes identified PRDM5 asa target of epigenetic silencing in colorectal and gastric cancer. (2007)
  • Author(s): Watanabe Y
  • Journal Title: Clin Cancer Res 13 Pages: 4786-4794
  • Peer Reviewed
• [Journal Article] Integrated genetic and epigenetic analysis identifies colon cancer corresponding to three different subclasses of disease. (2007)
  • Author(s): Shen L
  • Journal Title: Proc Natl Acad Sci USA. 104 Pages: 18654-18659
  • Peer Reviewed
• [Journal Article] Comparing the DNA hypermethylation with gene mutations in human colorectal cancer. (2007)
  • Author(s): Schuebel KE
  • Journal Title: Plos Genet. 3 Pages: 1709-1723
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of DICKKOPF family genes in human gastrointestinal tumors. (2007)
  • Author(s): Sato H
  • Journal Title: Carcinogenesis 28 Pages: 2459-2466
  • Peer Reviewed
• [Journal Article] Frequent epigenetic inactivation of SFRP genes and constitutive activation of Wnt signaling in gastric cancer. (2007)
  • Author(s): Nojima M
  • Journal Title: Oncogene 26 Pages: 4699-4713
  • Peer Reviewed
• [Journal Article] Identification of DFNA5 as a target of epigenetic inactivation in gastric cancer. (2007)
  • Author(s): Akino K
  • Journal Title: Cancer Sci. 98 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Antitumor effect of adenoviras-mediated p53 family gene transfer on osteosarcoma cell lines. (2007)
  • Author(s): Ohshima Y
  • Journal Title: Cancer Biol Ther. 6 Pages: 1058-1066
  • Peer Reviewed
• [Presentation] 新規エピゲノム法の開発と消化器癌発生の分子機構. (2009)
  • Author(s): 豊田 実, 他
  • Organizer: 第5回日本消化管学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-02-12
• [Presentation] The role of epigenetic changes in cancer immunothelapy (2009)
  • Author(s): Toyota M, Imai K.
  • Organizer: US-Japan Workshop on Immunological Molecular Markers in Oncology
  • Place of Presentation: Hawaii, USA
• [Presentation] 新規エピゲノム法の開発と消化器癌発生の分子機構 (2009)
  • Author(s): 豊田実、鈴木拓、山本英一郎、山野泰穂、篠村恭久
  • Organizer: 第5回日本消化管学会総会、シンポジウム
  • Place of Presentation: 東京
• [Presentation] 大腸癌のシグナル経路異常におけるDNAメチル化の役割 (2008)
  • Author(s): 豊田 実, 他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Epigenetic gene silencing and microRNA in human colon cancer. (2008)
  • Author(s): Toyota M, et al.
  • Organizer: 36th Congress of the International Societyfor Oncodevelopmental Biology and Medicine
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-09
• [Presentation] The role of DNA methylation changes in signaling pathway. (2008)
  • Author(s): Toyota M, et al.
  • Organizer: AACR Special Conference on Cancer Research : Cancer Epigenetics
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-05-28
• [Presentation] 癌におけるエピジェネティックな異常の網羅的解析 (2008)
  • Author(s): 豊田実、鈴木拓、篠村恭久、時野隆至
  • Organizer: 第31回日本分子生物学会第81回日本生化学会合同年会、シンポジウム
  • Place of Presentation: 神戸
• [Presentation] 大腸癌のシグナル経路異常におけるDNAメチル化の役割 (2008)
  • Author(s): 豊田実、鈴木拓、篠村恭久、時野隆至、今井浩三
  • Organizer: 第67回日本癌学会総会、シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] Epigenetic gene silencing and microRNA in human colon cancer (2008)
  • Author(s): Toyota M.
  • Organizer: Symposium, 36th Congress of the International Society for Oncodevelopmental Biology and Medicine
  • Place of Presentation: Tokyo
• [Presentation] The role of DNA methylation changes in signaling pathway (2008)
  • Author(s): Toyota M.
  • Organizer: AACR Special Conference on Cancer Research : Cancer Epigenetics
  • Place of Presentation: Boston, USA
• [Presentation] Epigenetic alterations in gastrointestinal cancer: functional consequences and clinical application. (2007)
  • Author(s): Toyota M
  • Organizer: IARC Cancer Epigenetics Meeting
  • Place of Presentation: Lyon, France
  • Year and Date: 2007-12-06
• [Presentation] 消化器癌におけるエピジェネティックな異常の解析と臨床応用 (2007)
  • Author(s): 豊田実
  • Organizer: 日本消化器病学会東海支部第107回例会・第18回教育講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-11-17
• [Presentation] 慢性胃炎におけるエピジェネティックな異常-DNAメチル化のおこるプロセス- (2007)
  • Author(s): 豊田 実
  • Organizer: 第49回日本消化器病学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2007-10-21
• [Presentation] The role of DNA methylation changes in signal transduction in gastric cancer. (2007)
  • Author(s): 豊田 実
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-10-05
• [Presentation] Epigenetic silencing of genes involved in signaling pathways in gastric cancer. (2007)
  • Author(s): Toyota M
  • Organizer: 35th Congress of the International Society for Oncodevelopmental Biology and Medicine
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2007-09-18
• [Presentation] Epigenetic alterations in gastrointestinal cancer : functional consequences and clinical application (2007)
  • Author(s): Toyota M.
  • Organizer: IARC Cancer Epigenetics Meeting
  • Place of Presentation: Lyon, France
• [Presentation] 慢性胃炎におけるエピジェネティックな異常-DNAメチル化のおこるプロセス- (2007)
  • Author(s): 豊田実、山本英一郎、篠村恭久
  • Organizer: 第49回日本消化器病学会大会
  • Place of Presentation: 神戸
• [Presentation] The role of DNA methylation changes in signal transduction in gastric cancer (2007)
  • Author(s): 豊田実、鈴木拓、今井浩三、時野隆至、篠村恭久
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Epigenetic silencing of genes involved in signaling pathways in gastric cancer (2007)
  • Author(s): Toyota M.
  • Organizer: Symposium, 35th Congress of the International Society for Oncodevelopmental Biology and Medicine
  • Place of Presentation: Plaque, Czech Republic
• [Book] CHFR as a potential anticancer target. Cancer Drug Discovery and Development Checkpoint Response in Cancer Therapy (2008)
  • Author(s): Toyota M, Kashima L, Tokino T.
  • Total Pages: 314
  • Publisher: Edited by Dai W, Humana Press, NJ, USA
• [Book] エピジェネティクス-メチル化とヒストン修飾. がんの分子標的治療.(鶴尾隆. 編集.) (2008)
  • Author(s): 今井浩三、豊田実
  • Publisher: 南山堂
• [Book] 消化管と遺伝子異常. 消化管病理標本の読み方改訂2版.(中村眞一. 編集.) (2008)
  • Author(s): 豊田実、今井浩三、篠村恭久
  • Publisher: 日本メディカルセンター
• [Book] 消化管病理標本の読み方改訂2版. (2008)
  • Author(s): 豊田 実, 他
  • Total Pages: 228
  • Publisher: 日本メディカルセンター
• [Remarks] 豊田実. DNA methylation as a new target for therapy in cancer. 日本癌学会2007年度JCA-Mauverney Award. 2007.
• [Patent(Industrial Property Rights)] 癌の検出方法および検出用キット、ならびに癌治療剤 (2008)
  • Industrial Property Number: 2008-121671
  • Filing Date: 2008-05-07
• [Patent(Industrial Property Rights)] 癌の検出方法および検出用キット、ならびに癌治療剤. (2008)
  • Inventor(s): 豊田 実, 他4名
  • Industrial Property Rights Holder: 札幌医科大学
  • Industrial Property Number: 2008-121671
  • Filing Date: 2008-05-07