T helper 2 type cytokines in the gastrointestinal tissue injury

• Project/Area Number: 19390204
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: DOHI Taeko Research Institute, International Medical Center of Japan, 部長 (60250221)
• Co-Investigator(Kenkyū-buntansha): IZUHARA Kenji 佐賀大学, 医学部 (00270463) ; ATSUSHI Nishikawa 東京農工大学, 大学院・共生科学技術研究院, 教授 (30218127) ; KAWAMURA Yuki 独立行政法人国立国際医療研究センター (10392391)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2007: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: IL-13 / IL-4 / アポトーシス / 上皮細胞 / 消化管上皮細胞

Research Abstract

IL-13 induced injury in the gastrointestinal epithelial cells. We proved that this effect did not required the presence of mesenchymal cells nor IL-4 receptor α,but was direct effect of IL-13 on epithelial cells. We found the IL-13 induced apoptosis in the epithelial cells by activating caspases 3, 8, and 9. TNF-α was involved in the IL-13 induced apoptosis.

Research Products

• [Journal Article] TWEAK/Fn14 Pathway: a nonredundant role in intestinal damage in mice through a TWEAK/intestinal epithelial cell axis. (2009)
  • Author(s): Dohi T, Borodovsky A, Wu P, Shearstone JR, Kawashima R, Runkel L, Rajman L, Dong X, Scott ML, Michaelson JS, Jakubowski A, Burkly LC
  • Journal Title: Gastroenterology 136 Pages: 912-923
• [Journal Article] TWEAK/Fn14 Pathway : a nonredundant role in intestinal damage in mice through a TWEAK/intestinal epithelial cell axis (2009)
  • Author(s): Dohi, T
  • Journal Title: Gastroenterology 136 Pages: 912-923
  • Peer Reviewed
• [Journal Article] インターロイキン13と上皮細胞バリア (2009)
  • Author(s): 土肥多惠子
  • Journal Title: 臨床免疫・アレルギー科 52 Pages: 344-348
• [Journal Article] TWEAK/Fn14経路の炎症と組織修復における意義 (2009)
  • Author(s): 土肥多惠子
  • Journal Title: 感染・炎症・免疫 39 Pages: 62-64
• [Journal Article] TWEAK/Fn14 Pathway : a nonredundant role in intestinal damage in mice through a TWEAK/intestinal epithelial cell axis (2009)
  • Author(s): T. Dohi
  • Journal Title: Gastroenterology 136 Pages: 912-923
  • Peer Reviewed
• [Journal Article] DNA hypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer (2008)
  • Author(s): Kawamura, YI, Toyota M, Kawashima R, Hagiwara T, Suzuki H, Imai K, Shinomura Y, Tokino T, Kannagi R, Dohi T
  • Journal Title: Gastroenterology 135 Pages: 142-151
• [Journal Article] Incomplete synthes0is of the Sd(a)/Cad blood group carbohydrate in gastrointestinal cancer. (2008)
  • Author(s): Dohi T, Kawamura YI
  • Journal Title: Biochim Biophys Ada 1780 Pages: 467-471
• [Journal Article] DNA hypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer (2008)
  • Author(s): Y. I. Kawamura
  • Journal Title: Gastroenterology 135 Pages: 142-151
  • Peer Reviewed
• [Journal Article] DNAhypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer (2008)
  • Author(s): Y.I. Kawamura
  • Journal Title: Gastroenterology (In press)
  • Peer Reviewed
• [Journal Article] Incomplete synthesis of the Sd(a)/Cad blood group carbohydrate in gastrointestinal cancer. (2008)
  • Author(s): T.Dohi
  • Journal Title: Biochim Biophys Acta 1780 Pages: 467-471
  • Peer Reviewed
• [Journal Article] Dose-dependent, differential regulation of cytokine secretion from macrophages by fractalkine. (2007)
  • Author(s): Mizutani N, Sakurai T, Shibata T, Uchida K, Fujita J, Kawashima R, Kawamura Y.I., Toyama-Sorimachi N, Imai T, Dohi T.
  • Journal Title: J. Immunol 79 Pages: 7478-7487
• [Journal Article] Dose-dependent differential regulation of cytokine secretion from macrohages by fractalkine. (2007)
  • Author(s): Mizutani N
  • Journal Title: J Immunol 79 Pages: 7478-7487
  • Peer Reviewed
• [Presentation] TWEAK/Fn14 Pathway Promotes Epithelial Cell Apoptosis after g-irradiation. (2009)
  • Author(s): Dohi T, Kawashima R, Wu P, Burkly LC
  • Organizer: Internatinal Conference of Mucosal Immunity 2009
  • Place of Presentation: Boston
  • Year and Date: 2009-07-06
• [Presentation] TWEAK/Fn14 Pathway Promotes Epithelial Cell Apoptosis after γ-irradiation (2009)
  • Author(s): Dohi T
  • Organizer: International Conference of Mucosal Immunity 2009
  • Place of Presentation: Boston
  • Year and Date: 2009-07-06
• [Presentation] Kawashima R, Burkly LC. Role of TWEAK (TNF-α-Like Weak Inducer of Apoptosis) in Intestinal Inflammation and Tissue Repair. (2009)
  • Author(s): Dohi T
  • Organizer: Selected as Poster of distinction, Digestive Disease Week 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-05-31
• [Presentation] Role of TWEAK(TNF-α-Like Weak Inducer of Apoptosis)in Intestinal Inflammation and Tissue Repair (2009)
  • Author(s): Dohi T
  • Organizer: Digestive Disease Week 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-03-31
• [Presentation] Inflammation-associated transcriptional silencing of Sda carbohydrate-synthase gene by DNA hypermethylation in ulcerative colitis. (2008)
  • Author(s): Kawamura YI, Toyota M, Kawashima R, Hagiwara T, Kawamura YJ, Konishi F, Saito Y, Kannagi R, Imai K, Dohi T.
  • Organizer: Biochemistry and Molecular Biology (BMB) 2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Inflammation-associated transcriptional silencing of Sda carbohydrate-synthase gene by DNA hypermethylation in ulcerative colitis (2008)
  • Author(s): Y I. Kawamura
  • Organizer: Biochemistry and Molecular Biology (BMB) 2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] IL-13 disrupts tight junction in the intestinal epithelial cells by modulating expression of ZO-1, ocdudin and claudin-2. (2008)
  • Author(s): Kawashima R, Kawamura YI. Toayama-Sorimachi N, Dohi T
  • Organizer: 第38回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] IL-13 disrupts tight junction in the intestinal epithelial cells by modulating expression of ZO-1, occludin and claudin-2 (2008)
  • Author(s): Kawashima R
  • Organizer: 第38回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Comprehensive analysis of lectin-binding in the colitis and colitis-associated tumors in mice. (2008)
  • Author(s): V Phongsisay, Y I. Kawamura, R Kawashiama, T Dohi
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Comprehensive analysis of lectin-binding in the colitis and colitis-associated tumors in mice (2008)
  • Author(s): Vongsavanh P
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Interleukin-13 Induces Tissue Damage with Relocation ofβ-Catenin and Modification of Cell-Cell Adhesion in the Epithelial Cells. (2008)
  • Author(s): Kawashima R, Kawamura YI, Mizutani N, Toyama-Sorimachi N, Dohi T
  • Organizer: Digestive Disease Week 2008, Selected as Poster of distinction
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2008-05-20
• [Presentation] Tweak/Fn14 Pathway: Role in the Intestinal Inflammation and Tissue Repair. (2008)
  • Author(s): Dohi T, Borodovsky A, Kawashima R, Wu P, Kawamura YI, Burkly LC
  • Organizer: Digestive Disease Week 2008, Selected as Poster of distinction
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2008-05-18
• [Presentation] Interleukin-13 Induces Tissue Damage with Relocation of β-Catenin and Modification of Cell-Cell Adhesion in the Epithelial Cells (2008)
  • Author(s): Kawashima R
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego, U. S. A.
  • Year and Date: 2008-05-18
• [Presentation] Tweak/Fn14 Pathway : Role in the Intestinal Inflammation and Tissue Repair (2008)
  • Author(s): Dohi T
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego, U. S. A.
  • Year and Date: 2008-05-18
• [Presentation] Interleukin-13 disrupts the cell-cell adhesion of intestinal epithelial cells (2007)
  • Author(s): Kawashima R, Kawamura YI, Mizutani N, Toyama-Sorimachi N Dohi T
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21
• [Presentation] IL-13作用の調節による消化管上皮細胞の再生促進 (2007)
  • Author(s): 土肥多恵子
  • Organizer: 第49回日本消化器病学会大会(JDDW)
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-19
• [Presentation] Silencing of Sda carbohydrate-synthase gene by aberrant methylation in gastrointestinal cancer and ulcerative colitis (2007)
  • Author(s): Kawamura YI, Toyota M, Kawashima R, Hagiwara T, Kawamura YJ, Konishi F, Takeshita E, Miyake O, Saito Y, Kannagi R, T Dohi
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo
  • Year and Date: 2007-07-10
• [Presentation] Silencing of Sd^a carbohydrate-synthase gene by aberrant methylation in gastrointestinal cancer and ulcerative colitis (2007)
  • Author(s): Kawamura YI
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo
  • Year and Date: 2007-07-10
• [Presentation] 消化管炎症における免疫応答と組織修復 (2007)
  • Author(s): 土肥多恵子
  • Organizer: 神戸免疫アレルギー談話会
  • Place of Presentation: 神戸
  • Year and Date: 2007-06-14
• [Presentation] Fractalkine Regulates TNF-alfa secretion by macrophages with induction of peroxisome proliferator-activated receptor-gamma and its ligand (2007)
  • Author(s): Mizutani N, Sakurai T, Shibata T, Uchida K, Fujita J, Kawashima R, Kawamura YI, Toyama-Sorimachi N, Imai T, Dohi T
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington D.C.
  • Year and Date: 2007-05-23
• [Presentation] Fractalkine Regulates TNF-α secretion by macrophages with induction of peroxisome proliferator-activated receptor-gamma and its ligand (2007)
  • Author(s): Mizutani N
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington D.C
  • Year and Date: 2007-05-23
• [Presentation] Aberrant responses of human colonic macrophage-type cells to lipopolysaccharide in ulcerative colitis: upregulated expression of MD-2 and production of Inflammatory cytokines (2007)
  • Author(s): Kawashima R, Kawamura YI, Mizutani N, Toyama-Sorimachi N, Saito Y, Kawamura YJ, Fumio, Konishi, Dohi T
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington D.C.
  • Year and Date: 2007-05-22
• [Presentation] Aberrant responses of human colonic macrophage-type cells to lipopolysaccharide in ulcerative colitis:upregulated expression of MD-2 and production of Inflammatory cytokines (2007)
  • Author(s): Kawashima R
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington D.C
  • Year and Date: 2007-05-22