Basic analysis of new collectins in renal tract.

• Project/Area Number: 19390227
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: WAKAMIYA Nobutaka Asahikawa Medical College, 医学部・医学科, 教授 (20210867)
• Co-Investigator(Kenkyū-buntansha): OHTANI Katsuki 旭川医科大学, 医学部, 助教 (90396367) ; SUZUKI Ysuhiko 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90206540) ; YOHIDA Itsuro 旭川医科大学, 医学部, 准教授 (20041816) ; MOTOMURA Wataru 旭川医科大学, 医学部, 助教 (70374791) ; JANG Seong-jae 旭川医科大学, 医学部, 特任助教 (50466497) ; 福澤 純 旭川医科大学, 医学部, 特任准教授 (40333695)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 腎疾患 / コラーゲン / レクチン

Research Abstract

初年度において、各種リコンビナントコレクチン作成とその抗体作成を行った。遺伝子改変動物の作成については、ノックアウトマウスはCL-P1、CL-K1について両者とも、キメラマウスが樹立でき、さらにヘテロマウス作成が終了し、精子保存にてノックアウト表現型解析に進んでいる。平成22年度末の時点で、CL-P1は、仔マウスとして誕生せず、胎生致死に関与する遺伝子であると考えられた。一方、CL-K1ノックアウトマウスは無事誕生し、一見正常に見えた。しかしながら、ノックアウトマウス個体が野生型の通常マウスに比較して小型であることや、誕生個体が少ないことより、個体発生や生育に関与する遺伝子である可能性がでている。腎組織おける検討では、局在は異なるが両コレクチンの存在することが、本研究により再確認されたが、上記マウスを用いた病態における意義については十分な解析結果が得られていない、今後の検討が待たれる。

Research Products

• [Journal Article] Mannose binding lectin and lung collectins interact with Toll-like receptor 4 and MD-2 by different mechanisms (2009)
  • Author(s): Shimizu, T., Nishitani, C., Mitsuzawa, H., Ariki,S., Takahashi, M., Ohtani, K., Wakamiya, N., Kuroki, Y.
  • Journal Title: B.B.A. 1790 Pages: 1705-1710
• [Journal Article] Scavenger receptor collectin placenta 1(CL-P1)predominantly mediates zymosan phagocytosis by human vascular endothelial cells (2009)
  • Author(s): Jang S-J, Ohtani K, Fukuoh A, Yoshizaki T, Fukuda M, Motomura W, Mori K, Fukuzawa J, Kitamaoto N, Yoshida I, Suzuki Y, Wakamiya, N.
  • Journal Title: J. Biol. Chem. 284(6) Pages: 3956-65
• [Journal Article] Troglitazone increases expression of E-cadherin and claudin 4 in human pancreatic cancer cells (2009)
  • Author(s): Kumei S, Motomura W, Yoshizaki T, Takakusaki K, Okumura T
  • Journal Title: Biochem Biophys Res Commun. 380(3) Pages: 614-9
• [Journal Article] A Jak2 inhibitor, AG490, reverses lipin-1 suppression by TNF-alpha in 3T3-L1 adipocytes (2009)
  • Author(s): Tsuchiya Y, Takahashi N, Yoshizaki T, Tanno S, Ohhira M, Motomura W, Tanno S, Takakusaki K, Kohgo Y, Okumura T.
  • Journal Title: Biochem Biophys Res Commun. 382(2) Pages: 348-52
• [Journal Article] Loop-Mediated Isothermal Amplification (LAMP) for the direct detection of human pulmonary infections with environmental (nontuberclosis) mycobacteria (2009)
  • Author(s): Adhikari BR, Pandy BD, Ghimire P, Shrestha B, Khadka M, Yoda T, Suzuki Y.
  • Journal Title: Jpn. J. Infect. Dis. 62 Pages: 212-214
• [Journal Article] Mannose binding lectin and lung collectins interact with Toll-like receptor 4 and MD-2 by different mechanisms (2009)
  • Author(s): Shimizu, T., Nishitani, C., Mitsuzawa, H., Ariki, S., Takahashi, M., Ohtani, K., Wakamiya, N., Kuroki, Y.
  • Journal Title: Biochimica et Biophysica Acta 1790 Pages: 1705-1710
  • Peer Reviewed
• [Journal Article] Scavenger receptor collectin placenta 1 (CL-P1) predominantly mediates zymosan phagocytosis by human vascular endothelial cells (2009)
  • Author(s): Jang S-J, 大谷克城, Fukuoh A, Yoshizaki T, Fukuda M, Motomura W, Mori K, Fukuzawa J, Kitamaoto N, Yoshida I, Suzuki Y, 若宮伸隆
  • Journal Title: J.Biol.Chem. 284 Pages: 3956-3965
  • Peer Reviewed
• [Journal Article] 新規コレクチンの発見 (2009)
  • Author(s): 若宮伸隆, 大谷克城
  • Journal Title: 旭川医大研究フォーラム 第10巻(印刷中)
  • NAID: 120002055932
• [Journal Article] Immunolocalization of a novel collectin CL-K1 in murine tissues (2008)
  • Author(s): Motomura W, Yoshizaki T, Ohtani K, Okumura T, Fukuda M, Fukuzawa J, Mori K, Jang SJ, Nomura N, Yoshida I, Suzuki Y, KohgoY, Wakamiya, N
  • Journal Title: J Histochem Cytochem 56(3) Pages: 243-52
  • NAID: 120003182905
• [Journal Article] Detection of culturable and nonculturable Legionella species from hot water systems of public buildings in Japan (2008)
  • Author(s): Edagawa A, Kimura A, Doi H, Tanaka H, Tomioka K, Sakabe K, Nakajima C, Suzuki Y.
  • Journal Title: J. Appl. Microbiol. 105 Pages: 2104-2114
• [Journal Article] A novel method to simply detect mutations conferring drug resistance in Mycobacterium leprae based on a DNA microarray, and its applicability in developing countries (2008)
  • Author(s): Matsuoka M, Aye KS, Kyaw K, Tan EV, Balagon MV, Saunderson P, Makino M, Nakajima C, Suzuki Y
  • Journal Title: J. Med. Microbiol. 57 Pages: 1213-1219
• [Journal Article] Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients (2008)
  • Author(s): Pandey BD, Poudel A, Yoda T, Tamaru A, Oda N, Fukushima Y, Lekhak B, Risal B, Acharya B, Sapkota B, Nakajima C, Taniguchi T, Phetsuksiri B, Suzuki Y.
  • Journal Title: J. Med. Microbiol. 57 Pages: 439-43
• [Journal Article] Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily (2008)
  • Author(s): Higuchi M, Matsuo A, Shingai M, Shida K, Ishii A, Funami K, Suzuki Y, Oshiumi H, Matsumoto M, Seya T.
  • Journal Title: Dev. Comp. Immunol. 32 Pages: 147-55
• [Journal Article] 新規コレクチンCL-L1, CL-P1, CL-K1の構造と機能 (2008)
  • Author(s): 鈴木定彦、大谷克城、張成宰、本村亘、若宮伸隆
  • Journal Title: 臨床検査 52 Pages: 861-869
• [Journal Article] Scavenger receptor collectin placenta 1 (CL-P1) predominantlymediates zymosan phagocytosis by human vascular endothelial cells (2008)
  • Author(s): Jang S-J, Ohtani K, Fukuoh A, Yoshizaki T, Fukuda M, Motomura W, Mori K, Fukuzawa J, Kitamaoto N, Yoshlda I, Suzuki Y, Wakamiya N.
  • Journal Title: J. Biol. Chem 284 Pages: 3956-3965
  • Peer Reviewed
• [Journal Article] Immunolocalization of a novel collectin CL-K1 in murine tissues. (2008)
  • Author(s): Motomura W, Yoshizaki T, Ohtani K, Okumura T, Fukuda M, Fukuzawa J, Mori K, Jang SJ, Nomura N, Yoshida I, Suzuki Y, Kohgo Y, Wakamiya, N.
  • Journal Title: J Histochem Cytochem 56(3) Pages: 243-252
  • NAID: 120003182905
  • Peer Reviewed
• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sakamoto T, Ohtani K, Wakamiya, N.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-4
  • NAID: 110006201244
• [Journal Article] Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice (2007)
  • Author(s): Ohhira M, Motomura W, Fukuda M, Yoshizaki T, Takahashi N, Tanno S, Wakamiya, N., Kohgo Y, Kumei S, Okumura T.
  • Journal Title: J Gastroenterol. 42 Pages: 969-78
  • NAID: 10020178695
• [Journal Article] Evaluation and Application of Detecting Norovirus using Reverse Transcription Loop-Mediated Isothermal Amplification (2007)
  • Author(s): Yoda T, Suzuki Y, Yamazaki K, Sakon N, Kanki M, Aoyama I, Tsukamoto T.
  • Journal Title: J. Med. Virol. 79 Pages: 326-334
• [Journal Article] Association of the Rv0679c protein with lipids and carbohydrates in Mycobacterium tuberculosis/Mycobacterium bovis BCG (2007)
  • Author(s): Matsuba T, Suzuki Y, Tanaka Y.
  • Journal Title: Arch. Microbiol 187 Pages: 297-311
• [Journal Article] Anti-influenza A virus activities of mannan-binding lectins and bovine conglutinin. (2007)
  • Author(s): Kawai T, Kase T, Suzuki Y, Eda S, Sak amoto T, Ohtani K, Wakamiya N.
  • Journal Title: J Vet Med Sci. 69(2) Pages: 221-224
  • NAID: 110006201244
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice. (2007)
  • Author(s): Ohhira M, Motomura W, Fukuda M, Yoshizaki T, Takahashi N, Tanno S, Wakamiya N, Kohgo Y, Kumei S, Okumura T.
  • Journal Title: J Gastroenterol. 42(12) Pages: 969-978
  • NAID: 10020178695
  • Peer Reviewed
• [Journal Article] Collagen-related lectins 1-237 (2007)
  • Author(s): Suzuki, Y., Ohtani, K., Wakamiya, N.
  • Journal Title: Collagen-related lectins in innate immunity, Chapter 5: The novel collectins, CL-L1, CL-P1, CL-K1., Research Signpost,
• [Presentation] ドメイン欠損CL-P1のリガンド結合解析 (2009)
  • Author(s): 森健一郎、大谷克城、張成宰、金然旭、本村亘、孫啓輝、吉田逸朗、鈴木定彦、若宮伸隆
  • Organizer: 第46回補体シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2009-08-21
• [Presentation] Molecular Cloning and Characterization of Novel Ascidian Collectins (2009)
  • Author(s): 大谷克城, K Azumi, S-J Jang, 森健一郎, 本村亘, 吉田逸朗, 鈴木定彦, 若宮伸隆
  • Organizer: 11^<th> Congress of the International Society of developmental and Comparative Immunology
  • Place of Presentation: Prague
  • Year and Date: 2009-06-22
• [Presentation] Molecular Cloning and Characterization of Novel Ascidian Collectins (2009)
  • Author(s): K Ohtani, K Azumi, S-J Jang, K Mori, W Motomura, I Yoshida, Y Suzuki, N. Wakamiya, N.
  • Place of Presentation: Prague(Czech Republic)
• [Presentation] Cloning and characterization of novel Ascidian collectins (2008)
  • Author(s): Wakamiya N., et.al.
  • Organizer: INTERLEC-23
  • Place of Presentation: Stirling (Scotland)
  • Year and Date: 2008-07-15
• [Presentation] ヒト血管内皮細胞では酵母ファゴサイトーシスはCL-P1が担う (2008)
  • Author(s): 張成宰、大谷克城、森健一郎、本村亘、福澤純、吉田逸朗、若宮伸隆
  • Organizer: 第45回補体シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-10
• [Presentation] Cloning and characterization of novel Ascidian collectins (2008)
  • Author(s): Wakamiya, N., Ohtani K, Azumi K, Jang S-J, Mori K, Fukuzawa J, Yoshida I, Suzuki Y.
  • Organizer: INTERLEC-23
  • Place of Presentation: Stirling(Scotland)
• [Presentation] 新規コレクチンファミリーの発見とその役割 (2007)
  • Author(s): 若宮伸隆、大谷克城、坂本隆志、芥子宏行、福應温、張成宰、吉崎隆之、小山聡、福澤純、本村亘、吉田逸朗、鈴木定彦
  • Organizer: 第44回補体シンポジウム
  • Place of Presentation: 神奈川県平塚市
  • Year and Date: 2007-08-24
• [Book] The novel collectins, CL-L1, CL-P1 and CL-K1. COLLAGEN-RELATED LECTINS IN INNATE IMMUNITY(Kilpatrick D. edd.)(REVIEW) (2007)
  • Author(s): Suzuki Y, Ohtani K, Wakamiya, N.
  • Publisher: Research Spotlight
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/microbio/microbiology.html
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/microbio/microbiology.html
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/microbio/microbiology.html
• [Remarks]
  • URL: http://www.asahikawa-med.ac.jp/dept/mc/imcrobio/microbiology.html
• [Patent(Industrial Property Rights)] 組換えマンナン結合タンパク質 (2009)
  • Inventor(s): 若宮伸隆
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Acquisition Date: 2009-08-11
  • Overseas
• [Patent(Industrial Property Rights)] 組換えマンナン結合タンパク質 (2009)
  • Inventor(s): 若宮伸隆
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Acquisition Date: 2009-08-11
  • Overseas
• [Patent(Industrial Property Rights)] 新規スカベンジャーレセプター (2009)
  • Inventor(s): 若宮伸隆
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Acquisition Date: 2009-05-06
  • Overseas
• [Patent(Industrial Property Rights)] 新規コレクチン(CL-K1) (2009)
  • Inventor(s): 若宮伸隆, その他4名
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Acquisition Date: 2009-02-17
  • Overseas
• [Patent(Industrial Property Rights)] コレクチン活性を有するhCL-K1ポリペプチド (2007)
  • Inventor(s): 若宮伸隆, その他4名
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Filing Date: 2007-04-05
  • Overseas
• [Patent(Industrial Property Rights)] コレクチン活性を有するhCL-K1ポリペプチド (2007)
  • Inventor(s): 若宮伸隆、その他4名
  • Industrial Property Rights Holder: 扶桑薬品工業株式会社
  • Filing Date: 2007-04-05
  • Overseas