Genomics and proteomics analyses ofpathophysiological mechanism in complications associated with long term hemodialysis therapy

• Project/Area Number: 19390230
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: KAZAMA Junichiro Niigata University, 医歯学総合病院, 講師 (10345499)
• Co-Investigator(Kenkyū-buntansha): NARITA Ichiei 新潟大学, 医歯学系, 准教授 (20272817) ; 山本 卓 新潟大学, 医歯学総合病院, 医員 (70444156)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: ゲノミクス / プロテオミクス / 慢性腎不全 / アミロイドーシス / 透析療法 / 長期透析合併症 / 骨代謝 / 顕微コンピュータートモグラフィー / バーチャル3次元空間解析 / 非侵襲検査 / 透析アミロイドーシス / 胆石症 / 血尿

Research Abstract

長期透析に特有な透析アミロイドーシス、腎性骨症、腎性貧血、動脈石灰化などを対象疾患として、疾患とその修飾因子の両サイドから病因検索や治療応答への影響を検索した。本研究によって透析患者長期生存に及ぼす臨床因子が初めて絞り込まれ、またミネラル代謝に及ぼすリンの影響を初めて定量的に示すことなどの成果を得た。また、この過程に作成した約4000人の維持血液透析患者データベースは、期間内の研究結果を導いたのみならず、今後の前向き研究にも有用な足がかりとなった。期間内にこのプロジェクト関係で出版された英語論文19編、国内学会発表9演題、国際学会発表8演題、出版図書7冊。

Research Products

• [Journal Article] The risk of gall bladder stone formation is increased in patients with predialysis chronic kidney disease but not those undergoing chronic haemodialysis therapy (2009)
  • Author(s): Kazama JJ, Kazama S, Koda R, Yamamoto S, Narita I, Gejyo F
  • Journal Title: Nephron Clin Prac 111
  • Peer Reviewed
• [Journal Article] Microscopic hematuria is associated with low bone mineral density in aged women and men (2009)
  • Author(s): Nakamura K, Kazama JJ, Tanaka Y, Makabe K, Oyama M, Tsuchiya Y, Yamamoto M
  • Journal Title: J Bone Miner Metab 27 Pages: 251-254
  • Peer Reviewed
• [Journal Article] Egg on tha table (2009)
  • Author(s): Kazama JJ, Kaneko Y
  • Journal Title: Clin J Am Soc Nephrol 4 Pages: 14-15
  • Peer Reviewed
• [Journal Article] Comparison of quantitative cancellous bone connectivity analyses in 2- and 3-dimensional levels in dialysis patients (2009)
  • Author(s): Kazama JJ, Koda R, Yamamoto S, Narita I, Gejyo F, Tokumoto A
  • Journal Title: Calc Tis Int 84 Pages: 38-44
  • Peer Reviewed
• [Journal Article] The risk of gall bladder stone formation is increased in patients with predialysis chronic kidney disease but not those undergoing chronic haemodialysis therapy. (2009)
  • Author(s): Kazama JJ, Kazama S, Koda R, et al.
  • Journal Title: Nephron Clin Prac. 111
  • Peer Reviewed
• [Journal Article] Comparison of quantitative cancellous bone connectivity analyses in 2- and 3-dimensional levels in dialysis patients. (2009)
  • Author(s): Kazama JJ, Koda R, Yamamoto S, et al.
  • Journal Title: Calc Tis Int 84 Pages: 38-44
  • Peer Reviewed
• [Journal Article] Patients undergoing dialysis therapy for 30 years or more survive with serious complication of osteoarticular disorder (2008)
  • Author(s): Yamamoto S, Kazama JJ, Maruyama H, Nishi S, Narita I, Gejyo F
  • Journal Title: Clin Nephrol 70 Pages: 496-502
  • Peer Reviewed
• [Journal Article] Toll-like receptor 9 affects severity of IgA nephropathy (2008)
  • Author(s): Suzuki H, Suzuki Y, Narita I, Aizawa M, Kihara M, Yamanaka T, Kanou T, Tsukaguchi H, Novak J, Horikoshi S, Tomino Y
  • Journal Title: J Am Soc Nephrol 19 Pages: 2384-2395
  • Peer Reviewed
• [Journal Article] Serum cystatin C measured by sol particle homogeneous immunoassay can accurately detect early impairment of renal function (2008)
  • Author(s): Sato H, Kazama JJ, Kuroda T, Narita I, Nakano M, Gejyo F
  • Journal Title: Clin Exp Nephrol 12 Pages: 270-276
  • NAID: 10025585347
  • Peer Reviewed
• [Journal Article] Pathogenetic significance of aberrant glycosylation of IgA1 in IgA nephropathy (2008)
  • Author(s): Narita I, Gejyo F
  • Journal Title: Clin Exp Nephrol 12 Pages: 332-338
  • Peer Reviewed
• [Journal Article] Uremic pruitus in chronic hemodialysis patients (2008)
  • Author(s): Narita I, Iguchi S, Omori K, Gejyo F.
  • Journal Title: J Nephrol 21 Pages: 161-165
  • Peer Reviewed
• [Journal Article] Alterations in serum phosphate levels predict the long-term response to intravenous calcitriol therapy in dialysis patients with secondary hyperparathyroidism (2008)
  • Author(s): Hosaka K, Kazama JJ, Yamamoto S, Ito Y, Iino N, Maruyama H, Saito A, Narita I, Gejyo F
  • Journal Title: J Bone Miner Metab 26 Pages: 185-190
  • NAID: 10024126616
  • Peer Reviewed
• [Journal Article] Effects of serum calcium, phosphorous, and intact parathyroid hormone levels on survival in chronic hemodialysis patients in Japan (2008)
  • Author(s): Nakai S, Akiba T, Kazama J, Yokoyama K, Fukagawa M, Tominaga Y, Iseki K, Tsubakihara Y
  • Journal Title: Ther Apher Dial 12 Pages: 49-54
  • Peer Reviewed
• [Journal Article] Alterations in serum phosphate levels predict the long-term response to intraven ous calcitriol therapy in dialysis patients with secondary hyperparathyroidism (2008)
  • Author(s): Hosaka K, Kazama JJ, Yamamoto S, et al.
  • Journal Title: J Bone Miner Metab 26 Pages: 185-190
  • Peer Reviewed
• [Journal Article] Serum cystatin C measured by sol particle homogeneous immunoassay can accurately detect early impairment of renal function. (2008)
  • Author(s): Sato H, Kazama JJ, Yamamoto s, et al.
  • Journal Title: Clin Exp Nephrol. 12 Pages: 270-276
  • Peer Reviewed
• [Journal Article] Patients undergoing dialysis therapy for 30 years or more survive with serious complication of osteoarticular disorder. (2008)
  • Author(s): Yamamoto s, Kazama JJ, MaruyamaH, et al.
  • Journal Title: Clin Nephrol 70 Pages: 496-502
  • Peer Reviewed
• [Journal Article] Microscopic hematuria is associated with low bone mineral density in aged women and men (2008)
  • Author(s): Nakamura K, Kazama JJ, Tanaka Y, et al.
  • Journal Title: J Bone Miner Metab 26 Pages: 185-190
  • Peer Reviewed
• [Journal Article] Alteration in serum phosphate levels predict the long-term response to intravenous calcitriol therapy in dialysis patients with secondary hyperparathyroidism. (2008)
  • Author(s): Hosaka K, et. al.
  • Journal Title: Journal of Bone and Mineral Metabolism 26・2 Pages: 185-190
• [Journal Article] Effects of serum calcium, phosphorous, and intact parathyroid hormone levels on survival in chronic hemodialysis patients in Japan. (2008)
  • Author(s): Nakai S, et. al.
  • Journal Title: Therapeutic Apheresis and Dialysis 12・1 Pages: 49-54
• [Journal Article] Japanese Society of Dialysis Therapy Treatment Guidelines for Secondary Hyperparathyroidism (2007)
  • Author(s): Kazama JJ
  • Journal Title: Therp Apher Dial 11(Supp1) Pages: 44-47
  • Peer Reviewed
• [Journal Article] What does the circulating AHSG/fetuin-A level tell us? (2007)
  • Author(s): Kazama JJ
  • Journal Title: Clin Exp Nephrol 11 Pages: 336-337
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor (FGF)-23 in patients with transient hypoparathyroidisn : Its important role in serum phosphate regulation (2007)
  • Author(s): Yamashita H, Yamazaki Y, Hasegawa H, Yamashita T, Fukumoto S, Shigematsu T, Kazama JJ, Fukagawa M, Noguchi S
  • Journal Title: Endocrinol J 54 Pages: 465-470
  • Peer Reviewed
• [Journal Article] Mortality predictors after 10 years of dialysis : a prospective study of Japanese hemodialysis patients (2007)
  • Author(s): Ajiro J, Alchi B, Narita I, Omori K, Kondo D, Sakatsume M, Kazama JJ, Akazawa K, Gejyo F
  • Journal Title: Clini J Am Soc Nephrol 2 Pages: 653-660
  • Peer Reviewed
• [Journal Article] The making of a bone in vessels : From the soft shell to the hard bone (2007)
  • Author(s): Fukagawa M, Kazama JJ
  • Journal Title: Kidney Int 72 Pages: 533-534
  • Peer Reviewed
• [Journal Article] Decreased levels of circulating alpha2-Heremans-Schmid glycoprotein/Fetuin-A (AHSG) in patients with rheumatoid arthritis (2007)
  • Author(s): Sato H, Kazama JJ, Wada Y, Kuroda T, Narita I, Gejyo F, Gao P, Yamashita H
  • Journal Title: Intern Med 46 Pages: 1685-1691
  • NAID: 130000075946
  • Peer Reviewed
• [Journal Article] SM22alfa : the novel phenotype marker of injured glomerular epithelial cells in anti-glomerular basement membrane nephritis (2007)
  • Author(s): Ogawa A, Sakatsume M, Wang X, Sakamaki Y, Tsubata Y, Alchi B, Kuroda T, Kawachi H, Narita I, Shimizu F, Gejyo F
  • Journal Title: Nephron Exp Nephrol 106 Pages: 77-87
  • Peer Reviewed
• [Journal Article] Japanese Society of Dialysis Therapy treatment guidelines for secondary hyperparathyroidism. (2007)
  • Author(s): Kazama JJ
  • Journal Title: Therapeutic Apheresis and Dialysis 11・S1 Pages: 44-47
• [Journal Article] Decreased level s of circulating alpha2-Heremans-Schmid glycoprotein/Fetuin-A(AHSG)in patients with rheumatoid arthritis (2007)
  • Author(s): Sato H, et. al.
  • Journal Title: Internal Medicine 46・20 Pages: 1685-1691
• [Journal Article] Mortality predictors after 10 years of dialysis:A Prospective Study of Japanese Hemodialysis Patients (2007)
  • Author(s): Ajiro J, et. al.
  • Journal Title: Clinical Journal of American Society of Nephrology 2・4 Pages: 653-660
• [Journal Article] SM22alpha: the novel phenotype marker of injured glomerular epithelial cells in anti-glomerular basement membrane nephritis. (2007)
  • Author(s): Ogawa A, et. al.
  • Journal Title: Nephron Experimental Nephrology 106・3
• [Journal Article] Oral phosphate bindrs : History and Prospects
  • Author(s): Kazama JJ
  • Journal Title: Bone (in press)
  • Peer Reviewed
• [Presentation] Cancellous Bone Volume is a Histomorphometric Prameter that Indicates Cancellous Bone Connectivity in Chronic Dialysis Patients (2008)
  • Author(s): Kazama JJ, Koda R, Narita I, Gejyo F, Tokumoto A
  • Organizer: 第41回アメリカ腎臓学会
  • Place of Presentation: フィラデルフィア
  • Year and Date: 2008-11-07
• [Presentation] Cancellous Bone Volume is a Histomorphometric Prameter that Indicates Cancellous Bone Connectivity in Chronic Dialysis Patients (2008)
  • Author(s): Kazama JJ, Koda R, Narita I, et al.
  • Organizer: 第41回アメリカ腎臓学会学術集会
  • Place of Presentation: 米国・フィラデルフィア市
  • Year and Date: 2008-11-05
• [Presentation] 日本のガイドラインポリシー (2008)
  • Author(s): 風間順一郎
  • Organizer: 第53回日本透析医学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-21
• [Presentation] 透析患者の胆石保有率は高いが胆石形成リスクは低い (2008)
  • Author(s): 風間順一郎、甲田亮、成田一衛、下条文武
  • Organizer: 第53回日本透析医学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-21
• [Presentation] 日本のガイドラインポリシー (2008)
  • Author(s): 風間順一郎
  • Organizer: 第53回日本透析医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-06-05
• [Presentation] 透析患者の胆石保有率は高いが胆石形成リスクは低い (2008)
  • Author(s): 風間順一郎, 甲田 亮, 成田一衛, 他
  • Organizer: 第53回日本透析医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-06-05
• [Presentation] 生体部分肝移植と腎機能の関連 (2008)
  • Author(s): 風間順一郎、大矢洋、山本智、遠藤裕、佐藤好信、畠山勝義、成田一衛、下條文武
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-06-01
• [Presentation] 生体部分肝移植と腎機能の関連 (2008)
  • Author(s): 風間順一郎, 大矢洋, 山本智, 他
  • Organizer: 第51回日本腎臓学会学術集会
  • Place of Presentation: 福岡市
• [Presentation] Medical management of vascular calcification (2007)
  • Author(s): Kazama JJ
  • Organizer: 第40回アメリカ腎臓学会サテライトシンポジウム
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2007-11-05
• [Presentation] Vascular calcification : Is it ectopic calcification or ectopic ossification? (2007)
  • Author(s): Fukagawa M, Kazama JJ
  • Organizer: 第40回アメリカ腎臓学会サテライトシンポジウム
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2007-11-05
• [Presentation] Titamium Oxide-like compound (TAP) is a promissing material for novel phosphate binder (2007)
  • Author(s): Kazama JJ, Tamagawa K, Narita I, Gejyo F, Konishi Y, Imaoji H, Wakioto S, Kuwahara T
  • Organizer: 第40回アメリカ腎臓学会総会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2007-11-03
• [Presentation] High prevalence of gallstone in end-stage CKD patients is derived from increased risk of gallstone formation in early CKD stages (2007)
  • Author(s): Kazama S, Kazama JJ, Yamamoto S, Narita I, Gejyo F, Shimotori T
  • Organizer: 第40回アメリカ腎臓学会総会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2007-11-03
• [Presentation] 低回転骨は悪いのか? (2007)
  • Author(s): 風間順一郎
  • Organizer: 第52回日本透析医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-17
• [Presentation] Humoral factors that possible involve with the development of vascular calcification in uremia (2007)
  • Author(s): Kazama JJ
  • Organizer: 第52回日本透析医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-16
• [Presentation] 血清Pと血清Caをどうやって目標値にもっていくか? (2007)
  • Author(s): 風間順一郎
  • Organizer: 第52回日本透析医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-16
• [Presentation] 金コロイド比色法による血清シスタチンC濃度測定の有用性 (2007)
  • Author(s): 佐藤弘恵、風間順一郎、黒田毅、成田一衛、中野正明、下條文武
  • Organizer: 第50回日本腎臓学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2007-05-26
• [Presentation] カルシトリオール治療開始後の血清リン濃度の早期変化は治療の長期成績と関連する (2007)
  • Author(s): 風間順一郎、保坂聖子、山本卓、伊藤由美、丸山弘樹、成田一衛、下條文武
  • Organizer: 第50日本腎臓学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2007-05-25
• [Presentation] Three Dimensional Quantitative Analysis of Trabecular Bone Connectivity in Uremic Bone (2007)
  • Author(s): Kazama JJ, Tokumoto A, Yamamoto S, Maruyama H, Gejyo F
  • Organizer: 国際腎臓学会2007サテライトシンポジウム
  • Place of Presentation: アングラドスレイ
  • Year and Date: 2007-04-27
• [Presentation] Septic Multiple Organ Dysfunction Syndrome Causes Hypercalcemia Through Abnormal Vitamin D Metabolism (2007)
  • Author(s): Kazama JJ, Sato N, Hida S, Yamamoto S, Endo H, Narita I, Gejyo F
  • Organizer: 国際腎臓学会2007
  • Place of Presentation: リオデジャネイロ
  • Year and Date: 2007-04-24
• [Presentation] 副甲状腺機能の管理と骨代謝の評価 (2007)
  • Author(s): 風間順一郎
  • Organizer: 日本泌尿器科学会第95回学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-17
• [Book] CKD-MBDハンドブック (2009)
  • Author(s): 風間順一郎
  • Publisher: 医薬ジャーナル、大阪
• [Book] 腎不全ハンドブック (2009)
  • Author(s): 風間順一郎
  • Total Pages: 107
  • Publisher: 医薬ジャーナル、大阪
• [Book] 最新透析医学 (2008)
  • Author(s): 風間順一郎
  • Publisher: 医薬ジャーナル、大阪
• [Book] 最新透析医学 (2008)
  • Author(s): 西洋 良記, 風間順一郎, 他
  • Total Pages: 734
  • Publisher: 医薬ジャーナル
• [Book] 臨床検査項目辞典 (2008)
  • Author(s): 風間順一郎、ほか
  • Total Pages: 836
  • Publisher: 医歯薬出版
• [Book] 血液浄化療法ハンドブック改訂第4版 (2007)
  • Author(s): 風間順一郎
  • Publisher: 協同医書出版社、東京
• [Book] 透析患者の検査値の読み方改訂第2版 (2007)
  • Author(s): 風間順一郎
  • Publisher: 日本メディカルセンター、東京
• [Book] 慢性腎臓病に伴う骨ミネラル代謝異常(CKD-MBD) (2007)
  • Author(s): 風間順一郎
  • Publisher: 医薬ジャーナル、大阪
• [Book] EBM透析療法 (2007)
  • Author(s): 風間順一郎
  • Publisher: 中外医学社、東京
• [Book] 血液浄化療法ハンドブック (2007)
  • Author(s): 風間順一郎、ほか
  • Total Pages: 379
  • Publisher: 協同医書出版
• [Book] 透析患者の検査値の読み方 (2007)
  • Author(s): 風間順一郎、ほか
  • Total Pages: 437
  • Publisher: 日本メディカルセンター
• [Book] 慢性腎臓病に伴う骨ミネラル代謝異常(CKD-MBD) (2007)
  • Author(s): 風間順一郎、ほか
  • Total Pages: 343
  • Publisher: 医薬ジャーナル
• [Book] EBM透析療法 (2007)
  • Author(s): 風間順一郎、ほか
  • Total Pages: 556
  • Publisher: 中外医学社