Study for physiological function of metastin/kisspeptin with special reference to reproduction.
| Project/Area Number |
19390252
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Saitama University |
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Principal Investigator |
INOUE Kinji Saitama University, 理工学研究科, 教授 (50091963)
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| Co-Investigator(Kenkyū-buntansha) |
足立 明人 埼玉大学, 理工学研究科, 准教授 (20351588)
冨田 幹夫 埼玉県立がんセンター, 臨床腫瘍研究所, 専門員 (80142115)
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| Co-Investigator(Renkei-kenkyūsha) |
冨田 幹夫 (80142115)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | メタスチン / キスペプチン / 生殖制御 / 時計遺伝子 / がん転移 / 前腹側室周囲核(AVPV) / 視床下部弓状核(ARC) / エストロゲン / LHサージ / アポトーシス / Metastin / AVPV / 弓城核 / GABA / 転移 / 末梢組織 / 脳内分布 |
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| Research Abstract |
We previously found that metastin/kisspeptin is produced in anteroventral periventricular nucleus(AVPV) and arcuate nucleus(Arc.) in the hypothalamus. It is also revealed that metastin gene (kiss-1) expression in AVPV is positively and Arc is negatively regulated by estrogen and may be involved in ovulation and gonadal development through the surge or pulse mode release of GnRH/LH. In this study we found a clock gene which regulates metastin gene expression. The clock gene (Dbp) shows not only additive effects with E2 and it's nuclear receptor ERa on the expression of metastin, but correlation of spatio-temporal expression pattern between Dbp and metastin. These results strongly suggest Dbp is key regulator, which set a time of LH surge.
In this study we also performed immunocytochemical study for metastin and GnRH neurons and found that metastin and GnRH nerve fibers closely contacted at median eminence, which strongly suggest that metastin may regulate GnRH neurons in median eminence. On the other hand, metastin is found as a peptide which suppresses metastasis of cancer cells however, the effect of metastin on metastasis is not well studied. In this study we found that metastin activates ERK1/2 gene in many kind of cancer cells. We also found that metastin has no effect on the cancer cell proliferation and apoptosis however, it inhibited migration of a mammary carcinoma and a lung carcinoma cells.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Involvement of anteroventral periventricular metastin/kisspeptin neurons in estrogen positive feedback action on luteinizing hormone release in female rats2007
Author(s)
Adachi S, Yamada S, Takatsu Y, Matsui H, Kinoshita M, Takase K, Sugiura H, Ohtaki T, Matsumoto H, Uenoyama Y, Tsukamura H, Inoue K, Maeda K.
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Journal Title
J Reprod Dev 53
Pages: 367-78
NAID
Related Report
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