Identification of novel auto-antibodies and corresponding antigens in patients with aplastic anemia using CLIP replacement Ii chain library

• Project/Area Number: 19390260
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kanazawa University
• Principal Investigator: NAKAO Shinji Kanazawa University, 医学系, 教授 (70217660)
• Co-Investigator(Kenkyū-buntansha): TAKAMI Akiyoshi 金沢大学, 附属病院, 准教授 (80324078) ; KONDO Yukio 金沢大学, 附属病院, 助教 (10322116) ; SENJYU Satoru 熊本大学, 大学院・医学薬学研究部, 准教授 (50274709) ; YAMAZAKI Hirihito 金沢大学, 附属病院, 助教 (50361994) ; TAKAMATSU Hiroyuki 金沢大学, 医学系, 協力研究員 (70401932)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2007: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 再生不良性貧血 / hnRNPK / DRS-1 / moesin / 免疫抑制療法 / 自己抗体 / モエシン / 慢性関節リウマチ

Research Abstract

再生不良性貧血(再不貧)における新たな自己抗原を同定するため、造血幹細胞由来の蛋白を再不貧患者血清と反応させたところ、heterogenous nuclear ribonucleoprotein(hnRNPK)に対する抗体が同定された。この抗hnRNP抗体は自己免疫性再生不良性貧血(再不貧)で高頻度に検出されることが明らかになった。この抗体は再不貧患者の約30%に検出され、陽性患者では免疫抑制療法に対する反応性が、陰性患者に比べて有意に高かった。したがって本抗体は再不貧における免疫病態の良いマーカーと考えられた。

Research Products

• [Journal Article] Aberrant increase in the immature platelet fraction in patients with myelodysplastic syndrome : a marker of karyotypic abnormalities associated with poor prognosis Eur J Haematol (2009)
  • Author(s): Sugimori N, Kondo Y, Shibayama M, Omote M, Takami A, Sugimori C, Ishiyama K, Yamazaki H, Nakao S
  • Journal Title: 82 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Anti-moesin antibodies in the serum of patients with aplastic anemia stimulate peripheral blood mononuclear cells to secrete TNF-alpha and IFN-gamma (2009)
  • Author(s): Takamatsu H, Espinoza JL, Lu X, Qi Z, Okawa K, Nakao S
  • Journal Title: J Immunol 182 Pages: 703-710
  • Peer Reviewed
• [Journal Article] Aberrant increase in the immature platelet fraction in patients with myelodysplastic syndrome : a marker of karyotypic abnorm alities associated with poor prognosis. (2009)
  • Author(s): Sugimori N, et al
  • Journal Title: Eur J Haematol 82 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Anti-moesin antibodies in the serum of patients with aplastic anemia stimulate peripheral blood mononuclear cells to secrete TNF-alpha and IFN-gamma (2009)
  • Author(s): Takamatsu H, et al
  • Journal Title: J Immunol 182 Pages: 703-710
  • Peer Reviewed
• [Journal Article] Expansion of donorderived hematopoietic stemcells with PIGA mutation associated with late graft failure after allogeneic stem cell transplantation (2008)
  • Author(s): Mochizuki K, Sugimori C, Qi Z, Lu X, Takami A, Ishiyama K, Kondo Y, Yamazaki H, Okumura H, Nakao S
  • Journal Title: Blood 112 Pages: 2160-2162
  • Peer Reviewed
• [Journal Article] Expansion of donorderived hematopoietic stem cells with PIGA mutation associated with late graft failure after allogeneic stem cell transplantation. (2008)
  • Author(s): Mochizuki K, at al
  • Journal Title: Blood. 112 Pages: 2160-2162
  • Peer Reviewed
• [Journal Article] Roles of DRB1 *1501 and DRB1 *1502 in the pathogenesis of aplastic anemia (2007)
  • Author(s): Sugimori C, Yamazaki H, Feng X, Mochizuki K, Kondo Y, Takami A, Chuhjo T, Kimura A, Teramura M, Mizoguchi H, Omine M, Nakao S
  • Journal Title: Exp Hematol 35 Pages: 13-20
  • NAID: 120000809590
  • Peer Reviewed
• [Journal Article] Treatment of severe aplastic anemia with antithymocyte globulin and cyclosporin A with or without G-CSF in adults : a multicenter randomized study in Japan (2007)
  • Author(s): Teramura M, Kimura A, Iwase S, Yonemura Y, Nakao S, Urabe A, Omine M, Mizoguchi H
  • Journal Title: Blood 110 Pages: 1756-1761
  • Peer Reviewed
• [Journal Article] Cyclosporine therapy for acquired aplastic anemia : predictive factors for the response and long-term prognosis (2007)
  • Author(s): Yamazaki H, Sugimori C, Chuhjo T, Nakao S.
  • Journal Title: Int J Hematol 85 Pages: 186-190
  • NAID: 10019488749
  • Peer Reviewed
• [Journal Article] Roles of DRB1*1501 and DRB1*1502 in the pathogenesis of aplastic anemia. (2007)
  • Author(s): Sugimori, C., et. al.
  • Journal Title: Exp Hematol 35 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Treatment of severe aplastic anemia with antithymocyte globulin and cyclosporin A with or wlthout G-CSF in adults a multicenter randomized study in Japan. (2007)
  • Author(s): Teramura, M, et. al.
  • Journal Title: Blood 110 Pages: 1756-1761
  • Peer Reviewed
• [Journal Article] Cyclosporine therapy for acquired aplastic anemia: predictive factors for the response and long-term prognosis. (2007)
  • Author(s): Yamazaki, H., et. al.
  • Journal Title: Int J Hematol 85 Pages: 186-190
  • NAID: 10019488749
  • Peer Reviewed
• [Presentation] Autoantibodies specific to hnRNP K : a new diagnostic marker for immune pathophysiology in bone marrow failure syndromes. (2008)
  • Author(s): Zhirong Qi, Nakao S, et al
  • Organizer: 第50回アメリカ血液学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-12-07
• [Presentation] Autoantibodies specific to hnRNP K : a new diagnostic marker for immune pathophysiology in bone marrow failure syndromes (2008)
  • Author(s): Zhirong Qi, Nakao S, et.al.
  • Organizer: The American Society of Hematology 50^th Annual Meeting
  • Place of Presentation: San Francisco, CA,USA
• [Presentation] Donor-Cell Derived Aplastic Anemia (AA) with a Small Population of CD55-CD59-Blood Cells after Allogeneic Stem Cell Transplantation : Evidence for the Immune System Attack Against Normal Hematopoietic Stem Cells in AA (2007)
  • Author(s): Sugimori, C, Nakao S, et.al.
  • Organizer: The American Society of Hematology 49^th Annual Meeting
  • Place of Presentation: Atlanta, Georgia, USA
• [Presentation] Donor-Cell Derived Aplastic Anemia (AA) witha Small Population of CD55-CD59-Blood Cells after Allogeneic Stem Cell Transplantation: Evidence for the Immune System Attack Against Normal Hematopoietic Stem Cells in AA. (2007)
  • Author(s): Sugimori, C., et. al.
  • Organizer: The American Society of Hematology 49th Annual Meeting
  • Place of Presentation: Atlanta, Georgia, USA